Nexera HPLC Troubleshooting The Pump

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<div class="container"><h1 class="title">Troubleshooting the Pump</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-pump/lc-front-power.jpg" alt="Troubleshooting the Pump"></div> <h3>Examine the power indicator on the front of the solvent delivery unit.</h3>The power indicator bar is located on the right side of the display panel. <ul> <li>Green indicates that the power is ON.</li> <li>Red indicates that an error has been generated.</li> <li>Orange indicates that the unit is in sleep mode.</li> </ul> <div class="ts-question">Is the power indicator illuminated?</div><div class="buttons"> <span class="btn-yes">[[Yes->Keypad Operation]]</span> <span class="btn-no">[[No->Power Connection - LC]]</span> </div> </div>

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<div class="container"><h1 class="title">Keypad Operation</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-pump/lc-display-key.jpg" alt="Keypad Operation"></div> The Display key is located on the lower right corner of the keypad. This button turns the keypad function ON and OFF <div class="ts-question">Do the keys on the keyboard operate when pressed?</div><div class="buttons"> <span class="btn-yes">[[Yes->LC Pump Operation]]</span> <span class="btn-no">[[No->Keypad - Pump]]</span> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Power Connection - LC</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-pump/lc-power.jpg" alt="Power Connection - LC"></div> <h3>Examine the connections of the power supply cord.</h3>One end of the power cable is inserted into the power connector on the back of the pump. The other end of the power cable is inserted into the power supply outlet. <img class="warning" style="float: left; margin-bottom:30px;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>CAUTION: Ensure that the power switch is in the OFF position before connecting the power cable. WARNING: The three-line type power cable supplied as an accessory includes the grounding wire. Connect this wire to ground to prevent electrical shock and to secure stable operation of the instrument.</strong> <div class="ts-question">Is the power cord properly connected to the pump and power supply outlet?</div> <div class="buttons"> <span class="btn-yes">[[Yes->Power Cord Damage]]</span> <span class="btn-no">[[No->Power Connection - Pump]]</span> <span class="btn-back"><<back "Back">></span> </div>

<div class="container"><h1 class="title">LC Pump Operation</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-pump/lc-pump-key.jpg" alt="LC Pump Operation"></div> <h3>Press the PUMP key to illuminate the PUMP indicator LED.</h3> Press the power switch to turn the pump power ON. Press the power switch again to turn the pump power OFF. Press the PUMP key on the pump keypad to start and stop the pumping action. Press the PUMP key to illuminate the PUMP indicator LED. <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>NOTE: It is possible for power to the pump to be ON while the pumping action is OFF.</strong> <div class="ts-question">Does the pump operate after the PUMP indicator LED is illuminated?</div><div class="buttons"> <span class="btn-yes">[[Yes->Waste Discharge]]</span> <span class="btn-no">[[No->Flow Rate Set to 0 mL/min]]</span> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Keypad - Pump</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-pump/lc-display-key.jpg" alt="LC Pump Operation"></div> The display key is located on the lower right corner of the keypad. This button turns the keypad function ON and OFF. <ol> <li>If the keys are not illuminated, press this key to turn ON the operation keys.</li> <li>If the keys are illuminated, press this key to turn OFF the operation keys.</li> </ol> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Power Cord Damage</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-pump/power-cord-damage.jpg" alt="Power Cord Damage"></div> Handle the power cord with care. Observe the following precautions to avoid the possibility of cord damage that may result in fire, electric shock or instrument malfunction. <ul> <li>Do not place heavy objects on the cord.</li> <li>Keep hot items away from the power cord.</li> <li>Do not modify the power cord in any way.</li> <li>Do not bend the cord excessively.</li> <li>To unplug the cord, pull on the plug itself, NOT on the cord.</li> <li>If the cord becomes damaged, replace it with a new one immediately.</li> </ul> <div class="ts-question">Is the power cord damaged?</div> <div class="buttons"> <span class="btn-yes">[[Yes->Power Cord Replacement]]</span> <span class="btn-no">[[No->Power Supply Requirements - Pump]]</span> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Power Connection - Pump</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-pump/lc-power.jpg" alt="Power Connection - LC"></div> One end of the power cable is inserted into the power connector on the back of the pump. The other end of the power cable is inserted into the power supply outlet. <img class="warning" style="float: left; margin-bottom:30px;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>CAUTION: Ensure that the power switch is in the OFF position before connecting the power cable. WARNING: The three-line type power cable supplied as an accessory includes the grounding wire. Connect this wire to ground to prevent electrical shock and to secure stable operation of the instrument.</strong> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div>

