Nexera HPLC Troubleshooting - The PDA Detector

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Lamp Intensity Check-- https://nexerava.ssi.shimadzu.com/node/1472 https://nexerava.ssi.shimadzu.com/node/1423 Flow cell contamination https://nexerava.ssi.shimadzu.com/node/1426 https://nexerava.ssi.shimadzu.com/node/1464 merged with https://nexerava.ssi.shimadzu.com/node/1480 Column Insulation https://nexerava.ssi.shimadzu.com/node/1496 https://nexerava.ssi.shimadzu.com/node/1452 Column Oven - triple merged https://nexerava.ssi.shimadzu.com/node/1453 - 30series https://nexerava.ssi.shimadzu.com/node/1497 - 20 series https://nexerava.ssi.shimadzu.com/node/1495 - 30 series Vibration https://nexerava.ssi.shimadzu.com/node/1455 https://nexerava.ssi.shimadzu.com/node/1466

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<div class="container"><h1 class="title">Troubleshooting the PDA Detector</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector/PowerIndicator_a.jpg" alt="Troubleshooting the PDA Detecetor"></div> <h3>Examine the power indicator on the front of the detector..</h3> The power indicator bar is located on the right side of the display panel. <ul> <li>Green indicates that the power is ON.</li> <li>Red indicates that an error has been generated.</li> <li>Orange indicates that the unit is in sleep mode.</li> </ul> <div class="ts-question">Is the indicator illuminated?</div><div class="buttons"> <span class="btn-yes">[[Yes->Lamp Intensity Check(1423)]]</span> <span class="btn-no">[[No->Plugged In (1393)]]</span> </div> </div>

<div class="container"><h1 class="title">Plugged In</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector/Power_SPD.jpg" alt="Troubleshooting the PDA Detecetor"></div> One end of the power cable is inserted into the power connector on the back of the detector. The other end of the power cable is inserted into the power supply outlet. <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>CAUTION: Ensure that the power switch is in the OFF position before connecting the power cable. WARNING: The three-line type power cable supplied as an accessory includes the grounding wire. Connect this wire to ground to prevent electrical shock and to secure stable operation of the instrument.</strong> <div class="ts-question">Is the power cord properly connected to the detector and power supply outlet?</div> <div class="buttons"> <span class="btn-yes">[[Yes->Power Cord Damage(1399)]]</span> <span class="btn-no">[[No->Making the Power Connection (1397)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Lamp Intensity Check</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector/LampIntensityCheckLCSolution.jpg" alt="Lamp Intensity Check"></div> <h3>Record the lamp intensities for future reference.</h3> <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>D2 - maximum intensity at or around 235 nm ≥ 7V<br> W - maximum intensity at or around 550-600 nm ≥ 7V</strong> <div class="ts-question">Is the lamp energy lower than expected?</div> <span class="btn-link">[[Lamp Intensity - LCSolution->Lamp Intensity - LCSolution (10450)]]</span> <div class="buttons"> <span class="btn-yes">[[Yes->Flow Cell Contamination (1426)]]</span> <span class="btn-no">[[No->Wavelength Accuracy Check(1440)]]</span><span class="btn-back"><<back "Back">></span> </div>

<div class="container"><h1 class="title">Making the Power Connection</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector/Power_SPD.jpg" alt="Power Cord"></div> <ul> <li>Turn the power switch on the detector OFF before plugging the instrument into the power supply.</li> <li>Insert the plug end of the power cable directly into the power supply outlet.</li> <li>Insert the connector end of the power cable into the power connector on the back of the detector.</li> </ul> <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>WARNING: The three-line type power cable supplied as an accessory includes the grounding wire. Connect this wire to ground to prevent electrical shock and to secure stable operation of the instrument.</strong> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Power Cord Damage</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector/PowerCordDamage.jpg" alt="Power Cord Damage"></div> <h3>Handle the power cord with care. Observe the following precautions to avoid the possibility of cord damage that may result in fire, electric shock or instrument malfunction.</h3> <ul> <li>Do not place heavy objects on the cord.</li> <li>Keep hot items away from the power cord.</li> <li>Do not modify the power cord in any way.</li> <li>Do not bend the cord excessively.</li> <li>To unplug the cord, pull on the plug itself, NOT on the cord.</li> <li>If the cord becomes damaged, replace it with a new one immediately.</li> </ul> <div class="ts-question">Is the power cord damaged?</div> <div class="buttons"> <span class="btn-yes">[[Yes->Power Cord Replacement (1401)]]</span> <span class="btn-no">[[No->Power Supply Requirements(1404)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Power Cord Replacement</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector/PowerCord.jpg" alt="Power Cord"></div> A power supply cable is supplied with each detector. If the cord becomes damaged, replace it with a new one immediately. Visit <a href="https://store.shimadzu.com">our web store</a> to order parts. <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div>

