LITERATURE


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LC-8A Consumable Parts List
This file contains descriptions, part numbers and comments.
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LC8A Pump Provides Analytical-to-Prep Scale-Up
The LC8A Pump Provides Analytical-to-Prep Scale-Up, is versatile and easy to use, and offers single point control from system controller or CLASS-VP software.
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Total Organic Carbon Analyses for Water Purification, Injection using the 680C.
The changes to the US Pharmacopoeia monograph for the quality or purified water, water for injection, and cleaning water is under review and evaluation by the pharmaceutical industry. The current proposed method will include replacing the oxidizable substances test by measuring Total Organic Carbon (TOC) testing.
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Four-Channel Vacuum Degasser-Maximum Degassing Efficiency in an Ultra-Compact Design.
Eliminates helium degassing to save time and money/Fast, efficient degassing from up to 4 solvent channels/Steady, Self-Maintaining Vacuum/Metal-Free Bioinert Composition and Advanced Self-Diagnostics
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Increase Your Productivity with our State-of-the-Art Autosamplers
Increase Your Productivity with our State of the Art Autosamplers
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Integrating Sphere Accessory for UV1601
The Integrating Sphere Accessory for UV1601 enhances the versatility of the Shimadzu UV-1601 Spectrophotometer. In a matter of minutes, the UV-1601 can be configured for measurement of reflectance as well as diffuse transmittance.
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VOCs in Water Analysis System
The Shimadzu's GC-14AP-ENV is a turn-key gas chromatograph system for the analysis of volatile organic carbon compounds in drinking or waste water.
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PUMPS: Pulse-Free, Unsurpassed in Reliability, Minimal Maintenance, Proven Precision
Shimadzu’s LC-10AS, LC-10AT, and LC-10AD are pulse-free, unsurpassed in reliability, require minimal maintenance, have proven precision and are suitable for your most sensitive applications.
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Water Analysis Pack for UV-1201
Water Analysis Pack for UV-1201 offers fast and convenient testing for the analyst, pre-measured reagents and increased productivity with the six-cell positioner.
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Are You Reporting Valid TOC Numbers ?
Total Organic Carbon (TOC) analyzers use two oxidation processes, combustion and UV/Persulfate oxidation. <br>Reprinted from Proceedings of 1998 Water Quality Technology Conference Copyright 1998, American Water Works Association
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Determining the Correct Concentrations of TOC in Purified Water and WFI
The need for increased sensitivity in the pharmaceutical industry has lead to many stricter requirements for analyzing samples for total organic carbon (TOC)
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Continuous On-Line Monitoring of Purgeable Organic Compounds
A company with a closed wastewater system should be aware of dissolved flammable gases in the piping system. Two components present in wastewater pipes are wastewater and head space gases.
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CLASS-VP<SUP>TM</SUP> - New Tools for Better Solutions
CLASS-VP<SUP>TM</SUP> is a multi-tasking chromatography data acquisition and instrument control system operating under Microsoft Windows<SUP>TM</SUP>.
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CLASS-VP<SUP>TM</SUP> - New Tools for Better Solutions
A New Program with New Tools, to Make Chromatographic Data Processing More Powerful Than Ever acquisition and instrument control system operating under Microsoft Windows<SUP>TM</SUP>.
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TOGAS - Transformer Oil Gas Analysis System
The analysis of gas in transformer oil is not a new requirement. Care and maintenance of expensive transformers has been facilitated by routinely sampling the transformer oil and determining the concentration of various gases.
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Determining the Injection Volume and/or Range for TOC-500
There is correlation between the Range, Injection Volume, and Sample Concentration that is expressed in the following equation: (Injection Volume x Concentration) / Instrument Range = 300 (constant)
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Determination of Vitamin E in Hand Lotion
Fat soluble Vitamin E (alpha-tocopherol) is an oil found naturally in plants including wheat germ, rice and cotton seeds. In humans, Vitamin e is a potent antioxidant and is also involved in the metabolism of the trace element selenium.
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Determination of Tetrabutylammonium Ion in an Antibiotic Sample
Quaternary ammonium salts such as tetrabutylammonium bromide (TBA+Br-) are often encountered in organic chemistry. The reaction of a quaternary ammonium halide with silver oxide yields a quaternary ammonium hydroxide and a silver halide
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Automated Calibration Standard Preparation with the SIL-6B/9A
For HPLC analysis, it is often necessary to generate a calibration curve by injecting standards of several known concentrations and computing response factors based upon the linear regression of a plot peak area (or height) vs concentration.
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Separation of Tryptic Digest of Cytochrome C Using LC-7A Liquid Chromatograph
The chromatographic analysis of the tryptic digest of cytochrome C, along with many biological separations, pose special problems for standard HPLC systems. Typically mobile phases containing high salt concentrations (e.g. 1 M NaCl), or other corrosive agents such as trifluoroacetic acid, are used which will degrade the stainless steel in HPLC pumps unless the system is carefully passivated and rigorously maintained.
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Guaranteed Scale-Up From Analytical to Preparative with Shimadzu Matched Column Sets
Development of an HPLC can be a time consuming process. Development of a preparative HPLC method can be expensive as well as time consuming, when the cost of the solvents being pumped at preparative flow rates is taken into consideration. Shimadzu provides a solution to this problem by offering matched column sets with the scale-up from analytical to preparative guaranteed.
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Excellent Gradient Precision with the LPM-600 Low Pressure Quaternary Gradient Mixer
A key function of any gradient HPLC System is the ability to provide accurate and reproducible gradients. A lack of precision will result in fluctuating retention times and subsequent problems in quantitation. The Shimadzu LPM-600 Quaternary Gradient Mixer meets the requirements of precision gradients for analytical up to semi- preparative applications.
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Comparison of Semi-micro and Analytical HPLC
Development of an HPLC method can be very expensive and time consuming, especially when using the normal analytical columns and standard flowcells. Taking into account the amount of mobile phase used for these methods, the cost can increase tremendously. Shimadzu provides a solution to this problem by providing semi-micro flowcells for the SPD-10A(V), UV-Vis and RF-10A Fluorescence Detectors which can be used in conjunction with micro columns.
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Enhanced Fluorescence Sensitivity with Wavelength Programming
The Shimadzu RF-10A is a very sensitive, very flexible fluorescence detector that can be integrated with any HPLC System. Its various design features offer both high sensitivity and high selectivity. The RF-10A can perform excitation and emission wavelength scanning, and when linked via fiber optic to the SCL-10A System Controller, spectra can be viewed on the LCD display of the controller.
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Dual Wavelength Detection of a Peptide
When it comes to demanding HPLC applications, you need a reliable system providing the ultimate in flexibility and performance. The Shimadzu LC-10A Series is such a system. While every analysis is not as difficult to perform as trifluoroacetic acid (TFA)/acetonitrile peptide separations, wouldn't it be nice to have equipment which is equal to the task, reliable enough for routine applications and also a good value? Of course it would! That's why you need to look no further than Shimadzu's LC-10A.
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The Analysis of Analgesics by UV Detection
In the pharmaceutical industry, different chromatographic techniques are used in method development and drug analysis. HPLC is the most common analytical technique used in the analysis of many compounds including Analgesics, Antiarrhythmics, Antibacterials, Cardiac drugs, Cholesterol-Reducing drugs, and Vitamins. The Shimadzu LC-10A Series HPLC System provides the sensitivity, reliability and precision required for pharmaceutical analyses
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High Sensitivity Porphyrin Analysis by Fluorescence Detection using R928 PMT
The analysis and quantitation of porphyrins for diagnosis has been, until recently, very complex and time consuming. HPLC has become a very effective analytical method, since it can separate and quantitate individual porphyrins very rapidly. The Shimadzu LC-10A System with the RF-10A Fluorescence Detector offers both high sensitivity and high selectivity in the analysis of porphyrins. The sensitivity is enhanced by using the optional Photomultiplier Tube R928-08.
