Webinars and Online Training - Products

The objective of this webinar is to guide GC users on GC column care & troubleshooting. Audience will learn about the ways to maintain GC column performance. Guidance to troubleshoot common issues in GC separation will be share in this webinar.

This is the second in our series of webinars on fundamental concepts in gas chromatography. This session will cover: Different types of GC columns, The different column dimensions and their relevance, Column phases and polarity, Column temperature ranges.

The objective of this webinar is to guide LC users on LC column care & troubleshooting. Audience will learn about ways to maintain LC column performance and prolong column lifetime. Guidance to troubleshoot issues caused by LC column will be share in this webinar.

Inductively coupled plasma mass spectrometry (ICP-MS) is an analytical technique that can be used to measure elements at trace levels in a large variety of biological and environmental samples (e.g., fluids, tissues, seeds, soil, water). Although older techniques such as atomic absorption and atomic emission are still in use by some laboratories, there has been a slow shift toward ICP-MS, particularly in the last decade. From a laboratory perspective, the most important advantage of ICP-MS is the capability to accurately measure trace levels of a wide range of elements simultaneously. Coupled with short analysis time and straightforward sample preparation, this analytical technique offers very high sample throughput in the laboratory. Nutrient levels of chloride, copper, iodine, manganese, iron, selenium, and zinc can be measured in soil, water, plants, as well as human and animal samples. At the same time, toxic levels of metals like arsenic, aluminum, barium, chromium, cadmium, mercury, lead, and nickel can easily be detected and measured quantitatively. This is important in clinical laboratories, environmental research and risk assessments, human and animal health and nutrition, pharmaceutical research and development as well as manufacturing (cGMP), soil and plant health and nutrition, microbiome research, and biopesticide research and development, to name a few.

AA or ICP-OES/MS is often viewed as the golden standard to analyze elements during the pharmaceutical manufacturing process, from drug discovery to the QA/QC of final formulations. While extremely accurate, these techniques have the downside of requiring the sample to be in liquid form when analyzing, resulting in a very arduous sample-preparation process and long analysis time. In this seminar, we would introduce Energy-Dispersive X-Ray Fluoroscopy (EDX) as an alternative technique to perform elemental analysis that requires little sample preparation, is easy to use and can provide results quickly; and how it could be used in various areas of the pharmaceutical industry.

24 targeted elemental impurities in three drug products were determined following new USP chapters <232>/<233> using Shimadzu ICPMS-2030. The method was evaluated and validated according to the procedures defined by USP <233> and combined with microwave digestion for sample preparation. Good calibration linearity, repeatability, stability and specificity were achieved for all 24 impurities. Low LODs and LOQs were obtained as compared to the target limits from USP <232>. The ICP-MS method applying universal He collision mode provides a high throughput method for routine elemental impurity analysis in pharmaceutical products.

This series is perfect for anyone currently using GC, who'd like to understand more about the technique, the hardware and the data produced. It's also suitable for anyone who is new to GC and who'd like to understand more about it.

This series is perfect for anyone currently using GC, who'd like to understand more about the technique, the hardware and the data produced. It's also suitable for anyone who is new to GC and who'd like to understand more about it. This webinar is designed to overview of the selection of the most common sampling technique outside of liquid injection.

In this webinar, we will discuss the fundamentals of GC-SCD as well as common challenges, explore the unique design of Shimadzu’s new SCD and how it overcomes these challenges, and present data for this system’s applicability to ASTM D5504, D5623, D7011, as well as quantification of sulfur compounds in ethylene and propylene.

The objective of this webinar is to guide GC users on GC column care & troubleshooting. Audience will learn about the ways to maintain GC column performance. Guidance to troubleshoot common issues in GC separation will be share in this webinar.

This is the first of a series of webinars on fundamental concepts in gas chromatography. This first session will cover: Principles of gas chromatography, Uses of gas chromatography, Hardware overview, and Carrier gases. This series is perfect for anyone currently using GC, who'd like to understand more about the technique, the hardware and the data produced. It's also suitable for anyone who is new to GC and who'd like to understand more about it.

This is the second in our series of webinars on fundamental concepts in gas chromatography. This session will cover: Different types of GC columns, The different column dimensions and their relevance, Column phases and polarity, and Column temperature ranges. This series is perfect for anyone currently using GC, who'd like to understand more about the technique, the hardware and the data produced. It's also suitable for anyone who is new to GC and who'd like to understand more about it.

This session is part of our series of webinars on fundamental concepts in gas chromatography. This session will cover: The GC inlet, The split/splitless inlet, Split mode, Splitless mode (including high-pressure or pulsed splitless injection), and Carrier gas saver. This series is perfect for anyone currently using GC, who'd like to understand more about the technique, the hardware and the data produced. It's also suitable for anyone who is new to GC and who'd like to understand more about it.

This session is part of our series of webinars on fundamental concepts in gas chromatography. This session will cover: When split/splitless inlets are not suitable, Programmable Temperature Vaporisation (PTV), On-Column Injection (OCI) or Cool On-Column (COC), and Multi-Mode Inlets (MMI). This series is perfect for anyone currently using GC, who'd like to understand more about the technique, the hardware and the data produced. It's also suitable for anyone who is new to GC and who'd like to understand more about it.

This session is part of our series of webinars on fundamental concepts in gas chromatography. This session will cover: Headspace, Thermal desorption, SPME, Pyrolysis, and Gas Sampling. This series is perfect for anyone currently using GC, who'd like to understand more about the technique, the hardware and the data produced. It's also suitable for anyone who is new to GC and who'd like to understand more about it.

