i-Series Pump Periodic Maintenance

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<div class="container"><h1 class="title">Pump Periodic Maintenance</h1> <h3>Select a periodic maintenance procedure below to learn more.</h3> <span class="btn-link">[[Attaching the Pump Head - LC-2040->Attaching the Pump Head - LC-2040 (1219)]]</span> <span class="btn-link">[[Attaching the Pump Head - LC-2030->Attaching the Pump Head - LC-2030 (314)]]</span> <span class="btn-link">[[Check the Diaphragm Assembly->Check the Diaphragm Assembly (323)]]</span> <span class="btn-link">[[Cleaning and Replacing the Plunger and Diaphragm->Cleaning and Replacing the Plunger and Diaphragm (320)]]</span> <span class="btn-link">[[Cleaning of the Automatic Rinsing Kit->Cleaning of the Automatic Rinsing Kit (333)]]</span> <span class="btn-link">[[Inspecting the Check Valve->Inspecting the Check Valve (326)]]</span> <span class="btn-link">[[Inspecting the Plunger and Diaphragm->Inspecting the Plunger and Diaphragm (319)]]</span> <span class="btn-link">[[Inspecting/Cleaning/Replacing the Suction Filter - Pump->Inspecting/Cleaning/Replacing the Suction Filter - Pump (1198)]]</span> <span class="btn-link">[[Performing an Ultrasonic Cleaning of the Check Valve->Performing an Ultrasonic Cleaning of the Check Valve (325)]]</span> <span class="btn-link">[[Pump Pressure Zero Adjustment->Pump Pressure Zero Adjustment (359)]]</span> <span class="btn-link">[[Removing the Pump Head - LC-2040->Removing the Pump Head - LC-2040 (1220)]]</span> <span class="btn-link">[[Removing the Pump Head - LC-2030->Removing the Pump Head - LC-2030 (313)]]</span> <span class="btn-link">[[Replacing the Drain Valve ASSY->Replacing the Drain Valve ASSY (332)]]</span> <span class="btn-link">[[Replacing the Line Filter->Replacing the Line Filter (330)]]</span> <span class="btn-link">[[Replacing the Plunger and Diaphragm->Replacing the Plunger and Diaphragm (321)]]</span> <span class="btn-link">[[Replacing the Plunger Seal - Part 1 - LC-2040->Replacing the Plunger Seal - Part 1 - LC-2040 (1217)]]</span> <span class="btn-link">[[Replacing the Plunger Seal - Part 2 - LC-2040->Replacing the Plunger Seal - Part 2 - LC-2040 (1142)]]</span> <span class="btn-link">[[Replacing the Plunger Seal - Part 1 - LC-2030->Replacing the Plunger Seal - Part 1 - LC-2030 (322)]]</span> <span class="btn-link">[[Replacing the Plunger Seal - Part 2 - LC-2030->Replacing the Plunger Seal - Part 2 - LC-2030 (1218)]]</span> <span class="btn-link">[[Rinsing the Check Valve->Rinsing the Check Valve (324)]]</span> </div>

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<div class="container"><h1 class="title">Attaching the Pump Head - LC-2040</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/heP8sj6_T24?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Warnings</h3> <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>Gently insert the pump head horizontally along the plunger. The plunger may be damaged if the pump head is forcefully inserted.</strong> <h3>Procedures</h3><ol> <li>With the arrow on the pump head (a) pointing up, gradually tighten the 2 hexagonal socket screws (A) using an Allen wrench (M5)</li> <li>By alternating between the left and right screw, and attach the pump head (B).</li> <li>Tighten the male nut PEEK (A) of the inlet tube to the inlet check valve (B) by hand.</li> <li>Tighten the male nut (A) of the SUS pipe R (B) pressure sensor with an 8 mm wrench and attach SUS pipe R (B).</li> <li>While securing the outlet check valve (A) with a 10 mm wrench (B), tighten the male nut (C) of the SUS pipe R outlet check valve with an 8 mm wrench (D).</li> <li>Attach the original rinsing kit cap (B) to the rinse solution bottle (A)</li> <li>Remove the outlet FEP tubing (E) (upward U-shape) from the rinse solution bottle</li> <li>Attach the syringe needle (C) to the end of the FEP tubing (E)(upward U-shape)</li> <li>Use the syringe (D) to suction out the 10% 2-propanol solution from the rinse flow line</li> <li>Return the rinsing kit to the reservoir tray.</li> <li>Close the pump door.</li> </ol> <h3>Related Notes</h3>Tighten the left and right hexagonal socket screws 90° at a time, and then firmly tighten using the long side of the Allen wrench. <h3>Necessary Tools</h3><ul> <li>10mm Wrench</li> <li>8mm Wrench</li> <li>M5 Allen Wrench</li> <li>Syringe</li> <li>Syringe Needle</li> </ul> <h3>Related Procedures</h3><span class="btn-link">[[Removing the Pump Head->Removing the Pump Head - LC-2040 (1220)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Check the Diaphragm Assembly</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/XefsMGHjVzk?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Procedures</h3>If you have not already done so, please see [[Replacing the Plunger and Diaphragm->Replacing the Plunger and Diaphragm (321)]] before viewing this video <ol><li>Insert the syringe needle (B) into the end of the accessory syringe (A).</li> <li>Attach the accessory flangeless fitting ASSY (B) to the end of the syringe needle (A)</li> <li>Rotate it clockwise to attach to the rinse fluid outlet (a) at the top of the head holder.</li> <li>Cover the rinse fluid inlet (a) at the bottom of the head holder with a finger.</li> <li>Press and release the plunger of the syringe. If there is no air leak, the plunger of the syringe will return to its original position.</li> <li>Loosen the fitting (A) at the top of the head holder and remove the syringe.</li> <li>Loosen the head holder top and bottom fittings (A, B), and attach the original rinse tube.</li> <li>Reattach the SUS tubing and the inlet tube.</li> <li>Tighten the male nut PEEK (A) of the inlet tube to the inlet check valve (B) by hand.</li> <li>Tighten the male nut (A) of the SUS pipe R (B) pressure sensor with an 8 mm wrench, and attach SUS pipe R (B)</li> <li>While securing the outlet check valve(A) with a 10 mm wrench (B), tighten the male nut (C) of the SUS pipe R outlet check valve with an 8 mm wrench (D).</li> <li>Close the pump door.</li> <li>Press [Next]. The next screen is displayed.