i-Series Autosampler Periodic Maintenance

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<div class="container"><h1 class="title">Autosampler Periodic Maintenance</h1> <h3>Select a periodic maintenance procedure below to learn more.</h3> <span class="btn-link">[[Attaching the Front Panel->Attaching the Front Panel (292)]]</span> <span class="btn-link">[[Cleaning/Inspecting/Replacing the Suction Filter->Cleaning/Inspecting/Replacing the Suction Filter (310)]]</span> <span class="btn-link">[[High-Pressure Valve Operation Check->High-Pressure Valve Operation Check (360)]]</span> <span class="btn-link">[[Injection Port Operation Check->Injection Port Operation Check (363)]]</span> <span class="btn-link">[[Inspecting and Replacing the Low Pressure Valve Rotor and Low Pressure Valve Stator - LC-2040->Inspecting and Replacing the Low Pressure Valve Rotor and Low Pressure Valve Stator - LC-2040 (1212)]]</span> <span class="btn-link">[[Inspecting and Replacing the Low Pressure Valve Rotor and Low Pressure Valve Stator - LC-2030->Inspecting/Replacing the Low-Pressure Valve Rotor and Low-Pressure Valve Stator - LC-2030 (305)]]</span> <span class="btn-link">[[Inspecting the High Pressure Valve Rotor and the High Pressure Valve Stator - LC-2040->Inspecting the High Pressure Valve Rotor and the High Pressure Valve Stator - LC-2040 (1206)]]</span> <span class="btn-link">[[Inspecting the High Pressure Valve Rotor and the High Pressure Valve Stator - LC-2030->Inspecting the High-Pressure Valve Rotor and the High-Pressure Valve Stator - LC-2030 (296)]]</span> <span class="btn-link">[[Inspecting/Cleaning/Replacing the Suction Filter - Autosampler->Inspecting/Cleaning/Replacing the Suction Filter - Autosampler (331)]]</span> <span class="btn-link">[[Removing the Front Panel->Removing the Front Panel (291)]]</span> <span class="btn-link">[[Replacing the Front Panel->Replacing the Front Panel(308)]]</span> <span class="btn-link">[[Replacing the High Pressure Valve Rotor and High Pressure Valve Stator - Part 1 - LC-2040->Replacing the High Pressure Valve Rotor and High Pressure Valve Stator - Part 1 - LC-2040 (1207)]]</span> <span class="btn-link">[[Replacing the High Pressure Valve Rotor and High Pressure Valve Stator - Part 2 - LC-2040->Replacing the High Pressure Valve Rotor and High Pressure Valve Stator - Part 2 - LC-2040 (1208)]]</span> <span class="btn-link">[[Replacing the High Pressure Valve Rotor and High Pressure Valve Stator - Part 3 - LC-2040->Replacing the High Pressure Valve Rotor and High Pressure Valve Stator - Part 3 - LC-2040 (1209)]]</span> <span class="btn-link">[[Replacing the High Pressure Valve Rotor and High Pressure Valve Stator - Part 1 - LC-2030->Replacing the High-Pressure Valve Rotor and High-Pressure Valve Stator - Part 1 - LC-2030 (297)]]</span> <span class="btn-link">[[Replacing the High-Pressure Valve Rotor and High-Pressure Valve Stator - Part 2 - LC-2030->Replacing the High-Pressure Valve Rotor and High-Pressure Valve Stator - Part 2 - LC-2030 (298)]]</span> <span class="btn-link">[[Replacing the Measuring Pump Plunger - LC-2040 ->Replacing the Measuring Pump Plunger - LC-2040 (1211)]]</span> <span class="btn-link">[[Replacing the Measuring Pump Plunger Seal - LC-2040 ->Replacing the Measuring Pump Plunger Seal - LC-2040(1210)]]</span> <span class="btn-link">[[Replacing the Measuring Pump Plunger Seal - LC-2030 ->Replacing the Measuring Pump Plunger Seal - LC-2030(301)]]</span> <span class="btn-link">[[Replacing the Needle - Part 1->Replacing the Needle - Part 1 (294)]]</span> <span class="btn-link">[[Replacing the Needle - Part 2->Replacing the Needle - Part 2 (295)]]</span> <span class="btn-link">[[Replacing the Needle Seal->Replacing the Needle Seal (293)]]</span> <span class="btn-link">[[Replacing the Outlet Tubing->Replacing the Outlet Tubing (312)]]</span> <span class="btn-link">[[Replacing the Rack Panel->Replacing the Rack Panel (307)]]</span> <span class="btn-link">[[Replacing the Rinsing Port Cap->Replacing the Rinsing Port Cap (306)]]</span> <span class="btn-link">[[Replacing the Sample Loop - Part 1->Replacing the Sample Loop - Part 1 (276)]]</span> <span class="btn-link">[[Replacing the Sample Loop - Part 2->Replacing the Sample Loop - Part 2 (299)]]</span> <span class="btn-link">[[Replacing the Sample Loop - Part 3->Replacing the Sample Loop - Part 3 (300)]]</span> <span class="btn-link">[[Replacing the Wiper->Replacing the Wiper (309)]]</span> <span class="btn-link">[[Rinsing the Flow Line->Rinsing the Flow Line (311)]]</span> </div>

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<div class="container"><h1 class="title">Attaching the Front Panel</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/fCSO_CMg48o?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Procedures</h3><ol> <li>Align the groove (b) on the bottom of the front panel with the protrusion on the main body frame (a) to insert the front panel.</li> <li>Insert the bundle of tubes (C) into the opening (c) on the left side of the front panel.</li> <li>Attach the 4 knurled screws (A).</li> <li>Attach the removed sample racks (A) and close the panels and doors (B).</li> </ol> <h3>Related Notes</h3>If the front panel (A) is not attached correctly, condensation may settle on the rack plate. <h3>Related Procedures</h3> <span class="btn-link">[[Removing the Front Panel->Removing the Front Panel (291)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Cleaning/Inspecting/Replacing the Suction Filter</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/S1ZShKj_-Do?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Warnings</h3> <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>After using the buffer solution as the mobile phase, do NOT replace with an organic solvent such as 2-propanol without first replacing with water. Crystals, etc., that cannot be removed may appear.</strong> <h3>Procedures</h3><ol> <li>Pull the suction filter (A) from the suction tubing (B).</li> <li>Place the suction filter into a clean container with 2-propanol, and clean it for 5 minutes using an ultrasonic bath.</li> <li>Insert the suction tubing (A) into the suction filter (B).</li> <li>Replace the solvent in reservoir bottle A with water, and place the suction tubing back into the bottle.</li> <li>Press [Purge] on the main analysis screen. The [Auto Purge] screen is displayed.</li> <li>Select [1st] only, and set [A] to [10 min].</li> <li>Press [Start]. Purging starts.</li> <li>Check for air bubbles inside the suction tubing. Replace the suction filter if air bubbles are present.</li> <li>Record the replacement history in the maintenance log.</li> </ol> <h3>Related Notes</h3>When using a buffer solution, replace it with distilled water or purified water, and after pumping at least 20 mL into the instrument, replace it with new 2-propanol. If the buffer solution is directly replaced with 2-propanol, crystals will be precipitated, which can cause damage to the plunger seal or cause problems with the check valve. <h3>Necessary Parts</h3>Suction Filter Consumable Part 228-45707-91 Qty: 1 Visit our <a href="https://store.shimadzu.com/" target="_blank">webstore</a> to order parts. <h3>Related Procedures</h3> <span class="btn-link">[[Recording Replacement History in the Replacement Log->Recording Replacement History in the Replacement Log (352)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">High-Pressure Valve Operation Check</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/DPzHaC3cdQM?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Procedures</h3><ol><li>Press [HPV Operation Check] on the [Auxiliary Operation] screen. The [HPV Operation Check] screen is displayed.</li> <li>Press [Start]. The high-pressure valve flow line switches.</li> <li>Press [Close].</li> </ol> <h3>Related Procedures</h3><span class="btn-link">[[Displaying Auxiliary Function Items->Displaying Auxiliary Function Items (357)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Injection Port Operation Check</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/1bFmGgSv5js?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Procedures</h3><ol><li>Press [Injection Port Operation Check] on the [Auxiliary Operation] screen. The [Injection Port Operation Check] screen is displayed.</li> <li>Press [Start] and check that the needle moves down to the injection port properly.</li> <li>Press [Close].</li> </ol> <h3>Related Procedures</h3><span class="btn-link">[[Displaying Auxiliary Function Items->Displaying Auxiliary Function Items (357)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Removing the Front Panel</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/Mm13DMGjO0c?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Procedures</h3><ol><li>Remove the inserted sample racks (A) and open the panels and doors ((B).</li> <li>Remove the 4 knurled screws (A), and pull the front panel (B) forward. The 2 screws on the top side are short and the 2 screws on the right side are long.</li></ol> <h3>Related Procedures</h3> <span class="btn-link">[[Attaching the Front Panel->Attaching the Front Panel (292)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Displaying Auxiliary Function Items</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/VA9EOBvD5KI?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Procedures</h3><ol><li>Press (Menu). The [Menu] screen opens.</li> <li>Press [Maintenance]. The [Maintenance] screen is displayed.</li> <li>Press (Validation). The [Validation] tab is displayed.</li> <li>Press [Auxiliary Operation]. The [Auxiliary Operation] screen is displayed.</li> <li>Press the target item.</li> </ol> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Recording Replacement History in the Replacement Log</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/SUBfeIyoUDE?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Procedures</h3><ol><li>Press (Menu). The [Menu] screen opens.</li> <li>Press [Maintenance]. The [Maintenance] screen is displayed. The tab for the replaced part is displayed first.</li> <li>Press [Others]. The [Replacement Parts Selection] screen is displayed. The [Others] tab is displayed.</li> <li>Press [Others]. The [Other Part Replacement] screen is displayed.</li> <li>Input [P/N] (Part No.), then press [OK]. The confirmation screen is displayed.</li> <li>Check the part No., then press [OK]. The input part No. is recorded in the maintenance log.</li> </ol> <h3>Related Notes</h3><ul> <li>Press (Replacement part) to display the tab for the replaced part when other tabs are displayed in the [Maintenance] screen.</li> <li>When replacement of a part inside the unit is necessary, (Warning button) is displayed.</li> </ul> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Inspecting and Replacing the Low Pressure Valve Rotor and Low Pressure Valve Stator - LC-2040 </h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/S1UiaVruYnM?