This session is part of our series of webinars on fundamental concepts in gas chromatography. This session will cover GC Detectors: Choice of detectors available (incl. FID, TCD, BID, ECD, FPD, SCD & NPD), Detector sensitivity & selectivity, and Detector requirements. This series is perfect for anyone currently using GC, who'd like to understand more about the technique, the hardware and the data produced. It's also suitable for anyone who is new to GC and who'd like to understand more about it.

This is the seventh session in a series of webinars on fundamental concepts in gas chromatography. This session will cover Data Processing: Qualitative analysis, Quantitative analysis, Sample types (standards, controls, etc.), and Compound types (targets, surrogates, internal standards, etc.). This series is perfect for anyone currently using GC, who'd like to understand more about the technique, the hardware and the data produced. It's also suitable for anyone who is new to GC and who'd like to understand more about it.

This is the eighth session in a series of webinars on fundamental concepts in gas chromatography. This session will cover Maintenance & Troubleshooting: Inlet maintenance, Column maintenance & installation, and Detector maintenance. This series is perfect for anyone currently using GC, who'd like to understand more about the technique, the hardware and the data produced. It's also suitable for anyone who is new to GC and who'd like to understand more about it.

This is the ninth & final session in a series of webinars on fundamental concepts in gas chromatography. This session will cover Method Development: Things to consider, Carrier gas selection, Detector selection, Liner choice, Oven program, and Derivatisation. This series is perfect for anyone currently using GC, who'd like to understand more about the technique, the hardware and the data produced. It's also suitable for anyone who is new to GC and who'd like to understand more about it.

The price of helium is soaring and deliveries can be erratic! Learn how to save time and money in your lab by reducing helium consumption or swapping it for a different carrier gas entirely!

With helium prices on the rise and shortages looming, people everywhere are looking for an alternative. Join us Tuesday February 4th at 1pm EST to discuss the advantages and disadvantages of hydrogen as a carrier gas. We will cover everything from ease of switching to how your GC and GCMS results will be affected. We will also have a special guest from Peak Scientific joing us to discuss using generators!

In this new digital world, Artificial Intelligence (AI) becomes essential to simplify workflow and increase efficiency. New Nexera GPC System, which comes with Analytical Intelligence (AI) provides highly reliable analytical results by preventing potential problems and ensuring the instrument is always in a stable state, regardless of the skill, knowledge, or experience level of the analyst. In this webinar, our speakers will introduce New Nexera GPC hardware and software includes analytical workflow automation and analytical intelligence (AI). Speakers will give an introduction of detectors selection for the GPC system and GPC columns selection.

This is the second in our series of webinars on fundamental concepts in gas chromatography. This session will cover: Different types of GC columns, The different column dimensions and their relevance, Column phases and polarity, Column temperature ranges.

This is the first of a series of webinars on fundamental concepts in gas chromatography. This first session will cover: Principles of gas chromatography, Uses of gas chromatography, Hardware overview, Carrier gases.

Many companies will soon be reopening and allowing staff to get back into their laboratories. There are going to be several challenges for companies to consider, during this transition period, but most are related to health & safety or financial hurdles. By listening to this webinar, you will learn many specific approaches labs can utilize with existing products as well as new features that can help in both of these categories. Things like automated sample preparation and remote control/monitoring of your instrumentation aid in social distancing efforts. Additionally, utilizing new features in gas selection can help save money and automate processes like never before.

The determination of water content in alcoholic beverages, solvents and various consumer products such as foods, fuels, pharmaceuticals, and petroleum products is one of the most common types of chemical testing. Many techniques such as gravimetric analysis, gas chromatography (GC), Karl Fischer Titration, near infra-red spectroscopy, and others have been used for water quantification with good results. However, the limitations of these approaches can include high limits of detection, large sample sizes required for trace analysis, side reactions, the use of expensive consumables, and the production of chemical waste. Previous work by Prof. Daniel Armstrong and co-workers has described the use of ionic liquid GC capillary columns for the trace analysis of the water content in a wide variety of solvents. These columns were capable of providing a rapid and quantitative determination of water contents using very small sample amounts. This webinar further examines the characteristics of ionic liquid capillary columns for the GC analysis of water.

This eLearning session introduces the operating principle of the SCD, as well as the innovations brought by Shimadzu to improve the productivity and minimize the instrument downtime. You will learn the various features of Shimadzu SCD which make it a reliable tool for analysing sulfur-containing compounds down to the trace level.

This webinar introduces Shimadzu's Next Industry Standard GC, Nexis GC-2030. The Shimadzu's premier gas chromatograph offers a modern approach to a classic chromatographic technique. Designed with the user in mind, new innovative features, exceptional performance and high-throughput capabilities will elevate your lab to the next level.

Simultaneous measurement of total siloxanes in biogas presents several challenges including a wide spectrum of sample matrices, challenging sensitivity requirements, and many sources of sample contamination. The newly developed standard method for sampling and analysis of siloxanes in gaseous fuels is ASTM D8230. In its current form, the method prescribes sampling by tedlar bags, passivated canisters, and sorbent traps with several options for laboratory analysis including GC-MS, GC-ICP-MS, or GC-AED.. In an effort to improve the method, Ohio Lumex has developed a siloxane sorbent trap and analysis procedure using a Shimadzu GCMS-QP2020 NX with TD-30 attachment. Ohio Lumex has also developed other methods allowed by ASTM D8230 on the same instrument to directly compare method performance. This procedure, as well as the advantages it provides over the current procedures described in D8230, will be discussed.