<div class="container"><h1 class="title">Power Cord Replacement</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-pump/power-cord.jpg" alt="Power Cord Replacement"></div> A power supply cable is supplied with each pump. If the cord becomes damaged, replace it with a new one immediately. Visit <a href="https://store.shimadzu.com" target="_blank">our webstore</a> to order parts. <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"> <h1 class="title">Power Supply Requirements - Pump</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-pump/lc-power-req.jpg" alt="Power Supply Requirements - Pump"></div> <h3>Verify that the power outlet has a sufficient capacity.</h3>If the capacity is insufficient, a power outage or voltage drop can occur. Power drops will affect the performance of this instrument and any other instruments that are connected to the same power supply circuit. <div class="ts-question">Does the power supply meet the power supply requirements?</div> <div class="buttons"> <span class="btn-yes">[[Yes->Fuse Inspection - Pump]]</span> <span class="btn-no">[[No->Power Requirements]]</span> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"> <h1 class="title">Fuse Inspection - Pump</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-pump/fuse-inspection.jpg" alt="Fuse Inspection - Pump"></div> <h3>If the circuit has been overloaded, the fuse may blow to protect the system electronics.</h3><ol> <li>Use a coin to open the fuse holders on the back of the pump.</li> <li>Remove the fuses from the fuse holders.</li> <li>Inspect each fuse by holding it up to the light.</li> </ol> <ul> <li>The glass should be clear and clean and the wire should be continuous and extend from one end of the fuse to the other.</li> <li>The fuse is blown and must be replaced if the glass is discolored with black or brown smudges or the wire is clearly broken or not visible at all. </li> </ul> Replacement Part - Fuse 3.15AT (5x20) P/N 072-02004-21 <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>WARNING: Turn the instrument OFF and disconnect the power supply before proceeding.</strong> <div class="ts-question">Have the fuses blown?</div> <div class="buttons"> <span class="btn-yes">[[Yes->Fuse Replacement - LC-30AD]]</span> <span class="btn-no"><a href="https://www.ssi.shimadzu.com/forms/tech-support/index.html?product_category=VirtualAdvisor&product_name=Nexera&page=webform_start">Contact Shimadzu</a></span> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"> <h1 class="title">Power Requirements</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-pump/lc-power-req.jpg" alt="Power Requirements"></div> Ensure that the power supply meets the power supply requirements. If the power supply does not meet the specifications, the instrument may not function properly or become damaged. If the power supply does not meet specifications, take corrective action before connecting this unit to the power circuit. <div class="ts-question">Does the power supply meet the power supply requirements?</div> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"> <h1 class="title">Fuse Replacement - LC-30AD</h1> <div class="center"><iframe width="900" height="506" src="https://www.youtube.com/embed/OhOAMHQZKLU?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>WARNING: Turn the instrument OFF and disconnect the power supply before proceeding.</strong> <br> <ol> <li>Use a coin to open the fuse holders.</li> <li>Remove the fuse holders.</li> <li>Remove the old fuses from the fuse holders.</li> <li>Place new fuses into the fuse holders.</li> <li>Insert the fuse holders back into the instrument.</li> <li>Secure the fuse holders using the coin.</li> </ol> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Waste Discharge</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-pump/waste-discharge.jpg" alt="Waste Discharge"></div> Examine the waste tubing as it enters the waste container. Is the flow of the discharge appropriate for the flow rate? <div class="ts-question">Is there any mobile phase flow?