<div class="container"><h1 class="title">Power Supply Requirements</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector/PowerReq_M30.jpg" alt="Power Supply Requirements"></div> Verify that the power outlet has a sufficient capacity. If capacity is insufficient, a power outage or voltage drop can occur. Power drops will affect the performance of this instrument and any other instruments that are connected to the same power supply circuit. <div class="ts-question">Does the power supply meet the power supply requirements? </div> <div class="buttons"> <span class="btn-yes">[[Yes->Checking the Fuses(1409)]]</span> <span class="btn-no">[[No->Power Requirements(1407)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Power Requirements</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector/PowerReq_M30.jpg" alt="Power Cord"></div> Verify that the power outlet has a sufficient capacity. If capacity is insufficient, a power outage or voltage drop can occur. Power drops will affect the performance of this instrument and any other instruments that are connected to the same power supply circuit. <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Checking the Fuses</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector/Fuse_0.jpg" alt="Checking the Fuses"></div> <h3>If the circuit has been overloaded, the fuses may blow to protect the system electronics.</h3> <ol> <li>Open the fuse compartment cover with a screwdriver.</li> <li>Remove both of the fuse holders from the fuse compartment.</li> <li>Remove the old fuses from the fuse holders.</li> <li>Inspect the fuse by holding it up to the light. The glass should be clear and clean and the wire should be continuous and extend from one end of the fuse to the other. The fuse is blown and must be replaced if the glass is discolored with black or brown smudges or the wire is clearly broken or not visible at all.</li> <li>Insert the new fuses into the fuse holders.</li> <li>Reinstall the fuse holders</li> </ol> <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>WARNING: Turn the instrument OFF and disconnect the power supply before proceeding.</strong> <div class="ts-question">Have the fuses blown?</div> <div class="buttons"> <span class="btn-yes">[[Yes->Fuse Replacement - SPD-M30A(946)]]</span> <span class="btn-no"><a href="https://www.ssi.shimadzu.com/forms/tech-support/index.html?product_category=VirtualAdvisor&product_name=Nexera&page=webform_start">Contact Shimadzu</a></span><span class="btn-back"><<back "Back">></span></div> </div>

<div class="container"><h1 class="title">Fuse Replacement - SPD-M30A</h1> <div class="center"> <iframe width="900" height="506" src="https://www.youtube.com/embed/D9h_3rtxeR8?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <ol> <li>Open the fuse compartment cover with a screwdriver.</li> <li>Remove both of the fuse holders from the fuse compartment.</li> <li>Remove the old fuses from the fuse holders.</li> <li>Insert the new fuses into the fuse holders.</li> <li>Reinstall the fuse holders.</li> <li>Close the fuse compartment cover.</li> </ol> <div class="buttons"> <span class="btn-back"><<back "Back">></span></div> </div>

<div class="container"><h1 class="title">Wavelength Accuracy Check</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector/WavelengthAccuracy_0.jpg" alt="Wavelength Accuracy Check"></div> <h3>If the wavelength loses calibration, it will result in a loss of sensitivity.</h3> The wavelength accuracy check verifies that the difference between the set wavelength and the actual wavelength is within the check criteria. <div class="ts-question">Has there been a loss in sensitivity?</div> <span class="btn-link">[[Wavelength Accuracy Check->Wavelength Accuracy Check Video(10512)]]</span><br> <div class="buttons"> <span class="btn-yes">[[Yes->Wavelength Calibration Description(1443)]]</span> <span class="btn-no">[[No->Noise or Drift(1446)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Flow Cell Contamination</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector/FlowCellSPDM.jpg" alt="Flow Cell Contamination"></div> A dirty flow cell can cause baseline drift, instability, noise and other adverse affects to the chromatography. Depending on the type of samples that are routinely tested, a maintenance plan for the flow cell should be developed and adhered to for optimal flow cell performance. <div class="ts-question">Has the flow cell been cleaned recently?</div> <div class="buttons"> <span class="btn-yes">[[Yes->Mobile Phase Absorption (1429)]]</span> <span class="btn-no">[[No->Flow Cell Cleaning - SPD-M30A (952)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Flow Cell Cleaning - SPD-M30A</h1> <div class="center"> <iframe width="900" height="506" src="https://www.youtube.com/embed/uD3DNEeCRA4?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <ol> <li>Open the doors. </li> <li>Loosen the clamp securing the flow line couplings and disconnect the flow lines. </li> <li>Disconnect the heater assembly connector. </li> <li>Loosen the screws and remove the flow cell. </li> <li>Attach a syringe filled with 2-propanol to the flow cell inlet. </li> <li>Flush the flow cell with 2-propanol. Repeat with mobile phase. </li> <li>Reinsert the flow cell and finger tighten the screws. </li> <li>Reconnect the heater assembly connector. </li> <li>Reconnect the flow lines and secure the couplings in the clamp. </li> <li>Close the doors. </li> </ol> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Mobile Phase Absorption</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector/Bottle.jpg" alt="Mobile Phase Absorption"></div> The mobile phase and any impurities therein, will absorb a portion of the lamp energy. Always test the lamp energy with degassed, DI water in the flow cell. This will ensure a consistent measurement of the lamp energy. <div class="ts-question">Does the mobile phase have a high absorption?