</li> <li>Select the mobile phase that will be used for purging, and then press [Next]. The next screen is displayed.</li> <li>Press [Start]. Purging starts. After purging finishes, the next screen is displayed automatically.</li> <li>After replacing the plunger seal, perform a check.</li> <li>Is the pumping (pressure) stable?</li> <li>Is fluid leaking from the gap between the pump head and the head holder?</li> <li>Press [Exit]. The replacement history is recorded in the maintenance log.</li> </ol> <h3>Related Notes</h3>If there is an air leak, check the connection portion between the flangeless fitting ASSY and the head holder. If there is no problem, disassemble it again to check the attachment of the diaphragm. <h3>Necessary Tools</h3><ul> <li>10mm Wrench</li> <li>8mm Wrench</li> </ul> <h3>Related Procedures</h3><span class="btn-link">[[Attaching the Pump Head->Attaching the Pump Head - LC-2030 (314)]] </span> <span class="btn-link">[[Cleaning and Replacing the Plunger and Diaphragm->Cleaning and Replacing the Plunger and Diaphragm (320)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Replacing the Plunger and Diaphragm</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/VqnViQSsBxk?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Warnings</h3> <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>Do NOT allow foreign particles or crystals into the head holder. Foreign particles from the mobile phase solution or crystals from the buffer solution getting into the head holder may cause scratches on the surface of the plunger.</strong> <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>Gently push in the head holder horizontally along the plunger. The plunger may become damaged if the head holder is forcefully inserted.</strong> <h3>Procedures</h3>If you have not already done so, please view [[Cleaning and Replacing the Plunger and Diaphragm->Cleaning and Replacing the Plunger and Diaphragm (320)]] before viewing this video<ol> <li>Press “Stop.”</li> <li>Replace the Plunger Seal (Manual Steps 1-9)</li> <li>Press [Stop] after the usage value is reset.</li> <li>Press [Plunger&Diaphragm] on the [Replacement Parts Selection] screen.</li> <li>Press [Right], and then press [Next].</li> <li>Press [Next]. The next screen is displayed.</li> <li>Attach the head holder and the pump head.</li> <li>Gradually tighten the 2 hexagonal socket screws (B) using an Allen wrench (M4) by alternating between the left and right screw, and attach the head holder (A).</li> <li>Grip the end of the plunger with two fingers and adjust the gap (C) between the plunger (A) and the hole in the head holder (B) so that it is uniform.</li> <li>Wipe off the plunger surface with clean wiping paper soaked in 2-propanol, being careful not to tilt the plunger.</li> <li>Move the plunger gently. Moving it too abruptly could scratch the plunger surface or cause it to break off.</li> <li>Attach the head holder. Tighten the left and right hexagonal socket screws 90 ° at a time, and then firmly tighten using the long side of the Allen wrench.</li> <li>Press [Next]. The next screen is displayed.</li> <li>Select the replaced part and press [Next]. The next screen is displayed.</li> <li>Proceed to [[Check the Diaphragm Assembly->Check the Diaphragm Assembly (323)]]</li> </ol> <h3>Related Notes</h3><ul> <li>If part of the seal remains attached, airtightness cannot be maintained.</li> <li>Tighten the left and right hexagonal socket screws 90 ° at a time, and then firmly tighten using the long side of the Allen wrench.</li> </ul> <h3>Necessary Parts</h3>Plunger Holder ASSY Maintenance Part 228-35281-97 Qty: 1 Remarks: 95 Gasket (Set of 2) Be sure to replace when disassembling and cleaning the flow cell. *1 <h3>Necessary Tools</h3>M4 Allen Wrench <h3>Related Procedures</h3> <span class="btn-link">[[Cleaning and Replacing the Plunger and Diaphragm->Cleaning and Replacing the Plunger and Diaphragm (320)]]</span> <span class="btn-link">[[Check the Diaphragm Assembly->Check the Diaphragm Assembly (323)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Attaching the Pump Head - LC-2030</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/UUlDeCKDlxM?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Warnings</h3> <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>Gently insert the pump head horizontally along the plunger. The plunger may be damaged if the pump head is forcefully inserted.</strong> <h3>Procedures</h3><ol> <li>With the arrow on the pump head (a) pointing up, gradually tighten the 2 hexagonal socket screws (A) using an Allen wrench (M5)</li> <li>By alternating between the left and right screw, and attach the pump head (B).</li> <li>Tighten the male nut PEEK (A) of the inlet tube to the inlet check valve (B) by hand.</li> <li>Tighten the male nut (A) of the SUS pipe R (B) pressure sensor with an 8 mm wrench and attach SUS pipe R (B).</li> <li>While securing the outlet check valve (A) with a 10 mm wrench (B), tighten the male nut (C) of the SUS pipe R outlet check valve with an 8 mm wrench (D).</li> <li>Attach the original rinsing kit cap (B) to the rinse solution bottle (A)</li> <li>Remove the outlet FEP tubing (E) (upward U-shape) from the rinse solution bottle.</li> <li>Attach the syringe needle (C) to the end of the FEP tubing (E)(upward U-shape)</li> <li>Use the syringe (D) to suction out the 10% 2-propanol solution from the rinse flow line</li> <li>Return the rinsing kit to the reservoir tray.</li> <li>Close the pump door.</li> </ol> <h3>Related Notes</h3>Tighten the left and right hexagonal socket screws 90° at a time, and then firmly tighten using the long side of the Allen wrench. <h3>Necessary Tools</h3><ul> <li>10mm Wrench</li> <li>8mm Wrench</li> <li>M5 Allen Wrench</li> <li>Syringe</li> <li>Syringe Needle</li> </ul> <h3>Related Procedures</h3><span class="btn-link">[[Removing the Pump Head->Removing the Pump Head - LC-2030 (313)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Cleaning and Replacing the Plunger and Diaphragm</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/i2XzZrGKrYs?