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Procedures</h3><ol> <li>Press [LPV Rotor] or [LPV Stator] on the [Replacement Parts Selection] screen</li> <li>Lower the rinse solution bottle (B) so that the liquid surface (a) is lower than the low-pressure valve (A).</li> <li>Open the valve mount door.</li> <li>Loosen the 6 male nuts of the housing (A) and the 2 male nuts of the top and bottom of measurement pump head (B), using a wrench (6 mm width, 8 mm width) to remove the flow line tubing.</li> <li>Use an M4 Allen wrench to remove the 3 hexagon socket screws (A).</li> <li>Remove the low-pressure valve stator ASSY (A) and ring (B), taking care not to drop the ring (B). Place down with the ring side up.</li> <li>Hold the edge (a) of the low-pressure valve rotor (A) and remove the rotor.</li> <li>Check for scratches on the rotating and sliding surfaces (a and b) of the low-pressure valve rotor (A) and the low-pressure valve stator (B).</li> <li>If a scratch is found through visual inspection, replace with a new low-pressure valve rotor and low-pressure valve stator.</li> <li>Disassemble the low-pressure valve stator ASSY into the low-pressure valve stator (A), gasket (B), and housing (C).</li> <li>Immerse the low-pressure valve rotor (A), low-pressure valve stator (B), gasket (C), and housing (D) into a beaker filled with 2- propanol. and clean for 5 minutes in an ultrasonic bath.</li> <li>Holding the edge of the low- pressure valve rotor (A), align the notch (a) of the low-pressure valve rotor (A) with the alignment mark (b) of the shaft (B) and insert horizontally to fit them together.</li> <li>To assemble the stator ASSY, fit the gasket (B) and the low-pressure valve stator (C) to the housing (A) in that order, then fit the ring (D).</li> <li>Face the ring side upward, hold the low-pressure valve stator ASSY (A) and the ring (B), and insert into the main unit taking care not to drop the ring.</li> <li>Attach the 3 hexagon socket screws (A) to the housing (B), and use an M4 Allen wrench to gradually tighten each screw in turn.</li> <li>Reattach the flow line tubing of each low-pressure valve port and the flow line tubing of the top and bottom of measurement pump head to their original positions. Use a wrench (6 mm/8 mm) to tighten the 8 male nuts (A).</li> <li>Close the valve mount door.</li> <li>Press [Next]. The next screen is displayed.</li> <li>Select the replaced part and press [Next]. The replacement history is recorded in the maintenance log.</li> <li>Check that the usage value has reset, and then press [Exit].</li> </ol> <h3>Related Notes</h3><ul> <li>The [Low pressure valve rotor and stator replacement] screen can also be displayed by pressing [LPV Rotor] or [LPV Stator] on the replacement parts tab in the [Maintenance] screen.</li> <li>The stop plug (C) on the port 3 is not removed.</li> <li>The low-pressure valve stator is divided into the ring and the low-pressure valve stator ASSY. Remove the ring and housing slowly because the lowpressure valve stator and gasket may drop from the lowpressure valve stator ASSY.</li> <li>Any scratches can cause wear and tear in a short period of time.</li> <li>As much as possible, touch only the very edge of the lowpressure valve rotor, and after fitting, use wiping paper moistened with 2-propanol to wipe the sliding surface that contacts the low-pressure valve stator.</li> <li>Tighten the 3 hexagon socket screws alternately 90 degrees each, using the longer side of the M4 Allen wrench as a handle to firmly tighten them at the end. Do NOT tighten the hexagon socket screws unevenly.</li> </ul> <h3>Necessary Parts</h3> Low-Pressure Valve Rotor (for LC-2040) Consumable Part S228-58668-01 Qty: 1 Low-Pressure Valve Stator (for LC-2040) Consumable Part S228-58668-01 Qty: 1 Visit our <a href="https://store.shimadzu.com/" target="_blank">webstore</a> to order parts. <h3>Necessary Tools</h3><ul> <li>8mm Wrench</li> <li>6mm Wrench</li> <li>M4 Allen Wrench</li> </ul><div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Inspecting the High Pressure Valve Rotor and the High Pressure Valve Stator</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/aJ2No-R0aOQ?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Procedures</h3><ol><li>Fill the mobile phase A flow line with an organic solvent such as 2-propanol, methanol, or acetonitrile.</li> <li>Press [HPV Rotor] or [HPV Stator] on the [Replacement Parts Selection] screen. The [High pressure valve rotor and stator replacement] screen is displayed.</li> <li>Press [Power OFF]. The Confirmation screen opens. Press [OK]. The needle moves to the center of the instrument and the power is turned OFF.</li> </ol> <h3>Related Notes</h3><ul><li>After the high-pressure valve rotor and stator have been replaced, perform conditioning by pumping of the mobile phase A in order to create a smooth seal surface (switching back and forth 50 times).</li> <li>The [High pressure valve rotor and stator replacement] screen can also be displayed by pressing [HPV Rotor] or [HPV Stator] on the replacement parts tab in the [Maintenance] screen.</li></ul> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Inspecting/Cleaning/Replacing the Suction Filter - Autosampler</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/ogke8oBLQgo?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Warnings</h3> <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>After using the buffer solution as the mobile phase, do NOT replace with an organic solvent such as 2-propanol without first replacing with water. Crystals, etc., that cannot be removed may appear.</strong> <h3>Procedures</h3><ol><li>Pull the suction filter (A) from the suction tubing (B).</li> <li>Place the suction filter into a clean container with 2-propanol, and clean it for 5 minutes using an ultrasonic bath.</li> <li>Insert the suction tubing (B) into the suction filter (A).</li> <li>Perform a flow line purge.</li> <li>Open the valve mount door.</li> <li>Insert the syringe with a needle (A) into the tube coming out of low-pressure valve port No. 3 (B).</li> <li>Press [Purge] on the Main Analysis screen.</li> <li>Press [Manual Prime].</li> <li>Pull the plunger of the syringe with needle (A) and aspirate the rinse solution.</li> <li>Press [Close] to finish manual priming.</li> <li>Reset the tube and close the valve mount door.</li> <li>Check for air bubbles in the suction tubing.</li> <li>If air bubbles are found, remove the suction filter, and replace with a new suction filter.</li> <li>Record the replacement history in the maintenance log if the suction tubing is replaced.</li> </ol> <h3>Related Notes</h3> When using a buffer solution, replace it with distilled water or purified water, and after pumping at least 20 mL into the instrument, replace it with new 2-propanol. If the buffer solution is directly replaced with 2-propanol, crystals will be precipitated, which can cause damage to the plunger seal or cause problems with the check valve. <h3>Necessary Parts</h3>Suction Filter Consumable Part 228-45707-91 Qty: 1 Visit our <a href="https://store.shimadzu.com/" target="_blank">webstore</a> to order parts. <h3>Related Procedures</h3> <span class="btn-link">[[Recording Replacement History in the Replacement Log->Recording Replacement History in the Replacement Log (352)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Replacing the Front Panel</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/to9-7LF2o4Y?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Procedures</h3><ol><li>Press (Power button) to turn OFF.</li> <li>Replace the front panel by [[removing->Removing the Front Panel (291)]] the old one and then [[attaching->Attaching the Front Panel (292)]] a new one.</li> <li>Press (Power button) to turn ON.</li> <li>[[Record Replacement History in the Replacement Log->Recording Replacement History in the Replacement Log (352)]]</li></ol> <h3>Necessary Parts</h3>Front Panel Maintenance Part 228-55569-41 Qty: 1 Visit our <a href="https://store.shimadzu.com/" target="_blank">webstore</a> to order parts. <h3>Related Procedures</h3> <span class="btn-link">[[Attaching the Front Panel->Attaching the Front Panel (292)]]</span> <span class="btn-link">[[Recording Replacement History in the Replacement Log->Recording Replacement History in the Replacement Log (352)]]</span> <span class="btn-link">[[Removing the Front Panel->Removing the Front Panel (291)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Replacing the High Pressure Valve Rotor and High Pressure Valve Stator - Part 1 - LC-2040</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/F-M8QN3KMa4?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Procedures</h3><ol> <li>[[Remove the front panel->Removing the Front Panel (291)]]</li> <li>Use a 6 mm wrench to loosen all male nuts (A) of the flow line tubing and remove the tubing from the high-pressure valve.</li> <li>Use an M4 Allen wrench to loosen the 3 hexagon socket screws in the order of A, B, and C by turning each screw 60°.</li> <li>Hold the high-pressure valve stator ASSY (A), and remove.</li> <li>Hold the outside (a) of the high pressure valve rotor (A) and remove the rotor.</li> <li>Check that the rotating and sliding surfaces (a, b) of the high pressure valve stator (A) and the high-pressure valve rotor (B) have no scratches.</li> <li>Place the high-pressure valve stator (A), high-pressure valve rotor (B), into a beaker filled with 2-propanol, and clean for 5 minutes in an ultrasonic bath.</li> <li>Align the notch (a) on the high pressure valve rotor (A) with the alignment mark (b) of the shaft (B), and gently push and fit them together.</li> <li>Please proceed to [[Replacing the High Pressure Valve Rotor and High Pressure Valve Stator - Part 2->Replacing the High Pressure Valve Rotor and High Pressure Valve Stator - Part 2 - LC-2040 (1208)]]</li></ol> <h3>Related Notes</h3><ul> <li>After the high-pressure valve rotor and stator have been replaced, perform conditioning by pumping of the mobile phase A in order to create a smooth seal surface (switching back and forth 50 times).</li> <li>The [High pressure valve rotor and stator replacement] screen can also be displayed by pressing [HPV Rotor] or [HPV Stator] on the replacement parts tab in the [Maintenance] screen.</li> <li>There is no need to remove the needle seal.</li> <li>If only certain screws are loosened, the stator may become tilted, making it difficult or impossible to remove the stator or resulting in a seized screw.</li> <li>If a scratch is found through visual inspection, replace with a new high-pressure valve rotor and highpressure valve stator.</li> <li>Any scratches can cause wear and tear in a short period of time.</li> <li>As much as possible, touch only the very edge of the highpressure valve rotor. After fitting, use wiping paper moistened with 2- propanol to wipe the rotor sliding surface that contacts the sliding surface of the high-pressure valve stator.