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LC-10AS Pump Recommended Spare Parts List (LC-014)
The following is a list of recommended spare parts for the LC-10AS Pump. This list encompasses those consumable items which are most frequently replaced.
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LC-10AD Pump Recommended Spare Parts List (LC-015)
The following is a list of recommended spare parts for the LC-10AD Pump. This list encompasses those consumable items which are most frequently replaced.
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SIL-10A Autosampler Recommended Spare Parts List (LC-016)
The following is a list of recommended spare parts for the SIL-10A Autosampler. This list encompasses those consumable items which are most frequently replaced.
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SIL-6B/9A Autosampler Recommended Spare Parts List (LC-017)
The following is a list of recommended spare parts for the SIL-6B/9A Autosampler. This list encompasses those consumable items which are most frequently replaced.
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LC-10A Series Detectors Recommended Spare Parts List (LC-018)
The following is a list of recommended spare parts for the SPD-10A/V UV/Vis Absorbance Detector and the RF-10A Fluorescence Detector. This list encompasses those consumable items which are most frequently replaced.
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HPLC Analysis of a Cold Tablet Using a 2.0 mm I.D. Column
HPLC analyses most commonly employ 4.6 mm ID columns and flow rates of 1.0 ml/min. However, the use of micro bore columns offer several advantages over the more common 4.6mm columns. The lower flow rates which are used mean that the consumption of solvent is markedly reduced.
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Performance Comparison of Variable Wavelength and Photodiode Array Detectors
Shimadzu strives to achieve superior detector performance by designing high quality optical benches. To achieve this in both the UV and Vis range, dual lamp detector design is offered for both variable wavelength and PDA models. The SPD-M10A and SPD-M10AV PDA detectors demonstrate comparable UV sensitivity. Both the SPD- M10A and M10AV are approximately one and one-half times higher in sensitivity than the SPD-M6A; and, the SPD- 10AV UV-Vis detector demonstrates six times more sensitivity than its photodiode array counterparts.
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Simultaneous Execution of 3 Separations With a Single LC-10A System
The Shimadzu LC-10A System is a versatile HPLC platform that provides ease of automation and flexibility. In response to a request from a customer performing high throughput clinical applications, we were asked to perform three separate analyses with one automated system. To accomplish this task we configured a system with two automated column selection valves, three pumps and an autosampler with cooler.
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HPLC Detection of Polynuclear Aromatic Hydrocarbons
The polynuclear aromatic hydrocarbons are a ubiquitous class of highly lipophilic, potentially carcinogenic substances consisting of a large number of individual compounds containing aromatic rings.
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Analysis of Clean In Place 150 Residues on Process Equipment Using UV Detection
The pharmaceutical industry has recently become concerned with the efficacy of cleaning procedures not only to remove active drug, but also to remove the products used to clean the process equipment. At Schein Pharmaceutical in Carmel, New York, the robotics department has developed a comprehensive program to validate the efficacy of current cleaning protocols. This program incorporates the use of a Shimadzu LC-10A Series HPLC System to detect trace amounts of cleaning agents
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Separation Of Nucleotides and Xanthines using The LC-10Ai Bioinert HPLC (LC-024)
Recently, the use of bioinert high performance liquid chromatography has increased concomitantly and most likely as a result of research burgeoning in the areas of protein and peptide chemistry. The utility of these systems stems from methodology designed to preserve biological activity and molecular conformation in biological samples.
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LC-10AT Pump Recommended Spare Parts List
The following is a list of recommended spare parts for the LC-10AT Pump. This list encompasses those consumable items which are most frequently replaced.
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LC-10Ai Pump Recommended Spare Parts List
The following is a list of recommended spare parts for the LC-10Ai Pump. This list encompasses those consumable items which are most frequently replaced.
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SIL-10AXL Autosampler Recommended Spare Parts List
The following is a list of recommended spare parts for the SIL-10AXL Autosampler. This list encompasses those consumable items which are most frequently replaced.
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SIL-10Ai Autosampler Recommended Spare Parts List
The following is a list of recommended spare parts for the SIL-10Ai Autosampler. This list encompasses those consumable items which are most frequently replaced.
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Updated Performance Comparison of Variable Wavelength and Photodiode Array Detectors
Shimadzu strives to achieve superior detector performance by designing high quality optical benches. To achieve this in both the UV and Vis range, dual lamp detector design is offered for both variable wavelength and PDA models. The purpose of this bulletin is to compare the relative performance of Shimadzu's UV-Vis and Photodiode Array Detectors.
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SIL-10AXL Autosampler Semi-Micro Kit (LC-031)
The utility and demand for semi microbore HPLC has dramatically increased for a variety of reasons: semi-microbore applications offer increased sensitivity, reduced sample consumption and a substantial reduction in liquid waste. Shimadzu now offers the option of implementing a semi-micro kit to easily and economically convert the SIL-10AXL analytical autosampler to an autosampler capable of semi microbore applications.
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Analysis of Components in Cold Medicine Using New TLC-FTIR Method
Thin layer chromatography (TLC) is an inexpensive, easy, and highly sensitive separation method used in various fields. Qualitative analysis of separated components in TLC is based on a comparison of rates of migration (Rf). There are great variations depending on the conditions so this method isn't decisive.
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Gas Chromatography Analysis of the Triglyceride Using Aluminum-Coated Capillary Column
The range of applications on many fields for fused silica columns has expanded rapidly in recent years. Fused silica columns possess an inactive internal surface, flexibility, and a chemical bonded liquid phase, all of which result in high resolution and superior performance. This application note introduces examples using this high-temperature, aluminum-coated column for the analysis of high-boiling point triglycerides.
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GC Analysis of High Boiling Point Compounds Using Aluminum-Coated Capillary Columns #2
In the past, the outer surface of fused silica capillary columns was coated with polyimide resin. These columns are now largely-coated with metallic aluminum, imparting enhanced heat resistance. The chemically bonded, thermally stable liquid phase together with its 0.1-micron thickness render these columns appropriate for good separation of high boiling point compounds. Application Note 130 describes teh features of the fused silica, aluminum-coated column, including examples of triglyceride analysis.
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Gas Chromatography Analysis of Gasoline Hydrocarbon Fractions
It is often necessary to quantify complex mixtures such as automobile gasoline into its hydrocarbon fractions. These fractions include olefinic, aromatic, and saturated hydrocarbons. There are several methods used to obtain the concentration of each fraction. Currently, there are two methods commonly used in Japan. One method is the Fluorescent Indicator Adsorption Method (FIA) as specified by Method JIS K 2536. Gas chromatography is the second method that uses the MSG C-PONA Analysis System, in which both 13X molecular sieve and polar packed TCEP packed columns are utilized.
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Gas Chromatography Analysis of Organotin Compounds by ECD
Organotin compounds have a wide range of uses, including paint for ship bottoms, fishing net anti-fouling agents, wood preservatives, fiber bacteriocide and fungicide, PVC stabilizers, and polymerization catalysts. The majority of organotin compound applications are used for ship bottom paints and fishing net anti-fouling agents. These compounds pose an environmental pollution danger to the oceans. Due to gradual dissolution of these compounds, the danger includes the risk of toxicity to marine organisms and fish after prolonged exposure. The organotin compound most used in paint for ship bottoms and fishing nets is bis-tributyltin exide (TBTTO).