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Warnings</h3> <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>Do NOT allow foreign particles or crystals into the head holder. Foreign particles from the mobile phase solution or crystals from the buffer solution getting into the head holder may cause scratches on the surface of the plunger.</strong> <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>Gently insert the box driver into the plunger. The plunger may become damaged if the box driver is forcefully inserted.</strong> <h3>Procedures</h3><ol> <li>Use a wiping paper soaked with 2-propanol to remove any portion of the seal or dirt attached to the plunger (A).</li> <li>Check that there are no visible scratches on the plunger (A) or the diaphragm (B).</li> <li> If no scratches are found through visual inspection, proceed to [[Replacing the Plunger and Diaphragm/Replace the Plunger Seal.->Replacing the Plunger and Diaphragm (321)]]</li> <li>If scratches are found through visual inspection, replace with a new plunger, following these steps.</li> <li>Insert the box driver (A) into the plunger holder (B), then rotate counter-clockwise to remove it.</li> <li>Attach a new diaphragm (B) to the new plunger holder (A) using the diaphragm jig (C).</li> <li>After applying the grease (black) provided with the plunger holder ASSY to the rear end surface of the plunger (a), insert the plunger holder (A) to which the diaphragm was attached into the pump body.</li> <li>Tighten the plunger holder (B) using the box driver (A).</li> <li>Next, use a piece of wiping paper soaked in 2-propanol to wipe and clean the plunger surface.</li> <li>Please proceed to [[Replacing the Plunger and Diaphragm/Replace the Plunger Seal.->Replacing the Plunger and Diaphragm (321)]]</li> </ol> <h3>Related Notes</h3><ul> <li>If part of the seal remains attached, airtightness cannot be maintained.</li> <li>Confirm the direction of the diaphragm when attaching.</li> <li>Be careful not to get any grease on the diaphragm or the body.</li> <li>Attach the diaphragm so that it will not become detached from the plunger holder. If the diaphragm becomes detached, wipe away any grease and reattach itusing the diaphragm jig as described.</li> </ul> <h3>Necessary Parts</h3>Plunger Holder ASSY Maintenance Part 228-35281-97 Qty: 1 Remarks: 95 Gasket (Set of 2) Be sure to replace when disassembling and cleaning the flow cell. *1 <h3>Necessary Tools</h3>Box Driver 228-18886-01 Diaphragm Jig 228-54852 Seal Tool S228-25142-01 <h3>Related Procedures</h3><span class="btn-link">[[Attaching the Pump Head->Attaching the Pump Head - LC-2030 (314)]] </span> <span class="btn-link">[[Inspecting the Plunger and Diaphragm->Inspecting the Plunger and Diaphragm (319)]]</span> <span class="btn-link">[[Removing the Pump Head->Removing the Pump Head - LC-2030 (313)]]</span> <span class="btn-link">[[Replacing the Plunger and Diaphragm->Replacing the Plunger and Diaphragm (321)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Inspecting the Plunger and Diaphragm</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/uqd4UhiMHIA?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Warnings</h3> <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>Gently pull the head holder horizontally along the plunger. If the head holder is not removed carefully, the plunger may become damaged.</strong> <h3>Procedures</h3><ol> <li>Press [Plunger & Diaphragm] on the [Replacement PartsSelection] screen. The [Plunger and diaphragm replacement] screen is displayed.</li> <li>Press [Right], and then press [Next]. The pump operates and the plunger on the right side moves toward the back of the instrument. The next screen is displayed.</li> <li>[[Removing the Pump Head->Removing the Pump Head - LC-2030 (313)]]</li> <li>Remove the head holder. Loosen the head holder top and bottom fittings (A, B), and remove the rinse tube.</li> <li>Gradually loosen the 2 hexagonal socket screws (B) of the head holder (A) using an Allen wrench (M4)</li> <li>By alternating between the left and right screw, and remove the head holder.</li> <li>If head holder (A) is difficult to remove, it can be loosened by pushing the pump body (C) using the 2 hexagonal socket screws (B).</li> <li>Insert into the head holder M4 bolt hole (a) the 2 hexagonal socket screws (B) that were removed,</li> <li>Tighten the left and right sides gradually using an Allen wrench (M4) (D).</li> <li>Press [Next]. The next screen is displayed. Proceed to [[Cleaning and Replacing the Plunger and Diaphragm->Cleaning and Replacing the Plunger and Diaphragm (320)]]</li> </ol> <h3>Related Notes</h3>Remove the pump head after the [Pump] display turns off. <h3>Necessary Tools</h3><ul> <li>10mm Wrench</li> <li>8mm Wrench</li> <li>M4 Allen Wrench</li> <li>M5 Allen Wrench</li> </ul> <h3>Related Procedures</h3> <span class="btn-link">[[Attaching the Pump Head->Attaching the Pump Head - LC-2030 (314)]]</span> <span class="btn-link">[[Cleaning and Replacing the Plunger and Diaphragm->Cleaning and Replacing the Plunger and Diaphragm (320)]]</span> <span class="btn-link">[[Displaying the Replaced Part Items->Displaying the Replaced Part Items (351)]]</span> <span class="btn-link">[[Removing the Pump Head->Removing the Pump Head - LC-2030 (313)]]</span> <span class="btn-link">[[Replacing the Plunger and Diaphragm->Replacing the Plunger and Diaphragm (321)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Removing the Pump Head - LC-2030</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/Zj0sQiHk3y4?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Warnings</h3> <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>Gently insert the pump head horizontally along the plunger. The plunger may be damaged if the pump head is forcefully inserted.</strong> <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>Gently pull the pump head horizontally along the plunger. The plunger may be damaged if the pump head is forcefully removed.</strong> <h3>Procedures</h3><ol> <li>Open the pump door.</li> <li>While securing the outlet check valve (A) with a 10 mm wrench (B)</li> <li>Loosen the male nut (C) of the SUS pipe R outlet check valve with an 8 mm wrench (D).