</li> </ul> <h3>Necessary Parts</h3>High-Pressure Valve Rotor (for LC-2040) Consumable Part 228-48854-00 Qty: 1 High-Pressure Valve Stator (for LC-2040) Consumable Part 228-59076-41 Qty: 1 Visit our <a href="https://store.shimadzu.com/" target="_blank">webstore</a> to order parts. <h3>Necessary Tools</h3><ul> <li>6mm Wrench</li> <li>M4 Allen Wrench</li> <li>Fixture for Rotor Replacement S228-48899-91</li> </ul> <h3>Related Procedures</h3> <span class="btn-link">[[Removing the Front Panel->Removing the Front Panel (291)]]</span> <span class="btn-link">[[Replacing the High Pressure Valve Rotor and High Pressure Valve Stator - Part 2->Replacing the High Pressure Valve Rotor and High Pressure Valve Stator - Part 2 - LC-2040 (1208)]]</span> <span class="btn-link">[[Replacing the High Pressure Valve Rotor and High Pressure Valve Stator - Part 3->Replacing the High Pressure Valve Rotor and High Pressure Valve Stator - Part 3 - LC-2040 (1209)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Replacing the High Pressure Valve Rotor and High Pressure Valve Stator - Part 2 - LC-2040</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/IcbAO4C8syQ?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Warnings</h3> <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>Make sure to use fixtures. When fixtures are not used, it is difficult to make the stator contact with the rotor closely, and may cause liquid leakage or quick consumption of the rotor/stator in a short period.</strong> <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>Use the longer side of the Allen wrench as a handle to further tighten. If the screws are inadequately tightened, the high-pressure valve stator ASSY may be installed at an angle and result in liquid leakage or cause wear and tear of the rotor and stator in a short period of time. </strong> <h3>Procedures</h3>If you have not already done so, please see [[Replacing the High Pressure Valve Rotor and High Pressure Valve Stator - Part 1->Replacing the High Pressure Valve Rotor and High Pressure Valve Stator - Part 1 - LC-2040 (1207)]] <ol><li>Use wiping paper moistened with 2-propanol to wipe the sliding surface (a) of the high-pressure valve stator.</li> <li>Fit the high-pressure valve stator (A) onto the high-pressure valve main unit. Use a finger to lightly press the center down while ensuring the high-pressure valve stater is level.</li> <li>While pressing on the high-pressure valve stator (A), insert the longer side of the Allen wrench (M4) (B) into each of the 3 hexagon socket screws (C) and lightly turn to tighten until the screw head makes contact.</li> <li>Insert the fixture for high-pressure valve rotor replacement (A) into the space (a) between the valve main body (B) and the stator (C) and push it in to the end.</li> <li>Insert the longer side of the Allen wrench (M4) (B) into each head of the 3 hexagon socket screws and tighten until the rotation of the screw stops.</li> <li> Loosen evenly each of the 3 hexagon socket screws about 45 degrees, then pull out the fixture for rotor replacement (B).</li> <li>Use an Allen wrench (M4) to tighten evenly each of the 3 hexagon socket screws 90 degrees in the order of A, B and C.</li> <li>Loosen each of the 3 hexagon screws 90 degrees from fully tightened state in the order of C, B, and A and repeat this process 2 times (loosen 180 degrees in total).</li> <li>Once again, repeat the process of tightening each screw 90 degrees in the order of A, B, and C until the bolts are completely tightened.</li> <li>Reattach the flow line tubing of each high-pressure valve port to their original positions and use a 6mm wrench to tighten the 5 male nuts. (A).</li> <li>Please proceed to [[Replacing the High Pressure Valve Rotor and High Pressure Valve Stator - Part 3->Replacing the High Pressure Valve Rotor and High Pressure Valve Stator - Part 3 - LC-2040 (1209)]]</li></ol> <h3>Related Notes</h3><ul> <li>If a scratch is found through visual inspection, replace with a new high-pressure valve rotor and highpressure valve stator.</li> <li>Any scratches can cause wear and tear in a short period of time.</li> <li>As much as possible, touch only the very edge of the highpressure valve rotor. After fitting, use wiping paper moistened with 2-propanol to wipe the rotor sliding surface that contacts the sliding surface of the high-pressure valve stator.</li></ul> <h3>Necessary Parts</h3>High-Pressure Valve Rotor (for LC-2040) Consumable Part 228-48854-00 Qty: 1 High-Pressure Valve Stator (for LC-2040) Consumable Part 228-59076-41 Qty: 1 Visit our <a href="https://store.shimadzu.com/" target="_blank">webstore</a> to order parts. <h3>Necessary Tools</h3><ul> <li>6mm Wrench</li> <li>Fixture for Rotor Replacement S228-48899-91</li> <li>M4 Allen Wrench</li> </ul> <h3>Related Procedures</h3> <span class="btn-link">[[Replacing the High Pressure Valve Rotor and High Pressure Valve Stator - Part 1->Replacing the High Pressure Valve Rotor and High Pressure Valve Stator - Part 1 - LC-2040 (1207)]]</span> <span class="btn-link">[[Replacing the High Pressure Valve Rotor and High Pressure Valve Stator - Part 3->Replacing the High Pressure Valve Rotor and High Pressure Valve Stator - Part 3 - LC-2040 (1209)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Replacing the High Pressure Valve Rotor and High Pressure Valve Stator - Part 3 - LC-2040 </h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/JgCJJi54Z2c?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Procedures</h3>If you have not already done so, please see [[Replacing the High Pressure Valve Rotor and High Pressure Valve Stator - Part 2->Replacing the High Pressure Valve Rotor and High Pressure Valve Stator - Part 2 - LC-2040 (1208)]] <ol><li>Lightly move the Z mount (A) by hand, and check that the bottom of the Z mount does not interfere with the tubing (B) connected to the high-pressure valve.</li> <li>[[Attaching the Front Panel->Attaching the Front Panel (292)]]</li> <li>Press Power button to turn ON. After the instrument starts, input the login pass ID Number and press [OK] to display the [High pressure valve rotor and stator replacement] screen.</li> <li>Select the replaced part and press [Next] and [OK]. The next screen is displayed and the usage value is reset. The replacement history is recorded in the maintenance log.</li> <li>Check that the usage value is reset and then press [Next]. The next screen is displayed.</li> <li>Press [Start]. Conditioning is performed. After conditioning is finished, the message 'Press "Exit" to complete parts replacement' is displayed.</li> <li>Press [Exit].</li></ol> <h3>Related Notes</h3><ul> <li>Move the Z mount by holding the metallic section.</li> <li>Because abrasion powder may be generated initially, be sure to remove the column before performing conditioning. To create a smooth seal surface between the high-pressure valve rotor and stator, perform conditioning by pumping of the mobile phase A at 1 mL/min (switching back and forth 50 times).</li> <li>Check for liquid leakage from gaps around the high-pressure valve.</li> </ul> <h3>Necessary Parts</h3>High-Pressure Valve Rotor (for LC-2040) Consumable Part 228-48854-00 Qty: 1 High-Pressure Valve Stator (for LC-2040) Consumable Part 228-59076-41 Qty: 1 Visit our <a href="https://store.shimadzu.com/" target="_blank">webstore</a> to order parts. <h3>Necessary Tools</h3><ul> <li>6mm Wrench</li> <li>Fixture for Rotor Replacement S228-48899-91</li> <li>M4 Allen Wrench</li> </ul> <h3>Related Procedures</h3> <span class="btn-link">[[Attaching the Front Panel->Attaching the Front Panel (292)]]</span> <span class="btn-link">[[Replacing the High Pressure Valve Rotor and High Pressure Valve Stator - Part 1->Replacing the High Pressure Valve Rotor and High Pressure Valve Stator - Part 1 - LC-2040 (1207)]]</span> <span class="btn-link">[[Replacing the High Pressure Valve Rotor and High Pressure Valve Stator - Part 2->Replacing the High Pressure Valve Rotor and High Pressure Valve Stator - Part 2 - LC-2040 (1208)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Replacing the Measuring Pump Plunger - LC-2040</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/0kR8kUxepv8?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Warnings</h3> <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>Keep the pump head level and slowly pull it out. The plunger may break if it is pulled out at an angle.</strong> <h3>Procedures</h3><ol><li>Press [Plunger] on the [Replacement Parts Selection] screen. The measuring pump plunger moves all the way back, and the [Measuring pump plunger] screen is displayed.</li> <li>Open the valve mount door.</li> <li>Use a wrench (6 mm width/8 mm width) to loosen the 4 male nuts (A).</li> <li>Remove the flow line tubing from the top and bottom of the pump head and from port 5 and the center port of the low-pressure valve.</li> <li>Use an M4 Allen wrench to remove the 2 hexagon socket screws (B) located on the pump head (A).</li> <li>Keep the pump head (A) level and slowly pull it away from the seal holder (B).</li> <li>Keep the seal holder (A) level and slowly pull it away from the plunger (B).</li> <li>Press [Plunger Operation]. The measuring pump plunger moves toward the front of the instrument.</li> <li>Push the plunger tool (A) onto the plunger (B), and turn the plunger counterclockwise to remove the plunger ASSY.</li> <li>If a scratch is found through visual inspection, replace with a new plunger.</li> <li>If the plunger is dirty, use wiping paper moistened with 2-propanol to clean off the dirt on the inside of the pump head and on the fitting section of the plunger seal.</li> <li>Using wiping paper to hold the plunger section (A), fit the plunger to the measuring pump main unit (B) and finger-tighten lightly.</li> <li>Push the plunger tool (A) onto the plunger (B) and tighten the plunger ASSY.</li> <li>Press [Plunger Operation]. The measuring pump plunger moves toward the back of the instrument.</li> <li>Slowly push the seal holder (B) with the alignment mark (a) facing up onto the plunger (A). Then, carefully push the pump head (C) onto the seal holder horizontally.</li> <li>Attach the 2 hexagon socket screws (B) to the pump head (A), and tighten with an M4 Allen wrench.</li> <li>Reattach the tubing on the top and bottom of the pump head and on port 5 and the center port of the low-pressure valve to their original positions, and fix them using a 6 mm and 8 mm wrench.</li> <li>Close the valve mount door.</li> <li>Press [Next]. The next screen is displayed.</li> <li>Press [Exit]. After finishing, perform a purge and check for liquid leakage from any gap between the pump head and the head holder or the tubing connection parts. The replacement history is recorded in the maintenance log.</li></ol> <h3>Related Notes</h3><ul><li>Be careful not to break the plunger when pushing the plunger tool onto the plunger.</li> <li>Accuracy of the measurements may deteriorate if there is seal residue or other matter adhering to the plunger.</li> <li>The plunger may break if the pump head is pushed in with excessive force.