Nexera HPLC Troubleshooting the Column Oven

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<div class="container"><h1 class="title">Troubleshooting the Column Oven</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-column-oven/cto-power-indicator.jpg" alt="Troubleshooting the Column Oven"></div> <h3>Examine the power indicator on the front of the column oven.</h3> The power indicator bar is located on the right side of the display panel. <ul> <li>Green indicates that the power is ON.</li> <li>Red indicates that an error has been generated.</li> <li>Orange indicates that the unit is in sleep mode.</li> </ul> Examine the power indicator on the front of the column oven. <div class="ts-question">Is the indicator illuminated?</div><div class="buttons"> <span class="btn-yes">[[Yes->Display Key - CTO (1998)]]</span> <span class="btn-no">[[No->Power Connection - CTO (1988)]]</span> </div> </div>

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<div class="container"><h1 class="title">Display Key - CTO</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-column-oven/keypad-cto.jpg" alt="Display Key - CTO"></div> The display key is located on the lower right corner of the keypad. This button turns the keypad operation keys ON and OFF. <div class="ts-question">Do the keys on the keyboard operate when pressed?</div><div class="buttons"> <span class="btn-yes">[[Yes->READY Indicator - CTO (2015)]]</span> <span class="btn-no">[[No->Display Key - CTO 2 (2000)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Power Connection - CTO</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-column-oven/power-cto.jpg" alt="Power Connection - CTO"></div> One end of the power cable is inserted into the power connector on the back of the column oven. The other end of the power cable is inserted into the power supply outlet. <img class="warning" style="float: left; margin-bottom:25px;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>CAUTION: Ensure that the power switch is in the OFF position before connecting the power cable. WARNING: The three-line type power cable supplied as an accessory includes the grounding wire. Connect this wire to ground to prevent electrical shock and to secure stable operation of the instrument.</strong> <div class="ts-question">Is the power cord properly connected to the column oven and power supply outlet?</div><div class="buttons"> <span class="btn-yes">[[Yes->Power Cord Damage (1991)]]</span> <span class="btn-no">[[No->Making Power Connection - CTO (1990)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">READY Indicator - CTO</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-column-oven/cto-ready-indicator.jpg" alt="READY Indicator - CTO"></div> The READY indicator LED is located on the right side of the display panel. This indicator turns ON when the difference in the actual oven temperature and the set temperature stays within the READY RANGE for longer than the WAIT TIME. <div class="ts-question">Is the READY indicator illuminated?</div> <div class="buttons"> <span class="btn-yes">[[Yes->Leak Sensor - CTO (2036)]]</span> <span class="btn-no">[[No->OVEN Indicator - CTO (2023)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Display Key - CTO</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-column-oven/keypad-cto.jpg" alt="Display Key - CTO"></div> The display key is located on the lower right corner of the keypad. This button turns the keypad operation keys ON and OFF. <ol> <li>If the operation keys are not illuminated, press this key to turn ON the operation keys.</li> <li>If the operation keys are illuminated, press this key to turn OFF the operation keys.</li> </ol> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Power Cord Damage</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-column-oven/power-cord-damage.jpg" alt="Power Cord Damage"></div> Handle the power cord with care. Observe the following precautions to avoid the possibility of cord damage that may result in fire, electric shock or instrument malfunction. <ul> <li>Do not place heavy objects on the cord.</li> <li>Keep hot items away from the power cord.</li> <li>Do not modify the power cord in any way.</li> <li>Do not bend the cord excessively.</li> <li>To unplug the cord, pull on the plug itself, NOT on the cord.</li> <li>If the cord becomes damaged, replace it with a new one immediately.</li> </ul> <div class="ts-question">Is the power cord damaged?</div><div class="buttons"> <span class="btn-yes">[[Yes->Power Cord Replace (1992)]]</span> <span class="btn-no">[[No->Power Requirements - CTO (1994)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Making Power Connection - CTO</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-column-oven/power-cto.jpg" alt="Making Power Connection - CTO"></div> <ol> <li>Turn the power switch on the column oven OFF before plugging the instrument into the power supply.