</div> <div class="buttons"> <span class="btn-yes">[[Yes->Priming the Pump(1431)]]</span> <span class="btn-no">[[No->Lamp Intensity Check(1432)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Wavelength Calibration Description</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector/PDAIntensity.jpg" alt="Wavelength Calibration Description"></div> The wavelength calibration procedure is performed when the results of the wavelength accuracy test do not meet the specifications, and after confirming that the light source spectrum shows no abnormality. Wavelength calibration determines the wavelength of light incident to each individual light receiving element in the photodiode array. A standard wavelength light is measured by each of several elements, and an element number per wavelength relationship is created and stored. The result of the wavelength calibration is stored in the detector unit, and the PC generates the relationship formula based on this result. Therefore, unless the light axis changes as a result of optical system adjustment, or the stored data is lost, there is no need to perform the wavelength calibration. <div class="buttons"> <span class="btn-link">[[Wavelength Calibration->Wavelength Calibration(10513)]]</span> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Noise or Drift</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector/NoiseDrift.jpg" alt="Noise/Drift"></div> <h3>NOISE: Baseline noise is the small variation of the baseline from a straight line.</h3> Noise can be caused by one or more of the following factors: *electric signal fluctuations *lamp instability *temperature fluctuations *other factors <h3>DRIFT: Baseline drift is the amount of baseline deviation from horizontal over a period of time. Drift is usually caused by temperature fluctuations and the first hour of operation after the detector lamp is turned on.</h3> <div class="ts-question">Is the baseline noisy or drifting?</div> <div class="buttons"> <span class="btn-yes">[[Noisy->Noise Pattern]]</span> <span class="btn-no">[[Drifting->Column Equilibration(1483)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Wavelength Calibration</h1> <div class="center"> <iframe width="900" height="506" src="https://www.youtube.com/embed/Vjuh0Ts49TE?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <ol> <li>Visually confirm that there are no leaks or bubbles in the flow line.</li> <li>From the main menu, select PDA Utility.</li> <li>Press the Wavelength Calibration button. The PDA Wavelength Calibration screen is displayed.</li> <li>Press the Calibrate button to perform the wavelength calibration.</li> <li>After calibration, perform the Wavelength Accuracy Check with D2/Ho to verify that the wavelength accuracy satisfies the specifications.</li> </ol> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Column Equilibration</h1> <div class="center"> <img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector/ColumnEquilibrium.jpg" alt="Wavelength Calibration Description"> </div> Column equilibration is the time required for the mobile phase and stationary phase of the column to stabilize. Typically, when the mobile phase first starts flowing through the column, the baseline will drift dramatically and begin to stabilize when equilibration is reached. The time required for column equilibration is dependant on the column dimensions. Typically, allowing 15-20 column volumes of mobile phase to flow through the column is necessary to achieve equilibration. The table above displays some average times required for column equilibration. <div class="ts-question">Has the column been allowed to equilibrate?</div> <div class="buttons"> <span class="btn-yes">[[Yes->Mobile Phase Contamination(1487)]]</span> <span class="btn-no">[[No->Longer Column Equilibration(1485)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Noise Pattern</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector/Sawtooth.jpg" alt="Noise Pattern"></div> If the noise in the baseline has a pattern, it is an indication that the problem may be related to the pump. If the noise in the baseline does not have a cyclical pattern, it is an indication that the problem may be related to factors other than the pump. <div class="ts-question">Does the noise have a noticeable pattern?</div> <div class="buttons"> <span class="btn-yes">[[Yes->Pump Power and Flow Rate]]</span> <span class="btn-no">[[No->Column Insulation (1452)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Pump Power and Flow Rate</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector/PowerSwitch.jpg" alt="Pump Power and Flow Rate"></div> <h3>Turn the pump OFF.</h3> <ol> <li>The power switch is located on the front of the instrument.</li> <li>Press the PUMP key on the pump keypad to start and stop the pumping action.</li> <li>Once the power is on and the pump is pumping, the flow rate must be properly set.</li> </ol> <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>Press the power switch once to turn the pump ON and again to turn the pump OFF. <br><br>It is possible for power to the pump to be ON while the pumping action is OFF.<br> The flow rate can be set at the pump (local mode) or via the controller or data system software (remote mode).</strong> <div class="ts-question">Do the spikes stop when the pump is turned OFF?</div> <div class="buttons"> <span class="btn-yes"><a href="/service-support/virtual-advisor/nexera/advanced-troubleshooting/troubleshooting-the-pump/index.html">Troubleshooting The Pump</a></span> <span class="btn-no">[[No->Vibration (1466)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Column Insulation</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector/ColumnTemp.jpg" alt="Noise Pattern"></div> <h3>A column oven can be used to isolate the column and flow lines from the ambient temperature fluctuations.