Nexera HPLC Troubleshooting the UV Detector

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<div class="container"><h1 class="title">Troubleshooting the UV Detector</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-uv-detector/spd-20a-power-indicator.jpg" alt="Troubleshooting the UV Detector"></div> <h3>Examine the power indicator on the front of the detector.</h3> The power indicator bar is located on the right side of the display panel: <ul> <li>Green indicates that the power is ON.</li> <li>Red indicates that an error has been generated.</li> <li>Orange indicates that the unit is in sleep mode.</li> </ul> <div class="ts-question">Is the indicator illuminated?</div><div class="buttons"> <span class="btn-yes">[[Yes->OVER Displayed - SPD-20A/AV (1562)]]</span> <span class="btn-no">[[No->Power Connections - SPD (1502)]]</span> </div> </div>

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<div class="container"><h1 class="title">OVER Displayed - SPD-20A/AV</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-uv-detector/over.jpg" alt="OVER Displayed - SPD-20A/AV"></div> When the absorbance reading is above or below the limits of the detector, OVER will be displayed for the absorbance value. This may also happen if the baseline position has not been zeroed. <div class="ts-question">Is OVER displayed as the absorbance value?</div><div class="buttons"> <span class="btn-yes">[[Yes->ZERO Key (1565 / 1628)]]</span> <span class="btn-no">[[No->Baseline Change (1567)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Power Connections - SPD</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-uv-detector/power-spd.jpg" alt="Power Connections - SPD"></div> <h3>Examine the connections of the power supply cord.</h3> One end of the power cable is inserted into the power connector on the back of the detector. The other end of the power cable is inserted into the power supply outlet. <img class="warning" style="float: left; margin-bottom:25px;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>WARNING: The three-line type power cable supplied as an accessory includes the grounding wire. Connect this wire to ground to prevent electrical shock and to secure stable operation of the instrument. CAUTION: Ensure that the power switch is in the OFF position before connecting the power cable.</strong> <div class="ts-question">Is the power cord properly connected to the detector and power supply outlet?</div><div class="buttons"> <span class="btn-yes">[[Yes->Power Cord Damage (1505)]]</span> <span class="btn-no">[[No->Making Power Connection (1504)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">ZERO Key</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-uv-detector/zero-key-spd-20.jpg" alt="ZERO Key"></div> Press the ZERO key on the front of the detector to return the baseline to the zero position. The ZERO key is located to the right of the keypad and is functional even if the instrument is in REMOTE operation mode. <img class="warning" style="float: left; margin-bottom:25px;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>NOTE: If necessary, the zero position can be adjusted using the BL OFS ITG (integrator) and BL OFS REC (recorder) screens in the Parameter Setting Group.</strong> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Baseline Change</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-uv-detector/signal-noise-spd-20.jpg" alt="Baseline Change"></div> <h3>Enlarge the baseline and examine it for an indication of signal.</h3> By enlarging the baseline and closely examining it for the presence of normal system noise, it can be determined whether the signal is actually reaching the data system. All baselines have an inherent amount of normal system noise. If there is no system noise, it is an indication that the signal is not reaching the data system. If system noise is present, it is an indication that the detector is at least responding minimally, but the injection process or detector may not be working properly. Enlarge the baseline and examine it for an indication of signal. <div class="ts-question">Does the baseline show any signs of change?</div><div class="buttons"> <span class="btn-yes">[[Yes->Flow Cell Temperature Inspection - SPD-20A/AV (1630)]]</span> <span class="btn-no">[[No->AUX RANGE Parameter (1579)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Power Cord Damage</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-uv-detector/power-cord-damage.jpg" alt="Power Cord Damage"></div> Handle the power cord with care. Observe the following precautions to avoid the possibility of cord damage that may result in fire, electric shock or instrument malfunction. <ul> <li>Do not place heavy objects on the cord.</li> <li>Keep hot items away from the power cord.</li> <li>Do not modify the power cord in any way.</li> <li>Do not bend the cord excessively.</li> <li>To unplug the cord, pull on the plug itself, NOT on the cord.</li> <li>If the cord becomes damaged, replace it with a new one immediately.</li> </ul> <div class="ts-question">Is the power cord damaged?</div><div class="buttons"> <span class="btn-yes">[[Yes->Power Cord Replacement (1506)]]</span> <span class="btn-no">[[No->Power Supply Requirements- SPD-20 (1523)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Making the Power Connection</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-uv-detector/power-cto.jpg" alt="Making the Power Connection"></div> <ol> <li>Turn the power switch on the detector OFF before plugging the instrument into the power supply.</li> <li>Insert the plug end of the power cable directly into the power supply outlet.