How to: Prime and Purge an i-Series HPLC Pump
Many of the most common questions about operating a Shimadzu i-series HPLC system can be addressed by using the Autopurge function prior to making the first injection of the day. This video shows how to prepare the HPLC system for purging all mobile phase lines as well as the autosampler line. When these steps are followed and the Autopurge function is finished, a constant flow and pressure can be achieved, which makes the chromatogram collected reproducible every day!