</div><div class="buttons"> <span class="btn-yes">[[Yes->Pressure Factors]]</span> <span class="btn-no">[[No->Loss of Prime]]</span> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Flow Rate Set to 0 mL/min</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-pump/lc-flow-rate.jpg" alt="Flow Rate Set to 0 mL/min"></div> <h3>Examine the flow rate setting</h3> From the Initial screen, press the FUNC key once. The cursor becomes active at the flow rate setting. <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>NOTE: If the flow rate is set to 0 mL/min, the pump will not operate.</strong> <div class="ts-question">Has the flow rate been inadvertently set to 0 mL/min?</div><div class="buttons"> <span class="btn-yes">[[Yes->Pump Power and Flow Rate]]</span> <span class="btn-no">[[No->P-MIN and P-MAX Error Messages]]</span> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Pressure Factors</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-pump/lc-pressure-display.jpg" alt="Pressure Factors"></div> <h3>Observe the pressure display for a period of time.</h3> There are a number of factors that affect the pressure on the output of the pump. Normal pump pressure is a relative term that is dependent on many factors including the following items. <ul> <li>Pump Model- Different pumps will have a different 'normal' pressure based on the pumping mechanism.</li> <li>Mobile Phase - Composition Different solvents have different 'normal' pressures at the same flow rate and with the same pump.</li> <li>Flow Rate - The pressure normally increases as the flow rate increases.</li> </ul> <div class="ts-question">Is the pump pressure stable?</div><div class="buttons"> <span class="btn-yes">[[Yes->Measuring the Flow Rate]]</span> <span class="btn-no">[[No->Loss of Prime]]</span> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Loss of Prime</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-pump/loss-of-prime.jpg" alt="Loss of Prime"></div> <h3>Air bubbles or contamination in the pump heads can cause the pump to malfunction.</h3> The solvent delivery system (pump) must be properly primed in order to function properly. Improper degassing of the mobile phase or leaks in the flow path can allow air to build up in the pump heads and cause a loss of the primed condition. Air bubbles or contamination in the pump heads can cause the pump to malfunction. <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>NOTE: Air bubbles in the drain tubing attached to the drain valve are normal and not a cause for concern.</strong> <div class="ts-question">Have the pump heads been purged recently?</div><div class="buttons"> <span class="btn-yes">[[Yes->Suction Filter Inspection]]</span> <span class="btn-no">[[No->Purging the Pump]]</span> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Pump Power and Flow Rate</h1> <div class="center"><div class="center"><iframe width="900" height="506" src="https://www.youtube.com/embed/CUzqayHkwjs?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div></div> <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>NOTE: It is possible for power to the pump to be ON while the pumping action is OFF.</strong> <ol> <li>Press the power switch to turn the pump power ON.</li> <li>Press the PUMP key on the pump keypad to start and stop the pumping action.</li> <li>From the Initial screen, press the FUNC key one time. The cursor blinks at flow rate.</li> <li>Press 2 - . - 0 - 0 - 0 - 0 keys to set the flow rate to 2.0000 mL per minute.</li> <li>Press ENTER to save the settings.</li> <li>Press CE to return to the Initial screen.</li> </ol> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">P-MIN and P-MAX Error Messages</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-pump/lc-p-min-p-max.jpg" alt="P-MIN and P-MAX Error Messages"></div> <ul> <li>The P-MIN error indicates that the pump discharge pressure has fallen below the set minimum pressure limit, [P.MIN].</li> </ul> The P-MIN error usually indicates that there is a leak in the flow line that is large enough for the pump to have trouble reaching or maintaining the minimum pressure limit. <ul> <li>The P-MAX error indicates that the pump discharge pressure has exceeded the set maximum pressure limit, [P.MAX].</li> </ul> The P-MAX error usually indicates that there is an obstruction in the flow lines such as a clog that is causing the pump to reach a pressure that is above the set maximum pressure limit. <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>NOTE: Each of these errors will cause the pumping action to stop automatically.</strong> <div class="ts-question">Is a P-MIN or P-MAX error being displayed?</div><div class="buttons"> <span class="btn-yes">[[Yes->P-MIN and P-MAX Resolution]]</span> <span class="btn-no"><a href="https://www.ssi.shimadzu.com/forms/tech-support/index.html?product_category=VirtualAdvisor&product_name=Nexera&page=webform_start">Contact Shimadzu</a></span> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Measuring the Flow Rate</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-pump/lc-measuring-the-flow-rate.jpg" alt="Measuring the Flow Rate"></div> <h3>Measure the actual flow rate.