Passenger and commercial vehicle tires are a complex materials technology that may include ten or more distinct layered rubber compounds each formulated to provide different physical and chemical properties based on application and end use. These compounds are engineered to meet specific metrics related to weight, rolling resistance, and wet traction so as to comply with strict fuel economy and road safety regulations. Therefore, information obtained for the purposes of reverse engineering and materials analysis is of great value to tire chemists especially in the case of failure investigation. Each rubber material is composed of numerous chemical components such as small molecular compounds (plasticizer oils, anti-degradants, and curatives), oligomers and polymers (reinforcing resins, rubber, and rubber blends), and inorganic fillers (carbon black, silica, clay, etc.). Because these materials are complex and generally vulcanized analysis can be time consuming and difficult. Py-GC/MS is a simple and effective method to quickly break down materials for whole sample analysis. This presentation examines the use of double shot Py-GC/MS in the tire industry for materials characterization and formula reconstruction as compared to traditional methods.

Essential oils are one of the growing commodity markets in the flavor fragrance and aromatherapy sector. Its demand is increasing since it has fewer side effects than synthetic medicines. Unfortunately, due to a lack of reliable instrumental techniques and strict government regulation, adulteration of natural products is rampant worldwide: adulteration impacts the therapeutic values of oils and a country’s economy. Tentatively 80% of commercially available, so-called “natural” essential oils are adulterated somehow. Chiral GCMS is one of the most useful and practical applications for the authentication of essential oils. Plants of the specific species from any origins produce the same enantiomeric ratios, which is a crucial indicator of authentication in essential oils. For example, lavender has 95% L linalool, whereas coriander has 84% D linalool, citrus has 99% D limonene, fir needle has 100% L-limonene. Even being the same physicochemical properties, they have distinctly different biological and organoleptic properties. The enantiomer of the same molecule at the same time can be harmless as well as toxic. A chiral GCMS analysis helps detect other species mixing or nature-identical synthetic compound addition in essential oils.

Wine is a very delicate agricultural product, whereby, similar grape varieties can achieve very different aromatic profiles due to various cultivation conditions. With these variations, aroma profiling of wine is essential in the production process to identify consumers' preferences, formulating conditions and also quality control. These aromatic profiles are determined by a multitude of volatile compounds which are usually characterized comprehensively using GC/GCMS. In this webinar, Prof. Erich Leitner from Graz University of Technology will share on wine profiling and how GC/GCMS as analytical tool could help on different origin of different wines.

Have you ever wanted an alternate sample introduction method? Maybe the sample of interest needs to be hotter than a standard inlet will reach. Pyrolysis is a sample introduction technique that uses elevated temperatures to break down organic materials. The starting samples can be anything from liquids to solids from many different application areas. Attend to get your questions answered by the experts.

The presentation will provide fundamentals of GC-MS-MS, and what this technique 'brings to the table' over and above what is achievable by GC-MS. The talk will then go on to describe examples of transitioning from GC-MS to GC-MS-MS including steroids from water, fatty acid methyl esters, and pesticides. Attendees to this presentation will learn about the questions they should be asking as they consider transitioning analytical methods and workflow from GC-MS to GC-MS-MS.

The price of helium is soaring and deliveries can be erratic. Learn how to save time and money in your lab by reducing your GCMS system's helium consumption or swapping your GCMS carrier gas to hydrogen or nitrogen!

With helium prices on the rise and shortages looming, people everywhere are looking for an alternative. Join us to discuss the advantages and disadvantages of hydrogen as a carrier gas. We will cover everything from ease of switching to how your GC and GCMS results will be affected. We will also have a special guest from Peak Scientific joing us to discuss using generators!

This webinar introduces Shimadzu's latest solutions for chemical ionization technique with GC/MS. Three innovative solutions for chemical ionization will be introduced. Join us to learn about how seamless it is to adopt solvent-based chemical ionization technique in your current workflow with the solvent-mediated chemical ionization (SMCI) system. In addition, get to know how an innovative direct sampling tool and a unique Smart EI/CI ion source are capable of enabling consecutive electron and chemical ionization to supercharge your GC/MS analysis.

Formation of nitrosamines, particularly NDMA, is prevalent in water treatment plants using chloramine as a disinfectant. NDMA is classified as a B2 carcinogen based on the induction of tumors at multiple sites in different mammal species exposed to NDMA by various routes. EPA 521 has been provided as the validated extraction and analytical procedure for monitoring of nitrosamines in drinking water. However, this method was developed using large volume injections (20 µL) and ion trap spectrometers in MS/MS chemical ionization (CI) mode (with methanol or acetonitrile). To avoid the complexity of large volume injections and CI, we present the alternative gas chromatography triple quadrupole electron ionisation method. Additionally, calibrants were prepared via an auto-sampler, from 1000 µg/ml commercially available standards.

Nitrosamine compounds are classified as probable human carcinogens by the International Agency for Research on Cancer (IARC). Ever since the first U.S. Food and Drug Administration (FDA) notification in June 2018 about levels of these impurities in pharmaceutical API, they have been a reason of continuous discussion globally for analytical scientist, patients and regulators. After June 2018, the number of recalls and 483’s have been on a rise for not only angiostenin II receptor blockers (-ARB, also called as sartans) but also for other products like Ranitidine, Metformin etc. FDA, European Medicine Agency (EMA) and International Council for Harmonisation (ICH) have constantly set and update the guidelines in measuring impurities in pharmaceutical products. Holistic solution to measurement of nitrosamines need both GC-MS/MS and LC-MS/MS technologies.