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Gas Chromatography Headspace Analysis of Herbal Aroma Compounds Using GC-14A & HSS-2B
Foods are not enjoyed by taste alone. The smell or aroma contributes to simulating the appetite. Though not the major ingredient, herbs play an important role. The flavor components of herbs were analyzed using a gas chromatograph.
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Gas Chromatography Selective High Sensitivity Analysis of Tertiary Amines
Selective high sensitivity analysis at the sub-picogram level of tertiary amine compounds is now easy with the use of the surface ionization detector (SID), a new detector for gas chromatography. The simple principle behind the SID and several application analyses are introduced in this Application Note.
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Gas Chromatography Analysis of Organic Solvents Using Capillary Columns (No.1)
Organic solvents are widely used as solvents and diluents for paints, printing inks, adhesives, and composite resins. They are also widely used as degreasers for various types of products, such as electrical components. From the viewpoint of occupational safety, these organic solvents are already regulated with respect to mandatory measurement of concentrations in the work environment. In addition, proper labeling for safe identification of compounds, containing a specified concentration of an organic solvent is required.
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Gas Chromatography Analysis of Organic Solvents Using Capillary Columns #2
Of the organic substances used as solvents and diluents for paints, printing ink, adhesives and composite resins, 17 substances are required by the Japan Labor Ministry to be measured based on the work envionmental measurement standard. Recently, a part of this work was revised, adding 30 organic substances to the list of substances to be measured.
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Gas Chromatography Autoinjector (AOC-14)Improves Accuracy of Capillary Column Analysis
There are several factors that can affect analysis accuracy in gas chromatographic analysis. One of these factors, the introduction of a liquid sample, is discussed in this Application Note. When using a capillary column especially in split/splitless injection mode, separation and reproducibility can show great variation due to the method of the sample introduction.
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Gas Chromatography Analysis by ECD of Halogenated Hydrocarbons in Process/Waste Water
Ground Water plays an important role as a water resource, which may serve as drinking water after processing. Ground water contamination with halogenated hydrocarbons such as trichloroethylene has become evident in recent years. The toxicity of halogenated hydrocarbons to humans is a matter of concern. These compounds are commonly used as degreasers in factories and elsewhere, and to prevent ground water pollution from the process water and wastewater from these factories, a part of the Japan Water Pollution Prevention Regulation was revised.
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Analysis of Pesticides in Golf Course Wastewater Using Capillary Columns and Detection
GC analyses for agricultural chemicals used in golf courses have been described in Application News G143 (grass killer), G148 (monitoring of runoff water from a golf course), and G149 (monitoring of the water supply).
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Analysis of Pesticides in Golf Course Wastewater Using ECD, FTD, FPD (No 4)
The Japan Environmental Agency has published provisional permissible levels for chemicals to be used on golf courses. Results of analysis for the 21 original chemicals have been described in the Application News G148.
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Automated Trace Analysis of Nitrous Oxide N<sub>2</sub>O in Air using GC-14A with ECD
The concentration of nitrous oxide N<sub>2</sub>O in the atmosphere is reported to be 300 ppb. This can be measured using a gas chromotograph (GC) with an electron capture detector (ECD).
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Analysis of Pesticides and Herbicides Using GC-14A with Septum Purge Wide Bore Injector
The Septum Purge Wide Bore Injector offers many features including few ghost peaks, capability to operate at a low carrier gas flow rate, reduced tailing of the solvent peak.
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Analysis of Trace Impurities in Organic Solvents Using GC-17A Wide Range FID
Capillary Columns are highly effective in separation and other functions for gas chromotography, and are used in a wide variety of fields today. The GC-17A is a user-friendly instrument developed for ease of use, even with the sophisticated capabilities of capillary analyses
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Advantages of GC-17A Pressure Programming: Shorter Analysis Time/Improved Sensitivity
The advanced flow control system (AFC) of the GC-17A gas chromotograph allows the split ratio, column pressure, flow rate and other parameters to be set at the keyboard.
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Improved Reproducibility for Capillary Column Analysis Using the AOC-17 Auto Injector
Gas chromatographs are used in most laboratories. They have become indispensable as have automatic samplers for routine work in factories and other workplaces where many samples must be handled.
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Analysis of ResidualAgricultural Chemicals Using FTD, FPD and ECD (No. 4)
The Japanese Ministry of Health and Welare revised the standard analysis methods for foods, additives, and other related substances on 17 October 1992 under the auspices of the Food Sanitation Act
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Gas Chromatography Analysis of Organic Solvents Using Capillary Columns (No. 3)
Capillary Columns are used to evaluate organic solvents. Highly polar ones like the DB-WAX are normally tried first (or CBP20, an equivalent phase). If these are not satisfactory, a non-polar DB-1 phase (CBP1) can be tried.
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High Speed Gas Chromatography Analysis Using GC-17A ver 3 with Narrow Bore Column (.01mm)
The capillary column used in gas chromatography has seen amazing developments. The diameter of a column is closely related to the theoretical number of plates; the more narrow the column, the higher the obtained number of of theoretical peaks and the sharper the obtained peaks.
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Polymer Analysis Using TGA-GC/MS with CI
In mass analysis, a sample is ionized, the mass numbers are measured and qualitative analysis is performed on the basis of the obtained mass spectra. The ionization methods include electronic impact ionization (EI) and chemical ionization (CI).
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Analysis of Additives in Polymers Using Fraction Collection LC-FTIR
Infrared spectroscopy is an excellent method for identification of compounds. However, when the absorbance of components overlap, it is more difficult to identify each component. In this type of situation, it is more necessary to combine this method with a separation method such as gas chromatography (GC) or liquid chromatography (LC).
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High Sensitivity Measurement with the FTIR Spectrometer
The use of infrared spectroscopy for catalyst research began to receive much attention in the 1950s. At that time, infrared spectroscopy was not widely used, but since the first spectral measurement of CO absorbed to the surface of a metal catalyst, infrared spectroscopy has become an important tool in conducting research on the configuration of molecules absorbed to catalytic surfaces.
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Analysis of Ointment
Lotions and ointments are common household items. Ointments are used to relieve itching and inflammation, treat eczema, cure athlete's foot, and for hemorrhoid therapy. Spectrophotometric and acid-base titration methods have been conventionally used to analyze these medicines, but this requires complex sample preparation. Faster identification of active ingredients is achieved using HPLC, with only pretreatment. The analysis of the active ingredients contained in medicines for skin disorders (e.g. impetigo, eczema), analgesic treatments (e.g. arthritis, insect bites, etc.), and anti-fungal medicines (e.g. athlete's foot, ringworm, etc.) are presented in the following chromatograms. Samples were provided be Belbe Chemical Industries, Co. Ltd., Japan.
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GPC Analysis of Wetting Agent
Gel permeation chromatography (GPC), is a method widely used in surfactant analysis because it separates the components based on molecular weight. Two GPC applications are presented: a metallic soap analysis and a non-ionic surfactant agent mixture analysis.
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GPC Analysis of a Non-Ionic Surfactant Mixture
Generally, when analyzing surfactants by GPC, the length of the lipophilic group determines the separation. When surfactant mixtures are analyzed by single-column GPC, the peaks often overlap, creating identification difficulties. This Application Note describes the analysis of surfactants by combining two columns. The Shimadzu Shim-pack GPC column, packed with hydrophobic gel, was used in a series with a Shimadzu Asahipak GS hydrophilic gel column. An injection was made of a mixture of an ester (A), POE (B), and amide (C) type of non-ionic surfactants.