</li> <li>Insert the plug end of the power cable directly into the power supply outlet.</li> <li>Insert the connector end of the power cable into the power connector on the back of the column oven.</li> </ol> <img class="warning" style="float: left; margin-bottom:25px;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>WARNING: The three-line type power cable supplied as an accessory includes the grounding wire. Connect this wire to ground to prevent electrical shock and to secure stable operation of the instrument.</strong> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Leak Sensor - CTO</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-column-oven/leak-sensor.jpg" alt="Leak Sensor - CTO"></div> The leak sensor in the column oven is different from those in the other LC-30A series units. This leak sensor monitors and detects the presence of organic vapor. Mobile phase generally contains some amount of organic solvent that vaporizes either at room temperature or at elevated temperatures. Since the column oven chamber remains sealed during operation, the vapor from any leaked solvent is detected by the vapor leak sensor. The leak sensor is located behind the screen inside the main oven chamber. If the value of the leak sensor signal exceeds the LEAK THR, an error is generated, and temperature regulation stops. The sensitivity threshold of the leak sensor needs to be adjusted if an error message fails to appear during a known mobile phase leak, or if an error message appears when there is no leak. <div class="ts-question">Is the problem related to the leak sensor?</div> <div class="buttons"> <span class="btn-yes">[[Yes->Leak Sensor Adjustment - CTO (2038)]]</span> <span class="btn-no">[[No->COOLER MODE - CTO (2046)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">OVEN Indicator - CTO</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-column-oven/cto-oven-indicator.jpg" alt="OVEN Indicator - CTO"></div> The OVEN indicator LED is located on the left side of the display panel. This indicator illuminates at the start of temperature regulation. <img class="warning" style="float: left; margin-bottom:25px;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>NOTE: If the column oven door is opened, temperature regulation stops and the OVEN indicator LED blinks. Temperature regulation resumes when the column oven door is closed.</strong> <div class="ts-question">Is the OVEN indicator illuminated?</div> <div class="buttons"> <span class="btn-yes">[[Yes->COOLER MODE - CTO (2028)]]</span> <span class="btn-no">[[No->OVEN Key - CTO (2025)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">COOLER MODE - CTO</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-column-oven/cooler-mode-cto.jpg" alt="COOLER MODE - CTO"></div> The CTO-30AC model is equipped with a heater as well as a cooler that enables regulation of the temperature of the oven chamber and flow lines to temperatures slightly below room temperature. There are three different modes of operation for the cooler. 0 - Manual OFF Use this mode for applications where it is known that the cooler will not be used. 1 - Manual ON Use this mode for applications where it is known that the cooler will be used. 2 - Auto Operation Use this mode for applications where the set temperature is relatively close to the ambient temperature <div class="ts-question">Is the COOLER MODE set to 0?</div> <div class="buttons"> <span class="btn-yes">[[Yes->Setting the COOLER MODE (10501)]]</span> <span class="btn-no">[[No->Temperature - CTO (2032)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">OVEN Key - CTO</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-column-oven/oven-key-cto.jpg" alt="OVEN Key - CTO"></div> The OVEN key is located in the upper right corner of the keypad. <ol> <li>Press the OVEN key to initiate temperature regulation.</li> <li>Press the key again to stop temperature regulation.</li> </ol> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Leak Sensor Adjustment - CTO</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-column-oven/leak-sensor.jpg" alt="Leak Sensor Adjustment - CTO"></div> The leak sensor in the column oven monitors and detects the presence of organic vapor. The sensitivity of the leak sensor can be adjusted to prevent false errors or to ensure the detection of an actual leak. It may be necessary to replace the leak sensor if the threshold is set to the maximum setting and is still not detecting leaks. <div class="ts-question">Has the leak sensor been adjusted recently?