</li> <li>Loosen the male nut (A) of the SUS pipe R (B) pressure sensor with an 8 mm wrench, and remove SUS pipe R (B).</li> <li>Loosen and remove the male nut PEEK (A) of the inlet tube by hand.</li> <li>Remove the rinsing kit cap (B) from the rinse solution bottle (A).</li> <li>Attach the syringe needle (C) to the end of the outlet FEP tubing (E) (upward U-shape)</li> <li>Use the syringe (D) to suction out the rinse fluid from the rinse flow line.</li> <li>Place the removed cap (B) and rinse solution bottle on the reservoir tray on the top of the instrument.</li> <li>Gradually loosen the 2 hexagonal socket screws (A) using an Allen wrench (M5)</li> <li>By alternating between the left and right screw, and remove the pump head (B).</li> </ol> <h3>Necessary Tools</h3><ul> <li>10mm Wrench</li> <li>8mm Wrench</li> <li>M5 Allen Wrench</li> <li>Syringe</li> <li>Syringe Needle</li> </ul> <h3>Related Procedures</h3> <span class="btn-link">[[Attaching the Pump Head->Attaching the Pump Head - LC-2030 (314)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Cleaning of the Automatic Rinsing Kit</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/oxKH-kioypY?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Procedures</h3><ol> <li>Remove the rinse cap of the automatic rinsing kit from the rinse solution bottle.</li> <li>Pour distilled water into the rinse solution bottle and clean the inside of the rinse solution bottle using a brush.</li> <li>Pour distilled water into the rinse solution bottle and rinse the inside of the rinse solution bottle for about 5 minutes using an ultrasonic bath.</li> <li>Pour new 10% 2-propanol water into the rinse solution bottle and return the cap and rinse solution bottle back to their original positions.</li> <li>Replace the rinse tube if the inside is dirty.</li> </ol> <h3>Related Notes</h3>If the 10% 2-propanol water in the rinse solution bottle is dirty, replace with new solution. Even if it does not appear dirty, replace with new solution once a week. <h3>Necessary Parts</h3>Rinse Flow Line ASSY Replacement Part 228-56201-41 Qty: 1 <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Inspecting the Check Valve</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/vmYsIGP2OM8?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Procedures</h3><ol> <li>Check the pumping stability.</li> <li>Display the [Performance Check] screen</li> <li>Press the [Pump] tab and then press [Pressure pulsation]. The [Pressure pulsation] screen is displayed.</li> <li>Open the column oven door.</li> <li>Install the resistor tube (A) as shown and set water as the mobile phase.</li> <li>If the resistor tube (A) is long and difficult to directly connect to the detector, attach the column clamp (B) as shown to connect to the detector. (C: Male nut, D: Coupling, a: Detector inlet side)</li> <li>Close the column oven door.</li> <li>Press [Next].</li> <li>Select a mobile phase port to be used, and then press [Next].</li> <li>Press [Start]. Purging starts and the screen showing the purge progress is displayed.</li> <li>When purging has finished, or after pressing [Next], pumping starts.</li> <li>Check the chromatogram, and after pumping has stabilized, press [Next].</li> <li>The screen for measuring the pressure fluctuation is displayed. When measurement has finished, the message "Measurement was completed" is displayed.</li> <li>Press [Next]. The results of system checks are displayed.</li> <li>Check the results of system checks and press [Finish].</li> <li>If the result of the system check is "Pass," it is considered normal.</li> <li>If the result of the system check is "Fail", perform Rinsing the Check Valve or Performing an Ultrasonic Cleaning of the Check Valve.</li> <li>Check the pumping stability again. If the pumping (pressure) is still unstable, proceed to [[Replacing the Check Valve->Replacing the Check Valve (1196)]].</li> </ol> <h3>Related Procedures</h3> <span class="btn-link">[[Replacing the Check Valve->Replacing the Check Valve (1196)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Replacing the Check Valve</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/aD2T7PSEv8w?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Warnings</h3> <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>Never disassemble the check valve. Performance cannot be guaranteed if disassembled.</strong> <h3>Procedures</h3>If you have not already done so, please see [[Inspecting the Check Valve->Inspecting the Check Valve (326)]] before watching this movie.<ol> <li>Press [Check Valve(R)] on the [Replacement Parts Selection] screen. The [Check valve replacement] screen is displayed.</li> <li>Select [Right], and then press [Next]. The next screen is displayed.</li> <li>Remove the SUS tubing and the inlet tube.</li> <li>Press [Next]. The next screen is displayed.</li> <li>Replace the check valve. Use a 10 mm wrench to remove the inlet check valve (A) and the outlet check valve (B). Never disassemble the check valve.</li> <li>Attach a new inlet check valve (A) and a new outlet check valve (B) to the pump head, and after tightening by hand, use a 10 mm wrench to tighten them 90º.</li> <li>Press [Next]. The next screen is displayed.</li> <li>Attach the SUS tubing and the inlet tube.</li> <li>Press [Next]. The next screen is displayed.</li> <li>Select the replaced part and press [Next]. The next screen is displayed.</li> <li>Select the mobile phase that will be used for purging, and then press [Next]. The next screen is displayed.</li> <li>When replacing the check valve on the left side (when viewed from the front), press [Stop] and start from Step 1. After replacing that check valve, perform a purge.</li> <li>Press [Start]. Purging starts. After purging finishes, the next screen is displayed automatically.</li> <li>Press [Finish]. The replacement history is recorded in the maintenance log.