The following webinar talks about introduction to Genotoxic impurities, current regulations, Shimadzu solution with respect to method development workflow for GTI’s & well-developed applications for quantitation of GTI’s emphasising more on Nitrosamine quantitation in Valsartan & Metformin along with few other applications. It also discusses Shimadzu’s GCMS configurations and accessories as a complete solution for Pharmaceutical analysis.

In this event, our two speakers will discuss the suitability of this technique for assessing the fate of microplastics in the environment. They will also present examples of how they have applied Py-GCMS for studying these contaminants in water, biosolids and seafood samples, among others.

During this short webinar, we will look at why pyrolysis is important and the different areas of use, discuss method mapping using a pyrolyzer and finally how it can be used for day to day material characterization.

Join us for the second in a three part series about PY-GC/MS. In this webinar presented by Shimadzu, we will be looking at a few applications discussed in our previous webinar as well as the use of some additional accessories for the pyrolyzer. While many think of the pyrolyzer as a technique used for plastics identification only, there are many other application spaces. These units can be for characterization, composition, and identification in everything from plastics and polymers to foods and consumer products and so much more.

During this webinar, we will have a discussion about using pyrolysis as an everyday technique, and you will have the opportunity to ask your questions to our expert panel from Shimadzu Scientific Instruments, Frontier Laboratories and Cooper Tire & Rubber Co.

Join us for our first Forensics Webinar! We will be covering the rapidly growing field of Arson as it pertains to TD-GCMS. Come and learn everything you need to know regarding Arson, and Fire Debris and how Shimadzu GCMS paired with thermal desorption will burn through the competition!

This webinar provides a glimpse of the cutting-edge technology Shimadzu GCMSMS offers in the field of food safety. Agricultural industry faces a huge challenge of producing increasing quantities of food. While meeting such expanding global food demand, the safety and sustainability of food supply is of utmost importance. With the use of chemicals, significant reduction in crop losses and thus increased yield has been achieved. However, the resulting pesticide residues is of growing concern with its potential health risks. The exponential growth of chemical hazards and their regulations have brought several challenges to the food safety industry. It is particularly challenging for laboratories to accurately analyse large amounts of samples and detect the non-compliant ones. Understanding current laboratory requirements, GCMS-NX series along with its robust and sensitive hardware provides simplified user-friendly software for handling large multi-component data. Along with the unmatched hardware performance, Shimadzu also offers customizable databases and method packages as a total solution.

Forensic chemists face the challenge of identifying new psychoactive substances (NPS), whereby the general structures of controlled drugs are modified to evade legislative banning. Hence, a plethora of synthetic cathinones and cannabinoids has been available to the public, causing a global social problem. It is therefore crucial that analytical methods are developed for identifying and differentiating new analogues of synthetic drug substances, to prevent potential trading and abuse. Additionally, drug legislation is made more challenging with the use of synthetic cannabinoid containing products, typically marketed as “herbal mixtures”, which consists of analogues of synthetic cannabinoids. In this study, simultaneous multi-reaction monitoring (MRM) and product ion scan (PIS) acquisition methods have been developed for detection and differentiation of isomeric synthetic cannabinoids.

Analysis of the chemical components of fuels is critical to ensuring their quality, combustion characteristics, and compliance with environmental regulations. This webinar will provide a background on the common industry test methods for fuels, the challenges associated with long-standing techniques, and explore Shimadzu’s HPLC-based solutions for the energy industry.

This series is perfect for anyone currently using Preparative LC, who'd like to understand more, as well as those new to the technique or eager to learn more.

Find out how to unlock the full potential of your Photodiode Array (PDA) detector and learn how PDA Peak Deconvolution can detect and characterize unresolved peaks, improve integration, and increase throughput. We will discuss the basis of the technique, accuracy of results, and use cases. Objectives: Understand the difference between 2D and 3D UV-Vis absorbance data. Define MCR-ALS deconvolution and how it applies to photodiode array data. Describe how PDA deconvolution can benefit analytical method development.

The objective of this webinar is to guide LC users on LC column care & troubleshooting. Audience will learn about ways to maintain LC column performance and prolong column lifetime. Guidance to troubleshoot issues caused by LC column will be share in this webinar.

This first session will cover: what is LC purification and how do you get the most out of the technique, how much sample can you expect to purify, up-scaling basics, recycling and concentration. This series is perfect for anyone currently using Preparative LC, who'd like to understand more, as well as those new to the technique or eager to learn more.

This second session will cover: Differences between Semi-micro and Analytical Scale Prep, Principles of the techniques, Things to consider in semi-micro and analytical scale purification, Instrumentation and software advantages. This series is perfect for anyone currently using Preparative LC, who'd like to understand more, as well as those new to the technique or eager to learn more.

This session will cover: Principles, common issues and tips and tricks of Semi-Preparative Chromatography, UFPLC – Ultra Fast Preparative and Purification LC, Semi-preparative SFC Scale purification. This series is perfect for anyone currently using Preparative LC, who'd like to understand more, as well as those new to the technique or eager to learn more.

This first session will cover the Fundamentals of Liquid Chromatography.

