LC/MS/MS Method Package for Lipid Mediators - Features

For LabSolutions™ LCMS Software

Ready-to-Use Analytical Conditions

Lipid mediators (bioactive lipids) have important physiological functions and have been associated with allergies, thrombosis and lifestyle-related diseases. This method package provides a simultaneous analysis method that encompasses totally 214 compounds, which include 196 compounds of lipid mediators derived from arachidonic acid cascade and 18 internal standard compounds.

Ready-to-Use Analytical Conditions

 

Retention Time Correcting Tool Supports Identification of Isomers

The Retention Time Correcting Tool available in this version simplifies retention time correction, enabling precise identification of isomers that cannot be distinguished by MRM. The 196 compounds are divided into 18 groups based on their properties, and internal standard samples have been chosen for each group, making it possible to correct for quantitation errors that may arise, such as during solid phase extraction.

Retention Time Correcting Tool Supports Identification of Isomers

 

UFMS Technology Covers a Wide Range of Compound Groups

Fatty acids are usually detected with ESI-, but for a few important lipid mediators such as anandamide (AEA), ESI+ is preferred. Conventional joint analysis methods use only unipolar analysis. But the LCMS-8060 (NX) is capable of high-speed polarity reversal in just 5 ms, allowing many more types of compounds to be analyzed simultaneously. Compounds listed for joint analysis include 99 arachidonic acid derivatives, 26 EPA derivatives, 23 DHA derivatives, 11 ethanolamides, and 37 others, including other fatty acid metabolites and platelet-activating factor (PAF).

LC/MS/MS Method Package for Lipid Mediators Ver. 3

Index of compounds

 

Category Cord

 

Remarks and Precautions
LabSolutions LCMS Ver. 5.99SP2 or later and LabSolutions Insight Ver. 3.7SP3 or later are required.

 

 

LabSolutions is a trademark of Shimadzu Corporation.

 

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