</h3><ol> <li>Remove the column and replace it with a flow line union.</li> <li>Set the flow rate to 2.0 mL per minute.</li> <li>Allow the flow rate to stabilize for 5 minutes.</li> <li>Using a stopwatch and graduated cylinder, collect the flow from the drain tubing in the cylinder for 5 minutes.</li> <li>Calculate the volume of mobile phase collected in the 5 minute period.</li> </ol> LC-20AB - The collected volume should be 10.0 mL ± 1%. LC-20AD - The collected volume should be 10.0 mL ± 1%. LC-20AT - The collected volume should be 10.0 mL ± 2%. <div class="ts-question">Is the actual flow rate below the set flow rate?</div><div class="buttons"> <span class="btn-yes">[[Yes->Flow Line Leaks]]</span> <span class="btn-no">[[No->Fluctuating RTs]]</span> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Suction Filter Inspection</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-pump/lc-suction-filter.jpg" alt="Suction Filter Inspection"></div> <h3>Bubbles may be entering the pump from the solvent reservoir.</h3><ul> <li>Ensure that the suction filter tubing is fully inserted into the suction filter.</li> <li>Ensure that the suction filter is fully submerged in the mobile phase or rinse solution.</li> <li>Ensure that the other end of the suction filter tubing is properly connected to the pump inlet and that no leaks are evident.</li> </ul> <div class="ts-question">Examine the pump inlet tubing, are bubbles present in the flow lines?</div><div class="buttons"> <span class="btn-yes">[[Yes->Suction Filter Cleaning]]</span> <span class="btn-no">[[No->Check Valve Flushed]]</span> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Purging the Pump</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-pump/drain-valve.jpg" alt="Purging the Pump"></div> <ol> <li>Fill the mobile phase reservoir with degassed mobile phase.</li> <li>Place the suction filter in the reservoir.</li> <li>Place the end of the drain tubing into the waste container, which has been placed on the floor.</li> <li>Turn the drain valve knob 90° counterclockwise to open the drain valve.</li> <li>Press CE to return to the Initial screen.</li> <li>Verify that the pressure for the displayed valve is in the range of [-0.3 to 0.3 MPa].</li> <li>Press the PURGE key. Pumping begins at the preset flow rate (P-FLOW).</li> <li>Allow the purge to run until the mobile phase flows from the drain tubing in a continuous and air free stream.</li> <li>Press the PURGE key to stop the pump.</li> </ol> <img class="warning" style="float: left; margin-bottom:30px;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>NOTE: Air bubbles in the drain tubing attached to the drain valve are normal and not a cause for concern. NOTE: If no mobile phase comes from the drain tubing, insert the tip of the supplied needle into the end of the drain tubing and using an attached syringe, draw the mobile phase through the tubing.</strong> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">P-MIN and P-MAX Resolution</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-pump/lc-p-min-p-max.jpg" alt="P-MIN and P-MAX Resolution"></div> If the P-MIN error has been displayed, check the following items. <ul> <li>Ensure that there is a column or restrictor tubing in the flow path of the pump output.</li> <li>Ensure that the drain valve was not left open.</li> <li>Verify that the flow line connections are not leaking.</li> </ul> If the P-MAX error has been displayed, check the following items. <ul> <li>Ensure that the column is not blocked by replacing the column with restrictor tubing. If the column is blocked, flush it to remove the blockage or replace it with a similar column.</li> <li>Ensure that the flow lines are not blocked. If the flow lines are blocked, flush them or replace them as necessary.</li> </ul> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Flow Line Leaks</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-pump/flow-lines.jpg" alt="Flow Line Leaks"></div> <h3>Examine the flow line connections for leaks.</h3>A leak in the flow line before the detector flow cell could explain the absence of sample peaks. Visually inspect the flow line from needle to flow cell looking for signs of a leak: <ul> <li>Puddles</li> <li>Drips</li> <li>Crystallization of buffer</li> </ul> <div class="ts-question">Is liquid leaking from the flow line connections?</div><div class="buttons"> <span class="btn-yes">[[Yes->Tubing - Flow Line Connections]]</span> <span class="btn-no">[[No->Suction Filter Inspection]]</span> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Fluctuating RTs</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-pump/flow-resolution.jpg" alt="Fluctuating RTs"></div> <h3>Compare the chromatography from multiple injections.