This second session will cover Types of LC Separations as well as what can effect the separation of compounds.

This third session will cover the process of separation and introduce terms such as peak efficiency, retention, selectivity and resolution.

This fourth session will cover the Resolution Equation and how to apply the theory in practice. You will learn how to optimize your LC system to improve efficiency and get the most out advances in silica particles including superficially porous particles

This fifth session will conclude the introduction to liquid chromatography series, by providing an overview of what we have learned over previous sessions. You will recap the key concepts from the previous sessions and ready ourselves for moving onto the advances in liquid chromatography series.

Using Method Scouting Solution with LCs and mobile phase or column switching valves will maximize the method development efficiency. Once the optimal conditions are determined, they can be used for general-purpose analyses and transferred to other departments. Integrated and modular systems for method scouring are demonstrated that are universal for SFC, conventional HPLC, and UHPLC. For the needs of the laboratory, the system can be switched between HPLC, UHPLC, and SFC. During this webinar, participants will see how to accelerate drug development, quality control (QC), and chemistry manufacturing and control (CMC) through a variety of applications, ranging from small molecules to large molecules.

As one of the most prominent innovators in LC technology, Shimadzu has set the New Benchmark in the LC innovation by developing the very first AI-enabled LC system, which allows Chemists to spend less time operating, and handling the instrument, as the instrument with its in-built Analytical Intelligence algorithm enables the system of performing automation workflow. The ultimate objective is to achieve minimized system downtime, optimize efficiency & maximize productivity, through the New Benchmarks in Intelligence, Efficiency and Design.

This webinar gives you an introduction on fully automated pre-column derivatization of the amino acids and microwave digestion method which were adopted to further speed up and increase the analysis efficiency. A Shimadzu Nexera LC-40X3 with photodiode array (PDA) and fluorescence (RF) detectors were employed for the analysis. The Nexera LC-40X3 equipped with automated pre-treatment function allows several derivatization reagents to be aspirated sequentially from multiple vials and mixing within the needle and the sample loop. Microwave digestion procedure was employed to carry out both acid and alkaline hydrolysis in less than 30 minutes, which is much faster in comparison to the conventional hydrolysis up to 24 hours. Fluorescent (RF) detection was used for high sensitivity analysis (nM), while UV detection was compared, which shows its applicability for µM level quantitation of amino acids.

This session will cover: The Differences between Semi-micro and Analytical Scale Prep, Things to consider in semi-micro and analytical scale purification, Instrumentation and software advantages

This session will cover: Principles, common issues and tips and tricks of Semi-Preparative Chromatography, UFPLC - Ultra Fast Preparative and Purification LC, Semi-preparative SFC Scale Purification

Cultivation-independent sequencing techniques have revealed a tremendous diversity of microorganisms on our planet. Although, the majority of microorganisms are not isolated and do not have sequenced genomes. While this applies to all fields of environmental microbiology, it does not prevent research from progressing. Conversely, in the field of subsurface microbiology the dearth of cultured representatives heavily restricts the simplest hypothesis driven experimentation. In this work, we discovered a novel strict anaerobic thermophilic bacterium from a monitoring borehole at a depth of 691 meters below land surface. This borehole is of particular interests as it resides within the US Great Basin, in particular the Nevada National Security Site, underlain by the Death Valley Regional Flow System. The characterized isolate was given the name Thermoanaerosceptrum fracticalcis strain DRI-13T, and the sequenced genome coupled with methylome analysis reveal interesting insights into metabolism/energy conservation, gene regulation, DNA defences, and archival of previous viral aggressions.This microorganism and others have been analysed using HPLC to track substrate utilization and waste product production in order to better understand the syntrophic interactions of subsurface life. The deep biosphere may reveal itself as an untapped resource of microbial biodiversity holding novel solutions to industrial, medical, and potential providing answer to the origins of life.

The advantages of consuming supercritical fluid chromatography are indisputable. SFC can provide sweet improvements in many aspects of challenging separations and chromatographic efficiency. However, SFC systems, both analytical and preparative, have historically been beset with nearly as many sour traits often leaving a bitter first impression. Finally, scientists were able to make their taste buds heard and have addressed these deficiencies through a collaborative development recipe. What has been cooked up is a paradigm shift in comprehensive SFC workflows utilizing state-of-the art technology. Consequently, these advances in SFC technology have enabled the implementation of SFC into the labs of both well-seasoned and less-seasoned scientists who have been awaiting innovative solutions to longstanding problems. The Nexera UC series of supercritical fluid chromatography instrumentation brings SFC to the mainstream consumer, and erases the tepid perceptions espoused by legacy stale technology.

Although originally invented for high-throughput screening of combinatorial libraries, affinity selection-mass spectrometry (AS-MS) is ideal for the discovery of lead compounds from chemically diverse sources such as botanical, fungal and microbial extracts. Based on binding interactions between macromolecular receptors and ligands of low molecular mass, AS-MS facilitates the affinity isolation of pharmacologically active small molecules from complex mixtures for mass spectrometric characterization and identification. AS-MS requires no radiolabels, no UV or fluorescent chromophores, and is compatible with all classes of receptors, enzymes, incubation buffers, cofactors, and ligands. The most successful types of AS-MS include pulsed ultrafiltration (PUF) AS-MS, size exclusion chromatography (SEC) AS-MS, and magnetic microbead affinity selection screening (MagMASS), which differ mainly in their approaches for separating the ligand-receptor complexes from the non-binding compounds in mixtures. After affinity isolation, the ligand(s) from the mixture are characterized using high resolution UHPLC-MS and tandem mass spectrometry on instruments such as the Shimadzu LCMS-9030 QToF MS. Based on HRMS elemental composition and tandem mass spectrometric structural data, the identities of the lead compounds are determined through dereplication by searching on-line databases for known natural products and by comparison with standards. For novel natural products, AS-MS provides UHPLC retention times of active compounds for chromatographic isolation and then complete structure determination using a combination of spectroscopic techniques. Advantages of AS-MS over conventional high-throughput screening are speed, reduced consumption of reagents, compatibility with any receptor and ligand, and the ability to discovery allosteric as well as orthosteric ligands to any receptor, whether or not any ligands are already known.