</li> <li>Tighten the right and left hexagon socket screws alternately 90° each, using the longer side of the M4 Allen wrench as a handle to firmly tighten them at the end.</li></ul> <h3>Necessary Parts</h3>Measuring Pump Plunger Replacement Part 228-35010-91 Qty: 1 Visit our <a href="https://store.shimadzu.com/" target="_blank">webstore</a> to order parts. <h3>Necessary Tools</h3><ul> <li>6mm Wrench</li> <li>8mm Wrench</li> <li>M4 Allen Wrench</li> <li>Plunger Tool 228-34672-02</li> </ul> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Replacing the Measuring Pump Plunger Seal - LC-2040</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/MWKPgmZT0zU?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Warnings</h3> <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>Keep the pump head level and slowly pull it out. The plunger may break if it is pulled out at an angle.</strong> <h3>Procedures</h3><ol><li>Press [Plunger Seal] on the [Replacement Parts Selection] screen. The measuring pump plunger moves all the way back, and the [Measuring pump plunger seal] screen is displayed.</li> <li>Open the valve mount door.</li> <li>Use a wrench (6 mm width/8 mm width) to loosen the 3 male nuts (A).</li> <li>Remove the flow line tubing from the top and bottom of the pump head and from port 5 and the center port of the low- pressure valve.</li> <li>Use an M4 Allen wrench to remove the 2 hexagon socket screws (B) located on the pump head (A).</li> <li>Keep the pump head (A) level and slowly pull it away from the seal holder (B).</li> <li>Keep the seal holder (A) level and slowly pull it away from the plunger (B).</li> <li>Vertically insert the end of the seal tool (A) with a stepped side (a) into the plunger seal (B).</li> <li>Pull out the seal tool (A) vertically to remove the plunger seal (B) from the seal holder (C).</li> <li>If the inside (a) of the pump head or the fitting section (b) of the plunger seal is dirty use wiping paper moistened with 2- propanol to wipe clean.</li> <li>Place a new plunger seal (B) on the seal holder (C). Vertically insert the non-stepped end (a) of the seal tool (A) into the plunger seal (B), and push down until it is level.</li> <li>Pull out the seal tool (A) vertically.</li> <li>Slowly push the seal holder (B) with the alignment mark (a) facing up onto the plunger (A). Then, carefully push the pump head (C) onto the seal holder horizontally.</li> <li>Attach the 2 hexagon socket screws (B) to the pump head (A), and tighten with an M4 Allen wrench.</li> <li>Reattach the tubing on the top and bottom of the pump head and the tubing to port 1 of the low-pressure valve to the original positions, and fix them using a 6 mm and 8 mm wrench.</li> <li>Close the valve mount door.</li> <li>Press [Next]. The next screen is displayed.</li> <li>Press [Exit]. After finishing, perform a purge and check for liquid leakage from any gap between the pump head and the head holder or the tubing connection parts. The replacement history is recorded in the maintenance log.</li></ol> <h3>Related Notes</h3><ul> <li>Airtightness cannot be maintained if there is seal residue or other matter adhering to the pump head or the plunger seal.</li> <li>The plunger may break if the pump head is pushed in with excessive force.</li> <li>Tighten the right and left hexagon socket screws alternately 90° each, using the longer side of the M4 Allen wrench as a handle to firmly tighten them at the end.</li> <li>If liquid leakage is found even after replacing the plunger seal, the surface of the measuring pump plunger may be scratched. Inspect the plunger, and if the measuring pump plunger is scratched, replace with a new plunger.</li> </ul> <h3>Necessary Parts</h3>Measuring Pump Plunger Seal Consumable Part 228-35145 Qty: 1 Visit our <a href="https://store.shimadzu.com/" target="_blank">webstore</a> to order parts. <h3>Necessary Tools</h3><ul> <li>10mm Wrench</li> <li>8mm Wrench</li> <li>M4 Allen Wrench</li> <li>Seal Tool S228-25142-01</li> </ul> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Replacing the Needle - Part 1</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/4-ewnk5WAlM?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Warnings</h3> <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>Insert the needle until it touches the back of the connection hole, and then tighten using a wrench. If the needle is not fully inserted and does not reach the back of the connection hole, a dead volume will be created and may cause a broad peak or cross contamination. Tighten the male nut fixing the needle 180°. Also, be sure to use the provided Ferrule 1.2F. Using a regular ferrule (Ferrule 1.6F) may cause liquid leakage.</strong> <h3>Procedures</h3><ol><li>Press [Needle] on the [Replacement Parts Selection] screen.</li> <li>Press [Power Off]. Press [OK]. The needle moves to the center of the instrument and the power is turned OFF.</li> <li>[[Remove the Front Panel->Removing the Front Panel (291)]]</li> <li>Open the clamp (B) on the left side of the Z mount cover, and remove the flat cable (C) from the Z mount cover.</li> <li>Hold both sides of the Z mount cover bottom (a), and pull forward to remove the cover.</li> <li>Use a 6 mm wrench to loosen the male nut (A) and remove the needle (B).</li> <li>Attach the male nut (B) and the ferrule (C) to the new needle (A).</li> <li>Firmly finger-tighten the nut, and use a wrench to tighten it 180°.</li> <li>Align the protrusion (a) on the top of the Z mount with the slit (b) on the top of the Z mount cover and fit them together.</li> <li>Hold the bottom of the Z mount from both sides, and fit the right and left catches (c) on the bottom of the Z mount cover to the Z mount bottom.</li> <li>Pass the flat cable (A) through the clamp (B) and close the clamp.</li> </ol> Please see [[Replacing the Needle - Part 2->Replacing the Needle - Part 2 (295)]] to complete this maintenance procedure. <h3>Related Notes</h3><ul> <li>The [Replacing the Needle] screen can also be displayed by pressing [Needle] on the replacement parts tab in the [Maintenance] screen.</li> <li>If the clamp (B) is stiff and difficult to open, hold the Z mount cover (A) with one hand and open the clamp (B).</li> </ul> <h3>Necessary Parts</h3>Needle Consumable Part 228-41024-99 Qty: 1 Remarks: The needle base is covered with a green tube for an identification mark. A ferrule and male nut are provided. Visit our <a href="https://store.shimadzu.com/" target="_blank">webstore</a> to order parts. <h3>Necessary Tools</h3>6mm Wrench <h3>Related Procedures</h3> <span class="btn-link">[[Attaching the Front Panel->Attaching the Front Panel (292)]]</span> <span class="btn-link">[[Displaying the Replaced Part Items->Displaying the Replaced Part Items (351)]]</span> <span class="btn-link">[[Removing the Front Panel->Removing the Front Panel (291)]]</span> <span class="btn-link">[[Replacing the Needle - Part 2->Replacing the Needle - Part 2 (295)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Replacing the Needle - Part 2</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/6bHWyli-Eus?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Warnings</h3> <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>After inspection and replacement, be sure to attach the Z mount cover and clamp the flat cable. Failure to do so may result in the cable interfering with another internal part and causing abnormal operation or a malfunction.</strong> <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>Never put hands inside the instrument during autosampler operation. Failure to comply with the above precaution may result in injury from the needle. During checking and adjustment of the needle position, operation continues even when the front panel is open.</strong> <h3>Procedures</h3> If you have not already done so, please see [[Replacing the Needle - Part 1->Replacing the Needle - Part 1 (294)]] before viewing this maintenance procedure. <ol><li>Press (Power button) to turn ON.</li> <li>After the instrument starts, input the login pass ID Number and press [OK] to display the [Replacing the Needle] screen.</li> <li>Press the [Next] button. The usage value is reset. The replacement history is recorded in the maintenance log.</li> <li>Check that the usage value is reset and then press the [Next] button.</li> <li>Press [Start] to check that the needle (A) and the injection port (B) are correctly aligned.</li> <li>At the position where the tip of the needle seals the needle seal, perform an up-and-down movement of about 2 mm 2 times.</li> <li>At this time, check that the needle has moved directly down to the injection port without moving left, right, forward, or backward.</li> <li>Press [Next] then [[Attach the Front Panel->Attaching the Front Panel (292)]]</li> <li>Press [Start]. Conditioning is performed.</li> <li>Press [Exit].</li> </ol> <h3>Related Notes</h3><ul> <li>If the needle position is out of alignment, press [Stop] to cancel the task, and perform [Inj. Port Adjustment] for calibration.</li> <li>When the same needle is reattached during maintenance or other tasks, if cross contamination increases, replace with a new needle.</li> </ul> <h3>Necessary Parts</h3>Needle Consumable Part 228-41024-99 Qty: 1 Remarks: The needle base is covered with a green tube for an identification mark. A ferrule and male nut are provided. Visit our <a href="https://store.shimadzu.com/" target="_blank">webstore</a> to order parts. <h3>Necessary Tools</h3>6mm Wrench <h3>Related Procedures</h3> <span class="btn-link">[[Attaching the Front Panel->Attaching the Front Panel (292)]]</span> <span class="btn-link">[[Displaying the Replaced Part Items->Displaying the Replaced Part Items (351)]]</span> <span class="btn-link">[[Removing the Front Panel->Removing the Front Panel (291)]]</span> <span class="btn-link">[[Replacing the Needle - Part 1->Replacing the Needle - Part 1 (294)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Replacing the Needle Seal</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/Fas1R2IIC4E?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Warnings</h3> <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>If it is difficult to remove the needle seal, do NOT use excessive force to remove it. Applying excessive energy may result in injury. If the needle seal cannot be removed, replace the needle seal and the housing at the same time.</strong> <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>When inserting the needle seal, use clean wiping paper to hold the needle seal while taking care to keep the hole dust- and dirt-free. Dust or dirt creates a microscopic gap that may cause carryover or liquid leakage.</strong> <h3>Procedures</h3><ol><li>Press [Needle Seal] on the [[ [Replacement Parts Selection] screen.->Displaying the Replaced Part Items (351)]]</li> <li>Press [Power OFF]. When the confirmation screen opens, Press [OK]. The needle moves to the center of the instrument and the power is turned OFF.</li> <li>[[Remove the front panel->Removing the Front Panel (291)]]</li> <li>Use a 10 mm wrench (B) to remove the cap (A).