</li> <li>Insert the connector end of the power cable into the power connector on the back of the detector.</li> </ol> <img class="warning" style="float: left; margin-bottom:25px;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>WARNING: The three-line type power cable supplied as an accessory includes the grounding wire. Connect this wire to ground to prevent electrical shock and to secure stable operation of the instrument.</strong> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Power Cord Replacement</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-uv-detector/power-cord.jpg" alt="Power Cord Replacement"></div> A power supply cable is supplied with each detector. If the cord becomes damaged, replace it with a new one immediately. Visit <a href="https://store.shimadzu.com" target="_blank">our webstore</a> to order parts. <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Power Supply Requirements- SPD-20</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-uv-detector/spd-20a-av-power-req.jpg" alt="Power Supply Requirements- SPD-20"></div> Verify that the power outlet has a sufficient capacity. If capacity is insufficient, a power outage or voltage drop can occur. Power drops will affect the performance of this instrument and any other instruments that are connected to the same power supply circuit. <div class="ts-question">Does the power supply meet the power supply requirements?</div><div class="buttons"> <span class="btn-yes">[[Yes->Checking the Fuses - SPD-20A/AV (1541)]]</span> <span class="btn-no">[[No->Power Supply Requirements - SPD-20A/AV (1533)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Checking the Fuses - SPD-20A/AV</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-uv-detector/fuses-spd.jpg" alt="Checking the Fuses - SPD-20A/AV"></div> <h3>If the circuit has been overloaded, the fuses may blow to protect the system electronics.</h3> <ol> <li>Open the fuse compartment cover with a screwdriver.</li> <li>Remove both of the fuse holders from the fuse compartment.</li> <li>Remove the old fuses from the fuse holders.</li> <li>Inspect the fuse by holding it up to the light. The glass should be clear and clean and the wire should be continuous and extend from one end of the fuse to the other. The fuse is blown and must be replaced if the glass is discolored with black or brown smudges or the wire is clearly broken or not visible at all.</li> <li>Insert the new fuses into the fuse holders.</li> <li>Reinstall the fuse holders.</li> </ol> <img class="warning" style="float: left; margin-bottom:25px;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>WARNING: Turn the instrument OFF and disconnect the power supply before proceeding.</strong> <div class="ts-question">Have the fuses blown?</div><div class="buttons"> <span class="btn-yes">[[Yes->Fuse Replacement (1109)]]</span> <span class="btn-no"><a href="https://www.ssi.shimadzu.com/forms/tech-support/index.html?product_category=VirtualAdvisor&product_name=Nexera&page=webform_start">Contact Shimadzu</a></span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Power Supply Requirements - SPD-20A/AV</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-uv-detector/spd-20a-av-power-req.jpg" alt="Power Supply Requirements - SPD-20A/AV"></div> Ensure that the power supply meets the power supply requirements. If the power supply does not meet the specifications the instrument may not function properly or become damaged. If the power supply does not meet specifications, take corrective action before connecting this unit to the power circuit. <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Fuse Replacement</h1> <div class="center"><iframe width="900" height="506" src="https://www.youtube.com/embed/b7YnT0wHIRE?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>If the circuit has been overloaded, the fuses may blow to protect the system electronics.</h3> <img class="warning" style="float: left; margin-bottom:25px;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>WARNING: Turn the instrument OFF and disconnect the power supply before proceeding.</strong> <ol> <li>Open the fuse compartment cover with a screwdriver.</li> <li>Remove both of the fuse holders from the fuse compartment.</li> <li>Remove the old fuses from the fuse holders.</li> <li>Insert the new fuses into the fuse holders.</li> <li>Reinstall the fuse holders.</li> <li>Close the fuse compartment cover.</li> </ol> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Flow Cell Temperature Inspection - SPD-20A/AV</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-uv-detector/cell-temp-inspect.jpg" alt="Flow Cell Temperature Inspection - SPD-20A/AV"></div> The detector has the ability to control the flow cell temperature. External temperature fluctuation affects the stability of the baseline. This affect is minimized by controlling the flow cell temperature. Temperature control of the flow cell is particularly effective when the mobile phase is highly absorptive or the sample absorbance is temperature dependent. <span class="btn-link">[[Temperature Inspection->Flow Cell Temperature Inspection - SPD-20A/AV (10506)]]</span> <div class="ts-question">Is the temperature of the flow cell steady?