Steroid hormones are a frequent target of analysis in both basic research and clinical laboratories. LC-MS/MS has become the preferred method of analyzing steroid hormones due to small required sample volumes, ability to multiplex panels of hormones, high throughput, and its improved specificity over immunoassays. This presentation will focus on ways in which the Endocrine Technologies Core at Oregon National Primate Research Center applies LC-MS/MS analysis of steroid hormones to advancing research in HIV/AIDS, reproductive disorders, and contraception. A description of the methods and some of the challenges presented by LC-MS/MS analysis of steroid hormones will be presented. Supported by NIH P51 OD011092 and NIH S10 OD026701.

Evolution of mass spectrometry (MS) hardware, especially ionization method, has drastically changed the landscape of MS applications. Direct Probe Ionization Mass Spectrometer (DPiMS) has the potential to pioneer new types of applications with the new ionization interface. The technique successfully incorporated the best of both worlds; high ionization efficiency of ESI and simplicity of MALDI. This was achieved by using an ultrafine needle to sample an extremely small volume of sample on its surface and subsequently applying high voltage to the needle to imitate an ESI probe. DPiMS is characterized by the microscopic sampling volume and high sensitivity for the amount sampled at a low ng/mL concentration range. For this, DPiMS can be a robust solution for the routine analysis of high-complexity matrices, such as plasma or food extract, as contamination of MS hardware can be perfectly mitigated. Moreover, direct sampling from solid samples causing minimal destruction might enable new applications such as real-time analysis of live tissues or cells.

Therapeutic monoclonal antibody (mAb) biosimilars are booming in Asia and across the world, as well as faces significant challenges in quality control. Peptide mapping plays a critical role in structure characterization of antibody drugs. This webinar will introduce 4 Application News for structure characterization of bevacizumab biosimilar using LCMS-9030 Q-TOF mass spectrometry, including peptide mapping, C-terminal sequencing, disulfide bond analysis, and N-glycan analysis.

This webinar is on LC/MS/MS Method Development and is designed for newcomers to LCMS. This presentation will cover: Some introspection, How you view your analytical system, MS and MS/MS 101, Anatomy of an LC system, Is mass spec 'just a detector'?, Anatomy of an LC-MS(MS) system, Low vs High resolution method development, Advantages and disadvantages of LC-MS(MS), HPLC vs LC-MS(MS) method development

Vitamin B and D are important micronutrients to maintain good health. They are often fortified in various foods such as infant and adult formulae for added health benefits. Over the years, there are many AOAC methods dedicated to the analyses of different vitamin B and vitamin D compounds in infant and adult formulae. In recent years, many AOAC methods use LC-MS/MS to analyse both these vitamins. In this webinar, I will discuss about the different AOAC methods and the sample preparations in the analysis of vitamin B and D. I will also share some of the application development works done closely following AOAC methods using Shimadzu LC-MS/MS.

Nitrosamine compounds are classified as probable human carcinogens by the International Agency for Research on Cancer (IARC). Ever since the first U.S. Food and Drug Administration (FDA) notification in June 2018 about levels of these impurities in pharmaceutical API, they have been a reason of continuous discussion globally for analytical scientist, patients and regulators. After June 2018, the number of recalls and 483’s have been on a rise for not only angiostenin II receptor blockers (-ARB, also called as sartans) but also for other products like Ranitidine, Metformin etc. FDA, European Medicine Agency (EMA) and International Council for Harmonisation (ICH) have constantly set and update the guidelines in measuring impurities in pharmaceutical products. Holistic solution to measurement of nitrosamines need both GC-MS/MS and LC-MS/MS technologies.

This free panel discussion welcomes the expert panelists to provide insights into the use of HRMS across a variety of fields, namely food safety, forensics and clinical research. The discussion will feature how HRMS compares with TQMS, main drivers for implementation, common challenges and opinions on what the future holds.

Nitrosamine compounds are classified as probable human carcinogens by the International Agency for Research on Cancer (IARC). Ever since the first U.S. Food and Drug Administration (FDA) notification in June 2018 about levels of these impurities in pharmaceutical API, they have been a reason of continuous discussion globally for analytical scientist, patients and regulators. After June 2018, the number of recalls and 483’s have been on a rise for not only angiostenin II receptor blockers (-ARB, also called as sartans) but also for other products like Ranitidine, Metformin etc. FDA, European Medicine Agency (EMA) and International Council for Harmonisation (ICH) have constantly set and update the guidelines in measuring impurities in pharmaceutical products. Holistic solution to measurement of nitrosamines need both GC-MS/MS and LC-MS/MS technologies.

