Troubleshooting Autosampler Problems

Nexera Virtual Advisor

Symptom	Cause	Solution
Leak - low pressure valve	Rotor and stator seal and worn	Replace the rotor and stator as necessary.
Leak - high pressure valve	Rotor and stator seal and worn	Replace the rotor, inspect and replace the stator as necessary.
Leak - flow line	Connections are loose or faulty	Tighten or replace the male nuts and ferrules of the flow line connections.
Excessive pressure fluctuation with movement of the high-pressure valve	The flow rate of the mobile phase is too high	Replace the SIL outlet tubing with 0.25 mm ID PEEK tubing.
Excessive pressure fluctuation with movement of the high-pressure valve	Incorrect high-pressure valve position - NO HPV HOME displayed	Contact your Shimadzu representative.
Excessive pressure fluctuation with movement of the high-pressure valve	High-pressure valve blockage	Disassemble and clean the high-pressure valve. Replace the rotor and stator as necessary.
Excessive pressure fluctuation with movement of the high-pressure valve	Flow line blockage	Clean or replace any blocked flow lines. Remove the blockage with reverse cleaning of the flow lines.
Excessive column inlet pressure	Flow line blockage	Clean or replace any blocked flow lines. Remove the blockage with reverse cleaning of the flow lines.
Excessive column inlet pressure	Column blockage	Replace the column.
Power will not turn ON	Power supply specifications have not been set	Use a power supply that meets the instrument specifications.
Power will not turn ON	Power cable is unplugged	Properly connect the power cable to the power supply.
Power will not turn ON	Power cable is cut or broken	Replace the power cable with a new one.
Power will not turn ON	Fuse is blown	Replace the fuses. If fuses blow out frequently, contact your Shimadzu representative.