</div><div class="buttons"> <span class="btn-yes">[[Yes->Lamp Energy (2262)]]</span> <span class="btn-no">[[No->Flow Cell Heater Cable - SPD-20A/AV (1632)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">AUX RANGE Parameter</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-uv-detector/aux-range.jpg" alt="AUX RANGE Parameter"></div> The AUX RANGE setting affects both the analog and digital output signal from the SPD-20A and SPD-20AV UV detectors. The table at the left displays the AUX RANGE settings that area possible with the SPD-20A and SPD-20AV detectors. Set the AUX RANGE to 4 (4.0 AU/V) to determine whether the flat-topped peaks are related to saturated optics. If the peaks continue to be flat-topped at this setting, the sample is too concentrated for the limitations of the optics of the detector. The sample concentration or injection volume must be decreased. <div class="ts-question">Is the RANGE set to 0?</div><div class="buttons"> <span class="btn-yes">[[Yes->Setting the AUX RANGE Parameter (10471)]]</span> <span class="btn-no">[[No->LAMP On (1624)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Lamp Energy</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-uv-detector/lamp-on-off.jpg" alt="Lamp Energy"></div> The lamps are consumable and eventually become too weak to be effective. Click the appropriate lamp/detector link to learn how to check the reference intensities for that detector. <h3>Acceptable Limits</h3> <strong>SPD-20A</strong> D2 - ref intensity at 220nm > 400 <strong>SPD-20AV</strong> D2 - ref intensity at 220nm > 400 W - ref intensity at 540nm > 500 <strong>SPD-M20A</strong> D2 - maximum intensity at or around 235nm >7V W - maximum intensity at or around 550-600nm >7V <div class="ts-question">Are the lamp reference intensities adequate?</div><div class="buttons"> <span class="btn-yes">[[Yes->Deuterium Lamp Replacement - SPD-20A (1107)]]</span> <span class="btn-no">[[No->Baseline Spikes - SPD-20A/AV (1711)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Flow Cell Heater Cable - SPD-20A/AV</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-uv-detector/spd-20a-av-flow-cell-heater-cable.jpg" alt="Flow Cell Heater Cable - SPD-20A/AV"></div> The temperature control function allows for control of the flow cell temperature. In order for this function to operate, the flow cell heater must be connected to a power source. Examine the cable of the flow cell heater and ensure that it is properly connected to the detector. <div class="ts-question">Is the cable for the flow cell heater properly connected?</div><div class="buttons"> <span class="btn-yes">[[Yes->Flow Cell Temperature Setting - SPD-20A/AV (1634)]]</span> <span class="btn-no">[[No->Flow Cell Heater Cable Procedure- SPD-20A/AV (1633)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Flow Cell Temperature Inspection - SPD-20A/AV</h1> <ol> <li>From the Initial screen, press FUNC one time to display the Parameter screen.</li> <li>Press ENTER.</li> <li>The LAMBDA 1 screen is displayed.</li> <li>Press the FUNC key until the CELL TEMP screen is displayed.</li> <li>Monitor the flow cell temperature and verify that it is stable.</li> </ol> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Setting the AUX RANGE Parameter</h1> <div class="center"><iframe width="900" height="506" src="https://www.youtube.com/embed/kPgR6UMtDqg?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <ol> <li>From the Initial screen, press the FUNC key. The PARAMETER screen is displayed.</li> <li>Press the ENTER key and then press the FUNC key four times. The AUX RANGE parameter screen is displayed.</li> <li>Enter the numeric value (1-6) for the parameter and press the ENTER key.</li> <li>Press the CE key twice to return to the Initial screen.</li> </ol> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">LAMP On</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-uv-detector/lamp-on-off.jpg" alt="LAMP On"></div> <h3>Examine the lamp power screen. Press the LAMP Power button to review the procedure for checking the LAMP Power setting.</h3> <span class="btn-link">[[LAMP Power->LAMP Check - SPD-20AV (10505)]]</span> <div class="ts-question">Has the appropriate lamp power been turned ON?</div><div class="buttons"> <span class="btn-yes">[[Yes->Over is Displayed (1627)]]</span> <span class="btn-no">[[No->Turning the LAMP Power ON and OFF - SPD-20A and AV (10483)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">LAMP Check - SPD-20AV</h1> <ul> <li>0 - Turns all lamps OFF</li> <li>1 - Turns the Deuterium lamp ON</li> <li>2 - Turns the Tungsten lamp ON (SPD-20AV only)</li> </ul> <ol> <li>Turn the instrument power switch ON. The Initial screen is displayed.</li> <li>Press the FUNC key. The PARAMETER screen is displayed.</li> <li>Press the FUNC key. The PARAMETER screen is displayed.</li> <li>Press the FUNC key three times. The LAMP screen is displayed.</li> </ol> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Over is Displayed</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-uv-detector/over.jpg" alt="Over is Displayed"></div> When the absorbance reading is above or below the limits of the detector, OVER will be displayed for the absorbance value. This may also happen if the baseline position has not been zeroed. <div class="ts-question">Has the ZERO button been pressed?</div> <div class="buttons"> <span class="btn-yes"><a href="https://www.ssi.shimadzu.com/forms/tech-support/index.html?product_category=VirtualAdvisor&product_name=Nexera&page=webform_start">Contact Shimadzu</a></span> <span class="btn-no">[[No->ZERO Key (1565 / 1628)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Turning the LAMP Power ON and OFF - SPD-20A and AV</h1> <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>NOTE: Lamp Settings:</strong> 0 - Turns all lamps OFF 1 - Turns the Deuterium lamp ON 2 - Turns the Tungsten lamp ON (SPD-20AV only) 3 - Turns both the Deuterium and Tungsten lamps ON (SPD-20AV only) <strong>Steps</strong> <ol> <li>Turn the instrument power switch ON. The Initial screen is displayed.