</div> <div class="buttons"> <span class="btn-yes"><a href="https://www.ssi.shimadzu.com/forms/tech-support/index.html?product_category=VirtualAdvisor&product_name=Nexera&page=webform_start">Contact Shimadzu</a></span> <span class="btn-no">[[No->Leak Sensor Adjustment - CTO (11863)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">COOLER MODE - CTO</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-column-oven/cto-cooler-mode.jpg" alt="COOLER MODE - CTO"></div> The CTO-30AC model is equipped with a heater as well as a cooler that enables regulation of the temperature of the oven chamber and flow lines to temperatures slightly below room temperature. There are three different modes of operation for the cooler. 0 - Manual OFF Use this mode for applications where it is known that the cooler will not be used. 1 - Manual ON Use this mode for applications where it is known that the cooler will be used. 2 - Auto Operation Use this mode for applications where the set temperature is relatively close to the ambient temperature. <div class="ts-question">Does the oven temperature fall to room temperature?</div> <div class="buttons"> <span class="btn-yes"><a href="https://www.ssi.shimadzu.com/forms/tech-support/index.html?product_category=VirtualAdvisor&product_name=Nexera&page=webform_start">Contact Shimadzu</a></span> <span class="btn-no">[[No->Installation Clearance - CTO (2048)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Power Cord Replace</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-column-oven/power-cord.jpg" alt="Power Cord Replace"></div> A power supply cable is supplied with each detector. If the cord becomes damaged, replace it with a new one immediately. Visit <a href="https://store.shimadzu.com" target="_blank">our webstore</a> to order parts. <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Power Requirements - CTO</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-column-oven/cto-power-req-small.jpg" alt="Power Requirements - CTO"></div> Verify that the power outlet has a sufficient capacity. If capacity is insufficient, a power outage or voltage drop can occur. Power drops will affect the performance of this instrument and any other instruments that are connected to the same power supply circuit. <div class="ts-question">Does the power supply meet the power supply requirements?</div> <div class="buttons"> <span class="btn-yes"><a href="https://www.ssi.shimadzu.com/forms/tech-support/index.html?product_category=VirtualAdvisor&product_name=Nexera&page=webform_start">Contact Shimadzu</a></span> <span class="btn-no">[[No->Power Requirements - CTO (1996)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Leak Sensor Adjustment - CTO</h1> <div class="center"><iframe width="900" height="506" src="https://www.youtube.com/embed/oKC8-Rgog-M?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <ol> <li>From the initial screen, press ‘VP’ until the [CALIBRATION] screen is displayed.</li> <li>Press ‘func’.</li> <li>The [Input PASSWORD] screen is displayed. Input the password.</li> <li>When the password matches, the [TEMP-R1 CAL] screen is displayed.</li> <li>Press ‘func’ until the [LEAK THR] screen is displayed.</li> <li>Use the numeric keypad to set a sufficiently large value for the threshold value (generally between 200 and 255).</li> <li>Use a syringe filled with water to dampen the thermosensor below the leak sensor.</li> <li>Record the maximum Lv value of the leak sensor. Here, the maximum value is [148].</li> <li>Use the numeric keypad to set the threshold to a value that is 8-10 points lower than the Lv value.</li> <li>Press ‘enter’ to save the value permanently.</li> <li>Run the [LEAK SENSOR TEST] and if [SENSOR GOOD] appears, adjustment is complete.</li> </ol> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Installation Clearance - CTO</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-column-oven/sil-30-install.jpg" alt="Installation Clearance - CTO"></div> Ensure that at least 100 mm of space is left between the rear of the unit and the wall. This space provides sufficient air flow for the cooling of the unit fans and allows the instrument to perform properly. If this space is not sufficient, the instrument may overheat and interfere with the instrument performance. <div class="ts-question">Is there sufficient clearance around the column oven?</div> <div class="buttons"> <span class="btn-yes">[[Yes->Ambient Temperature Sensor Placement - CTO (2051)]]</span> <span class="btn-no">[[No->Installation Clearance - CTO (2049)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Ambient Temperature Sensor Placement - CTO</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-column-oven/ambient-temperature-sensor.jpg" alt="Ambient Temperature Sensor Placement - CTO"></div> <ul> <li>The ambient temperature sensor is connected to the AMB.T connector on the back of the column oven.