Nexera-e Comprehensive Two-Dimensional LC System

Comprehensive Two-Dimensional Liquid Chromatograph

The comprehensive 2D-LC methodology is a paradigm shift in liquid chromatography separation analysis. By combining two independent separation modes orthogonally in combination with a dual-loop/dual-valve alternate switching design, the highest possible peak capacity is achieved for LC chromatography separation. The new Nexera-e enables separation of even the most complex mixtures, providing a new level of knowledge and understanding of sample analytes. This is especially beneficial to the analysis of pharmaceutical impurities, proteolytic digests, food extracts, natural products and synthetic polymers.

Separation far exceeding conventional 1D-LC

How many peaks are co-eluted under one peak? The more complex the sample, along with a high percentage of similar compounds, means there is a high probability of co-eluting compounds found under single peaks.
Even though the co-eluting compounds may be unable to be separated by mono-dimensional separation, the orthogonal two-dimensional system will provide the best possible result.

Comparison of the separation of a traditional Chinese medicine sample using Nexera-e and
a conventional 1D-LC system

As shown, the figures the unseparated compounds with the mono-dimensional separation system can be separated with the two-dimensional system. Moreover, in the same analysis time, Nexera-e shows more than 200 peaks versus only 74 peaks in the conventional 1D-LC system.

* Appearance and specifications are subject to change without notice.

Features

Maximizing the full potential of two-orthogonal separation systems to perform comprehensive two-dimensional separation

Comprehensive 2D-LC is a new analytical methodology combining two independent separation modes orthogonally, greatly increasing separation efficiency.
Analysis of samples on a two-dimensional map

On a two-dimensional map, since each 1st-dimensional axis and 2nd-dimensional axis are set according to different separation conditions, the physical properties of compounds and their position on a map correlate.
Adoption of Nexera X2 components offering ultimate performance

Comprehensive 2D-LC requires excellent performance from each component. For example, the 2nd-dimensional separation system requires ultra-first gradient analysis with a high flow rate and ultra-high pressure.
2D Qualitative & Quantitative analysis using Contour Graphics

Acquired data is converted to two-dimensional contour plotting using ChromSquare, the software for comprehensive 2D-LC analysis.

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Videos

Nexera-e (Comprehensive Two-Dimensional Liquid Chromatograph)

Liquid Chromatography

Gas Chromatograph-Mass Spectrometry
Comprehensive 2DLC: Concepts, Potential, Applications

Liquid Chromatography

Gas Chromatograph-Mass Spectrometry

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