</li> </ol> <h3>Related Notes</h3>When proceeding to replace the check valve on the left side (when viewed from the front), press [Stop] and return to Procedure 1, and then, after replacing the check valve in the same procedure, perform a purge. <h3>Necessary Parts</h3>Inlet Check Valve ASSY Maintenance Part 228-48249-96 Qty: 1 Outlet Check Valve ASSY Maintenance Part 228-45705-91 Qty: 1 <h3>Necessary Tools</h3><ul> <li>10mm Wrench</li> <li>8mm Wrench</li> </ul> <h3>Related Procedures</h3> <span class="btn-link">[[Attaching the Pump Head->Attaching the Pump Head - LC-2030 (314)]]</span> <span class="btn-link">[[Displaying the Replaced Part Items->Displaying the Replaced Part Items (351)]]</span> <span class="btn-link">[[Inspecting the Check Valve->Inspecting the Check Valve (326)]]</span> <span class="btn-link">[[Removing the Pump Head->Removing the Pump Head - LC-2030 (313)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Displaying the Replaced Part Items</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/SZsqFrwjkog?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Procedures</h3><ol> <li>Press (Menu). The [Menu] screen opens.</li> <li>Press [Maintenance]. The [Maintenance] screen is displayed. The tab for the replaced part is displayed first.</li> <li>Press the button to select each unit. The [Replacement Parts Selection] screen is displayed. The tab for the selected unit is displayed.</li> <li>Press the button with the corresponding name to display the replacement screen for a main part.</li> <li>Press an item of the part for replacement.</li> <li>Carry out the operation by following the explanation in the screen. Operations vary depending on each item.</li> </ol> <h3>Related Notes</h3><ul> <li>Press (Replacement part) to display the tab for the replaced part when other tabs are displayed in the [Maintenance] screen.</li> <li>When a service duration for a part in the unit exceeds the replacement guidelines settings, (Warning!) is displayed.</li> <li>If a W lamp is connected, press [D2 Lamp / W Lamp] once to alternatively switch the [D2 Lamp] and [W Lamp] displays. Pressing the button displayed as "Usage frequency / Replacement guidelines" displays the replacement screen for the corresponding part.</li> </ul> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Inspecting/Cleaning/Replacing the Suction Filter - Pump</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/H7ToGO-Zbo0?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Warnings</h3> <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>After using the buffer solution as the mobile phase, do NOT replace with an organic solvent such as 2-propanol without first replacing with water. Crystals, etc., that cannot be removed may appear.</strong> <h3>Procedures</h3><ol> <li>Pull the suction filter (A) from the suction tubing (B).</li> <li>Place the suction filter into a clean container with 2-propanol and clean it for 5 minutes using an ultrasonic bath.</li> <li>Insert the suction tubing (A) into the suction filter (B).</li> <li>Replace the solvent in reservoir bottle A with water, and place the suction tubing back into the bottle.</li> <li>Press [Purge] on the main analysis screen. The [Auto Purge] screen is displayed.</li> <li>Select [1st] only, and set [A] to [10 min].</li> <li>Press [Start]. Purging starts.</li> <li>Check for air bubbles inside the suction tubing. Replace the suction filter if air bubbles are present.</li> <li>[[Record the replacement history in the maintenance log.->Recording Replacement History in the Replacement Log (352)]]</li> </ol> <h3>Related Notes</h3>When using a buffer solution, replace it with distilled water or purified water, and after pumping at least 20 mL into the instrument, replace it with new 2-propanol. If the buffer solution is directly replaced with 2-propanol, crystals will be precipitated, which can cause damage to the plunger seal or cause problems with the check valve. <h3>Necessary Parts</h3>Suction Filter Consumable Part 228-45707-91 Qty: 1 <h3>Related Procedures</h3> <span class="btn-link">[[Recording Replacement History in the Replacement Log->Recording Replacement History in the Replacement Log (352)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Recording Replacement History in the Replacement Log</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/SUBfeIyoUDE?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Procedures</h3><ol> <li>Press (Menu). The [Menu] screen opens.</li> <li>Press [Maintenance]. The [Maintenance] screen is displayed. The tab for the replaced part is displayed first.</li> <li>Press [Others]. The [Replacement Parts Selection] screen is displayed. The [Others] tab is displayed.</li> <li>Press [Others]. The [Other Part Replacement] screen is displayed.</li> <li>Input [P/N] (Part No.), then press [OK]. The confirmation screen is displayed.</li> <li>Check the part No., then press [OK]. The input part No. is recorded in the maintenance log.</li> </ol> <h3>Related Notes</h3><ul> <li>Press (Replacement part) to display the tab for the replaced part when other tabs are displayed in the [Maintenance] screen.</li> <li>When replacement of a part inside the unit is necessary, (Warning button) is displayed.</li> </ul> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Performing an Ultrasonic Cleaning of the Check Valve</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/ICvvTMPU_IM?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Warnings</h3> <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>Never disassemble the check valve. Performance cannot be guaranteed if disassembled.</strong> <h3>Procedures</h3><ol> <li>Open the pump door. Remove the SUS tubing and the inlet tube.</li> <li>While securing the outlet check valve (A) with a 10 mm wrench (B), loosen the male nut (C) of the SUS pipe R outlet check valve with an 8 mm wrench (D).</li> <li>Loosen the male nut (A) of the SUS pipe R (B) pressure sensor with an 8 mm wrench, and remove SUS pipe R (B).</li> <li>Loosen and remove the male nut PEEK (A) of the inlet tube by hand.</li> <li>Remove the check valve. Use a 10 mm wrench to remove the inlet check valve (A) and the outlet check valve (B).