</li> <li>Press the FUNC key. The PARAMETER screen is displayed.</li> <li>Press ENTER. The Channel 1 wavelength-setting screen (LAMBDA 1) is displayed.</li> <li>Press the FUNC key three times. The LAMP screen is displayed.</li> <li>Select a value from below and enter using the numeric keypad.</li> </ol> <span class="btn-link">[[SPD-M20A->Turning the LAMP Power ON and OFF - SPD-M20A (1255)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Turning the LAMP Power ON and OFF - SPD-M20A</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-uv-detector/lamp-on-off.jpg" alt="Turning the LAMP Power ON and OFF - SPD-M20A"></div> <ol> <li>Turn ON the PC that controls the instrument and launch the web browser on the PC.</li> <li>With the instrument running, enter the IP address of the instrument in the 'Address' field. When a connection with the instrument is established, the Login screen is displayed.</li> <li>Log in to the system as a 'guest'. The Analysis Parameters screen is displayed. The current operating conditions are displayed. They can be changed or edited as needed.</li> <li>Press the UPDATE button. The parameters are updated and the Analysis Setting and Execution screen is displayed. The parameters are saved in the unit's EEPROM and are kept even after the unit is turned OFF.</li> </ol> <img class="warning" style="float: left; margin-bottom:25px;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>NOTE: When parameters are changed via the system controller or data system, they are updated accordingly.</strong> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

Combined ZERO Key https://nexerava.ssi.shimadzu.com/node/1628 https://nexerava.ssi.shimadzu.com/node/1565

<div class="container"><h1 class="title">Deuterium Lamp Replacement - SPD-20A</h1> <div class="center"><iframe width="900" height="506" src="https://www.youtube.com/embed/QcHoIfDTAms?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <ol> <li>Turn OFF the power to the instrument and unplug the power cable.</li> <li>Remove the top cover.</li> <li>Remove the radiating fin.</li> <li>Disconnect the 3-pin deuterium lamp connector.</li> <li>Loosen the screws (2) and remove the deuterium lamp.</li> <li>Install the new deuterium lamp.</li> <li>Reconnect the 3-pin deuterium lamp connector.</li> <li>Reinstall the radiating fin.</li> <li>Reinstall the top cover.</li> <li>Reset the lamp usage time [D2 LAMP USED].</li> </ol> <span class="btn-link">[[D2 Lamp Replacement - SPD-20AV->D2 Lamp Replacement - SPD-20AV (1124)]]</span> <span class="btn-link">[[D2 Lamp Replacement - SPD-M20A->D2 Lamp Replacement - SPD-M20A (1141) ]]</span> <span class="btn-link">[[W Lamp Replacement - SPD-20AV->W Lamp Replacement - SPD-20AV (1126)]]</span> <span class="btn-link">[[W Lamp Replacement - SPD-M20A->W Lamp Replacement - SPD-M20A (1143)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Baseline Spikes - SPD-20A/AV</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-uv-detector/spikes.jpg" alt="Baseline Spikes - SPD-20A/AV"></div> Generally, the more dissolved air that is removed from the mobile phase the better the entire HPLC system will perform. However, when mobile phase does contain dissolved gas, the gas can escape from solution to form a small bubble in the flow line. These small bubbles can attach to any dirt particles that may be in the flow cell. As more gas escapes from solution, the bubble becomes larger causing a disturbance to the baseline. When the bubble gets large enough to flow out with the mobile phase, the baseline returns to zero. This phenomenon usually causes a very regular sinusoidal (sawtooth) shape to the baseline. It is also possible that the bubbles will manifest themselves as spiking vertical lines in the baseline. <div class="ts-question">Are spikes present in the baseline?</div><div class="buttons"> <span class="btn-yes">[[Yes->Back Pressure Device (1716)]]</span> <span class="btn-no">[[No->Room Temperature (1720)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Back Pressure Device</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-uv-detector/back-pressure.jpg" alt="Back Pressure Device"></div> A 2 meter length of tubing is provided with the detector.This tubing functions to direct the detector waste to the waste container while it applies back pressure to the flow cell. Do not shorten the tubing on the cell outlet side. Back pressure to the flow cell outlet prevents the generation of air bubbles. Cutting the tubing to less than 2 m will interfere with the ability to provide the necessary amount of back pressure. <div class="ts-question">Is the back-pressure device connected to the pump outlet?</div><div class="buttons"> <span class="btn-yes">[[Yes->Mobile Phase Degassing (1717)]]</span> <span class="btn-no">[[No->Back Pressure Tubing (1713)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Room Temperature</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-uv-detector/column-temp.jpg" alt="Room Temperature"></div> Maintaining the column temperature at a constant level increases analysis reproducibility and separation performance. There are three different types of thermal insulation devices available for the LC column. <ul> <li>CTO-20A/20AC Column Oven - Uses an air-circulated thermostatic chamber to maintain the temperature of the LC column and flow lines.</li> <li>CTO-10ASvp Column Oven - Uses a heating block to control the temperature of the LC column.</li> <li>Jacket-type Devices - Uses plumbing insulation or heat tape to a lesser degree of effectiveness.</li> </ul> <div class="ts-question">Is the baseline drifting?