iMLayer AERO - Features
Automatic Sprayer for MALDI Imaging
Imaging with High Reproducibility
Humidity Control
Humidity does not impact matrix deposition because the atmosphere within the spray chamber is replaced before pretreatment. Spraying can be performed under more stable conditions than with a hand spray.
Clog-Free Reagent Delivery
If the matrix clogs the nozzle tip, the spray becomes unstable, which can lead to lower reproducibility. The rinsing mechanism allows for clog-free, stable spraying, which enables high reproducibility in MALDI analyses.
Both Sensitivity and Spatial Resolution Achieved
Excellent Spray Performance
The standard protocol provides sensitivity on par with a hand spray along with improved resolution, homogeneity and reproducibility
Two-Step Vapor Deposition
To acquire high spatial resolution (5 to 10 μm) images and increase sensitivity, a two-step vapor deposition method can be implemented in combination with the iMLayer matrix vapor deposition system has been developed.* This unique experiment can only be implemented using Shimadzu sample preparation solutions.
* Patent No.: JP6153139 and JP6183779
Two-Step Vapor Deposition Allows for Very Fine Images with Minimal Blur
Hand Spray
- Matrix：9-AA
- Volume used：200 μL
Two-Step Vapor Deposition
- Film thickness：1 μm
- Solution delivery volume：120 μL/min
- Stage speed：70 mm/sec
- Laminating layers：4
Applicable to Pretreatment Other than Matrix Coatings
Derivatization Treatment
The system is also compatible with derivatization treatment (Girard's reagent T).
A single iMLayer AERO unit provides extensive MSI pretreatment coverage.
Using derivatization can improve the ionization efficiency of testosterone and other substances that are difficult to ionize.