</li> <li>The needle seal is now exposed. Use the needle seal tool (B) to loosen and remove the housing (A).</li> <li>Push the bottom of the needle seal (A) to remove the needle seal from the housing (B).</li> <li>Attach the housing (A) to the high-pressure valve, and use the needle seal tool (B) to tighten it.</li> <li>Insert a new needle seal (A) into the housing (B).</li> <li>Firmly finger-tighten the cap (A) to the housing, and then use a 10 mm wrench (B) to tighten and turn the cap 90°.</li> <li>[[Attach the front panel.->Attaching the Front Panel (292)]]</li> <li>Press (Power button) to turn ON. After the instrument starts, input the login pass ID Number and press [OK].</li> <li>Press the [Next] button. The usage value is reset. The replacement history is recorded in the maintenance log.</li> <li>Check that the usage value is reset and then press the [Next] button.</li> <li>Press [Start]. Conditioning is performed.</li> <li>Press [Exit]. Press [Exit] again.</li> </ol> <h3>Related Notes</h3>The [Replacing the Needle Seal] screen can also be displayed by pressing [Needle Seal] on the replacement parts tab in the [Maintenance] screen. <h3>Necessary Parts</h3> Housing, Needle Seal XR Replacement Part S228-50391 Qty: 1 Needle Seal XR Consumable Part S228-50390 Qty: 1 Needle Seal XR Kit (Containing 3 Needle Seals) Consumable Part S228-50452-93 Qty: 1 Remarks: The set includes 1 housing and 3 needle seals Visit our <a href="https://store.shimadzu.com/" target="_blank">webstore</a> to order parts. <h3>Necessary Tools</h3><ul> <li>10mm Wrench</li> <li>Needle Seal XR Tool S228-50570</li></ul> <h3>Related Procedures</h3> <span class="btn-link">[[Attaching the Front Panel->Attaching the Front Panel (292)]]</span> <span class="btn-link">[[Displaying the Replaced Part Items->Displaying the Replaced Part Items (351)]]</span> <span class="btn-link">[[Removing the Front Panel->Removing the Front Panel (291)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Displaying the Replaced Part Items</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/SZsqFrwjkog?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Procedures</h3><ol><li>Press (Menu). The [Menu] screen opens.</li> <li>Press [Maintenance]. The [Maintenance] screen is displayed. The tab for the replaced part is displayed first.</li> <li>Press the button to select each unit. The [Replacement Parts Selection] screen is displayed. The tab for the selected unit is displayed.</li> <li>Press the button with the corresponding name to display the replacement screen for a main part.</li> <li>Press an item of the part for replacement.</li> <li>Carry out the operation by following the explanation in the screen. Operations vary depending on each item.</li> </ol> <h3>Related Notes</h3><ul> <li>Press (Replacement part) to display the tab for the replaced part when other tabs are displayed in the [Maintenance] screen.</li> <li>When a service duration for a part in the unit exceeds the replacement guidelines settings, (Warning!) is displayed.</li> <li>If a W lamp is connected, press [D2 Lamp / W Lamp] once to alternatively switch the [D2 Lamp] and [W Lamp] displays. Pressing the button displayed as "Usage frequency / Replacement guidelines" displays the replacement screen for the corresponding part.</li> </ul> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Replacing the Outlet Tubing</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/8AsVmMMECoE" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Warnings</h3> <img class="warning" style="float: left; margin-bottom:25px;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>Do NOT crush or bend tubing at a sharp angle using pliers, etc. When bending stainless steel tubing, if the circular arc of the bend (bend radius) is small, the inside shape of the tubing will become deformed, which may cause a blockage in the tubing or an increase in pressure. The performance of the instrument or the sample loop life is also significantly affected.</strong> <img class="warning" style="float: left; margin-bottom:25px;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>Do NOT frequently bend and straighten in the same place. If the tubing is repeatedly bent in the same place, the tubing will weaken, which may cause a rupture or cracks resulting in leakage of solvent. The performance of the instrument or the sample loop life is also significantly affected.</strong> <h3>Procedures</h3><ol><li>Perform "Z-Home" to move the needle to the center.</li> <li>Press (Power button) to turn OFF.</li> <li>Remove the front panel.</li> <li>Remove the column side of the outlet tubing from the column.</li> <li>Use a 6 mm wrench to remove the nut (A) of the outlet tubing connected to port 5 of the high-pressure valve.</li> <li>Open the clamp inside the column oven and remove the outlet tubing.</li> <li>Use a 6 mm wrench to attach new outlet tubing to port 5 while ensuring that the outlet tubing passes through the notch (A) of the plastic cover located between the port and the oven.</li> <li>Return and fix the outlet tubing to the clamp inside the column oven as it originally was.</li> <li>Connect the end of the outlet tubing on the column oven side to the column.</li> <li>Attach the front panel.</li> <li>Press (Power button) to turn ON.</li> <li>Record the replacement history in the maintenance log.</li> </ol> <h3>Related Notes</h3><ul> <li>When replacing clogged outlet tubing, it is recommended to first attempt to remove the blockage by reverse rinsing of the flow line. After reverse rinsing, if the tubing is still clogged, follow the procedure below to replace with new tubing.</li> <li>The shape of the tubing connection part may differ depending on the column manufacturer or column type. After the column is replaced, if cross contamination increases due to any shape difference, or if the peak shape is affected, replace with new outlet tubing.</li> <li>SUS pipe 0.1 x 600 OUT is a flexible pipe that is easily maneuvered. In addition, the included fitting is a reusable UHPLC fitting. For information on how to use the UHPLC fitting, refer to the included instruction manual. </li> </ul> <h3>Necessary Parts</h3>SUS Pipe 0.1 x 600 OUT (for LC-2040) Consumable Part 228-53184-91 Qty: 1 SUS Pipe 0.3 x 600 OUT (for LC-2030) Consumable Part 228-56217-44 Qty: 1 Visit our <a href="https://store.shimadzu.com/" target="_blank">webstore</a> to order parts. <h3>Necessary Tools</h3>6mm Wrench <h3>Related Procedures</h3> <span class="btn-link">[[Attaching the Front Panel->Attaching the Front Panel (292)]]</span> <span class="btn-link">[[Recording Replacement History in the Replacement Log->Recording Replacement History in the Replacement Log (352)]]</span> <span class="btn-link">[[Removing the Front Panel->Removing the Front Panel (291)]]</span> <span class="btn-link">[[Rinsing the Flow Line->Rinsing the Flow Line (311)]]</span> <span class="btn-link">[[Z-Home->Z-Home (358)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Replacing the Rack Panel</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/rTzFyLV_qVI?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Procedures</h3><ol><li>Remove the sample racks (A).</li> <li>Use a Phillips screwdriver to remove the 2 screws (C) that secure the rack panel (B) and the L-shaped sheet metal part.</li> <li>Then remove the rack panel (B).</li> <li>Use the 2 removed screws (C) to fit a new rack panel (B).</li> <li>Return the sample racks to the original positions.</li> <li>[[Record the replacement history in the maintenance log.->Recording Replacement History in the Replacement Log (352)]]</li> </ol> <h3>Related Notes</h3>If the replaced sample racks interfere with the front panel, finely adjust the rack panel to the left or right. <h3>Necessary Parts</h3>Rack Panel Maintenance Part 228-56268-41 Qty: 1 Visit our <a href="https://store.shimadzu.com/" target="_blank">webstore</a> to order parts. <h3>Necessary Tools</h3>#2 Phillips Screwdriver <h3>Related Procedures</h3> <span class="btn-link">[[Recording Replacement History in the Replacement Log->Recording Replacement History in the Replacement Log (352)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Replacing the Rinsing Port Cap</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/AfcjUajXtaE?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Warnings</h3> <img class="warning" style="float: left; " src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>Insert the cap all the way. If it is raised, it may come into contact with the Z mount.</strong> <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>Press the cover all the way down. If it is raised, it may come into contact with the Z mount.</strong> <h3>Procedures</h3><ol><li>[[Perform "Z-Home"->Z-Home (358)]] to move the needle to the center.</li> <li>Press (Power button) to turn OFF.</li> <li>[[Remove the front panel.->Removing the Front Panel (291)]]</li> <li>Remove the rinsing port cover (A) from the rinsing port (B).</li> <li>Remove the cap (B) fitted to the rear of the rinsing port cover (A).</li> <li>Fit a new cap (C) onto the rinsing port cover (A).</li> <li>Reattach the rinsing port cover (A).</li> <li>Lightly move the Z mount (A) by hand and check that it does not interfere with the rinsing port cover (B).</li> <li>[[Attach the front panel.->Attaching the Front Panel (292)]]</li> <li>Press (Power button) to turn ON.</li> <li>[[Record the replacement history in the maintenance log.->Recording Replacement History in the Replacement Log (352)]]</li> </ol> <h3>Related Notes</h3>Because the front cap is not used, there is no need to replace it. <h3>Necessary Parts</h3>Rinsing Port Cap (No Hole), 10 Caps Consumable Part 228-48331-91 Qty: 10 Remarks: For rinse solution containing a highly volatile acid (e.g. formic acid, TFA, acetic acid). Rinsing Port Cap (with Hole), 10 Caps Consumable Part 228-48331-92 Qty: 10 Remarks: For anything other than the above (However, if cross contamination is a problem even with the above rinse solution, use the rinsing port cap with hole.) Visit our <a href="https://store.shimadzu.com/" target="_blank">webstore</a> to order parts. <h3>Related Procedures</h3> <span class="btn-link">[[Attaching the Front Panel->Attaching the Front Panel (292)]]</span> <span class="btn-link">[[Recording Replacement History in the Replacement Log->Recording Replacement History in the Replacement Log (352)]]</span> <span class="btn-link">[[Removing the Front Panel->Removing the Front Panel (291)]]</span> <span class="btn-link">[[Z-Home->Z-Home (358)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Z-Home</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/lqHjWXTs5k8?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Procedures</h3><ol><li>Press [Z-Home] on the [Auxiliary Operation] screen. The [Z-Home] screen is displayed.</li> <li>Press [Z-Home] or [Standby Position] to move the needle position.</li> <li>Z-Home: The needle moves up to the highest point and moves to the center of the instrument (Z-Home).