PPSQ-51A/53A

Protein Sequencer

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The PPSQ is an instrument for determining the amino acid sequences of proteins and peptides, which combines an Edman reaction section with a high performance liquid chromatograph (HPLC). There are 2 types: the PPSQ-51A, which is equipped with one reactor, and the PPSQ-53A, which is equipped with three reactors. On the PPSQ-53A, the continuous analysis of the amino acid sequences of multiple samples can be performed one after another. In the Edman reaction section, amino acids are cleaved in order from the N-terminal of a protein by repeatedly performing Edman degradation, and are derivatized. As a result, stable PTH-amino acids are produced. The PTH-amino acids are injected online into the HPLC, and analysis is performed. The HPLC data is saved on the PC, and data processing software is used to process the chromatograms. Then, amino acid sequence estimation software is used to identify the amino acids and estimate the sequences.

Isocratic System

Isocratic System

Gradient System

Gradient System

Benefits of using Edman degradation for amino acid sequencing:

(1) Guaranteed N-terminal sequence of proteins
(2) No sample pre-treatment required
(3) Differentiation of isobaric amino acids (leucine and isoleucine)
(4) Identification possible even from unknown proteins not registered in databases

Features

  • Compliance with FDA 21 CFR Part 11
    Compliance with FDA 21 CFR Part 11
    LabSolutions PPSQ software provides compliance with FDA 21 CFR Part 11 guidelines and enables compliance with the security, user management, and audit trail requirements specified by FDA 21 CFR Part 11....
  • Isocratic System
    Isocratic System
    Baseline Stability
    PPSQ series protein sequencers separate PTH-amino acids isocratically. This improves baseline stability and allows high ...
  • Gradient System
    Gradient System
    High-Sensitivity Analysis
    A high-sensitivity flow cell enables high-sensitivity detection of PTH-amino acids, which allows for sequential analysis using trace samples...
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Videos

  • Calibrating and Automated Edman Degradation System (PPSQ)

    • Protein Sequencer

    This video demonstrates how to load, run and calibrate an Automated Edman Degradation (PPSQ) system using a PTH-Amino Acid standard.

  • Running Apomyoglobin on an Automated Edman Degradation System (PPSQ)

    • Protein Sequencer

    This video demonstrates how to run Apomyoglobin, our protein standard, on our Automated Edman Degradation (PPSQ) system.

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