</li> <li>Immerse the inlet check valve and the outlet check valve into 2-propanol and rinse for 5 minutes using an ultrasonic bath.</li> <li>Rotate the inlet check valve (A) and the outlet check valve (B) clockwise to attach them to the pump head</li> <li>After tightening by hand, use a 10 mm wrench to tighten them 90°.</li> <li>Tighten the male nut PEEK (A) of the inlet tube to the inlet check valve (B) by hand.</li> <li>Tighten the male nut (A) of the SUS pipe R (B) pressure sensor with an 8 mm wrench, and attach SUS pipe R (B).</li> <li>While securing the outlet check valve (A) with a 10 mm wrench (B), tighten the male nut (C) of the SUS pipe R outlet check valve with an 8 mm wrench (D).</li> </ol> <h3>Necessary Tools</h3><ul> <li>10mm Wrench</li> <li>8mm Wrench</li> </ul> <h3>Related Procedures</h3> <span class="btn-link">[[Attaching the Pump Head->Attaching the Pump Head - LC-2030 (314)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Pump Pressure Zero Adjustment</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/TiqOnuWVb04?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Procedures</h3><ol> <li>Press [Pump Pressure Zero Adjustment] on the [Auxiliary Operation] screen. The [Pump Pressure Zero Adjustment] screen is displayed.</li> <li>Press [Execute]. Adjust the pump pressure to "0".</li> <li>Check that the pump pressure monitor value at the top of the screen becomes "0".</li> <li>Press [Close].</li> </ol> <h3>Related Procedures</h3> <span class="btn-link">[[Displaying Auxiliary Function Items->Displaying Auxiliary Function Items (357)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Displaying Auxiliary Function Items</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/VA9EOBvD5KI?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Procedures</h3><ol> <li>Press (Menu). The [Menu] screen opens.</li> <li>Press [Maintenance]. The [Maintenance] screen is displayed.</li> <li>Press (Validation). The [Validation] tab is displayed.</li> <li>Press [Auxiliary Operation]. The [Auxiliary Operation] screen is displayed.</li> <li>Press the target item.</li> </ol> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Removing the Pump Head - LC-2040</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/fcHjF0ej5gA?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Procedures</h3><ol> <li>Open the pump door.</li> <li>While securing the outlet check valve (A) with a 10 mm wrench (B)</li> <li>Loosen the male nut (C) of the SUS pipe R outlet check valve with an 8 mm wrench (D).</li> <li>Loosen the male nut (A) of the SUS pipe R (B) pressure sensor with an 8 mm wrench, and remove SUS pipe R (B).</li> <li>Loosen and remove the male nut PEEK (A) of the inlet tube by hand.</li> <li>Remove the rinsing kit cap (B) from the rinse solution bottle (A).</li> <li>Attach the syringe needle (C) to the end of the outlet FEP tubing (E) (upward U-shape),</li> <li>Use the syringe (D) to suction out the rinse fluid from the rinse flow line.</li> <li>Place the removed cap (B) and rinse solution bottle on the reservoir tray on the top of the instrument.</li> <li>Gradually loosen the 2 hexagonal socket screws (A) using an Allen wrench (M5)</li> <li>By alternating between the left and right screw, and remove the pump head (B).</li> </ol> <h3>Necessary Tools</h3><ul> <li>10mm Wrench</li> <li>8mm Wrench</li> <li>M5 Allen Wrench</li> <li>Syringe</li> <li>Syringe Needle</li> </ul> <h3>Related Procedures</h3> <span class="btn-link">[[Removing the Pump Head->Removing the Pump Head - LC-2030 (313)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Replacing the Drain Valve ASSY</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/Sn49WT624Io?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Procedures</h3><ol> <li>Open the pump door.</li> <li>Rotate the drain valve ASSY (A) counter-clockwise at least 3 complete rotations and pull it off straight.</li> <li>Remove the cap from the knob, and with a Phillips screwdriver remove the screw attaching the knob to the valve.</li> <li>Wet the seal portion (a) of the new drain valve ASSY (A) with 2-propanol and insert it into the hole.</li> <li>Tighten the drain valve ASSY (A) clockwise.</li> <li>After tightening the drain valve ASSY (A), attach the knob (B) using the screw (C) so that it is vertical,</li> <li>Then attach the cap (D).</li> <li>Close the pump door</li> <li>[[Record the replacement history in the maintenance log.->Recording Replacement History in the Replacement Log (352)]]</li> </ol> <h3>Related Notes</h3><ul> <li>Check that there are no scratches or dirt in the hole after removal.</li> <li>If the drain valve ASSY is forcefully inserted or is not inserted straight, the seal portion may become deformed, resulting in leakage.</li> </ul> <h3>Necessary Parts</h3>Drain Valve ASSY (for LC-2030) Replacement Part 228-45574-95 Qty: 1 Drain Valve ASSY (for LC-2040) Replacement Part S228-51229-93 Qty: 1 <h3>Necessary Tools</h3>#2 Phillips Screwdriver <h3>Related Procedures</h3> <span class="btn-link">[[Recording Replacement History in the Replacement Log->Recording Replacement History in the Replacement Log (352)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Replacing the Line Filter</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/9qN0UChCxI0?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Procedures</h3><ol> <li>Open the pump door.</li> <li>Secure the line filter (A) using a 10 mm wrench (B) and loosen the male nut (C) of the SUS tubing using an 8 mm wrench (D) to remove it.</li> <li>Loosen the line filter (A) with a 10 mm wrench to remove it.</li> <li>Use a cotton swab to wipe away any dirt from the line filter port (a).</li> <li>Attach the new line filter (A) to the line filter port (a), and tighten it 45° using a 10 mm wrench.</li> <li>Attach the male nut (C) of the SUS tubing to the line filter (A) by hand.</li> <li>Secure the line filter (A) using a 10 mm wrench (B)</li> <li>Tighten the male nut (C) of the SUS tubing using an 8 mm wrench (D).</li> <li>Close the pump door.</li> <li>[[Record the replacement history in the maintenance log.