</h3> Maintaining the column temperature at a constant level increases analysis reproducibility and separation performance. There are three different types of thermal insulation devices available for the LC column. *CTO-30A and the CTO-30AS with the SIL-30AC MP Column Oven - Uses a heating block to control the temperature of the LC column. *CTO-20A/20AC Column Oven - Uses an air-circulated thermostatic chamber to maintain the temperature of the LC column and flow lines. *Jacket-type Devices - Uses plumbing insulation or heat tape to a lesser degree of effectiveness. <div class="ts-question">Is the instrument exposed to ambient drafts or air currents?</div> <div class="buttons"> <span class="btn-yes">[[Yes->Column Oven (1495 / 1497)]]</span> <span class="btn-no">[[No->Vibration (1455)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Vibration</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector/Vibration.jpg" alt="Vibration"></div> The optical components of the SPD-M30A photodiode array detector are very sensitive. Excessive vibration in the installation site may manifest in the chromatogram as non-cyclical noise in the baseline. Ambient vibration may have a variety of sources. Most often, the vibration comes from other motors in the direct vicinity of the detector. <div class="ts-question">Is the instrument exposed to excessive vibrations?</div> <div class="buttons"> <span class="btn-yes">[[Yes->Installation Site Requirements (1457)]]</span> <span class="btn-no">[[No->Bubbles in the Flow Cell (1459)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Installation Site Requirements</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector/Install_a.jpg" alt="Installation Site Requirements"></div> When choosing the installation site for the HPLC system, consider the weight of all of the system components. The lab table on which the instrument is installed should be sufficiently strong enough to support the weight of the entire system. The location should be level and stable with a depth of at least 600mm. Avoid installation sites that are exposed to corrosive gases and excessive dust. Avoid installation sites that are exposed to excessive vibrations or temperature fluctuations. <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Electronic Interference</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector/ElecNoise.jpg" alt="Electronic Interference"></div> Electronic noise interference is noticeable in the baseline as erratic noise in the baseline that remains after the pump is turned off. This noise can be caused by other electronic devices that are connected to the same electronic circuit. Often, these other devices have some type of motor that causes the interference. <div class="ts-question">Is the detector performance being affected by electronic interference?</div> <div class="buttons"> <span class="btn-yes">[[Yes->Eliminate Electronic Interference (1471)]]</span> <span class="btn-no">[[No->Lamp Intensity Check (1472)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Eliminate Electronic Interference</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector/ElecNoise.jpg" alt="Eliminate Electronic Interference"></div> Electronic noise interference is noticeable in the baseline as erratic noise in the baseline that remains after the pump is turned off. This noise can be caused by other electronic devices that are connected to the same electronic circuit. Often, these other devices have some type of motor that causes the interference. Review the electronic circuit and remove the other devices one at a time to determine the source of the interference. Another way to verify that there is no electronic interference is to have dedicated circuits for each HPLC system. <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Lamp Intensity Check - LCsolution</h1> <div class="center"> <iframe width="900" height="506" src="https://www.youtube.com/embed/rv4XK8kIcF8?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> *D2 - maximum intensity at or around 235 nm ≥ 7V *Record the lamp intensities for future reference. <br> <ol> <li>Use the supplied syringe and needle to fill the flow cell with degassed DI (deionized) water.</li> <li>Select START - PROGRAMS - LABSOLUTIONS - LCSOLUTION - LC MAINTENANCE TOOLS.</li> <li>The LC Tool Launcher is displayed. Select 'PDA Utility' and 'Instrument 1' and then press the Run button.</li> <li>The Login screen is displayed. Enter the User ID and Password and then press the OK button.</li> <li>The default UserID/password are as follows: UserID: admin Password: (none)</li> <li>The PDA Utility screen is displayed. Press the Wavelength Calibration button.</li> <li>The PDA Wavelength Calibration screen is displayed. Select Function - Check Light F7..</li> <li>The Adjust Light screen is displayed. Select either the D2 or the W lamp.</li> <li>Enter the wavelength used for the Intensity check..</li> <li>Enter the threshold to be used for the acceptance criterion. Press the OK button.</li> <li>The PDA Wavelength Calibration screen is displayed.</li> <li>Confirm that the voltage at the selected wavelength is larger than the set threshold.</li> </ol> <div class="buttons"> <span class="btn-back"><<back "Back">></span></div> </div>

