i-Series Plus Integrated HPLC Systems


i-Series Plus

Providing the Ultimate Balance of High-Level Performance and Ease-of-Use in an Integrated HPLC

You asked for it, you got it.


Every i-Series has a touch-screen LCD display for easy, intuitive system control and chromatogram viewing, built-in degassing, quaternary solvent delivery, autosampler and UV or PDA detector. As always, sample injections are the fastest around (< 14 seconds), with ultra-low carryover.
In response to you, our customer, the i-Series Plus expands on these features to become everything you need in an HPLC.

  • Increased Column Oven Capacity – Store up to three 300mm LC columns.
  • Optional Column Switching – Choose the analysis flow path with convenient software control.
  • Sample Pretreatment – Automate sample dilution and reagent/internal standard addition.
  • Optional Add-on Detectors – Add a refractive index or fluorescence detector.
  • Enhanced Quantitative Performance – Achieve wider linear range and repeatability for the smallest injected volumes of 1 µL or less.

Fully integrated system
Fully integrated system with all necessary HPLC components in a space saving design

The degasser, quaternary solvent delivery for gradient or isocratic elution, autosampler with Cooler Option (4 – 45 C), large capacity column oven (up to six 100 mm columns or three 300 mm columns), and detector (UV or PDA) in one compact system.


Touch-screen LCD display
Touch-screen LCD display

Easy and intuitive complete system control. Also possible for chromatogram viewing away from software.


*New to i-Series Plus* - Sample Pretreatment

Automate sample dilution and reagent/internal standard addition.

new sample pretreatment


Open Access Sample Placement
Open Access Sample Placement

A direct access mechanism enhances work efficiency by allowing users to place new samples into racks that are not involved in sample injection during analysis. And samples racks can also be shared by multiple analysts without interrupting other users’ analysis.


Enhanced Quantitative Performance

Achieve wider linear range and repeatability for the smallest injected volumes of 1 µL or less.

Enhanced quantitative performance


Possible for Add-on Detectors or Column Switching
Possible for Add-on Detectors or Column Switching

Add a refractive index or fluorescence detector.

Column switching is possible between two or six columns and is software controlled.


Hardware Specifications
Pump
Prominence-i Nexera-i
Degassing Unit Five Lines: Mobile phase 4 + Rinse solution 1 (Volume 400 µL)
Pulsation 0.1 MPa (1.0 mL/min,10 MPa, Water)
Flow rate range 0.0001 to 10 mL/min
Flow rate precision < 0.06 %RSD or < 0.02 minSD, below whichever is greater
Elution Mode Four-solvent low-pressure gradient
Gradient / range 0 to 100%, in 0.1% steps
Gradient / concentration precision ±0.1% (1 mL/min,10 MPa, Specified condition)
Maximum pressure 44 MPa (0.0001 to 5 mL/min)
22 MPa (5.0001 to 10 mL/min)
66 MPa (0.0001 to 3 mL/min)
44 MPa (3.0001 to 5 mL/min)
22 MPa (5.0001 to 10 mL/min)
Autosampler
Prominence-i Nexera-i
Injection method Needle-in-flowpath
Injection volume accuracy ±% (50 µL, N = 10)
Injection volume range 0.1 to 100 µL
(Option: 0.1 to 50 µL, 1 to 500 µL, 1 to 2,000 µL)
0.1 to 50 µL
(Option: 0.1 to 100 µL, 1 to 500 µL, 1 to 2,000 µL)
Injection volume reproducibility RSD < 0.20% (5.0−2000 µL), RSD < 0.25% (2.0−4.9 µL)
RSD < 0.5% (1.0−1.9 µL), RSD < 1.0% (0.5−0.9 µL)
Injection cycle time Minimum 14 seconds (Specified condition)
Samples capacity 336 (1 mL), 216 (1.5 mL), 112 (4 mL), 4 (MTP/DWP)
Sample cooler 4 to 45°C (Room temperature needs to be 30°C or lower and the humidity 70% or less to go down to 4°C. Room temperature needs to be 15°C or higher to go up to 45°C.)
*No sampler cooler included in LC-2030 model
Injection linearity > 0.9999 (1 to 100 µL, Specified condition) > 0.9999 (1 to 50 µL, Standard Sample Loop, Specified condition)
> 0.9999 (1 to 100 µL, 100 µL Sample Loop (Option), Specified condition)
Oven
Prominence-i Nexera-i
Oven Capacity 6 columns at 10 cm max., 3 pieces at 10 cm to 30 cm
Temperature control range Room temperature ± 12 to 90°C, Setting range 4 to 90°C
UV Detector
Prominence-i Nexera-i
Wavelength Range 190 to 700 nm
Noise level ±2.5 × 10−6 AU, (250 nm, Specified condition)
Flow cell 12 µL (10 mm, TC), 12 MPa 8 µL (10 mm, TC), 12 MPa
Option cell High-Speed: 8 µL (10 mm, TC)
Semi-micro: 2.5 µL (5mm, TC)
Conventional: 12 µL (10 mm, TC)
Semi-micro: 2.5 µL (5 mm, TC)
PDA Detector
Prominence-i Nexera-i
Wavelength Range 190 to 800 nm
Noise level ±3 × 10−6 AU, (250 nm, Specified condition)
Flow cell 10 µL (10 mm, TC), 12 MPa 8 µL (10 mm, TC), 12 MPa
Option cell High-Speed: 8 µL (10 mm, TC)
Semi-micro: 2.5 µL (5mm, TC)
Conventional: 12 µL (10 mm, TC)
Semi-micro: 2.5 µL (5 mm, TC)
Shimadzu offers the following training classes:

HPLC-101: Shimadzu Modular HPLC Training with PM
HPLC-102: Shimadzu i-Series HPLC Training with PM

Click here to learn more!

Shimadzu has launched its interactive "Virtual Advisor" websites to further enhance customer service.

>> Learn about the i-Series Virtual Advisor.

For Research Use Only. Not for use in diagnostic procedures.