</div><div class="buttons"> <span class="btn-yes">[[Yes->Column Insulation (1721)]]</span> <span class="btn-no">[[No->Baseline Stability (1723)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Mobile Phase Degassing</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-uv-detector/degas.jpg" alt="Mobile Phase Degassing"></div> <h3>Dissolved air in the mobile phase may be allowing air bubbles to form in the flow cell.</h3> Bubbles are caused by dissolved gases. These bubbles can be responsible for pump malfunction and baseline noise. To prevent the formation of bubbles in the pump head and flow cell, a degassing method must be included in the mobile phase preparation. Some typical methods include vacuum filtration, vacuum sonication and helium sparging. In addition to conducting degassing during mobile phase preparation, it is also recommended that an on-line degassing unit be included in the system. The Shimadzu on-line degasser (DGU-20A3/20A5) provides continuous mobile phase degassing without changing the composition of the mobile phase. Dissolved air in the mobile phase may be allowing air bubbles to form in the flow cell. <div class="ts-question">Has the mobile phase been properly degassed?</div><div class="buttons"> <span class="btn-yes"><a href="https://www.ssi.shimadzu.com/forms/tech-support/index.html?product_category=VirtualAdvisor&product_name=Nexera&page=webform_start">Contact Shimadzu</a></span> <span class="btn-no">[[No->Mobile Phase Degassing (1719)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Back Pressure Tubing</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-uv-detector/back-pressure.jpg" alt="Back Pressure Tubing"></div> Shimadzu detectors are all supplied with a 2-meter length of tubing to be used as a back-pressure device. One function of this tubing is to apply back pressure to the flow cell outlet to prevent the generation of air bubbles in the flow cell. <ol> <li>Attach a male PEEK nut to one of the ends of the supplied 2m cell outlet tubing.</li> <li>Screw the male PEEK nut attached to the tubing into the outlet coupling (union).</li> <li>Insert the other end of the tubing into the waste container so that the outlet end is pointing down but above the surface of the liquid in the container.</li> </ol> <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>CAUTION: Do not shorten the cell outlet tubing from the original 2m length.</strong> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Flow Cell Temperature Setting - SPD-20A/AV</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-uv-detector/cell-temp-inspect.jpg" alt="Flow Cell Temperature Setting - SPD-20A/AV"></div> The detector has the ability to control the flow cell temperature. External temperature fluctuation affects the stability of the baseline. This affect is minimized by controlling the flow cell temperature. Temperature control of the flow cell is particularly effective when the mobile phase is highly absorptive or the sample absorbance is temperature dependent. <div class="ts-question">Is the flow cell temperature properly set?</div> <div class="buttons"><span class="btn-yes"><a href="https://www.ssi.shimadzu.com/forms/tech-support/index.html?product_category=VirtualAdvisor&product_name=Nexera&page=webform_start">Contact Shimadzu</a></span> <span class="btn-no">[[No->Flow Cell Temperature Inspection - SPD-20A/AV (10506)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Flow Cell Heater Cable Procedure- SPD-20A/AV</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-uv-detector/spd-20a-av-flow-cell-heater-cable.jpg" alt="Flow Cell Heater Cable Procedure- SPD-20A/AV"></div> The temperature control function allows for control of the flow cell temperature. In order for this function to operate, the flow cell heater must be connected to a power source. Examine the cable of the flow cell heater and ensure that it is properly connected to the detector. <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Column Insulation</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-uv-detector/column-temp.jpg" alt="Column Insulation"></div> <h3>A column oven can be used to isolate the column and flow lines from the ambient temperature fluctuations.</h3> Maintaining the column temperature at a constant level increases analysis reproducibility and separation performance. There are three different types of thermal insulation devices available for the LC column. <ul> <li>CTO-30A and the CTO-30AS with the SIL-30AC MP Column Oven - Uses a heating block to control the temperature of the LC column.</li> <li>CTO-20A/20AC Column Oven - Uses an air-circulated thermostatic chamber to maintain the temperature of the LC column and flow lines.</li> <li>Jacket-type Devices - Uses plumbing insulation or heat tape to a lesser degree of effectiveness.</li> </ul> <div class="ts-question">Is the room temperature fluctuating?</div><div class="buttons"> <span class="btn-yes">[[Yes->Column Oven (1722)]]</span> <span class="btn-no"><a href="https://www.ssi.shimadzu.com/forms/tech-support/index.html?product_category=VirtualAdvisor&product_name=Nexera&page=webform_start">Contact Shimadzu</a></span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Baseline Stability</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-uv-detector/baseline-stability.jpg" alt="Baseline Stability"></div> Make a sample injection to calculate the signal-to-noise ratio and determine if it is acceptable for your application. All baselines have an inherent amount of noise. Baseline noise is only a problem if the amount of noise is so excessive as to interfere with integration of the analyte of interest. Data systems often have an auto scale function that can make a normal baseline appear to be very noisy. The signal to noise ratio is a measure of the Peak height with respect to the Noise height. This ratio can be increased by decreasing the noise or by increasing the peak (signal). The sensitivity of the method and the scale of the chromatogram output must be determined before determining whether or not the noise is excessive. <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>NOTE: Calculating Signal-to-Noise Ratio The signal-to-noise ratio is calculated using the peak height of the noise and the peak height of the sample peak. EXAMPLE: Noise height 2mV - Sample height 10mV - The signal-to-noise ratio is 5. Typically, a signal-to-noise ratio of 3 is required for detection, and 10 for quantitation.