</h3>The flow rate can have a moderate affect on resolution. Refer to the column information to select the appropriate flow rate. <div class="ts-question">Are the peak retention times fluctuating?</div><div class="buttons"> <span class="btn-yes">[[Yes->Check Valve Flushed]]</span> <span class="btn-no">[[No->Monitoring the Pressure Display]]</span> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Tubing - Flow Line Connections</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-pump/tubing-flow-line-connections.jpg" alt="Tubing - Flow Line Connections"></div> <ol> <li>Place a male nut and ferrule on the tubing.</li> <li>Insert the end of the tubing with the ferrule completely into the appropriate fitting.</li> <li>Push the tubing into the fitting until it butts up against the end of the fitting (tubing stop position).</li> <li>While making sure the tubing remains in the tubing stop position, securely tighten the male nut. The ferrule is secured to the tubing.</li> </ol> <img class="warning" style="float: left; " src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>NOTE: It is important to apply a significant amount of pressure to the tubing while tightening the male nut.</strong> <span class="btn-link">[[Cutting Resin ->Cutting Resin]]</span> <span class="btn-link">[[Cutting SS ->Cutting SS]]</span> <span class="btn-link">[[Tighten SS ->Tighten SS]]</span> <div class="buttons"><span class="btn-back"><<back "Back">></span></div> </div>

<div class="container"><h1 class="title">Check Valve Flushed</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-pump/lc-check-valve.jpg" alt="Check Valve Flushed"></div> The check valves used in the solvent delivery units are two-way valves that allow fluid to flow in only one direction. They incorporate a small spherical ball that moves into and out of a conical seat to open or close the valve opening. <ul> <li>If dirt or fluid residues are allowed to buildup inside the check valve, the ball may no longer be able to seal the valve opening. When this happens, the check valves need to be flushed or sonicated to clean them.</li> <li>After prolonged use, the check valves may eventually wear to a point where the ball will no longer seal the valve opening. When this happens, the check valves need to be replaced.</li> </ul> <div class="ts-question">Have the check valves been flushed with 2-propanol to remove any buildup that may have accumulated?</div><div class="buttons"> <span class="btn-yes">[[Yes->Check Valve Sonicated]]</span> <span class="btn-no">[[No->Check Valve Cleaning]]</span> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Monitoring the Pressure Display</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-pump/lc-pressure-display.jpg" alt="Monitoring the Pressure Display"></div> <h3>Turn the pump ON and monitor the pressure display.</h3>Allow the pump to run for a short while to achieve a constant level of flow through the flow lines. Watch the pump display screen and determine the level of pressure fluctuation. If the pressure does not rise, it may indicate one of the following. <ul> <li>Drain valve may be OPEN</li> <li>No load (column) on the output</li> <li>Major leak in the flow line path</li> </ul> If the pressure rises too much, it may indicate one of the following. <ul> <li>There is a clog in a flow line</li> <li>The column is clogged</li> <li>The flow line is crimped</li> </ul> <div class="ts-question">Does the pressure rise with the pumping action?</div><div class="buttons"> <span class="btn-yes">[[Yes->Pressure Too High]]</span> <span class="btn-no">[[No->Drain Valve Open]]</span> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Tubing - Cutting Resin</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-pump/tubing-cutting-resin.jpg" alt="Tubing - Cutting Resin"></div> When using PEEK tubing or equivalent resin tubing, use a straight edge razor knife to cut the tubing. To avoid the creation of void volumes, verify that the cut edges are clean right-angled cuts. <span class="btn-link">[[Flow Line Connections ->Tubing - Flow Line Connections]]</span> <span class="btn-link">[[Cutting SS ->Cutting SS]]</span> <span class="btn-link">[[Tighten SS ->Tighten SS]]</span> <div class="buttons"><span class="btn-back"><<back "Back">></span></div> </div>

<div class="container"><h1 class="title">Tubing - Cutting SS</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-pump/tubing-cutting-ss.jpg" alt="Tubing - Cutting SS"></div> When using HPLC stainless steel tubing, it is best to use precut stainless steel tubing or employ a cutting technique that produces a smooth and even surface to the cut edge. If the cut edge of the tubing is not smooth and square, a void volume is created in the connection. <span class="btn-link">[[Cutting Resin ->Cutting Resin]]</span> <span class="btn-link">[[Flow Line Connections ->Tubing - Flow Line Connections]]</span> <span class="btn-link">[[Tighten SS ->Tighten SS]]</span> <div class="buttons"><span class="btn-back"><<back "Back">></span></div> </div>