<div class="container"><h1 class="title">Mobile Phase Contamination</h1> <div class="center"> <img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector/Bottle.jpg" alt="Mobile Phase Bottle"> </div> The mobile phase may contain impurities that affect the chromatography. These impurities may arise from a number of sources. These sources may include: *using a low grade purity solvent. *allowing bacterial growth in the solution. *precipitation of buffers from the solution. <div class="ts-question">Does the mobile phase contain impurities?</div> <div class="buttons"> <span class="btn-yes">[[Yes->Eliminating Mobile Phase Contamination (1489)]]</span> <span class="btn-no">[[No->Turning the Lamp Power On and Off(1491)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Longer Column Equilibration</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector/ColumnEquilibrium.jpg" alt="Column Equilibrium"></div> The time required for column equilibration is dependent on the column dimensions. Typically, flushing 15-20 column volumes through the column is necessary to achieve equilibration. Sometime the column may become 'contaminated' with sample matrix etc. On these occasions, it may be necessary to flush the column for a longer period than the average recommendations. Use the table above to determine the average time required for column equilibration. <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Eliminating Mobile Phase Contamination</h1> <div class="center"> <img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector/Bottle.jpg" alt="Bottle"> </div> The mobile phase may contain impurities that affect the chromatography. These impurities may arise from a number of sources. There are a number of precautions to avoid mobile phase contamination: *Use HPLC grade solvents or better. *Prepare mobile phase fresh daily to avoid microbial growth. *Filter and degas all mobile phase components. <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Turning the Lamp Power On and Off</h1> <div class="center"> <img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector//PowerIndicator_a.jpg" alt="Power Indicator"> </div> <h3>It takes some time for the baseline to stabilize after the lamp has been turned ON.</h3> After the instrument power switch is turned ON, the status indicator is illuminated with a green color. As the deuterium (D2) and tungsten (W) lamp LEDs are turned on, the status indicator light turns red. In this case, the red status indicator does NOT signify an error. The status indicator turns green again and the D2 and W lamp LEDs begin blinking alternately. During this process, the motor home position detection and slit initialization operations are conducted. When these operations are complete, the lamp LEDs blink more quickly to indicate that the light source lamps are being illuminated. The lamp LEDs stop blinking when the light source lamps are fully illuminated. <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>NOTE: The setting that determines which lamp will be illuminated is retained from the conditions that were in place at the time of instrument shutdown.</strong> <div class="ts-question">Has the lamp been turned ON long enough for the baseline to stabilize?</div> <div class="buttons"> <span class="btn-yes">[[Yes->Ambient Temperature(1494)]]</span> <span class="btn-no">[[No->Lamp Equilibration (1493)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Ambient Temperature</h1> <div class="center"> <img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector/ColumnTemp.jpg" alt="Ambient Temperature"> </div> <h3>Maintaining the column temperature at a constant level increases analysis reproducibility and separation performance.</h3> There are three different types of thermal insulation devices available for the LC column. *CTO-30A and the CTO-30AS with the SIL-30AC MP Column Oven - Uses a heating block to control the temperature of the LC column. *CTO-20A/20AC Column Oven - Uses an air-circulated thermostatic chamber to maintain the temperature of the LC column and flow lines. *Jacket-type Devices - Uses plumbing insulation or heat tape to a lesser degree of effectiveness. <div class="ts-question">Is the ambient temperature stable?</div> <div class="buttons"> <span class="btn-yes">[[Yes->Column Insulation(1496)]]</span> <span class="btn-no">[[No->Column Oven (1495 / 1497)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Lamp Equilibration</h1> <div class="center"> <img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector/LampOnOff.jpg" alt="Lamp Power"> </div> <h3>After the lamps are turned ON, there are a number of factors that can affect how fast the baseline will stabilize.</h3> These factors include: *ambient temperature *age of the lamp *cleanliness of the detector Allow the lamp to equilibrate until the baseline is stable enough for the analysis. 1.5 hours is usually a sufficient amount of time. <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Column Oven</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector/CTO_Front.jpg" alt="Column Oven"></div> The Shimadzu column ovens (CTO-30A/30AS) use a heating block to control the temperature of the LC column to improve retention time reproducibility and separation performance. The Shimadzu column ovens (CTO-20A/20AC) are air-circulated thermostatic chambers designed to improve retention time reproducibility and separation performance by maintaining the temperature of the LC column and flow lines. In addition to the column, the oven can also house manual injectors, solvent mixers, column switching valves and other flow line accessories. Installing these items into the column oven isolates them from the influence of ambient temperature fluctuations and allows more stable analysis. <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Column Insulation</h1> <div class="center"> <img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector/ColumnTemp.jpg" alt="Ambient Temperature"></div> <h3>A column oven can be used to isolate the column and flow lines from the ambient temperature fluctuations.