</strong> <div class="ts-question">Is there excessive noise in the baseline?</div><div class="buttons"> <span class="btn-yes">[[Yes->Flow Cell Contamination (1724)]]</span> <span class="btn-no"><a href="https://www.ssi.shimadzu.com/forms/tech-support/index.html?product_category=VirtualAdvisor&product_name=Nexera&page=webform_start">Contact Shimadzu</a></span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Column Oven</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-uv-detector/cto-20ac.jpg" alt="Column Oven"></div> The Shimadzu column oven (CTO-20A/20AC) is an air-circulated thermostatic chamber designed to improve retention time reproducibility and separation performance by maintaining the temperature of the LC column and flow lines. In addition to the column, the oven can also house manual injectors, solvent mixers, column switching valves and other flow line accessories. Installing these items into the column oven isolates them from the influence of ambient temperature fluctuations and allows more stable analysis.<div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Flow Cell Contamination</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-uv-detector/flow-cell.jpg" alt="Flow Cell Contamination"></div> A dirty flow cell can cause baseline drift, instability, noise and other adverse affects to the chromatography. Depending on the type of samples that are routinely tested, a maintenance plan for the flow cell should be developed and adhered to for optimal flow cell performance. <div class="ts-question">Has the inside of the flow cell been flushed?</div><div class="buttons"> <span class="btn-yes">[[Yes->Pulsation Dampener - SPD-20A/AV (1728)]]</span> <span class="btn-no">[[No->Flow Cell Cleaning - SPD-20A (1103)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Pulsation Dampener - SPD-20A/AV</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-uv-detector/pulsation-dampener.jpg" alt="Pulsation Dampener - SPD-20A/AV"></div> Pulsation dampeners are used to reduce the variation in pressure caused by the action of the pump plungers. They usually consist of a larger volume section of tubing or a chamber that absorbs the pressure fluctuations. <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>NOTE: All of the pumps in the Shimadzu LC-20A series have built in pulsation dampeners in the drain valve. Therefore, if you are using the LC-20A series pumps it is not necessary to attach a pulsation dampener.</strong> <div class="ts-question">Is a pump pulsation dampener installed on the pump?</div><div class="buttons"> <span class="btn-yes">[[Yes->Flow Cell Contamination (1730)]]</span> <span class="btn-no">[[No->Pulsation Dampener (1729)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Flow Cell Cleaning - SPD-20A</h1> <div class="center"><iframe width="900" height="506" src="https://www.youtube.com/embed/JlgxpZJw0fQ?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <ol> <li>Open the doors</li> <li>Disconnect the heater assembly connector.</li> <li>Loosen the clamp securing the flow line couplings and disconnect the flow lines.</li> <li>Loosen screws and remove the flow cell.</li> <li>With mobile phase flowing, inspect the flow cell window for air bubbles or debris.</li> <li>Disconnect the flow cell inlet.</li> <li>Attach a syringe filled with 2-propanol to the flow cell inlet.</li> <li>Flush the flow cell with 2-propanol. Repeat with mobile phase.</li> <li>Reattach the flow cell inlet and reinsert the flow cell into the detector.</li> <li>Close the doors.</li> </ol> <span class="btn-link">[[Flow Cell Cleaning - SPD-20AV->Flow Cell Cleaning - SPD-20AV (1113)]]</span> <span class="btn-link">[[Flow Cell Cleaning - SPD-M20A->Flow Cell Cleaning - SPD-M20A (1137)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Flow Cell Contamination</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-uv-detector/flow-cell.jpg" alt="Flow Cell Contamination"></div> A dirty flow cell can cause baseline drift, instability, noise and other adverse affects to the chromatography. Depending on the type of samples that are routinely tested, a maintenance plan for the flow cell should be developed and adhered to for optimal flow cell performance. <div class="ts-question">Has the flow cell been dismantled and cleaned?</div><div class="buttons"> <span class="btn-yes">[[Yes->Dual Noise Mode (1734)]]</span> <span class="btn-no">[[No->Flow Cell Disassembly - SPD-20A (1105)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Pulsation Dampener</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-uv-detector/pulsation-dampener.jpg" alt="Pulsation Dampener"></div> Pulsation dampeners are used to reduce the variation in pressure caused by the action of the pump plungers. They usually consist of a larger volume section of tubing or a chamber that absorbs the pressure fluctuations. If you are using a pump that does not have a pulsation dampener, obtain one and install it. <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>NOTE: All of the pumps in the Shimadzu LC-20A series have built in pulsation dampeners in the drain valve. Therefore, if you are using the LC-20A series pumps it is not necessary to attach a pulsation dampener.