<div class="container"><h1 class="title">Tubing - Tighten SS</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-pump/tubing-tighten-ss.jpg" alt="Tubing - Tighten SS"></div> Verify that the stainless steel unions are securely connected. Use two wrenches to tighten stainless steel couplings. <span class="btn-link">[[Flow Line Connections ->Tubing - Flow Line Connections]]</span> <span class="btn-link">[[Cutting SS ->Cutting SS]]</span> <span class="btn-link">[[Cutting Resin ->Cutting Resin]]</span> <div class="buttons"><span class="btn-back"><<back "Back">></span></div> </div>

<div class="container"><h1 class="title">Suction Filter Cleaning</h1> <div class="center"><iframe width="900" height="506" src="https://www.youtube.com/embed/AtJZZ2rca2A?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <ol> <li>Pull the suction filter out of the suction tubing.</li> <li>Sonicate the suction filter in 2-propanol for 5 minutes.</li> <li>Insert the suction tubing into the suction filter.</li> <li>Turn the power switch ON to display the initial screen.</li> <li>Pump water at 1 mL/min for 10 minutes.</li> <li>If air bubbles accumulate inside the solvent tubing, the tubing must be replaced.</li> </ol> <img class="warning" style="float: left; " src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>NOTE: Sometimes it is beneficial to shake the suction filter rapidly to dislodge any trapped air bubbles.</strong> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Check Valve Sonicated</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-pump/lc-check-valve.jpg" alt="Check Valve Sonicated"></div> The check valves used in the solvent delivery units are two-way valves that allow fluid to flow in only one direction. They incorporate a small spherical ball that moves into and out of a conical seat to open or close the valve opening. <ul> <li>If dirt or fluid residues are allowed to buildup inside the check valve, the ball may no longer be able to seal the valve opening. When this happens, the check valves need to be flushed or sonicated to clean them.</li> <li>After prolonged use, the check valves may eventually wear to a point where the ball will no longer seal the valve opening. When this happens, the check valves need to be replaced.</li> </ul> <div class="ts-question">Have the check valves been removed and cleaned with sonication?</div> <div class="buttons"> <span class="btn-yes"><a href="https://www.ssi.shimadzu.com/forms/tech-support/index.html?product_category=VirtualAdvisor&product_name=Nexera&page=webform_start">Contact Shimadzu</a></span> <span class="btn-no">[[No->Check Valve Cleaning and Replacement]]</span> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Check Valve Cleaning</h1> <div class="center"><iframe width="900" height="506" src="https://www.youtube.com/embed/A4MMpD-Irgw?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <ol> <li>Replace the mobile phase with 2-propanol.</li> <li>Remove the column from the flow line and attach a restrictor tube in its place.</li> <li>Pump 2-propanol through pump A at 2mL/min for about 1 hour.</li> <li>Pump 2-propanol through pump B at 2mL/min for about 1 hour.</li> <li>Replace the 2-propanol with mobile phase and flush the lines.</li> <li>Remove the restrictor tube and reattach the column.</li> </ol> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Check Valve Cleaning and Replacement</h1> <div class="center"><iframe width="900" height="506" src="https://www.youtube.com/embed/3YCld2M2Gzo?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <img class="warning" style="float: left; " src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>CAUTION: Do not disassemble the check valves for any reason. If they are disassembled, their performance may be affected. Do not invert the inlet check valve. Doing so will cause internal parts to fall apart..</strong> <ol> <li>Open and remove the door.</li> <li>Place the reservoir lower than the pump inlet.</li> <li>Use the provided wrench to hold the Outlet check valve.</li> <li>Use the other wrench to loosen the male nuts at both ends of the drain tubing.</li> <li>Twist the drain tubing out of the Outlet check valve.</li> <li>Remove the male PEEK from the Inlet check valve.</li> <li>Use the provided wrench to remove the check valve IN-UHP and OUT-UHP.</li> <li>Sonicate the check valves IN-UHP and OUT-UHP in 2-propanol for 5 minutes.</li> <li>Screw the check valves IN-UHP and OUT-UHP back into the pump head.</li> <li>Hand-tighten them then use a wrench to turn them an additional 90 degrees.</li> <li>Reconnect the male PEEK to the Inlet check valve.