</li> <li>Standby position: The needle moves to the start position (standbyposition).</li> <li>Press [Close].</li> </ol> <h3>Related Procedures</h3> <span class="btn-link">[[Displaying Auxiliary Function Items->Displaying Auxiliary Function Items (357)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Replacing the Sample Loop - Part 1</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/DTJNaS8COzI?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Warnings</h3><img class="warning" style="float: left; margin-bottom: 25px; " src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>Do NOT crush or bend tubing at a sharp angle using pliers, etc. When bending stainless steel tubing, if the circular arc of the bend (bend radius) is small, the inside shape of the tubing will become deformed, which may cause a blockage in the tubing or an increase in pressure. The performance of the instrument or the sample loop life is also significantly affected.</strong> <img class="warning" style="float: left; margin-bottom: 25px;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>Do NOT frequently bend and straighten in the same place. If the tubing is repeatedly bent in the same place, the tubing will weaken, which may cause a rupture or cracks resulting in leakage of solvent. The performance of the instrument or the sample loop life is also significantly affected.</strong> <h3>Procedures</h3><ol><li>Press [Sample Loop] on the [Replacement Parts Selection] screen.</li> <li>Press [Power OFF]. Press [OK]. The needle moves to the center of the instrument and the power is turned OFF.</li> <li>[[Remove the Front Panel->Removing the Front Panel (291)]]</li> <li>Use a 6 mm wrench to remove the male nut (A) that fixes the sample loop to port 1 of the high-pressure valve.</li> <li>Open the clamp (B) on the left side of the Z mount cover (A), and remove the flat cable (C) from the Z mount cover.</li> <li>Hold both sides of the Z mount cover bottom (a), and pull forward to remove the cover.</li> <li>Use a 6 mm wrench to remove the male nut (A) that fixes the sample loop to the needle.</li> <li>Remove the sample loop from the 2 clamps (a) attached to the inner front of the plastic cover.</li> <li>Remove the sample loop from the 3 hooks (b) attached to the inner left side of the plastic cover.</li> <li>Remove the sample loop from the rectangular socket holder (c) located at the rear of the Z mount.</li> <li>To remove the sample loop from the instrument, pass the section of the sample loop that was connected to the high-pressure valve from the front to the back through the gap (a)</li> <li>Between the upper left of the XY arm and the plastic cover, then from the back to the front through the gap (b) around the rectangular socket holder at the rear of the Z mount.</li> <li>Proceed to [[Replacing the Sample Loop - Part 2->Replacing the Sample Loop - Part 2 (299)]]</li> </ol> <h3>Related Notes</h3><ul> <li>The [Replacing the Sample Loop] screen can also be displayed by pressing [Sample Loop] on the replacement parts tab in the [Maintenance] screen.</li> <li>If the clamp (B) is stiff and difficult to open, hold the Z mount cover (A) with one hand and open the clamp (B).</li> </ul> <h3>Necessary Parts</h3>Sample Loop for 100 µL Injection (for LC-2030) Consumable Part 228-56074-42 Qty: 1 Remarks: This is the sample loop for 100 μL injections. Sample Loop for 50 μL Injection (for LC-2040) Consumable Part 228-56074-44 Qty: 1 Remarks: This is the sample loop for 50 μL injections. Visit our <a href="https://store.shimadzu.com/" target="_blank">webstore</a> to order parts. <h3>Necessary Tools</h3>6mm Wrench <h3>Related Procedures</h3> <span class="btn-link">[[Attaching the Front Panel->Attaching the Front Panel (292)]]</span> <span class="btn-link">[[Removing the Front Panel->Removing the Front Panel (291)]]</span> <span class="btn-link">[[Replacing the Sample Loop - Part 2->Replacing the Sample Loop - Part 2 (299)]]</span> <span class="btn-link">[[Replacing the Sample Loop - Part 3->Replacing the Sample Loop - Part 3 (300)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Replacing the Sample Loop - Part 2</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/uofdfceuWFs?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Warnings</h3><img class="warning" style="float: left; margin-bottom: 25px; " src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>After inspection and replacement, be sure to attach the Z mount cover and clamp the flat cable. Failure to do so may result in the cable interfering with another internal part and causing abnormal operation or a malfunction.</strong> <img class="warning" style="float: left; margin-bottom: 25px;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>Insert the sample loop until it touches the back of the connection hole, and then use a wrench to tighten it. If the sample loop is not fully inserted and does not reach the back of the connection hole, a dead volume will be created and may cause a broad peak or cross contamination.</strong> <h3>Procedures</h3>If you have not already done so, please see [[Replacing the Sample Loop - Part 1->Replacing the Sample Loop - Part 1 (276)]] <ol><li>In the reverse order of removing the old sample loop, pass the new sample loop from the front to the back</li> <li>Through the gap (b) around the rectangular socket holder at the rear of the Z mount,</li> <li>and then from the back to the front through the gap (a) between the upper left of the XY arm and the plastic cover.</li> <li>Attach the sample loop (A) to the rectangular socket holder (B) at the rear of the Z mount.</li> <li>Fit the male nut and the ferrule to the sample loop, then use a 6 mm wrench to tighten the male nut (A)</li> <li>and fit the sample loop to the joint section on the needle side.</li> <li>Attach the section (a) of the sample loop that is positioned by bands to the innermost hook (b) attached to the inner left side of the plastic cover.</li> <li>Fix the sample loop to the remaining 2 hooks (b).</li> <li>Fix the sample loop to the 2 clamps (a) attached on the inside of the plastic cover.</li> <li>Align the protrusion (a) on the top of the Z mount with the slit (b) on the top of the Z mount cover and fit them together.</li> <li>Hold the bottom of the Z mount from both sides, and fit the right and left catches (c) on the bottom of the Z mount cover to the Z mount bottom.</li> <li>Pass the flat cable (A) through the clamp (B) and close the clamp.</li> <li>Proceed to [[Replacing the Sample Loop - Part 3->Replacing the Sample Loop - Part 3 (300)]]</li> </ol> <h3>Related Notes</h3><ul> <li>As shown in the diagram, to ensure the band heads face toward the left side of the instrument, twist about 90° counterclockwise when seen from the front of the instrument, and attach the sample loop to the hook.</li> <li>If the clamp (B) is stiff and difficult to close, hold the Z mount cover with one hand and close the clamp (B).</li> </ul> <h3>Necessary Parts</h3>Extended Sample Loop Kit for 2 mL Injection Optional Part 228-45405-42 Qty: 1 Remarks: This is the extended sample loop kit for 2 mL injections. For installation, refer to the instruction manual provided with the part. Extended Sample Loop Kit for 500 μL Injection Optional Part S228-45405-41 Qty: 1 Remarks: This is the extended sample loop kit for 500 μL injections. For installation, refer to the instruction manual provided with the part. Sample Loop for 100 µL Injection (for LC-2030) Consumable Part 228-56074-42 Qty: 1 Remarks: This is the sample loop for 100 μL injections. Sample Loop for 50 μL Injection (for LC-2040) Consumable Part 228-56074-44 Qty: 1 Remarks: This is the sample loop for 50 μL injections. Visit our <a href="https://store.shimadzu.com/" target="_blank">webstore</a> to order parts. <h3>Necessary Tools</h3>6mm Wrench <h3>Related Procedures</h3><span class="btn-link">[[Attaching the Front Panel->Attaching the Front Panel (292)]]</span> <span class="btn-link">[[Removing the Front Panel->Removing the Front Panel (291)]]</span> <span class="btn-link">[[Replacing the Sample Loop - Part 1->Replacing the Sample Loop - Part 1 (276)]]</span> <span class="btn-link">[[Replacing the Sample Loop - Part 3->Replacing the Sample Loop - Part 3 (300)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Replacing the Sample Loop - Part 3</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/UPCwamNgYx4?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Procedures</h3>If you have not already done so, please see [[Replacing the Sample Loop - Part 1->Replacing the Sample Loop - Part 1 (276)]] and [[Replacing the Sample Loop - Part 2->Replacing the Sample Loop - Part 2 (299)]] <ol><li>Fit the male nut and the ferrule to the sample loop, then use a 6 mm wrench to tighten the male nut (A) and fit the sample loop to port 1 of the high-pressure valve.</li> <li>Slacken the sample loop fitted to port 1 as shown, then adjust the tubing configuration so that the lowest point is positioned above the high-pressure valve tray.</li> <li>Move the Z mount by hand toward the upper part of the high-pressure valve </li> <li>Check that the sample loop does not interfere with the Z mount bottom.</li> <li>[[Attach the Front Panel->Attaching the Front Panel (292)]]</li> <li>Press (Power button) to turn ON. After the instrument starts, input the login pass ID Number and press [OK] to display the [Replacing the Sample Loop] screen.</li> <li>Press the [Next] button. The next screen is displayed. Check that the usage value is reset and press [OK]</li> <li>Enter the volume of the fitted sample loop, and then press [Exit].</li> </ol> <h3>Related Notes</h3>When replacing with a sample loop for 50 μL or below, if the sample aspiration rate is set to a value greater than 5.0 μL/sec, the sample aspiration rate will be automatically changed to 5.0 μL/sec. <h3>Necessary Parts</h3>Extended Sample Loop Kit for 2 mL Injection Optional Part 228-45405-42 Qty: 1 Remarks: This is the extended sample loop kit for 2 mL injections. For installation, refer to the instruction manual provided with the part. Extended Sample Loop Kit for 500 μL Injection Optional Part S228-45405-41 Qty: 1 Remarks: This is the extended sample loop kit for 500 μL injections. For installation, refer to the instruction manual provided with the part. Sample Loop for 100 µL Injection (for LC-2030) Consumable Part 228-56074-42 Qty: 1 Remarks: This is the sample loop for 100 μL injections. Sample Loop for 50 μL Injection (for LC-2040) Consumable Part 228-56074-44 Qty: 1 Remarks: This is the sample loop for 50 μL injections. Visit our <a href="https://store.shimadzu.com/" target="_blank">webstore</a> to order parts. <h3>Necessary Tools</h3>6mm Wrench <h3>Related Procedures</h3><span class="btn-link">[[Attaching the Front Panel->Attaching the Front Panel (292)]]</span> <span class="btn-link">[[Removing the Front Panel->Removing the Front Panel (291)]]</span> <span class="btn-link">[[Replacing the Sample Loop - Part 1->Replacing the Sample Loop - Part 1 (276)]]</span> <span class="btn-link">[[Replacing the Sample Loop - Part 2->Replacing the Sample Loop - Part 2 (299)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Replacing the Wiper</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/OXH_KvKy2ew?