->Recording Replacement History in the Replacement Log (352)]]</li> </ol> <h3>Necessary Parts</h3>Line Filter Consumable Part 228-35871-96 Qty: 1 <h3>Necessary Tools</h3><ul> <li>10mm Wrench</li> <li>8mm Wrench</li> </ul> <h3>Related Procedures</h3> <span class="btn-link">[[Recording Replacement History in the Replacement Log->Recording Replacement History in the Replacement Log (352)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Replacing the Plunger Seal - Part 1 - LC-2040</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/rz4ffK0nono?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Procedures</h3><ol> <li>Press [Plunger Seal(R)] on the [Replacement Parts Selection] screen. The [Plunger seal replacement] screen is displayed.</li> <li>Select [Right], and then press [Next]. The pump operates and the plunger on the right side moves toward the back of the instrument. The next screen is displayed.</li> <li>[[Remove the pump head.->Removing the Pump Head - LC-2040 (1220)]]</li> <li>Press [Next]. The next screen is displayed.</li> <li>Vertically insert the end of the seal tool (A) with a stepped side (a) into the plunger seal (B).</li> <li>Pull out the seal tool (A) vertically to remove the plunger seal (B) from the pump head (C).</li> <li>Place the pump head into a clean beaker containing 2-propanol, and clean it for 10 minutes using an ultrasonic bath.</li> <li>Use a piece of wiping paper soaked in 2-propanol to wipe the plunger attachment portion inside the pump head (a).</li> <li>Immerse the plunger seal in a clean container with 2-propanol.</li> <li>Insert the end of the seal tool (A) with no unevenness (a) into the plunger seal (B)</li> <li>Vertically insert it into the pump head where the plunger seal attaches (b).</li> <li>Pull out the seal tool (A) vertically.</li> <li>Press [Next]. The next screen is displayed.</li> <li>Please see [[Replacing the Plunger Seal - Part 2->Replacing the Plunger Seal - Part 2 - LC-2040 (1142)]] to conclude this procedure.</li> </ol> <h3>Related Notes</h3><ul> <li>Remove the pump head after the [Pump] display turns off.</li> <li>Airtightness cannot be maintained if part of the seal, etc., is stuck to the pump head where the plunger seal attaches.</li> <li>Replace the pump head if there are scratches on the pump head where the plunger seal attaches.</li> <li>Be careful not to scratch the inner surface of the plunger seal with the seal tool.</li> </ul> <h3>Necessary Parts</h3>Plunger Seal Consumable Part 228-35146 Qty: 1 <h3>Necessary Tools</h3><ul> <li>10mm Wrench</li> <li>8mm Wrench</li> <li>M5 Allen Wrench</li> <li>Seal Tool S228-25142-01</li> </ul> <h3>Related Procedures</h3> <span class="btn-link">[[Removing the Pump Head - LC-2040->Removing the Pump Head - LC-2040 (1220)]]</span> <span class="btn-link">[[Replacing the Plunger Seal - Part 2->Replacing the Plunger Seal - Part 2 - LC-2040 (1142)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Replacing the Plunger Seal - Part 2 - LC-2040</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/-f4cZ6AXtj8?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Procedures</h3>If you have not already done so, please see [[Replacing the Plunger Seal - Part 1->Replacing the Plunger Seal - Part 1 - LC-2040 (1217)]] before viewing this video.<ol> <li> [[Attach the Pump Head.->Attaching the Pump Head - LC-2040 (1219)]]</li> <li>Press [OK] .The usage value is reset. The replacement history is recorded in the maintenance log.</li> <li>Check that the usage value is reset and then press [Next]. The next screen is displayed.</li> <li>Select the mobile phase that will be used for purging, and then manually purge the pump.</li> <li>Press [Next]. The next screen is displayed.</li> <li>Press [Start]. Purging starts. After purging finishes, the next screen is displayed automatically.</li> <li>After replacing the plunger seal, perform a check.</li> <li>Is the pumping (pressure) stable?</li> <li>Is fluid leaking from the gap between the pump head and the head holder?</li> <li>Press [Exit].</li> </ol> <h3>Related Notes</h3><ul> <li>If the above occurs even after replacing the plunger seal, it is possible that there are scratches on the surface of the plunger. If there are scratches on the plunger, replace it with a new plunger.</li> <li>When proceeding to replace the plunger seal on the left side (when viewed from the front), press [Stop] and return to Procedure 1, and then, after replacing the plunger seal in the same procedure, perform a purge.</li> </ul> <h3>Related Procedures</h3> <span class="btn-link">[[Attaching the Pump Head - LC-2040->Attaching the Pump Head - LC-2040 (1219)]]</span> <span class="btn-link">[[Cleaning and Replacing the Plunger and Diaphragm->Cleaning and Replacing the Plunger and Diaphragm (320)]]</span> <span class="btn-link">[[Replacing the Plunger Seal - Part 1->Replacing the Plunger Seal - Part 1 - LC-2040 (1217)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Rinsing the Check Valve</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/E4eq-cmGYfg?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Warnings</h3> <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>After using the buffer solution as the mobile phase, do NOT replace with an organic solvent such as 2-propanol without first replacing with water. Crystals, etc., that cannot be removed may appear.</strong> <h3>Procedures</h3><ol> <li>Replace the solvent in reservoir bottle A with 2-propanol.</li> <li>Open the column oven door.</li> <li>Loosen the male nut (A) of the column inlet with an 8 mm wrench</li> <li>Loosen the male nut (C) of the column outlet by hand, and remove the column (A) from the flow line.</li> <li>Attach the male nut (A) of the column inlet that was removed to the resistor tube (D) and tighten it with an 8 mm wrench.</li> <li>Attach the other male nut (C) of the column outlet to the resistor tube (D), and tighten it by hand.</li> <li>From the Main Analysis screen, set so that pumping is only performed for port A.</li> <li>If the pumping mode is [ISO], set the mobile phase port to [A].</li> <li>If the pumping mode is [GRAD.], set the concentration for [B], [C], and [D] to "0".