</li> <li>Twist the drain tubing back into the Outlet check valve.</li> <li>Use the provided wrenches to secure the male nuts at both ends of the drain tubing.</li> <li>Return the reservoir bottle to the original state. Reinstall and close the door.</li> </ol> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Pressure Too High</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-pump/lc-pressure-display.jpg" alt="Pressure Too High"></div> <h3>Turn the pump ON and monitor the pressure display.</h3>Allow the pump to run for a short while to achieve a constant level of flow through the flow lines. Watch the pump display screen and determine the level of pressure fluctuation. If the pressure does not rise, it may indicate one of the following. <ul> <li>Drain valve may be OPEN</li> <li>No load (column) on the output</li> <li>Major leak in the flow line path</li> </ul> If the pressure rises too much, it may indicate one of the following. <ul> <li>There is a clog in a flow line</li> <li>The column is clogged</li> <li>The flow line is crimped</li> </ul> Turn the pump ON and monitor the pressure display. <div class="ts-question">Does the pressure rise too much during the pumping process?</div><div class="buttons"> <span class="btn-yes">[[Yes->Check Valve Flushed]]</span> <span class="btn-no">[[No->Rinse Volume Change]]</span> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Drain Valve Open</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-pump/drain-valve.jpg" alt="Drain Valve Open"></div> <h3>Examine the drain valve knob.</h3>During normal pump operation, the drain valve is closed. However, the valve can be opened to replace the mobile phase in the flow lines or to bleed air from the flow lines. If the valve is not fully closed after these operations, the flow line remains open and mobile phase does not flow to the column. Examine the drain valve knob. <div class="ts-question">Was the drain valve inadvertently left open?</div><div class="buttons"> <span class="btn-yes">[[Yes->Drain Valve Replacement]]</span> <span class="btn-no">[[No->Output Restriction]]</span> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Rinse Volume Change</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-pump/automatic-rinse-kit.jpg" alt="Rinse Volume Change"></div> <h3>If an automatic rinse kit is installed, monitor the volume of the rinse solution.</h3>The automatic rinsing kit is an option for the LC-20AB and LC-20AD solvent delivery units. This option allows for automatic and continual rinsing of the plungers and plunger seals inside the pump heads of the pump. <div class="ts-question">Is the volume of the rinse solution changing?</div><div class="buttons"> <span class="btn-yes">[[Yes->Increase or Decrease]]</span> <span class="btn-no"><a href="https://www.ssi.shimadzu.com/forms/tech-support/index.html?product_category=VirtualAdvisor&product_name=Nexera&page=webform_start">Contact Shimadzu</a></span> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Drain Valve Replacement</h1> <div class="center"><iframe width="900" height="506" src="https://www.youtube.com/embed/sk-GCHXTzSw?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>NOTE: If the drain valve ASSY is forced in at an angle, the seal may be damaged. Any damage can compromise the seal.</strong> <ol> <li>Open the door</li> <li>Unscrew the drain valve ASSY by turning counterclockwise and pull straight out of the drain valve</li> <li>Wet the seal of the new drain valve ASSY by using the 2-propanol.</li> <li>Insert the drain valve ASSY into the hole of the housing in a straight line.</li> <li>Screw the new drain valve ASSY as far as it will go to close</li> <li>Screw the knob to the drain valve ASSY and insert the cap into the knob while the knob position is vertical.</li> <li>Close the door.</li> </ol> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Output Restriction</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-pump/lc-output-restriction.jpg" alt="Output Restriction"></div> It is necessary for there to be a restriction on the output of the pump in order for the pump to register a pressure. If the output flow line is open without restriction, the pressure will not rise. Ensure that there is a column or restriction tubing attached to the pump output to get an accurate system pressure reading. <div class="ts-question">Is there a restriction on the pump output such as a column or restrictor tubing?</div><div class="buttons"> <span class="btn-yes">[[Yes->Flow Line Leaks]]</span> <span class="btn-no">[[No->Flow Line Restriction]]</span> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Flow Line Restriction</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-pump/lc-output-restriction.jpg" alt="Flow Line Restriction"></div> It is necessary for there to be a restriction on the output of the pump in order for the pump to register a pressure. <ol> <li>Verify that the drain valve is closed.