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Procedures</h3><ol><li>Press (Power button) to turn OFF.</li> <li>[[Remove the Front Panel->Removing the Front Panel (291)]]</li> <li>Slide and remove the 4 wipers (A).</li> <li>Slide and attach the 4 wipers (A).</li> <li>[[Attach the Front Panel->Attaching the Front Panel (292)]]</li> <li>Press (Power button) to turn ON.</li> <li>[[Record the replacement history in the maintenance log->Recording Replacement History in the Replacement Log (352)]]</li> </ol> <h3>Necessary Parts</h3>Wiper Rack Maintenance Part 228-57561-41 Qty: 4 Remarks: Four racks Visit our <a href="https://store.shimadzu.com/" target="_blank">webstore</a> to order parts. <h3>Related Procedures</h3><span class="btn-link">[[Attaching the Front Panel->Attaching the Front Panel (292)]]</span> <span class="btn-link">[[Removing the Front Panel->Removing the Front Panel (291)]]</span> <span class="btn-link">[[Recording the replacement history in the maintenance log->Recording Replacement History in the Replacement Log (352)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Rinsing the Flow Line</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/RZ7IFQ3ansE?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Procedures</h3><ol><li>Perform an autopurge.</li> <li> If a column is connected, remove it and install the provided PEEK tubing (or another replacement tube) and the coupling instead.</li> <li>Press [Purge] on the Main Analysis screen.</li> <li>Turn ON [1st] to [4th], and [Rinse].</li> <li>Press [Start].</li> <li>After the purge finishes, press [Close].</li> <li>Set the flow rate to 1 mL/min and press [Pump] to start pumping.</li> <li>Press [Z-Home], [Auxilliary Operation], [Z-Home], [Z-Home] again, and [Close].</li> <li>Press (Power button) to turn OFF.</li> <li>Remove the front panel.</li> <li>Remove the inlet and outlet tubing on the high-pressure valve side of the instrument as well as the tubing located on the column side.</li> <li>Connect the inlet tubing to port 5 of the high-pressure valve.</li> <li>Connect the side of the outlet tubing that was originally connected to the column to port 6 of the high-pressure valve.</li> <li>Use a beaker or similar vessel to collect the mobile phase coming from the other end of the outlet tubing.</li> <li>Press (Power button) to turn ON.</li> <li>Perform pumping of 2-propanol from the solvent delivery module at 2 mL/min to 5 mL/min.</li> <li>Return the tubing to the original positions.</li> <li>Attach the front panel.</li> <li>Reinstall the column.</li> </ol> <h3>Related Notes</h3><ul> <li>Perform reverse rinsing of the flow line if the inside of the needle is still clogged after performing the autopurge.</li> <li>Replace the needle if the needle is still clogged after reverse rinsing of the flow line.</li> </ul> <h3>Related Procedures</h3><span class="btn-link">[[Attaching the Front Panel->Attaching the Front Panel (292)]]</span> <span class="btn-link">[[Removing the Front Panel->Removing the Front Panel (291)]]</span> <span class="btn-link">[[Replacing the Needle - Part 1->Replacing the Needle - Part 1 (294)]]</span> <span class="btn-link">[[Replacing the Needle - Part 2->Replacing the Needle - Part 2 (295)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Inspecting the High-Pressure Valve Rotor and High-Pressure Valve Stator - LC-2030</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/bjuwp3wUZ9U?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Procedures</h3><ol> <li>Fill the mobile phase A flow line with an organic solvent such as 2-propanol, methanol, or acetonitrile.</li> <li>Press [HPV Rotor] or [HPV Stator] on the [[ [Replacement Parts Selection] screen->Displaying the Replaced Part Items (351)]].</li> <li>Press [Power OFF]. Press [OK]. The needle moves to the center of the instrument and the power is turned OFF.</li> </ol> <h3>Related Notes</h3><ul> <li>After the high-pressure valve rotor and stator have been replaced, perform conditioning by pumping of the mobile phase A in order to create a smooth seal surface (switching back and forth 50 times).</li> <li>The [High pressure valve rotor and stator replacement] screen can also be displayed by pressing [HPV Rotor] or [HPV Stator] on the replacement parts tab in the [Maintenance] screen.</li> </ul> <h3>Related Procedures</h3><span class="btn-link">[[Displaying the Replaced Part Items->Displaying the Replaced Part Items (351)]]</span> <span class="btn-link">[[Replacing the High-Pressure Valve Rotor and High-Pressure Valve Stator - Part 1 - LC-2030->Replacing the High-Pressure Valve Rotor and High-Pressure Valve Stator - Part 1 - LC-2030 (297)]]</span> <span class="btn-link">[[Replacing the High-Pressure Valve Rotor and High-Pressure Valve Stator - Part 2 - LC-2030 ->Replacing the High-Pressure Valve Rotor and High-Pressure Valve Stator - Part 2 - LC-2030 (298)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Replacing the High-Pressure Valve Rotor and High-Pressure Valve Stator - Part 1 - LC-2030</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/Yp3XOKpi6wU?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Warnings</h3> <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>Be sure to attach the backup ring. The backup ring is fitted to prevent the stator and the gasket from becoming tilted. If not attached, it may cause liquid leakage or wear and tear on the rotor and stator in a short period of time. </strong> <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>Be sure to remove the ring and the stator ASSY together. If trying to remove only the stator ASSY, the ring may drop, resulting in damage or injury.</strong> <h3>Procedures</h3><ol><li>[[Removing the Front Panel->Removing the Front Panel (291)]]</li> <li>Use a 6 mm wrench to loosen all male nuts (A) of the flow line tubing and remove the tubing from the high-pressure valve.</li> <li>Use an M4 Allen wrench to loosen the 3 hexagon socket screws in the order of A, B, and C by turning each screw 60°.</li> <li>Hold the high-pressure valve stator ASSY (A) and the ring (B), and remove the ring taking care not to drop it.</li> <li>Hold the outside (a) of the high pressure valve rotor (A) and remove the rotor.</li> <li>Check that the rotating and sliding surfaces (a, b) of the high pressure valve stator (A) and the high-pressure valve rotor (B) have no scratches.</li> <li>Place the high-pressure valve rotor (A) into a beaker filled with 2-propanol.</li> <li>Remove the cap (B) to disassemble the high-pressure valve stator ASSY into the high pressure valve stator (C), gasket (D), backup ring (E), and housing (F).</li> <li>Clean the high pressure valve rotor, high pressure valve stator, gasket, and housing in the beaker filled with 2-propanol for 5 minutes in an ultrasonic bath.</li> <li>Align the notch (a) on the high pressure valve rotor (A) with the alignment mark (b) of the shaft (B) and gently push and fit them together.</li> <li>Attach the ring (B) to the high pressure valve main unit (A).</li> <li>Reassemble the components onto the housing (A) in the following order-- backup ring (B), gasket (C), and high-pressure valve stator (D).</li> <li>Fit the cap (E) to complete reassembly of the high-pressure valve stator ASSY.</li> <li>Use wiping paper moistened with 2-propanol to wipe the sliding surface (a) of the high-pressure valve stator.</li> <li>Proceed to [[Replacing the High-Pressure Valve Rotor and High-Pressure Valve Stator - Part 2 - LC-2030 ->Replacing the High-Pressure Valve Rotor and High-Pressure Valve Stator - Part 2 - LC-2030 (298)]]</li> </ol> <h3>Related Notes</h3><ul> <li>There is no need to remove the needle seal.</li> <li>If only certain screws are loosened, the stator may become tilted, making it difficult or impossible to remove the stator or resulting in a seized screw.</li> <li>If a scratch is found through visual inspection, replace with a new high-pressure valve rotor and highpressure valve stator. Any scratches can cause wear and tear in a short period of time.</li> <li>As much as possible, touch only the very edge of the highpressure valve rotor. After fitting, use wiping paper moistened with 2- propanol to wipe the rotor sliding surface that contacts the sliding surface of the high-pressure valve stator.</li> </ul> <h3>Necessary Parts</h3>High-Pressure Valve Gasket Replacement Part 228-56061 Qty: 1 Remarks: When using solvents such as tetrahydrofuran (THF), as a general guideline replace the gasket after about 100,000 injections. High-Pressure Valve Housing Replacement Part 228-56278-41 Qty: 1 Remarks: Replace the housing when the tubing connection has worn out due to repeated attaching and removing of the tubing. High-Pressure Valve Rotor (for LC-2030) Consumable Part 228-56098-00 Qty: 1 High-Pressure Valve Rotor (for LC-2040) Consumable Part 228-48854-00 Qty: 1 High-Pressure Valve Stator (for LC-2030) Consumable Part 228-56253-42 Qty: 1 High-Pressure Valve Stator (for LC-2040) Consumable Part 228-59076-41 Qty: 1 Visit our <a href="https://store.shimadzu.com/" target="_blank">webstore</a> to order parts. <h3>Necessary Tools</h3><ul> <li>6mm Wrench</li> <li>M4 Allen Wrench</li> </ul> <h3>Related Procedures</h3><span class="btn-link">[[Attaching the Front Panel->Attaching the Front Panel (292)]]</span> <span class="btn-link">[[Inspecting the High-Pressure Valve Rotor and High-Pressure Valve Stator->Inspecting the High-Pressure Valve Rotor and the High-Pressure Valve Stator - LC-2030 (296)]]</span> <span class="btn-link">[[Removing the Front Panel->Removing the Front Panel (291)]]</span> <span class="btn-link">[[Replacing the High-Pressure Valve Rotor and High-Pressure Valve Stator - Part 2 - LC-2030 ->Replacing the High-Pressure Valve Rotor and High-Pressure Valve Stator - Part 2 - LC-2030 (298)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Replacing the High-Pressure Valve Rotor and High-Pressure Valve Stator - Part 2 - LC-2030</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/ncIA2kc5zPk?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Warnings</h3> <img class="warning" style="float: left;" src="https://www.ssi.shimadzu.com/sites/ssi.shimadzu.com/files/support/virtual-advisors/virtual-advisor-warning-icon.png" alt="warning-icon"><strong>Use the longer side of the Allen wrench as a handle to further tighten. If the screws are inadequately tightened, the high-pressure valve stator ASSY may be installed at an angle and result in liquid leakage or cause wear and tear of the rotor and stator in a short period of time. </strong> <h3>Procedures</h3>If you have not already done so, please see [[Replacing the High-Pressure Valve Rotor and High-Pressure Valve Stator - Part 1 - LC-2030 ->Replacing the High-Pressure Valve Rotor and High-Pressure Valve Stator - Part 1 - LC-2030 (297)]] before viewing this maintenance procedure. <ol><li>Fit the high-pressure valve stator ASSY (A) onto the high-pressure valve main unit.