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Fraction Collection Chromatography Application: Handy Scaled-Up Method using LC-8A
Recently, preparative chromatography has been widely used to purify synthetic reagents and extracts, and for structural analysis. The column volumes required for preparative chromatography, however, range from 10 to 100 times those of conventional LC columns. The absolute injection amounts for preparative chromatography can range from 10(3) to 10(6) times those of conventional LC analysis. This sometimes makes preparative chromatography difficult to directly implement without changing the original analysis conditions. A simple upgrade, using the Shimadzu LC8A, circumvents this implementation difficulty.
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Herbal Medicine Analysis Application
The active ingredients of Asian herbal medicines vary with their harvest times and growing region. Their quality is determined by the amount of active ingredients they contain. These medicines may contain more than one active ingredient, which heighten their effectiveness by working synergistically. It is therefore often necessary to analyze for more than one medicine or active ingredient.
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Simultaneous UV Fluorescence Quantation of Neopterine, Uric Acid, Creatinine in Urine
Neopterine in urine makes up approximately 20-50% of the total pterine, and may be clinically related to blood and liver diseases. The neopterine concentration in urine requires creatinine correction; therefore, simultaneous determination of uric acid, creatinine, and neopterine is indispensable.
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Quantitation of Catechol Estroben in Plasma
Recently, the catechol estrogen in biological samples has received a great deal of attention due to its physiological actions. Radio-immuno Assay (RIA) is currently used as the analysis method. However, 2-hydroxyestrogen is extremely unstable and easily oxidized, and is therefore found only in small amounts. This results in poor sensitivity for analysis by the RIA method. The Shimadzu L-ECD-6A electrochemical detector, however, permits highly sensitive HPLC analysis for catechol estrogen and estrogen compounds.
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Engineering Plastics Components
Engineering plastics include polyamide, polacetal, denatured polyphenylene oxide, polysulfonyl, polycarbonate, and polyester. The recent development of HFIP (hexafluoroisopropanol) has enabled the analysis of this group of engineering plastic compounds at room temperature.
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Fraction Collection - B Carotene in Carrot Extract
b-carotene is contained in carrots, pumpkins, tomatoes, and other produce. It is an essential vitamin A precursor. An HPLC recycling valve separation technique was developed for the analysis of b-carotene from carrot extract. The effect of the recycling method was then confirmed using a photodiode array detector.
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GPC Analysis of Polymide, Polyamic Acid and 12-Nylon
The GPC analysis of polymers depends upon solvent(s) that can completely dissolve the target polymer. These solvents also serve as the mobile phase in GPC analysis. If an appropriate solvent cannot be found, analyses will not provide accurate molecular weight distribution data. With the development of GPC columns, a variety of solvents (other than the widely used THF and chloroform) can now be used. This greatly increases the type of polymers that can be analyzed by GPC. Examples of the anallysis of polyimide, polyamic acid, and nylon-12 are given below.
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Analysis of Nucleotides Using ODS Column
Analysis of nucelotides is frequently performed using an ODS column in reverse phase distribution mode. Here, application examples demonstrate the analysis of ATP in rat heart tissue and the analysis of nucleotides in tuna meat.
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Applications of Horizontal ATR
Infrared absorption analysis is widely used as a means of indentifying substances. Then measurement methods include transmission, reflection, etc., and depending on the shape of the sample, it is necessary to select the appropriate attachment for the measurement.
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Kramers-Kronig Transform Analysis and Application Examples
Reflectance is a nondestructive spectroscopic technique with many applications. However, when measuring samples which have luster, such as plastics, glass, crystals, etc., or samples exhibiting strong absorbance in the infrared region, wavelength-dependent changes in refractive index can give rise to anamalous bands in reflectance spectra.
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Micro Sampling using Infrared Microscopic Spectroscopy
Recently, dissemination of infrared microscopy has been remarkable, widening its reach into various fields. In infrared microscopic analysis, sampling of the specimen is extremely important. If only sampling can be conducted skillfully, the infrared spectral measurement will almost certainly be decisive.
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Time Course Measurement Using FTIR
When polymer materials are heated, exposed to light, or mixed, changes in their molecular structure occur over a period of time. The Fourier Transform Infrared spectrometer (FTIR) is effective in following those changes over time.
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Microanalysis Using the Microscopic ATR Mapping Technique
An FTIR-infrared microscope is indispensable for the analysis of minute areas of particles. Until now, analysis of minute areas using an FTIR-infrared microscope has almost always been conducted using spot analysis.
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TOC Determination in Sludge
To characterize and limit the amount of organic substances in waste the TOC-parameter (total organic carbon) was incorporated as determination parameter in the new TA Siedlungsabfall (TA: Technical instruction, June 1st 1993). This sum parameter which is a firm component in the water analysis already for years also becomes more and more important in the solid sample analysis. In this case, the waste depending on the content of TOC is assigned to different dump classes. In order to be classified under dump class 1 (mineral material dump) the waste may at the most include a TOC content of 1 mass percent of dry residue. For rating in dump class 2 (settlement waste dump), a maximum of 3 mass percent of dry residue is permitted.
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TOC Analysis of Drinking Water
The importance of drinking water is undisputed. That fact is underlined, too, by the great number of german laws and regulations considering this topic. Water is classified into different qualities. Besides tap water, there is natural mineral water, medical draft water, spring water and soda water. Every water shows defined characteristics and qualities and is distinguished in particular by the method of its production. For the production of drinking water, for instance, different additives are allowed. Among others, chlorine, chlorine dioxide and ozone are used for disinfecting.
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Maldi 2: Optimised sample preparation for the analysis of high molecular mass proteins
Matrix assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-ToF-MS) has, since its introduction in 19881, 2 , been used very successfully for the mass determination of molecules of various types. As a general rule in MALDI-ToF-MS, the mass resolution obtained (R = M/DM) decreases with increasing mass. This is increasingly seen with biopolymers due to adducts formed from buffer components typically present. In the case of large biopolymers (M>50 kDa) the mass resolution obtained is sometimes less than 100.
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Optimierung der Probenvorbereitung bei der Messung
Die Technik “matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry” (MALDI-TOF-MS) wird seit ihrer Einführung im Jahr 1988 zur Massenbestimmung von Molekülen unterschiedlicher Substanzklassen eingesetzt.
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Maldi 3: Optimised sample preparation for the MALDI Tof-MS Oligonucleotides
Nowadays the size of DNA fragments is determined routinely using gel electrophoresis. This method however is time consuming and inaccurate (+/-10%)1 . In large part due to extensive sequencing projects (e.g. human genome project) there is a need for an alternative method that is fast, accurate and suitable for automation.
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Optimierung der Probenvorbereitung bei der Messung von Oligonukleotiden
Die Größe von DNA Fragmenten wird heutzutage routinemäßig mit Hilfe der Gelelektrophorese bestimmt. Diese Methode ist zeitintensiv und ungenau (?10 %)1. Inbesondere aufgrund umfangreicher Sequenzierungs-projekte (z.B. Human genome project) besteht zur Zeit ein Bedarf an einer schnellen, präzisen und für einen hohen Probendurchsatz automatisierbaren Alternativmethode. Die “matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry” (MALDI-TOF-MS) wurde 1988 von Karas und Hillenkamp sowie Tanaka und Mitarbeitern eingeführt.