</li> <li>The clamp is used to secure the sensor to a location near the instrument with minimal temperature fluctuation.</li> <li>Ensure that there is ample room for air circulation behind the LC system so the sensor accurately reads the ambient temperature.</li> </ul> <div class="ts-question">Is the ambient temperature behind the column oven high?</div> <div class="buttons"> <span class="btn-yes">[[Yes->Ambient Temperature Sensor Placement Procedure - CTO (2053)]]</span> <span class="btn-no"><a href="https://www.ssi.shimadzu.com/forms/tech-support/index.html?product_category=VirtualAdvisor&product_name=Nexera&page=webform_start">Contact Shimadzu</a></span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Installation Clearance - CTO</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-column-oven/sil-30-install.jpg" alt="Installation Clearance - CTO"></div> Provide that at least 100 mm of space is left between the rear of the unit and the wall. This space provides sufficient air flow for the cooling of the unit fans and allows the instrument to perform properly. If this space is not sufficient, the instrument may overheat and interfere with the instrument performance. <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Ambient Temperature Sensor Placement Procedure - CTO</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-column-oven/ambient-temperature-sensor.jpg" alt="Ambient Temperature Sensor Placement Procedure - CTO"></div> The ambient temperature sensor is connected to the AMB.T connector on the back of the column oven. <ol> <li>Use the clamp to secure the sensor to a location near the instrument with minimal temperature fluctuation.</li> <li>Provide ample room for air circulation behind the LC system so the sensor accurately reads the ambient temperature.</li> <li>Remove any sources of heat that may be causing the ambient temperature to be higher behind the column oven.</li> </ol> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Setting the COOLER MODE</h1> <div class="center"><iframe width="900" height="506" src="https://www.youtube.com/embed/1jtDuZtvGZE?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <ol> <li>From the Initial screen, press the FUNC key until the SYSTEM screen is displayed.</li> <li>Press ENTER. The Local screen is displayed.</li> <li>Press FUNC until the COOLER MODE screen is displayed.</li> <li>Verify that the COOLER MODE is set to 1 or 2 to ensure that the cooler operation is activated.</li> </ol> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Temperature - CTO</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-the-column-oven/column-temp.jpg" alt="Temperature - CTO"></div> Some constraints arise when choosing the set temperature. <ul> <li>The CTO-30A column oven is able to achieve a temperature of at least ambient temperature plus 10°C up to 85°C. This column oven does not come equipped with a cooler so it is not able to cool below the ambient temperature or reduce the column oven temperature if it should go above the set temperature.</li> <li>The CTO-30AC is able to maintain temperatures of ambient temperature minus 10°C to 85°C. This column oven is equipped with a cooler so it is able to cool the column temperature to 10°C less than the room temperature and maintain the set temperature above room temperature.</li> </ul> Consider these limitations when choosing a set temperature for the column oven. <div class="ts-question">Considering the ambient temperature, is the set temperature appropriate?</div> <div class="buttons"> <span class="btn-yes"><a href="https://www.ssi.shimadzu.com/forms/tech-support/index.html?product_category=VirtualAdvisor&product_name=Nexera&page=webform_start">Contact Shimadzu</a></span> <span class="btn-no">[[No->Setting the Temperature (10503)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Setting the Temperature</h1> <div class="center"><iframe width="900" height="506" src="https://www.youtube.com/embed/lLyenJrQ--U?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> The temperature setting screen may also be displayed by pressing the TEMP key. This temperature is displayed as the set temperature on the Initial screen. This setting remains in memory after the power is turned off. <ol> <li>From the Initial screen, press the FUNC key. The PARAMETER screen is displayed.</li> <li>Press ENTER. This displays the SET TEMP screen.</li> <li>Enter the desired operating temperature (4-85°). 40°C is used here for this example.</li> <li>Press 4 - 0 and the ENTER key.</li> <li>Press CE twice to return to the Initial screen.</li> </ol> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>