This webinar will walk through how the new IonFocus system can make a real difference to LC-MS/MS workflows and headline new software capabilities in making data review easier and quicker. Find out how this next step in LC-MS/MS could make a real difference to your lab.

The webinar describes various metrics that are relevant to statistical interpretation of MMA, which explain why mass resolution of 30,000 is more than sufficient for mass measurement at sub-ppm accuracy. Using the Shimadzu LCMS-9030 quadrupole time-of-flight MS, the rationale and strength of instrument stability for consistently achieving sub-ppm MMA has been demonstrated in small molecules.

In this webinar we will summarize how a MALDI-imaging experiment is performed and discuss the most critical steps to a successful experiment. We will also offer a practical example, demonstrating how high spatial resolution MALDI-imaging is being used to identify previously unobserved changes in the lipidome of human colon in the context of inflammatory bowel disease, which provides new insights into the mechanisms of this disease.

This webinar covers topics including the origins of the digital ion trap, ion trap vs digital ion trap, Shimadzu's journey to the MALDI digitial ion trap, basic proteomics, glycomics, antibody drug conjugates, and more.

The COVID-19 pandemic has brought unprecedented challenges for the communities and economies worldwide, causing a profound effect on the lives of billions of people. While government and communities are trying to contain the spread, researchers are aggressively focusing their efforts towards finding a treatment and/or vaccine. Science is more important now than ever before. This webinar will feature the current analytical technologies and solutions that Shimadzu is committed to provide – such as analytical techniques for drug testing and vaccine research, production and testing of sanitizers and personal protective equipment and analysis of medical sewage waste.

There is a shifting trend in the analytical platform from immunoassay to mass spectrometry in clinical research and diagnostic lab in the past decade. This growing trend of mass spectrometry application in clinical lab is due to the superior selectivity and sensitivity of mass spectrometers. More importantly, mass spectrometry can analyse multiple compounds in a single analysis using much lesser sample amount compared to immunoassay. The application of mass spectrometry in various clinical analysis using Shimadzu’s Ultra Fast Mass Spectrometry (UFMS) Technology will be discussed in this webinar. Lastly, Shimadzu latest innovative technology in clinical automation and rapid screening will also be highlighted.

This exclusive Shimadzu Food Safety Webinar will provide an in-depth know-how about the various mass spectrometry (MS/MS) workflows that are needed to conduct high throughput pesticide analysis in challenging matrices. You will learn about the latest state-of-the-art technologies that mass spec leaders are using globally to deal with pesticide analysis in difficult matrices such as spices and tobacco. This webinar will be useful for anyone who is keen to learn about the latest advanced MS/MS technologies and will be particularly interesting for those who have faced difficulties while using challenging matrices.

The increasing complexity of the food supply chain, the exponential growth of chemical hazards and their regulations have brought several challenges to the field of food safety. The need for productive gains and increased confidence in reporting non-compliant samples are particularly noteworthy challenges for laboratories that are routinely relied upon for maintaining a safe food supply chain. To meet these demands, mass spectrometric techniques such as tandem mass spectrometry and quadruple-time-of-flight mass spectrometry have emerged as the detectors of choice for the analysis of organic chemical hazards in foods and beverages.

One of the more recent significant developments is Shimadzu’s ultra fast family of mass spectrometric detectors that provide opportunities to increase both productivity and reporting confidence compared with more conventional mass spectrometric detectors. The importance of these state-of-the-art instruments to the analysis of chemical hazards in food will be discussed in the context of the regulatory requirements for the analysis of contaminants in foods and a general overview of chemical hazards in foods.

In this webinar, our panel of experts will discuss the market for alcohol-based hand sanitizers, the current regulatory environment, as well as analytical solutions including gas chromatography, Fourier-transform infrared spectroscopy, and UV-Vis spectroscopy to ensure the purity, potency, and safety of alcohols and hand sanitizers.

The properties and structures of bioplastics and biodegradable plastics are very different from regular plastics. Material identification is important for proper handling and separation. In this webinar, we go over some interesting examples of materials made of biodegradable and recycled plastics, along with technical details on their identification and differentiation.

Oftentimes, the infrared libraries consist of spectral information of pure substance in their ideal states. This webinar covers details on how to approach detection and measurement of degraded particles. In addition, technical details on how to prepare these sample, carry out measurement and work with libraries, along with some examples of real-world samples, are discussed.

Simple good practices for quality data including: Sample prep and key accessories, Attenuated Reflectance Accessory (ATR), Environmental effects - CO₂ and H₂O, Library search and precautions, and Micro analysis

This webcast will consist of two parts. Part 1 will start off with a basic introduction to luminescence terminology and electronic theory. Particular attention will be given to photoluminescence, both fluorescence and phosphorescence. Part 2 will discuss several important materials characterization applications.

This webinar will consist entirely of spectra from a series of example samples that cover the range of specular and diffuse reflectance/transmission characteristics. We will concentrate on the decision-making process of how to measure these different sample types with both the stand-alone instrument and integrating sphere accessary. Included will be a detailed discussion of how to evaluate spectral data from the various sphere measurement formats. This webinar will also cover all the basic measurement configurations of integrating spheres and how to properly evaluate the sample’s material properties from collected spectra.