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High Speed Gas Chromatography Analysis For C7-C44 Hydrocarbons Using a GC-17AV3
High Speed Gas Chromatography" is a recent advance in separation science that dramatically can reduce analysis run time without sacrifice to analytical performance. This technology allows some separation times to be reduced from minutes to seconds. This Application Note examines a set of C7 to C44 hydrocarbon compounds using high speed GC.
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High Speed Gas Chromatography Analysis For Explosives Using a GC-17AV3
High Speed Gas Chromatography" is a recent advance in separation science that dramatically can reduce analysis run time without sacrifice to analytical performance. This technology allows some separation times to be reduced from minutes to seconds. This application note examines a set of explosive compounds using high speed GC.
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High Speed Gas Chromatography Analysis For Benzoid Hydrocarbons Using GC-17AV3
"High Speed Gas Chromatography" is a recent advance in separation science that dramatically can reduce analysis run time without sacrifice to analytical performance. This technology allows some separation times to be reduced from minutes to seconds. This Application Note showed results from instrumentation using the 0.1mm I.D. and 0.32mm I.D. Columns: GC-17AV3 Gas Chromatograph, AFC-17H High Pressure/High Flow Controller, SPL-17 Split/Splitless Injection Port, FID-17 Flame Ionization Detector, AOC-20I Autoinjector, and Class-VP Data Acquisition System.
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TOGAS - A Transformer Oil Gas Analysis System
The analysis of gas in transformer oil is not a new requirement. Care and maintenance of expensivetransformers has been facilitated by routinely sampling the tranformer oil and determining the concentration of various gases
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Quantification of Organic Peroxide Using FTIR and Attenuated Total Reflectance
The harmful effects of active oxygen species are well known. Peroxide formation in lubricants and fuels can damage engineand machine components. Generation of oxygen radicals in solvent presents a danger to personnal and free radical formation <i>in vivo</i>can have a multitude of deleterious physiological effects
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Assessing HPLC Peak Purity with Shimadzu Photodiode Detector for SPD-M10Avp
Traditionally, HPLC with a UV detector analysis generates chromatograms that present the analyte absorbance at a given wavelength as a function of the time. With the advent of the photo diode array detector, representation of the data in 3-dimensions is accomplished by adding the spectra if the analyte to the chromatogram
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Measurement of Lead (Pb) in Milk and Sugar
Measurement Examples using the Electro-Thermal atomization method are shown for the amount of Pb in milk and sugar.
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Real Time In-Situ Monitoring of Organic Reactions Using an FTIR Fiber Optic System
The use of the FTIR as a reaction monitor can aid in recognizing formation of intermediates as well as in determining depletion of reactants and formation of products.
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MEASUREMENT OF THE REDUCTION OF POTASSIUM
The reduction of Potassium Ferricyanide by Ascorbic Acid is a well known kinetic
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Tetrahydrocannabinol (THC) in Saliva
The National Institute on Drugs of Abuse [NIDA] and The Substance Abuse and Mental Health Services Administration [SAMSHA] determine appropriate detection levels for GCMS confirmation
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Analysis of Commercially Available Standard Materials
Introduced in this article are the results of measurements of some standard materials
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Measurement of Lead (Pb) in Milk and Sugar
Measuring examples using the electro-thermal atomization method are shown for the amount of Pb in milk and the analysis of Pb in refined white sugar, which is newly adopted from the Japanese pharmacopoeia.
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Analysis of Environmental Samples Using AA-6200
The following is an explanation of the Ca and Mg measurement results together with an explanation of some of the functions of the AA-6200.
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How to Select Graphite Tube TYpes for the Electro-thermal Atomization (Furnace Analysis Method)
Selection of graphite tubes to match the element of sample ie beneficial to the electro-thermal atomization method.
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Simultaneous Analysis of Monovalent and Divalent cations realized by new column Shim-Pack IC-C2
This is to show an example of analysis of inorganic anions, and monovalent and divalent inorganic cations in rainwater using the HIC-6A.
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Determination of Ions in Desert Sand
This is an example of analysis of desert sand sampled in Sinkiang-Uighur, China, and inorganic anions and cations were determined by the ion chromatograph HIC-6A
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Determination of Aluminum Using On-Column Derivatization
The on-column derivatization is a method of adding a derivatization reagent to a mobile phase to incude derivatization reaction in column, and isolating and detecting.
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Application of HPLC to the Analysis of Cosmetics
For the analysis of antiseptics and disinfectants contained in cosmetics, applicable methods of analysis are GC, UV-Vs spectrophotometry and HPLC.
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Analysis of Anion in Environmental Water
Shows examples of Shimadzu HPLC Analysis of environmental water by the LC-6A inorganic ion analysis system
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Analysis of Anions in Soil
For the analysis of inorganic ions, a special ion chromatograph or the reversed phase ion pair method by a HPLC is applied.
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Simultaneous Analysis of Irganox Polymer Additive using LC-MS
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Analysis of Phenoxyproprionic Type Herbicides using LC-MS
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Analysis of Bisphenol A Diglycidyl Ether Using LC-MS
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Analysis of Microcystins Using LC-MS
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Drug analysis using Co-Sense for BA LC-MS system (1)
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Analysis of fullerenes using LC-MS
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Analysis of alkyl phenyl polyoxyethylene sulfonate using LC-MS
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Analysis of benzalkonium and polyoxyethylene lauryl ether using LC-MS
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Analysis of fatty acid amidopropylbetaine using LC-MS
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Analysis of pesticides in foods using LC-MS
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Structure elucidation of trace amount of impurities in drugs using Co-Sense for LCMS system analogue
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Analysis of alkylphenols using LC-MS
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Analysis of Vitamin K in food using LC-MS
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Analysis of a drug in plasma using ON-LINE pretreatment method to remove proteins for LC-MS
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Analysis of penicillin analogue using LC-MS
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Analysis of Bisphenol A and Nonylphenol using LC-MS
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Analysis of DNPH-aldehydes using LC-MS
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Analysis of antioxidants in drug and food using LC-MS
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Application of Infrared Reflection Spectrometry -2- UV-VIS Spectrophotometry
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Determination of Phosphorus, Lead and Zinc in Water by Atomic and UV-VIS Absorption Spectrophotometry
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Determination of Fourmaldehyde by UV-Visible Spectrophotometry
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Measurement using a Reflection Measurement Attachment
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Introduction of Syringe Sipper for Solution Measurement -For Rapid Analysis of Multiple Samples-
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Example of Reflectance Measurement Using Integrating Sphere -Difference Between Diffuse Reflectance and Diffuse + Specular Reflectance Measurements-
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Quantitative Analysis using Second-order Derivative Spectrum
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Measurement of Formaldehyde Diffused from Building Materials by Absorption Spectrophotometry
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Measurement of Total Phosphorus in Water by Absorption Spectophotometry
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Measurement of Nonionic Surfactants in Water by Solid Phase Extraction-Absorption Spectroscopy
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Measurement of Hexavalent Chromium by UV-Vis Absorption Spectrophotometry -Analysis using "Water Analysis Program Pack"-
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The Introduction of SolidSpec-3700/3700DUV - High Sensitivity Measurements in Entire NIR Region
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Application of SolidSpec-3700 with the Automatic X-Y Stage -Measurement of Transmittance Distribution Map of Sample Plane-
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Measurement of Hexavalent Chromium by AbsorptionSpectrophotometry (Part 2) Analysis using UV-2450 with Convenient "Water Pack"
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Measurement of Hexavalent Chromium, Aluminum, and Nickel in Plating Solution by Absorption Spectrophotometry -Analysis of Plating Solution-
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Introduction of UV-VIS-NIR Spectrophotometer UV-3600,which Uses Three Detectors to Provide High Sensitivity in All Near-Infrared Regions.