</strong> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Flow Cell Cleaning - SPD-20AV</h1> <div class="center"><iframe width="900" height="506" src="https://www.youtube.com/embed/1hfwUl_ue1c?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <ol> <li>Open the doors.</li> <li>Disconnect the heater assembly connector.</li> <li>Loosen the clamp securing the flow line couplings and disconnect the flow lines.</li> <li>Loosen screws and remove the flow cell.</li> <li>With mobile phase flowing, inspect the flow cell window for air bubbles or debris.</li> <li>Disconnect the flow cell inlet.</li> <li>Attach a syringe filled with 2-propanol to the flow cell inlet.</li> <li>Flush the flow cell with 2-propanol. Repeat with mobile phase.</li> <li>Reattach the flow cell inlet and reinsert the flow cell into the detector.</li> <li>Close the doors.</li> </ol> <span class="btn-link">[[Flow Cell Cleaning - SPD-20A->Flow Cell Cleaning - SPD-20A (1103)]]</span> <span class="btn-link">[[Flow Cell Cleaning - SPD-M20A->Flow Cell Cleaning - SPD-M20A (1137)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Flow Cell Cleaning - SPD-M20A</h1> <div class="center"><iframe width="900" height="506" src="https://www.youtube.com/embed/mXug4294D64?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <ol> <li>Open the door.</li> <li>Loosen the clamp securing the flow line couplings and disconnect the flow lines.</li> <li>Disconnect the heater assembly connector.</li> <li>Loosen screws and remove the flow cell.</li> <li>Attach a syringe filled with 2-propanol to the flow cell inlet.</li> <li>Flush the flow cell with 2-propanol. Repeat with mobile phase.</li> <li>Reinsert the flow cell and finger tighten the screws.</li> <li>Reconnect the heater assembly connector.</li> <li>Reconnect the flow lines and secure the couplings in the clamp.</li> <li>Close the door.</li> </ol> <span class="btn-link">[[Flow Cell Cleaning - SPD-20A->Flow Cell Cleaning - SPD-20A (1103)]]</span> <span class="btn-link">[[Flow Cell Cleaning - SPD-20AV->Flow Cell Cleaning - SPD-20AV (1113)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Dual Noise Mode</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-uv-detector/base-noise.jpg" alt="Dual Noise Mode"></div> The dual wavelength mode permits either (a) simultaneous recording of chromatograms for two wavelengths or (b) simultaneous recording of a chromatogram of the Ch1 wavelength and ratio chromatogram of two wavelengths on Ch2. In dual wavelength mode, data is collected by sequential scanning of the grating. Noise level in dual wavelength mode varies depending on the wavelength. <div class="ts-question">Is the noise 10 times greater in dual mode than in single mode?</div><div class="buttons"> <span class="btn-yes">[[Yes->Dual Noise (1736)]]</span> <span class="btn-no"><a href="https://www.ssi.shimadzu.com/forms/tech-support/index.html?product_category=VirtualAdvisor&product_name=Nexera&page=webform_start">Contact Shimadzu</a></span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Flow Cell Disassembly - SPD-20A</h1> <div class="center"><iframe width="900" height="506" src="https://www.youtube.com/embed/QjvkN5M9mtQ?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> Clean the inner surface of the cell with a cotton swab moistened with 2-propanol. Sonicate the lenses for 5 minutes in 2-propanol. The convex lens should be on the notch side with the convex side facing outward. <ol> <li>Open the doors.</li> <li>Disconnect the heater assembly connector.</li> <li>Loosen the clamp securing the flow line couplings and disconnect the flow lines.</li> <li>Loosen screws and remove the flow cell.</li> <li>Remove the cell window screws, flat lens/convex lens and gaskets.</li> <li>Install new gaskets and flat lens/convex lens, and secure with the cell window screws.</li> <li>Check the flow cell for leaks.</li> <li>Reinsert the flow cell and finger tighten the screws.</li> <li>Reconnect the flow lines and secure the couplings in the clamp.</li> <li>Reconnect the heater assembly connector.</li> <li>Close the doors.</li> </ol> <span class="btn-link">[[Flow Cell Disassembly - SPD-20AV->Flow Cell Disassembly - SPD-20AV (1116)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Flow Cell Disassembly - SPD-20AV</h1> <div class="center"><iframe width="900" height="506" src="https://www.youtube.com/embed/7JDUwrzclQE?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> Clean the inner surface of the cell with a cotton swab moistened with 2-propanol. Sonicate the lenses for 5 minutes in 2-propanol. The convex lens should be on the notch side with the convex side facing outward. <ol> <li>Open the doors.</li> <li>Disconnect the heater assembly connector.</li> <li>Loosen the clamp securing the flow line couplings and disconnect the flow lines.</li> <li>Loosen screws and remove the flow cell.</li> <li>Remove the cell window screws, flat lens/convex lens and gaskets.</li> <li>Install new gaskets and flat lens/convex lens, and secure with the cell window screws.</li> <li>Check the flow cell for leaks.</li> <li>Reinsert the flow cell and finger tighten the screws.</li> <li>Reconnect the flow lines and secure the couplings in the clamp.</li> <li>Reconnect the heater assembly connector.</li> <li>Close the doors.