</h3> Maintaining the column temperature at a constant level increases analysis reproducibility and separation performance. There are three different types of thermal insulation devices available for the LC column. *CTO-30A and the CTO-30AS with the SIL-30AC MP Column Oven - Uses a heating block to control the temperature of the LC column. *CTO-20A/20AC Column Oven - Uses an air-circulated thermostatic chamber to maintain the temperature of the LC column and flow lines. *Jacket-type Devices - Uses plumbing insulation or heat tape to a lesser degree of effectiveness. <div class="ts-question">Is the instrument exposed to ambient drafts or air currents?</div> <div class="buttons"> <span class="btn-yes">[[Yes->Column Oven (1495 / 1497)]]</span> <span class="btn-no">[[No->Flow Line Leaks (1498)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Flow Line Leaks</h1> <div class="center"> <img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector/FlowLine.jpg" alt="Flow Line Leaks"> </div> A leak in the flow line before the detector flow cell could explain the absence of sample peaks. Visually inspect the flow line from needle to flow cell looking for signs of a leak: *Puddles *Drips *Crystallization of buffer <div class="ts-question">Are the connections to the inlet or outlet tubing of the flow cell leaking?</div> <div class="buttons"> <span class="btn-yes">[[Yes->Tubing-Flow Line Connections (1954)]]</span> <span class="btn-no"><a href="https://www.ssi.shimadzu.com/forms/techsupport/index.html?product_category=VirtualAdvisor&product_name=Nexera&page=webform_start">Contact Shimadzu</a></span> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Tubing - Flow Line Connections</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector/Tubing_FlowLineConnections.jpg" alt="Tubing Flow Line Connections"></div> <ol> <li>Place a male nut and ferrule on the tubing.</li> <li>Insert the end of the tubing with the ferrule completely into the appropriate fitting.</li> <li>Push the tubing into the fitting until it butts up against the end of the fitting (tubing stop position).</li> <li>While making sure the tubing remains in the tubing stop position, securely tighten the male nut. The ferrule is secured to the tubing.</li> </ol> <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>NOTE: It is important to apply a significant amount of pressure to the tubing while tightening the male nut.</strong><br><br> <span class="btn-link">[[Cutting Resin->Cutting Resin (1951)]]</span><br> <span class="btn-link">[[Cutting SS->Cutting SS (1953)]]</span><br> <span class="btn-link">[[Tighten SS->Tighten SS (1952)]]</span><br> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Tubing - Cutting Resin</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector/TubingKnife.jpg" alt="Tubing-Cutting Resin"></div> When using PEEK tubing or equivalent resin tubing, use a straight edge razor knife to cut the tubing. To avoid the creation of void volumes, verify that the cut edges are clean right-angled cuts. <span class="btn-link">[[Tubing-Flow Line Connections->Tubing-Flow Line Connections (1954)]]</span><br> <span class="btn-link">[[Tighten SS->Tighten SS (1952)]]</span><br> <span class="btn-link">[[Cutting SS->Cutting SS (1953)]]</span><br> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Tubing - Cutting Cutting SS</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector/TubingSS.jpg" alt="Tubing - Cutting Cutting SS"></div> When using HPLC stainless steel tubing, it is best to use precut stainless steel tubing or employ a cutting technique that produces a smooth and even surface to the cut edge. If the cut edge of the tubing is not smooth and square, a void volume is created in the connection. <span class="btn-link">[[Tubing-Flow Line Connections->Tubing-Flow Line Connections (1954)]]</span><br> <span class="btn-link">[[Tighten SS->Tighten SS (1952)]]</span><br> <span class="btn-link">[[Cutting Resin->Cutting Resin (1951)]]</span><br> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Tubing - Tighten SS</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector/TubingTighten.jpg" alt="Tubing - Tighten SS"></div> Verify that the stainless steel unions are securely connected. Use two wrenches to tighten stainless steel couplings. <span class="btn-link">[[Tubing-Flow Line Connections->Tubing-Flow Line Connections (1954)]]</span><br> <span class="btn-link">[[Cutting Resin->Cutting Resin (1951)]]</span><br> <span class="btn-link">[[Cutting SS->Cutting SS (1953)]]</span><br> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Vibration</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector/Vibration.jpg" alt="Pump Power and Flow Rate"></div> The optical components of the SPD-M30A photodiode array detector are very sensitive. Excessive vibration in the installation site may manifest in the chromatogram as non-cyclical noise in the baseline. Ambient vibration may have a variety of sources. Most often, the vibration comes from other motors in the direct vicinity of the detector. <div class="ts-question">Is the instrument exposed to excessive vibrations that may be affecting instrument performance?</div> <div class="buttons"> <span class="btn-yes">[[Yes->Installation Site Requirements (1457)]]</span> <span class="btn-no">[[No->Electronic Interference (1469)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Bubbles in the Flow Cell</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector/SawtoothBaseline.jpg" alt="Bubbles in the Flow Cell"></div> Generally, the more dissolved air that is removed from the mobile phase the better the entire HPLC system will perform. However, when mobile phase does contain dissolved gas, the gas can escape from solution to form a small bubble in the flow line. These small bubbles can attach to any dirt particles that may be in the flow cell. As more gas escapes from solution, the bubble becomes larger causing a disturbance to the baseline. When the bubble gets large enough to flow out with the mobile phase, the baseline returns to zero. This phenomenon usually causes a very regular sinusoidal (sawtooth) shape to the baseline. It is also possible that the bubbles will manifest themselves as spiking vertical lines in the baseline <div class="ts-question">Are bubbles present in the flow cell?