</li> <li>Use a finger to lightly press the center down while ensuring the high-pressure valve stator ASSY (A) is level.</li> <li>While pressing on the high pressure valve stator ASSY (A), insert the longer side of the Allen wrench (M4) (B) into each of the 3 hexagon socket screws (C) and lightly turn to tighten until the screw head makes contact.</li> <li>Use an Allen wrench (M4) to tighten each of the 3 hexagon socket screws 60° in the order of A, B, and C.</li> <li>Loosen each of the 3 hexagon socket screws 60° from fully tightened in the order of C, B, and A 3 times. (Loosen each screw a total of 180°).</li> <li>Once again, repeatedly tighten each screw 60° in the order of A, B, and C until completely tightened.</li> <li>Reattach the flow line tubing of each high-pressure valve port to its original position, and use a 6 mm wrench to tighten the 5 male nuts (A).</li> <li>Lightly move the Z mount (A) by hand, and check that the bottom of the Z mount does not interfere with the tubing (B) connected to the high-pressure valve.</li> <li>Attach the front panel.</li> <li>Press (Power button) to turn ON. After the instrument starts, input the login pass ID Number and press [OK] to display the [High pressure valve rotor and stator replacement] screen.</li> <li>Select the replaced part and press [Next].Press OK to verify that the usage value should be reset. The next screen is displayed and the usage value is reset.</li> <li>The replacement history is recorded in the maintenance log.</li> <li>Check that the usage value is reset and then press [Next]. The next screen is displayed.</li> <li>Press [Start]. Conditioning is performed.</li> <li>After conditioning is finished, press [Exit].</li> </ol> <h3>Related Notes</h3><ul> <li>Move the Z mount by holding the metallic section.</li> <li>Because abrasion powder may be generated initially, be sure to remove the column before performing conditioning. To create a smooth seal surface between the high-pressure valve rotor and stator, perform conditioning by pumping of the mobile phase A at 1 mL/min (switching back and forth 50 times).</li> <li>Check for liquid leakage from gaps around the high-pressure valve.</li> </ul> <h3>Necessary Parts</h3>High-Pressure Valve Gasket Replacement Part 228-56061 Qty: 1 Remarks: When using solvents such as tetrahydrofuran (THF), as a general guideline replace the gasket after about 100,000 injections. High-Pressure Valve Housing Replacement Part 228-56278-41 Qty: 1 Remarks: Replace the housing when the tubing connection has worn out due to repeated attaching and removing of the tubing. High-Pressure Valve Rotor (for LC-2030) Consumable Part 228-56098-00 Qty: 1 High-Pressure Valve Stator (for LC-2030) Consumable Part 228-56253-42 Qty: 1 Visit our <a href="https://store.shimadzu.com/" target="_blank">webstore</a> to order parts. <h3>Necessary Tools</h3><ul> <li>6mm Wrench</li> <li>M4 Allen Wrench</li> </ul> <h3>Related Procedures</h3><span class="btn-link">[[Attaching the Front Panel->Attaching the Front Panel (292)]]</span> <span class="btn-link">[[Inspecting the High-Pressure Valve Rotor and High-Pressure Valve Stator->Inspecting the High-Pressure Valve Rotor and the High-Pressure Valve Stator - LC-2030 (296)]]</span> <span class="btn-link">[[Recording Replacement History in the Replacement Log->Recording Replacement History in the Replacement Log (352)]]</span> <span class="btn-link">[[Replacing the High-Pressure Valve Rotor and High-Pressure Valve Stator - Part 1 - LC-2030 ->Replacing the High-Pressure Valve Rotor and High-Pressure Valve Stator - Part 1 - LC-2030 (297)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Inspecting/Replacing the Low-Pressure Valve Rotor and Low-Pressure Valve Stator - LC-2030</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/wm9kyfS4-6o?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Procedures</h3><ol><li>Press [LPV Rotor] or [LPV Stator] on the [Replacement Parts Selection] screen. The [Low pressure valve rotor and stator replacement] screen is displayed.</li> <li>Lower the rinse solution bottle (B) so that the liquid surface (a) is lower than the low-pressure valve (A).</li> <li>Open the valve mount door.</li> <li>Use a 6 mm wrench to loosen the 5 male nuts (A) on the housing and remove the flow line tubing.</li> <li>Use an M4 Allen wrench to remove the 3 hexagon socket screws (A).</li> <li>Remove the low-pressure valve stator ASSY (A) and ring (B), taking care not to drop the ring (B). Place down with the ring side up.</li> <li>Hold the edge (a) of the low pressure valve rotor (A) and remove the rotor.</li> <li>Check for scratches on the rotating and sliding surfaces (a and b) of the low-pressure valve rotor (A) and the low-pressure valve stator (B).</li> <li>If a scratch is found through visual inspection, replace with a new low-pressure valve rotor and low-pressure valve stator.</li> <li>Disassemble the low-pressure valve stator ASSY into the low pressure valve stator (A), gasket (B), and housing (C).</li> <li>Immerse the low-pressure valve rotor (A), low-pressure valve stator (B), gasket (C), and housing (D) into a beaker filled with 2-propanol and clean for 5 minutes in an ultrasonic bath.</li> <li>Holding the edge of the low pressure valve rotor (A), align the notch (a) of the low-pressure valve rotor (A) with the alignment mark (b) of the shaft (B) and insert horizontally to fit them together.</li> <li>To assemble the stator ASSY, fit the gasket (B) and the low pressure valve stator (C) to the housing (A) in that order, then fit the ring (D).</li> <li>Face the ring side upward, hold the low-pressure valve stator ASSY (A) and the ring (B), and insert into the main unit taking care not to drop the ring.</li> <li>Attach the 3 hexagon socket screws (A) to the housing (B), and use an M4 Allen wrench to gradually tighten each screw in turn.</li> <li>Reattach the flow line tubing of each low-pressure valve port to their original positions, and use a 6 mm wrench to tighten the 5 male nuts (A).</li> <li>Close the valve mount door. Press [Next]. The next screen is displayed.</li> <li>Select the replaced part and press [Next]. The replacement history is recorded in the maintenance log.</li> <li>Check that the usage value is reset and then press [Exit].</li> </ol> <h3>Related Notes</h3><ul> <li>The [Low pressure valve rotor and stator replacement] screen can also be displayed by pressing [LPV Rotor] or [LPV Stator] on the replacement parts tab in the [Maintenance] screen.</li> <li>The low-pressure valve stator is divided into the ring and the low-pressure valve stator ASSY. Remove the ring and housing slowly because the lowpressure valve stator and gasket may drop from the lowpressure valve stator ASSY. Any scratches can cause wear and tear in a short period of time.</li> <li>As much as possible, touch only the very edge of the lowpressure valve rotor, and after fitting, use wiping paper moistened with 2-propanol to wipe the sliding surface that contacts the lowpressure valve stator.</li> <li>Tighten the 3 hexagon socket screws alternately 60° each, using the longer side of the M4 Allen wrench as a handle to firmly tighten them at the end. Do NOT tighten the hexagon socket screws unevenly.</li> </ul> <h3>Necessary Parts</h3>Low-Pressure Valve Rotor (for LC-2030) Consumable Part 228-36923-00 Qty: 1 Low-Pressure Valve Rotor (for LC-2040) Consumable Part S228-58668-01 Qty: 1 Low-Pressure Valve Stator (for LC-2030) Replacement Part 228-36917-01 Qty: 1 Low-Pressure Valve Stator (for LC-2040) Consumable Part S228-58668-01 Qty: 1 Visit our <a href="https://store.shimadzu.com/" target="_blank">webstore</a> to order parts. <h3>Necessary Tools</h3><ul> <li>6mm Wrench</li> <li>M4 Allen Wrench</li> </ul> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>

<div class="container"><h1 class="title">Replacing the Measuring Pump Plunger Seal - LC-2030</h1> <div class="center"><iframe width="800" height="450" src="https://www.youtube.com/embed/xdhO8hBMOW0?rel=0" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe></div> <h3>Procedures</h3><ol><li>Press [Plunger Seal] on the [Replacement Parts Selection] screen. The measuring pump plunger moves all the way back, and the [Measuring pump plunger seal] screen is displayed.</li> <li>Open the valve mount door.</li> <li>Use a 6 mm and an 8 mm wrench to loosen the 3 male nuts (A).</li> <li>Remove the flow line tubing from the top and bottom of the pump head and from port 1 of the low pressure valve.</li> <li>Use an M4 Allen wrench to remove the 2 hexagon socket screws (B) located on the pump head (A).</li> <li>Keep the pump head (A) level and slowly pull it away from the seal holder (B).</li> <li>Keep the seal holder (A) level and slowly pull it away from the plunger (B).</li> <li>Vertically insert the end of the seal tool (A) with a stepped side (a) into the plunger seal (B).</li> <li>Pull out the seal tool (A) vertically to remove the plunger seal (B) from the seal holder (C).</li> <li>If the inside (a) of the pump head or the fitting section (b) of the plunger seal is dirty, use wiping paper moistened with 2-propanol to wipe clean.</li> <li>Place a new plunger seal (B) on the seal holder (C).</li> <li> Vertically insert the non-stepped end (a) of the seal tool (A) into the plunger seal (B), and push down until it is level.</li> <li>Pull out the seal tool (A) vertically.</li> <li>Slowly push the seal holder (B) with the alignment mark (a) facing up onto the plunger (A).</li> <li>Then, carefully push the pump head (C) onto the seal holder horizontally.</li> <li>Attach the 2 hexagon socket screws (B) to the pump head (A), and tighten with an M4 Allen wrench.</li> <li>Reattach the tubing on the top and bottom of the pump head and the tubing to port 1 of the low-pressure valve to the original positions, and fix them using a 6 mm and 8 mm wrench.</li> <li>Close the valve mount door.</li> <li>Press [Next]. The next screen is displayed. Press [Exit].</li> <li>After finishing, perform a purge and check for liquid leakage from any gap between the pump head and the head holder or the tubing connection parts.</li> <li>The replacement history is recorded in the maintenance log.</li></ol> <h3>Necessary Parts</h3>Measuring Pump Plunger Seal Consumable Part 228-35145 Qty: 1 Visit our <a href="https://store.shimadzu.com/" target="_blank">webstore</a> to order parts. <h3>Necessary Tools</h3><ul> <li>6mm Wrench</li> <li>8mm Wrench</li> <li>M4 Allen Wrench</li> <li>Seal Tool S228-25142-01</li> </ul> <h3>Related Procedures</h3> <span class="btn-link">[[Inspecting the High-Pressure Valve Rotor and High-Pressure Valve Stator - LC-2030->Inspecting the High-Pressure Valve Rotor and the High-Pressure Valve Stator - LC-2030 (296)]]</span> <div class="buttons"> <span class="btn-back"><<back "Back">></span> </div> </div>