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Introduction of Variable Angle Absolute Reflectance Attachment for the SolidSpec-3700
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Variable Angle Absolute Reflectance System for SolidSpect-3700
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Determination of Stavudine Utilizing Semi-Microbore Electrospray LC/MS
It is generally accepted that sensitivity of electrospray ionization LC/MS (ESI) isconcentration dependant, i.e., overall method sensitivity can be enhanced by scaling down the column diameter,flow rate and amount of sample injected on-column.
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Determination of Serum Extracts Plus Reserpine Spikes Utilizing SPE and ESI on QP-8000
This application note describes the determination of reserpine in serumutilizing the Solid Phase Extraction (SPE) and electrospray (ESI) in LC/MS.
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Determination of a ß-Amyloid Protein Fragment
Mutation of the transthyretin, apolipoprotein or gelsolin plasma proteins results in various forms of familial amyloidsis polyneuropathy (FAP) by the build-up of anyloid fibrils of these associated proteins and protein precursors in the human nervous system and other tissues.
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Determination of Carbamate Pesticides by APCI LC/MS
Carbamate pesticides have been used in agricultural pest control in the United States since the 1960s, as contact insecticides. Some have previously been used therapeutically as pediculocides and ectoparasiticides for humans and animals, respectively.
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On-Column Detection Limit for Testosterone by APLC LC/MS
Atmospheric pressure chemical ionization (APCI) LC/MS offers users a highly-sensitive and specific means for the determination of non-polar and semi-polar analytes, including steroids such as testosterone
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ESI Mass Spectra of Heme Proteins
The structure and function of the globular proteins including myoglobin and hemoglobin enable them to perform their functions of oxygen storage and transport and are thought to be evolved from a common ancestral oxygen-binding heme protien
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Confirmation of Aureomycin and Urea in Livestock Feed Mix by LC/MS
A livestock feed was analyzed to confirm the presence of urea and aureomycin, 7-cholrotetracycline
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Qualitative Analyses of Pharmaceuticals Utilizing a New
An important instrument capability for qualitative analysis is a collision-induced disassociation (CID). CID is achieved on the Shimadzu LCMS QP-8000 by increasing the kinetic energy of the ion beam in order to fragment intact quasi-molecular and adduct ions on the skimmer.
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Determination of the Total Organic Carbon (TOC) in Solid Samples
With growing environmental concerns in the scientific community, government, and the public, waste disposalhas become a critical topic. Before solid waste is disposed, it is necessary to determine the Total OrganicCarbon (TOC) concentration.
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Spectrophotometric Measurement of APHA Color Indices
For many years, the APHA (American Public Health Association) color scale has been used to assess the qulity of water and other materials. The scale uses reference materials consisting of a series of solutions of potassium chloroplatinate and cobalt chloride in dilute hydrochloric acid, which are assigned a standard number. This article addresses what advantages, if any, are offered by the correlation of a calculated parameter such as yellowness Index with APHA standard number as opposed to correlation of absorbance with standard number.
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Utilization of Diffuse Transmittance Measurements
Utilization of Diffuse Transmittance Measurements With the UV-2401 Spectrophotometer And Integrating Sphere Isr-220 In Establishing Quality Assurance Criteria For Translucent Materials: Application to Nearly Opaque, Colored Tape With Different Colored Strips (SSI-AP-UV-011): Spectroscopic measurements are among the most widely used in quality assurance protocols. They provide accurate, rapid and reproducible data hat can be interpreted easily and, when needed, used to facilitate corrective procedures.
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Single Beam/Double Beam Spectrophotometers - Matters, Myth and Marketing
The debate regarding the relative merits and disadvantages of single and double beam spectrophotometers continues today with unabated vigor despite the fact that, during the lifetime of spectrophotometry as an analytical tool, each design has enjoyed preeminence at various junctures.
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Shimadzu Technical Note in Applied Spectroscopy
Interferometers are nondispersive devices in which the combination of light rays results in regular patterns of constructive and destructive interference. This note consists of a brief description of certain types of interferometers, their applications, and their strength and weaknesses
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TOGAS - Tranformer Oil Gas Analysis System
The GC-14A based TOGAS System is a third generation instrument built for long term,accurate measurement of dissolved gases in mineral oil-based transformer oils
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TOGAS - Tranformer Oil Gas Analysis System
The analysis of gas in transformer oil is not a new requirement. Care and maintenace of expensive transformers has been facilitated by routinely sampling the transformer oil and determining the concentration of various gases
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TOC PC Quality Control Tracking
Growing regulatory demands have been placed on industries who must monitor either the water influent, effluent or both for levels of Total Organic Carbon (TOC). To meet these demands, analysis require a TOC analyzer capable of tracking Quality Control (QC) samples.
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Conditioning TOC Catalysts
There are several methods that may be used to condition and activate the Regular Sensitivity Catalyst. The 'regeneration' program available in the instrument software is often insufficient to thoroughly condition a new catalyst bed.
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A Method for Performing a TC Blank Check for Regular Sensitivity Catalyst
The area counts generated by injecting carbon free water into the Total Carbon (TC) combustion tube of the TOC-5000A series analyzer will give the system blank of the instrument. This feature is called the TC Blank Check
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Using Standard 40 ml VOA vials with your Shimadzu ASI-5000A
Now, your Shimadzu ASI-5000A can use standard 40 ml VOA vials for analysis of TC, IC, TOC by difference (ie TC-IC) and POC. Note: NPOC can not be performed with these vials at this time.
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Analyzing Samples As Per USP 23 Using Shimadzu TOC-5000A
The United States Pharmacopoeia has established guidelines for determining system suitability for the analysis of purified water and water for injection (USP Revision 23). This guideline also provides a means to qualify unknown samples as passing or failing.
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More Results Analyzing Samples As Per USP 23 Using Shimadzu TOC-5000A
The United States Pharmacopoeia has established guidelines for determining system suitability for the analysis of purified water and water for injection (USP Revision 23). This guideline also provides a means to qualify unknown samples as passing or failing.
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Reproducibility of the Shimadzu DSC-50
The Shimadzu DSC-50 is a heat flux differential scanning calorimeter (DSC). In the DSC-50, the sample and reference are heated and/or cooled at the same gradient rate in the same furnace with a controlled atmosphere. As the sample is heated or cooled, it will go through phase transitions. During these transitions, the sample will either absorb or radiate heat.
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Introduction To the Shimadzu -TGA-51
The Shimadzu TGA-51 Macro Thermogravimetric Analyzer is another example of Kaizen. The addition of the TGA-51 to the Shimadzu 50 series of thermal analysis modules currently in existence [DSC-50(Q), TGA-50(H), DTA-50, TMA-50(H), and TAC-50T/D] improves Shimadzu's placement and resourcefulness in the thermal analysis industry
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Macro Thermogravimetric Analysis of Calcium Oxalate
The limiting factor on most thermogravimetric analyzers (TGA) today is the amount of sample you can analyze. The new Shimadzu TGA-51 Macro Thermogravimetric Analyzer is the answer to this problem. The TGA-51 has the ability to measure weight changes on samples of up to ten grams. This is accomplished by the use of a large capacity taut-beam balance, a larger furnace, and large sample crucibles. All of this allows for increased sensitivity and sample resolution while providing a more representative sample to be analyzed.