We are interested in the growth of new materials and investigate physical properties affected by size, chemical doping, interface, heterostructure, and external tuning parameters (light, magnetic field, etc.). We are particularly interested in understanding the optical and dielectric properties of novel electronic materials that are fabricated in our lab in thin film form. Since the discovery of graphene, Transition metal dichalcogenides (TMDs) have merged as one of the most exciting systems to investigate properties at the nanoscale. In our lab, we have developed few-layer large-area MoS₂ thin-films using a PVD-CVD growth process that can produce films with tunable properties. For this talk, I will specifically focus on their broadband dielectric properties measured using Shimadzu UV3600 Plus. Next, I will discuss our work on the influence finite-size in Topological insulator Bi₂Se₃ thin-films. Through a combination of quantum confinement and Burstein-Moss effect, we discovered a large optical blue-shift in Bi₂Se₃ as it approaches the 2D limit.

This webcast will consist of two parts. Part 1 will start off with a basic introduction to UV/Vis/NIR integrating spheres. We will then consider samples, such as plastic films and colored Spectralon®, that illustrate how sphere diameter can impact spectral accuracy in both reflectance and scatter transmission measurements. Part 2 will discuss two novel integrating sphere applications within the rapidly growing technology field of nanomaterials.

Complying with the European Union (EU) or India's directive on Restriction of Hazardous Substances (RoHS) is an ongoing challenge for many companies. Too often, their compliance activities are unnecessarily complicated by a lack of understanding about what the regulation requires, and how to stay compliant when new restricted substances are introduced. Here in this webinar, our expert team will try to explain about the latest regulation, its implications, along with easy testing solutions to stay compliant to avoid any enforcement action.

In this webinar, our panel of experts will discuss the market for alcohol-based hand sanitizers, the current regulatory environment, as well as analytical solutions including gas chromatography, Fourier-transform infrared spectroscopy, and UV-Vis spectroscopy to ensure the purity, potency, and safety of alcohols and hand sanitizers.

Instrumental parameters that are crucial to measuring materials characterization samples are stray light, noise, resolution, and scan speed. In this presentation we will address how these inter-related parameters influence measurement success. It is vital that the proper accessory be employed to match the measurement desired. This presentation will address the function of integrating spheres and how they can be used to measure both diffuse reflection and transmission. Also discussed will be the difference between a relative and absolute reflection measurement. Finally, an overview will be given of important accessories related to the materials characterization marketplace.

There is currently a great deal of regulatory focus on data integrity, especially in the QC Laboratory. This is causing pharmaceutical (and other) companies to re-examine their practices and processes and their use of analytical instruments and data management systems in order to not only assure data integrity, but to do so in a way that is capable of demonstrating compliance with international regulatory requirements. This webinar will present on the topic of laboratory data integrity, examining this from the perspective of Laboratory Management, Supervisors and Technicians.

This presentation will cover: Timeline of Data Reliability Regulations, Guideline for Computerized Systems, Regulatory Notices and Warnings Regarding Quality Testing, Other Points on Data Integrity

As the world grapples with COVID-19 pandemic and plans for the aftermath, digitalization of laboratory workspace has become more urgent than ever before. In the future, most scientists will be telecommuting - away from the laboratory – for data analysis and reporting. Time spent in the laboratory itself will be solely for the purpose of analysing samples and pushing data into a secure database to be retrieved remotely. In this 45-min webinar, we will be discussing Shimadzu’s Informatics solutions – how different workflow solutions will facilitate the digitalization of your laboratory workspace by connectng your laboratory instruments, saving the data as well as allowing remote access and control for the users.

Shimadzu proudly presents a webinar highlighting several Material Science instruments ideal for USP Methods and Data Integrity Challenges: Thermal Analysis Instruments for USP Methods <891>: Differential Scanning Calorimetry (DSC) and Thermogravimetric Analysis (TGA) are ideal for purity, solvent determinations, polymorphism studies, and quantification analysis. In addition, USP 661.1, 661.2 for Plastics Packaging. Shimadzu Laser Diffraction Particle Size Analyzers: USP <429> particle sizing for the pharmaceutical industry EDX-7000 X-ray Fluorescence Spectrometer for USP <735>, USP <232> for Elemental Impurities Analysis. EZ-X Universal Tester to determine the compression strength of pills/tablets, press-through-packaging evaluation, syringe needle-injectability tests, syringe extrusion testing, and peel strength of adhesive bandages.

Recently, there is an increased demand for surface observation & analysis of elemental distribution on sample surfaces, particularly for metals where it can be used for failure analyses. Being the most effective non-destructive technique for qualitative and quantitative elemental analysis, Shimadzu would like to introduce the Electron Probe Micro-Analyzer (EPMA) as a precise & accurate technique for surface characterization.

Over the past few decades, the widespread utility and applicability of X-ray photoelectron spectroscopy (XPS) in research and development has made it the most popular and widely used technique of surface analysis. Our latest generation spectrometer, the AXIS Supra+, combines market leading spectroscopic and imaging capabilities with automation to ensure high sample throughput and ease of use. State-of-the-art performance including fast, high spatial resolution XPS imaging reveals the lateral distribution of surface chemistry and aids further characterisation with selected area analysis. We demonstrate the capabilities of the AXIS Supra+ and the technique of XPS in answering materials characterisation questions in application areas such as thin-films, biomaterials and Li-ion batteries.

ASTM D638 and ASTM D790 are two of the most common test methods for the evaluation of plastic tensile and flexural properties. This webinar will provide an overview of the main factors to keep in mind when performing these tests to make sure you are conforming to the standards and getting good results. It will also discuss some similarities and differences with corresponding ISO methods, ISO 527 and 178, and discuss important instrument requirements such as extensometer accuracy and software capabilities.