</li> <li>Set the flow rate to "2 mL/min".</li> <li>Press [Pump]. 2-propanol is pumped at a rate of 2 mL/min. Perform pumping for at least 1 hour.</li> <li>Loosen the male nut (A) of the resistor tube inlet with an 8 mm wrench</li> <li>Loosen the male nut (C) of the resistor tube outlet by hand, and remove the resistor tube (B) from the flow line</li> <li>Attach the male nut (A) of the resistor tube inlet that was removed to the column (D) with an 8 mm wrench</li> <li>Tighten it with the 8 mm wrench.</li> <li>Attach the other male nut (C) of the resistor tube outlet to the resistor tube (D), and tighten it by hand.</li> <li>Close the column oven door.</li> <li>Replace the solvent in reservoir bottle A with the original mobile phase.</li> <li>Insert the suction filter into reservoir bottle A.</li> </ol> <h3>Related Notes</h3>When using a buffer solution in the reservoir bottle, replace with distilled water or purified water, and after pumping at least 20 mL into the instrument, replace with new 2-propanol. If the buffer solution is directly replaced with 2-propanol, crystals will be precipitated, which can cause damage to the plunger seal or cause problems ith the check valve. <h3>Necessary Parts</h3>Resistor Tube Replacement Part 228-57908-41 Qty: 1 <h3>Necessary Tools</h3>8mm Wrench <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Replacing the Plunger Seal - Part 1 - LC-2030</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/Njum69hK96c?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Procedures</h3><ol> <li>Press [Plunger Seal(R)] on the [Replacement Parts Selection] screen. The [Plunger seal replacement] screen is displayed.</li> <li>Select [Right], and then press [Next]. The pump operates and the plunger on the right side moves toward the back of the instrument. The next screen is displayed.</li> <li>[[Removing the Pump Head - LC-2030->Removing the Pump Head - LC-2030 (313)]]</li> <li>Press [Next]. The next screen is displayed.</li> <li>Vertically insert the end of the seal tool (A) with a stepped side (a) into the plunger seal (B).</li> <li>Pull out the seal tool (A) vertically to remove the plunger seal (B) from the pump head (C).</li> <li>Place the pump head into a clean beaker containing 2-propanol, and clean it for 10 minutes using an ultrasonic bath.</li> <li>Use a piece of wiping paper soaked in 2-propanol to wipe the plunger attachment portion inside the pump head (a).</li> <li>Immerse the plunger seal in a clean container with 2-propanol.</li> <li>Insert the end of the seal tool (A) with no unevenness (a) into the plunger seal (B), and vertically insert it into the pump head where the plunger seal attaches (b).</li> <li>Pull out the seal tool (A) vertically.</li> <li>Press [Next]. The next screen is displayed.</li> <li>[[Attach the pump head->Attaching the Pump Head - LC-2030 (314)]]</li> <li>Please see [[Replacing the Plunger Seal - Part 2->Replacing the Plunger Seal - Part 2 - LC-2030 (1218)]] to conclude this procedure.</li> </ol> <h3>Related Notes</h3><ul> <li>Remove the pump head after the [Pump] display turns off.</li> <li>Airtightness cannot be maintained if part of the seal, etc., is stuck to the pump head where the plunger seal attaches.</li> <li>Replace the pump head if there are scratches on the pump head where the plunger seal attaches.</li> <li>Be careful not to scratch the inner surface of the plunger seal with the seal tool.</li> </ul> <h3>Necessary Parts</h3>Plunger Seal Consumable Part 228-35146 Qty: 1 <h3>Necessary Tools</h3><ul> <li>10mm Wrench</li> <li>8mm Wrench</li> <li>M5 Allen Wrench</li> <li>Seal Tool S228-25142-01</li> </ul> <h3>Related Procedures</h3> <span class="btn-link">[[Replacing the Plunger Seal - Part 2->Replacing the Plunger Seal - Part 2 - LC-2030 (1218)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Replacing the Plunger Seal - Part 2 - LC-2030</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/-4dDtVxz0SA?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Procedures</h3>If you have not already done so, please see [[Replacing the Plunger Seal - Part 1->Replacing the Plunger Seal - Part 1 - LC-2030 (322)]] before viewing this video.<ol> <li>Press [Next]. The next screen is displayed and the usage value is reset. The replacement history is recorded in the maintenance log.</li> <li>Check that the usage value is reset and then press [Next]. The next screen is displayed.</li> <li>Select the mobile phase that will be used for purging, and then manually purge the pump.</li> <li>Insert the syringe with needle (B) into the mobile phase drain tube (A) marked with "P" (a).</li> <li>Open the pump door, rotate the drain valve knob 90 degrees counterclockwise, and open the drain valve.</li> <li>Aspirate the mobile phase with the syringe.</li> <li>Close the drain valve by rotating the drain valve knob clockwise until the rotation stops.</li> <li>Close the pump door.</li> <li>Remove the syringe.</li> <li>Press [Next]. The next screen is displayed.</li> <li>Press [Start]. Purging starts. After purging finishes, the next screen is displayed automatically.</li> <li>After replacing the plunger seal, perform a check.</li> <li>Is the pumping (pressure) stable?</li> <li>Is fluid leaking from the gap between the pump head and the head holder?</li> <li>Press [Exit].</li> </ol> <h3>Related Notes</h3><ul> <li>If the above occurs even after replacing the plunger seal, it is possible that there are scratches on the surface of the plunger. If there are scratches on the plunger, replace it with a new plunger.</li> <li>When proceeding to replace the plunger seal on the left side (when viewed from the front), press [Stop] and return to Procedure 1, and then, after replacing the plunger seal in the same procedure, perform a purge.</li> </ul> <h3>Related Procedures</h3> <span class="btn-link">[[Cleaning and Replacing the Plunger and Diaphragm->Cleaning and Replacing the Plunger and Diaphragm (320)]]</span> <span class="btn-link">[[Replacing the Plunger Seal - Part 1->Replacing the Plunger Seal - Part 1 - LC-2030 (322)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>