</li> </ol> <span class="btn-link">[[Flow Cell Disassembly - SPD-20A->Flow Cell Disassembly - SPD-20A (1105)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Dual Noise</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-uv-detector/base-noise.jpg" alt="Dual Noise"></div> The dual wavelength mode permits either (a) simultaneous recording of chromatograms for two wavelengths or (b) simultaneous recording of a chromatogram of the Ch1 wavelength and ratio chromatogram of two wavelengths on Ch2. In dual wavelength mode, data is collected by sequential scanning of the grating. Noise level in dual wavelength mode varies depending on the wavelength. It may be necessary to: <ul> <li>Use less sensitive detector settings (higher Range values)</li> <li>Use solvents with higher purity</li> <li>Use a different wavelength</li> </ul> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Deuterium Lamp Replacement - SPD-20AV</h1> <div class="center"><iframe width="900" height="506" src="https://www.youtube.com/embed/Ea9Ho1yybTo?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <ol> <li>Turn OFF the power to the instrument and unplug the power cable.</li> <li>Remove the top cover.</li> <li>Remove the radiating fin.</li> <li>Disconnect the 3-pin deuterium lamp connector.</li> <li>Loosen the screws (2) and remove the deuterium lamp.</li> <li>Install the new deuterium lamp.</li> <li>Reconnect the 3-pin deuterium lamp connector.</li> <li>Reinstall the radiating fin.</li> <li>Reinstall the top cover.</li> <li>Reset the lamp usage time [D2 LAMP USED].</li> </ol> <span class="btn-link">[[D2 Lamp Replacement - SPD-20A->Deuterium Lamp Replacement - SPD-20A (1107)]]</span> <span class="btn-link">[[D2 Lamp Replacement - SPD-M20A->D2 Lamp Replacement - SPD-M20A (1141) ]]</span> <span class="btn-link">[[W Lamp Replacement - SPD-20AV->W Lamp Replacement - SPD-20AV (1126)]]</span> <span class="btn-link">[[W Lamp Replacement - SPD-M20A->W Lamp Replacement - SPD-M20A (1143)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Deuterium Lamp Replacement - SPD-M20A</h1> <div class="center"><iframe width="900" height="506" src="https://www.youtube.com/embed/ASOXCGd5P4g?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <ol> <li>Turn OFF the power to the instrument and unplug the power cable.</li> <li>Open the doors.</li> <li>Remove the lamp cover.</li> <li>Disconnect the 3-pin deuterium lamp connector.</li> <li>Remove the lamp and lamp cap from the instrument.</li> <li>Remove the lamp cap from the old lamp and place it on the new lamp in the correct orientation.</li> <li>Reinstall the D2 lamp assembly.</li> <li>Reconnect the 3-pin connector.</li> <li>Reinstall the lamp cover.</li> <li>Close the doors.</li> <li>Reset the lamp usage time to 0, and perform the exposure time setting.</li> </ol> <span class="btn-link">[[D2 Lamp Replacement - SPD-20A->Deuterium Lamp Replacement - SPD-20A (1107)]]</span> <span class="btn-link">[[D2 Lamp Replacement - SPD-20AV->D2 Lamp Replacement - SPD-20AV (1124)]]</span> <span class="btn-link">[[W Lamp Replacement - SPD-20AV->W Lamp Replacement - SPD-20AV (1126)]]</span> <span class="btn-link">[[W Lamp Replacement - SPD-M20A->W Lamp Replacement - SPD-M20A (1143)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Tungsten Lamp Replacement - SPD-20AV</h1> <div class="center"><iframe width="900" height="506" src="https://www.youtube.com/embed/mhYWiItgUqA?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <ol> <li>Turn OFF the power to the instrument and unplug the power cable.</li> <li>Remove the top cover.</li> <li>Remove the radiating fin.</li> <li>Disconnect the 2-pin tungsten lamp connector.</li> <li>Loosen the screws (3) and remove the tungsten lamp.</li> <li>Install the new tungsten lamp.</li> <li>Reconnect the 2-pin tungsten lamp connector.</li> <li>Reinstall the radiating fin.</li> <li>Reinstall the top cover.</li> <li>Reset the lamp operating time [W LAMP USED] and brightness.</li> </ol> <span class="btn-link">[[D2 Lamp Replacement - SPD-20A->Deuterium Lamp Replacement - SPD-20A (1107)]]</span> <span class="btn-link">[[D2 Lamp Replacement - SPD-20AV->D2 Lamp Replacement - SPD-20AV (1124)]]</span> <span class="btn-link">[[D2 Lamp Replacement - SPD-M20A->D2 Lamp Replacement - SPD-M20A (1141) ]]</span> <span class="btn-link">[[W Lamp Replacement - SPD-M20A->W Lamp Replacement - SPD-M20A (1143)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Tungsten Lamp Replacement - SPD-M20A</h1> <div class="center"><iframe width="900" height="506" src="https://www.youtube.com/embed/Qzkhq9xY3m0?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <ol> <li>Turn OFF the power to the instrument and unplug the power cable.</li> <li>Open the doors.</li> <li>Remove the lamp cover.</li> <li>Disconnect the 2-pin tungsten lamp connector.</li> <li>Remove the lamp socket and lamp from the instrument.</li> <li>Remove the old lamp from the lamp socket. Insert the new lamp into the socket.</li> <li>Use the filament positioning plate to verify that the lamp is inserted properly.</li> <li>Reinstall the W lamp assembly.</li> <li>Reconnect the 2-pin connector.</li> <li>Reinstall the lamp cover.</li> <li>Close the doors.</li> <li>Reset the lamp usage time to 0, and perform the exposure time setting.</li> </ol> <span class="btn-link">[[D2 Lamp Replacement - SPD-20A->Deuterium Lamp Replacement - SPD-20A (1107)]]</span> <span class="btn-link">[[D2 Lamp Replacement - SPD-20AV->D2 Lamp Replacement - SPD-20AV (1124)]]</span> <span class="btn-link">[[D2 Lamp Replacement - SPD-M20A->D2 Lamp Replacement - SPD-M20A (1141) ]]</span> <span class="btn-link">[[W Lamp Replacement - SPD-20AV->W Lamp Replacement - SPD-20AV (1126)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>