CLAM-2000 Fully Automated Sample Preparation Module for LCMS


CLAM-2000-LC-MS/MS Application Examples

This analysis flow is an example of a simultaneous multicomponent analysis of steroids in serum using a fully automated preparative LCMS analysis system consisting of a Nexera X2 Ultra High Performance Liquid Chromatograph, LCMS-8060 Triple Quadrupole Mass Spectrometer, and CLAM-2000 fully automated sample preparation module for LCMS.

Pretreatment Conditions and Analysis Flow

Pretreatment Conditions and Analysis Flow

Quantitative results of drug spiked into serum

Steroids
Compounds Polarity
Range
(ng/mL)
r2 QC samples concentration (ng/mL) Accuracy %RSD (n=3)
LLOQ Midium ULOQ LLOQ Midium ULOQ LLOQ Midium ULOQ
 Aldosterone + 0.03 - 1.14 0.997 0.03 0.46 1.14 96.5 102.0 102.8 6.3 7.9 3.6
 Cortisol + 1.51 -
320
0.999 15.1 20.8 320 104.4 100.4 98.6 1.6 0.5 0.7
 DHEAS + 12.9 -
2750
0.999 12.9 179 2750 104.3 101.3 99.6 3.9 3.6 1.9
 Corticosterone + 0.29 -
62
0.998 0.29 4.03 62 101.8 101.0 98.4 4.6 0.8 10
 11-Deoxycortisol + 0.08 -
18
0.997 0.08 1.17 18 103.0 102.7 96.1 5.5 0.9 0.2
 Androstenedione + 0.08 -
18
0.999 0.08 1.17 18 116.5 99.1 100.1 4.6 1.3 1.1
 Testosterone + 0.03 -
7.2
0.999 0.03 0.47 7.2 93.8 101.4 100.1 3.7 1.0 1.0
 17-OHP + 0.12 -
26
0.998 0.12 1.69 26 103.6 103.3 99.6 6.2 1.6 1.8
 DHEA + 0.31 -
65
0.999 0.31 4.22 65 93.1 98.1 99.0 6.5 3.9 6.5
 Progesterone + 0.12 -
26.5
0.999 0.12 1.72 26.5 103.3 101.0 99.2 3.9 1.1 1.5
 Aldosterone (neg) + 0.03 -
1.14
0.996 0.03 0.46 1.14 115.0 97.3 97.4 6.6 7.5 8.0
 DHEAS (neg) + 12.9 -
444
0.997 12.9 20.8 444 101.7 103.0 99.7 2.1 1.2 7.1

For Research Use Only. Not for use in diagnostic procedures.