</div> <div class="buttons"> <span class="btn-yes">[[Yes->Back Pressure Tubing (1463)]]</span> <span class="btn-no">[[No->Flow Cell Contamination (1464/1480)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Back Pressure Tubing</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector/LC_BackPress_1.jpg" alt="Back Pressure Tubing"></div> Shimadzu detectors are all supplied with a 2-meter length of tubing to be used as a back-pressure device. One function of this tubing is to apply back pressure to the flow cell outlet to prevent the generation of air bubbles in the flow cell. <ol> <li>Attach a male PEEK nut to one of the ends of the supplied 2m cell outlet tubing.</li> <li>Screw the male PEEK nut attached to the tubing into the outlet coupling (union).</li> <li>Insert the other end of the tubing into the waste container so that the outlet end is pointing down but above the surface of the liquid in the container.</li> </ol> <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>CAUTION: Do not shorten the cell outlet tubing from the original 2m length.</strong> <br><br><br> <span class="btn-link">[[Mobile Phase Degassing->Mobile Phase Degassing (1462)]]</span><br> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Flow Cell Contamination</h1> <div class="center"> <img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector/FlowCellSPDM.jpg" alt="Flow Cell Contamination"> </div> <h3>Contamination or air bubbles in the flow cell may cause excessive noise in the baseline.</h3> A dirty flow cell can cause baseline drift, instability, noise and other adverse affects to the chromatography. Depending on the type of samples that are routinely tested, a maintenance plan for the flow cell should be developed and adhered to for optimal flow cell performance. <div class="ts-question">Has the inside of the flow cell been flushed?</div> <div class="buttons"> <span class="btn-yes"><a href="https://www.ssi.shimadzu.com/forms/tech-support/index.html?product_category=VirtualAdvisor&product_name=Nexera&page=webform_start">Contact Shimadzu</a></span> <span class="btn-no">[[No->Flow Cell Cleaning - SPD-M30A (952)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Priming the Pump</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector/Purge.jpg" alt="Priming the Pump"></div> <ol> <li>Fill the mobile phase reservoir with degassed mobile phase. </li> <li>Place the suction filter in the reservoir.</li> <li>Place the end of the drain tubing into the waste container, which has been placed on the floor.</li> <li>Turn the drain valve knob 90° counterclockwise to open the drain valve.</li> <li>Press CE to return to the Initial screen.</li> <li>Verify that the pressure for the displayed valve is in the range of [-0.3 to 0.3 MPa].</li> <li>Press the PURGE key. Pumping begins at the preset flow rate (P-FLOW).</li> <li>Allow the purge to run until the mobile phase flows from the drain tubing in a continuous and air free stream.</li> <li>Press the PURGE key to stop the pump.</li> </ol> <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>NOTE: Air bubbles in the drain tubing attached to the drain valve are normal and not a cause for concern. NOTE: If no mobile phase comes from the drain tubing, insert the tip of the supplied needle into the end of the drain tubing and using an attached syringe, draw the mobile phase through the tubing.</strong> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Wavelength Accuracy Check</h1> <div class="center"><iframe width="900" height="506" src="https://www.youtube.com/embed/STLIT_CyYP8?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <ol> <li>Fill the flow cell with degassed DI water and confirm that ther are not any air bubbles present.</li> <li> Select PDA Utility from the main menu. Press the Wavelength Check button.</li> <li>The PDA Wavelength Check screen is displayed.</li> <li>From the Wavelength Check menu, select D2/Ho.</li> <li>Wavelength accuracy is automatically evaluated and the result is displayed on the screen.</li> <li>From the Wavelength Check menu, select Hg.</li> <li> After the dark measurement is complete, a message appears instructing you to turn on the Hg lamp.</li> <li>Detach the flow cell and install the Hg jig. Turn ON the power to the jig.</li> <li>Press OK and follow the on-screen messages by turning the power to the jig ON and OFF accordingly.</li> <li> When the wavelength accuracy check is complete, the results are displayed on the screen.</li> </ol> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Mobile Phase Degassing</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector/Degas.jpg" alt="Mobile Phase Degassing"></div> Bubbles are caused by dissolved gases. These bubbles can be responsible for pump malfunction and baseline noise. To prevent the formation of bubbles in the pump head and flow cell, a degassing method must be included in the mobile phase preparation. Some typical methods include vacuum filtration, vacuum sonication and helium sparging. In addition to conducting degassing during mobile phase preparation, it is also recommended that an on-line degassing unit be included in the system. The Shimadzu on-line degasser (DGU-20A3/20A5) provides continuous mobile phase degassing without changing the composition of the mobile phase. <span class="btn-link">[[Back Pressure Tubing->Back Pressure Tubing (1463)]]</span><br> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Lamp Intensity Check</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector/LampIntensityCheckLCSolution.jpg" alt="Lamp Intensity Check"></div> !!!The lamps are consumable and eventually become too weak to be effective. Record the lamp intensities for future reference. <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>D2 - maximum intensity at or around 235 nm ≥ 7V<br> W - maximum intensity at or around 550-600 nm ≥ 7V</strong> <div class="ts-question">Has the lamp energy been depleted?</div> <span class="btn-link">[[Lamp Intensity - LCSolution->Lamp Intensity - LCSolution (10450)]]</span> <div class="buttons"> <span class="btn-yes">[[Yes->Deuterium Lamp - SPD-M30A (950)]]</span> <span class="btn-no">[[No->Bubbles in the Flow Cell (1477)]]</span><span class="btn-back"><<back "Back">></span> </div>