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Macro Thermogravimetric Analysis of Epoxy Resins and Filler Content
The ability to precisely measure minute amounts of moisture and/or volatiles, by thermogravimetric analysis, can be vital to the production of a quality product. To accurately accomplish this, a large sample should be used. The Shimadzu TGA-51 allows the user to analyze a sample with a weight of up to ten grams. The larger sample size facilitates accuracy and precision in weight losses of less than one percent (>1.0%).
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Macro Thermogravimetric Analysis of Polyethylene Terepthalate Fiber
The ability to precisely measure minute amounts of moisture and/or volatiles, by thermogravimetric analysis, can be vital to the production of a quality product. To accurately accomplish this, a large sample should be used. The Shimadzu TGA-51 allows the user to analyze a sample with a weight of up to ten grams. The larger sample size facilitates accuracy and precision in weight losses of less than one percent (>1.0%)
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Macro Thermogravimetric Analysis of Film
The ability to precisely measure minute amounts of moisture and/or volatiles, by thermogravimetric analysis, can be vital to the production of a quality product. To accurately accomplish this, a large sample should be used. The Shimadzu TGA-51 allows the user to analyze a sample with a weight of up to ten grams. The larger sample size facilitates accuracy and precision in weight losses of less than one percent (>1.0%).
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Macro Thermogravimetric Analysis of Polypropylene
The ability to precisely measure minute amounts of moisture and/or volatiles, by thermogravimetric analysis, can be vital to the production of a quality product. To accurately accomplish this, a large sample should be used. The Shimadzu TGA-51 allows the user to analyze a sample with a weight of up to ten grams. The larger sample size facilitates accuracy and precision in weight losses of less than one percent (>1.0%).
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Macro Thermogravimetric Analysis of The Oxidation of Stainless Steel
The limiting factor on most thermogravimetric analyzers (TGA) today is the amount of sample you can analyze. The new Shimadzu TGA-51 Macro Thermogravimetric Analyzer is the answer to this problem. The TGA-51 has the ability to measure weight changes on samples of up to ten grams. This is accomplished by the use of a large capacity taut-beam balance, a larger furnace, and large sample crucibles. All of this allows for increased sensitivity and sample resolution while providing a more representative sample to be analyzed.
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Macro Thermogravimetric Analysis of Thin Film Coatings on Superconductors
The ability to precisely measure minute amounts of moisture and/or volatiles, by thermogravimetric analysis, can be vital to the production of a quality product. To accurately accomplish this, a large sample should be used. The Shimadzu TGA-51 allows the user to analyze a sample with a weight of up to ten grams. The larger sample size facilitates accuracy and precision in weight losses of less than one percent (>1.0%).
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Macro Thermogravimetric Analysis of Tire (SBR) Rubber
The limiting factor on most thermogravimetric analyzers (TGA) today is the amount of sample you can analyze. The new Shimadzu TGA-51 Macro Thermogravimetric Analyzer is the answer to this problem. The TGA-51 has the ability to measure weight changes on samples of up to ten grams. This is accomplished by the use of a large capacity taut-beam balance, a larger furnace, and large sample crucibles. All of this allows for increased sensitivity and sample resolution while providing a more representative sample to be analyzed.
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Standard Cleaning and Calibration Procedure for DSC-50 and DSC-50Q
This procedure is to be used for the care and maintenance of the DSC-50 and DSC-50Q. It is recommended that from time-to-time the DSC furnace be cleaned of excess material which may be deposited on the detector through normal use or from runaway samples that spill out of the cell. This procedure should be performed at one-week intervals, but can be adjusted to suit the requirements of the instrument.
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Standard Cleaning and Calibration Procedure for DTA-50
This procedure is to be used for the care and maintenance of the DTA-50 Differential Thermal Analyzer. It is recommended that from time-to-time the DTA furnace and detector be cleaned of excess material which becomes deposited on the detector through normal use or from runaway samples that spill out of the sample cell. This procedure should be performed at one-week intervals, but can be adjusted to suit the requirements of the instrument or the user.
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Standard Cleaning and Calibration Procedure for TGA-50(H) and TGA-51(H)
This procedure is to be used for the maintenance and calibration of the TGA-50(H) and TGA-51(H). Because the TGA measures the weight loss of materials by thermal separation, some of the evolved material can be deposited onto the active parts of the weighing assembly (e.g., quartz rod, hanging pan, sample cell). This can cause erroneous readings in subsequent analyses by showing excessive or exaggerated weight loss which are not indicative of the particular samples being measured.
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Standard Cleaning and Calibration Procedure for TMA-50 and TMA-50H
This procedure is to be used for the maintenance and calibration of the TMA-50 and TMA-50H. The TMA, or Thermomechanical Analyzer, is a versatile instrument which measures the coefficient of thermal expansion (CTE), penetration, or tensile strength (mainly of thin films and limp fibers) of various materials. The TMA is almost always used to measure samples well before their point of degradation. However, some materials will be degraded both purposely and accidentally, leaving deposits in and on the measuring surfaces which must be cleaned.
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TOC-5000 PC Series Quick Setup
The directions below are designed a Quick Setup procedure so that the novice user can set up an analysis in just a few minutes.
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Measurement of TC and IC in Hydrogen Peroxide (TOC-001)
The Shimadzu TOC-5000/5050 can measure TC and IC (TOC=TC-IC) in high concentrations of hydrogen peroxide without any pretreatment. Therefore, accurate measurement may be achieved without danger of contamination and loss of time due to the pretreatment method.
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High Precision Measurement of Total Carbon Dioxide in Sea Water (TOC-002)
The Green House Effect is the effect of increasing atmospheric carbon dioxide (CO2) and is an environmental phenomena being investigated globally. Investigation of the circulation of CO2 in the environment, the role of ocean absorption of CO2, and the changes in the quantity of inorganic carbons (of which CO2 is a portion) in the oceans are important keys to understanding this phenomena
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Measurement of Non-Purgeable Organic Carbon (NPOC) in Salt Water (TOC-003)
Salt water and/or seawater samples are frequently analyzed for total organic carbon/non-purgeable organic carbon (TOC/NPOC) using Total Organic Carbon analyzers. The TOC-5000 series of analyzers employs the 680C catalytically aided combustion technique. This technique minimizes the potential interferences and the corrosive effects normally associated with high salt samples.
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Measurement of CO2 in an Ethanolamine Solution (TOC-004)
In an effort to reduce the amount of CO2 discharged into the atmosphere, several technologies have been developed. One of these is to absorb the CO2 typically discharged from a fossil fuel power plant into an Ethanolamine scrubbing solution. The CO2 can be easily measured using the inorganic carbon (IC) measuring capability of the TOC-5000 series Total Organic Carbon analyzer.
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Determining the Correct Concentrations of TOC in Purified Water & Water For Injection
The need for increased sensitivity in the pharmaceutical industry has lead to many stricter requirements for analysing samples for total organic carbon (TOC). The United States Pharmacopeia (USP) is regarded as one of the foremost authorities on standards for water for injection (WFI) and Purified Water (PW). The USP method that details analysing WFI and PW for TOC is USP23.