</li> <li>Connect a column or restrictor tubing to the pump output.</li> <li>Ensure that there are no leaks in the flow line connections throughout the entire system flow path.</li> <li>Monitor the pressure to verify that the pressure rises.</li> </ol> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Increase or Decrease</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-pump/automatic-rinse-kit.jpg" alt="Increase or Decrease"></div> <ul> <li>Generally, if the volume of rinse solution increases during pumping, the plunger seal is leaking. Remove the rinsing kit, identify whether the leak is in the left or right plunger seal and replace the appropriate seal.</li> <li>If the volume of rinse solution decreases during pumping, the diaphragm is leaking. Confirm that the leak is coming from the bottom of the head holder, and replace the diaphragm.</li> </ul> <div class="ts-question">Is the rinse volume increasing or decreasing?</div> <div class="buttons"> <span class="btn-yes">[[Increasing->Plunger and Diaphragm Removal]]</span> <span class="btn-no">[[Decreasing->Plunger Seal Replacement - LC30AD]]</span> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Plunger and Diaphragm Removal</h1> <div class="center"><iframe width="900" height="506" src="https://www.youtube.com/embed/CZh0frvCfk?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>CAUTION: Gently push the head holder on, keeping it aligned with the plunger at all times. If it is pushed in forcefully, the plunger could be damaged.</strong> <ol> <li>Turn the power switch ON. The Initial screen appears.</li> <li>Press [func] (x6) to display the [CONTROL] menu.</li> <li>Press [enter] to display the [P-SET] screen.</li> <li>Press [2] and [enter] to retract the right plunger.(Press [1] and [enter] for the left plunger.)</li> <li>Press [CE] two times to display the Initial screen.</li> <li>Place the reservoir lower than the pump inlet.</li> <li>Open and remove the door.</li> <li>Use the provided wrench to hold the Outlet check valve.</li> <li>Use the other wrench to loosen the male nuts at both ends of the drain tubing.</li> <li>Twist the drain tubing out of the Outlet check valve.</li> <li>Remove the male PEEK nut from the Inlet check valve.</li> <li>Alternately loosen the pump head retaining bolts with the provided Allen wrench.</li> <li>Remove the pump head retaining bolts.</li> <li>Remove the pump head.</li> <li>Remove the back-up ring from the plunger.</li> <li>Use tweezers to remove the rinse tubing from the pump head holder.</li> <li>Alternately loosen the pump head holder retaining bolts with the provided Allen wrench.</li> <li>Remove the pump head holder retaining bolts.</li> <li>Carefully remove the pump head holder.</li> <li>Using the box driver, rotate the plunger holder counterclockwise, and remove it.</li> </ol> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Plunger Seal Replacement - LC30AD</h1> <div class="center"><iframe width="900" height="506" src="https://www.youtube.com/embed/IGblq3AlpKM?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>CAUTION: If the pump head is pulled out or pushed in diagonally, the plunger could be broken.</strong> <ol> <li>Turn the power switch ON. The Initial screen appears.</li> <li>Press [func] (x6) to display the [CONTROL] menu.</li> <li>Press [enter] to display the [P-SET] screen.</li> <li>Press [2] and [enter] to retract the right plunger.(Press [1] and [enter] for the left plunger.)</li> <li>Press [CE] two times to display the Initial screen.</li> <li>Place the reservoir lower than the pump inlet.</li> <li>Open and remove the door.</li> <li>Use the provided wrench to hold the Outlet check valve.</li> <li>Use the other wrench to loosen the male nuts at both ends of the drain tubing.</li> <li>Twist the drain tubing out of the Outlet check valve.</li> <li>Remove the male PEEK from the Inlet check valve.</li> <li>Alternately loosen the pump head retaining bolts with the provided Allen wrench.</li> <li>Remove the pump head retaining bolts and remove the pump head.</li> <li>Use the flanged end of the seal installer - remover to remove the seal from the pump head.</li> <li>Open the new seal and backup ring.</li> <li>Use the straight end of the seal installer - remover to install the new plunger seal.</li> <li>Place the backup ring on the plunger seal.</li> <li>Install the pump head onto the head holder so that "130" is right-side up. Do not to allow the backup ring to fall out of the head.</li> <li>Insert the two hex socket head and tighten them in alternately and uniformly (90° at a time) with the Allen wrench.</li> <li>Re-connect the tubing, close the door and return the reservoir to the original position.</li> </ol> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>