<div class="container"><h1 class="title">Bubbles in the Flow Cell</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector/SawtoothBaseline.jpg" alt="Bubbles in the Flow Cell"></div> Generally, the more dissolved air that is removed from the mobile phase the better the entire HPLC system will perform. However, when mobile phase does contain dissolved gas, the gas can escape from solution to form a small bubble in the flow line. These small bubbles can attach to any dirt particles that may be in the flow cell. As more gas escapes from solution, the bubble becomes larger causing a disturbance to the baseline. When the bubble gets large enough to flow out with the mobile phase, the baseline returns to zero. This phenomenon usually causes a very regular sinusoidal (sawtooth) shape to the baseline. It is also possible that the bubbles will manifest themselves as spiking vertical lines in the baseline <div class="ts-question">Are bubbles present in the flow cell?</div> <div class="buttons"> <span class="btn-yes">[[Back Pressure Tubing->Back Pressure Tubing (1463)]]</span> <span class="btn-no">[[No->Flow Cell Contamination (1464/1480)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Deuterium Lamp - SPD-M30A</h1> <div class="center"> <iframe width="900" height="506" src="https://www.youtube.com/embed/leuCJwCon-k?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>WARNING: Turn OFF the power to the instrument and unplug the power cable before proceeding.</strong> <br><br> <ol> <li>Open the doors.</li> <li>Remove the lamp cover.</li> <li>Disconnect the deuterium lamp connector.</li> <li>Use a screwdriver to loosen the lamp retaining screws</li> <li>Remove the old lamp from the instrument.</li> <li>Install the new D2 lamp assembly.</li> <li>Secure the lamp retaining screws.</li> <li>Reconnect the lamp connector.</li> <li>Re-install the lamp cover.</li> <li>Close the doors.</li> </ol> <div class="buttons"> <span class="btn-back"><<back "Back">></span></div> </div>

<div class="container"><h1 class="title">Lamp Intensity Check</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector/LampIntensityCheckLCSolution.jpg" alt="Lamp Intensity Check"></div> !!!The lamps are consumable and eventually become too weak to be effective. Record the lamp intensities for future reference. <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>D2 - maximum intensity at or around 235 nm ≥ 7V<br> W - maximum intensity at or around 550-600 nm ≥ 7V</strong> <div class="ts-question">Has the lamp energy been depleted?</div> <span class="btn-link">[[Lamp Intensity - LCSolution->Lamp Intensity - LCSolution (10450)]]</span> <div class="buttons"> <span class="btn-yes">[[Yes->Deuterium Lamp - SPD-M30A (950)]]</span> <span class="btn-no">[[No->PDA Exposure Time(1436)]]</span><span class="btn-back"><<back "Back">></span> </div>

<div class="container"><h1 class="title">PDA Exposure Time</h1> <div class="center"><img src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/nexera/troubleshooting-pda-detector/PDAScan.jpg" alt="Power Cord"></div> Array detectors allow the user to record the full UV-VIS spectra of samples that pass through the flow cell. The SPD-M30A photodiode array detector (PDA) has 512 discrete photodiodes arranged at the image plane to allow for simultaneous detection of a range of wavelengths. The lamp provides the photometric energy that is used to excite the photodiodes.The lamp energy is directed to the individual photodiodes using a grating. The exposure time is the amount of time that is required to provide enough energy to excite each photodiode. When the lamp energy is high, as in a new lamp, the exposure time can be automatically set to a lower setting. As the lamp energy becomes depleted with use, the exposure time can be automatically adjusted to provide optimal performance from a weaker lamp. <div class="buttons"><span class="btn-yes"><a href="https://www.ssi.shimadzu.com/forms/tech-support/index.html?product_category=VirtualAdvisor&product_name=Nexera&page=webform_start">Contact Shimadzu</a></span> <span class="btn-no">[[No->Setting the Exposure Time(10511)]]</span><span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Setting the Exposure Time</h1> <div class="center"> <iframe width="900" height="506" src="https://www.youtube.com/embed/O2kcOcE7uXA?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <ol> <li>Flush the flow cell with degassed DI water to remove any bubbles from the flow cell.</li> <li>Select PDA Utility from the main menu. Press the Wavelength Calibration button.</li> <li>The PDA Wavelength Calibration screen is displayed.</li> <li>Press the Exposure Time button.</li> <li>The exposure time setting is performed and the set value is displayed.</li> <li>Confirm the set value and press the Yes button.</li> <li>Perform the Lamp Intensity Check.</li> </ol> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>