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Determination of the Total Organic Carbon (TOC) in Solid Samples (TOC-2)
Our environmental situation, which is getting more and more critical, forces politicians as well as scientists to find possibilities for the minimization of pollutant emissions. One way for a pollutant reduction is the incineration of organic waste instead of disposing it. This possibility is the target of the German regulation “TA-Abfall” (Technical Instruction-Waste)
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TOC Determination by the Addition Method TOC=POC+NPOC (TOC-5)
Current measurement methods in TOC analysis (Total Organic Carbon) are the so-called difference method and the NPOC method (Non-Purgeable Organic Carbon)
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Determination of the TOC Content in Landfill Leachate (TOC-6)
TOC analysis is a simple method to measure the total content of inorganic and organic carbon in aqueous solutions as well as in solid samples. Since TOC analysis is a very sensitive method with detection limits of about 4 ppm, it offers the possibility to perform secondary analysis without much expenditure.
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Water Management in the manufacturing process of pharmaceutical products with TOC-5000A
Water is a raw material in the manufacturing process of pharmaceutical products and is also used for cleaning. This Application News edition features reports on the ability of the Shimadzu TOC-5000A to accomodate the requirements of GMP, JP (Japanese Pharmacopoeia) and USP (United States Pharmacopoeia) as well as comparisons with competitor TOC analyzers.
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TOC Measuring of Electroplating Solution with TOC-5000A
Organic additives are added to plating solution used in the plating process for printed circuit boards. And the additive concentration effects the drawing qualities and thermal shock of the plate.
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Drinking Water: TOC Analysis / When You Absolutely Need To Know
On Dec. 16, 1998, the U.S. Environmental Protection Agency (EPA) promulgated two drinking water regulations of interest to the environmental testing and analytical community. The goal of the first regulation, the Stage 1 Disinfection Byproducts Rule (DBPR)1, is to protect customers from disinfectants and disinfectant byproducts (DBP), while the primary goal of the second regulation, the Interim Enhanced Surface Water Treatment Rule (IESWTR)1, is to reduce microbial contamination in drinking water.
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The X-Ray Fluorescence Analysis for Paper
The X-Ray Fluorescence analysis takes an important part for analyses for copy paper. It can determine not only components of paper, but also the deposit amount of component applied to coated paper or art paper. It is also used for analyses for clay, paint, starch, etc. as an additive paper.
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The X-Ray Fluorescence Analysis for Talc
The X-Ray fluorescence analysis was carried out for talc and used for anti-friction composition, chemical, cosmetics, spinning, paper making, etc. For the qualitative analysis the powder press method was used and for the quantitative analysis the glassbeads method was used.
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The X-Ray Fluorescence Analysis for Toner
Toner consists of particles of 1 to 10 &#181m made by mixing pigment like carbon black with resin like epoxy and crushing it. The X-Ray Fluorescence analysis is the optimum method for control of inorganic components like Fe and Zn in material or products.
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Fraction Collection Options with Discovery VP
The Discovery VP preparative system is designed for purification of compounds generated by reaction processes such as combinatorial chemistry and multi-step organic synthesis, or for isolation of analytes in naturally occurring substances.
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Vitamin Analysis with an Integrated HPLC System
In the last few years, vitamin supplements have become increasingly important to consumers who are concerned about maintaining the recommended daily allowances of certain vitamins, or who are focusing on specific vitamins that show promise of disease prevention or other health benefits.
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Total Nitrogen Analysis: A New Perspective on TOC
Monitoring the nitrogen content is of great importance in many industries for many reasons. In waters and wastewaters the most interesting forms of nitrogen, in order of decreasing oxidation state, are nitrate, nitrite, ammonia, and organic nitrogen1. Organic nitrogen includes such natural materials as proteins, peptides, nucleic acids, urea, and numerous synthetic organic materials. In excessive amounts, nitrate contributes to the illness known as methemoglobinemia in infants. As a preventative measure, the current EPA-imposed maximum contaminant level (MCL) for nitrate in drinking water is 10 mg/L.
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High Throughput Analysis of Anti-Epileptic Drugs
Epilepsy is a neurological disorder characterized by varying intensity of seizures. The exact cause of epilepsy is unknown, but any kind of trauma to the brain such as head injury, high fever, large doses of drugs and other toxic substances, or interruption of blood flow to the brain can contribute to seizures.
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Steroid Analysis with an Integrated HPLC System
Steroids are a structurally unique type of drug, most commonly used for anti-inflammatory purposes. In general, they can be used to treat asthma, arthritis, allergies, and may be combined with other medications to help prevent the body from rejecting transplanted organs.
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Aromatic Acid Analysis with an Integrated HPLC System
Aromatic acids are derivatives of phenol and have a wide range of practical uses. Compounds such as benzoic acid and p-hydroxybenzoic acid are common preservatives in beverages, snack foods, and cereals. Others including salicylic acid and acetylsalicylic acid show analgesic, anti-inflammatory, and antiseptic properties.
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Sample Table Importing Using the TOC Control V Software
The TOC Control V Software is designed to adapt to the needs of our most versatile users. To increase the user-friendliness, the TOC Control V software incorporates an import function that allows the user to import a sample table directly from an ASCII *.txt file.
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Creating and Verifying an Electronic Signature Using the TOC Control V Software
Due to the requirements of the 21 CFR Part 11 rule, software systems must include features such as user access rights, audit trails, and electronic signatures. The Shimadzu TOC Control V software strives to fully comply with 21 CFR Part 11 by adopting these features. This quick guide will focus solely on the implementation of electronic signatures using the TOC Control V software.
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UV Probe Quick Setup for Photometric Module
The directions below are designed as a Quick Setup procedure for the Photometric module. This will enable the novice user to setup a quantitative analysis, such as a calibration curve, in just a few minutes.
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UV Prove Quick Setup for Kinetics Module
The directions below are designed as a Quick Setup procedure for the Kinetics module. This will enable the novice user to setup a Time Course measurement in just a few minutes.
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12 Plus One Cell Holder Increases Productivity
The new 12 plus One Cell Holder improves your productivity in a wide variety of UV-Vis applications. Designed to accept both standard and semi-micro cells, it can automatically process up to 12 samples versus the double beam reference.
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New and Improved TOC Online Sampling Method for Water and Wastewater
The TOC value is an index for the total amount of organic compounds in water and is gaining increasing popularity in process analysis.
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Introduction of the new UV-1700 UV-Vis Spectrophotometer
The UV-visible spectra are often broad and no special care is required with the resolution of the UV-VIS spectrophotometer in general. However, the spectrophotometer resolution is extremely important for the analysis of the samples with a benzene ring or samples with sharp peaks. That is, higher resolution permits more accurate qualitative analysis of peaks.
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Quantitative analysis of cis, trans, and vinyl in polybutadiene using the single-reflection ATR accessory
Application News No. A308, A304, A299, A294, and A271 previously introduced that the single-reflection ATR accessory is widely used, for qualitative analysis in particular, as it requires no pretreatment, can handle any sample shape, can analyze small sample volumes, and causes minimum damage to the prism.
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Liquid Sample Analysis with the MIRacle Single-Reflection ATR Accessory
The liquid-film method and multiple-reflection ATR (horizontal ATR) method are frequently used for liquid- sample analysis. Each method offers its own merit: the liquid-film method allows adjustment of the absorption intensity (sensitivity) with the cell thickness; and the multiple-reflection ATR method allows easy cleaning after sampling analysis.
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Measurements of thin films on a metal substrate using reflection infrared devices
The semiconductor industry has lost none of its research drive over recent years, and this research has resulted in the TVs, PCs and other familiar devices that surround us. A non-contact, non-destructive, and non-vacuum technology is required to evaluate these semiconductors
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Reflective LCD cell-gap measuring system using Multi-Spec 1500
Liquid-crystal panels (LCDS) have become increasingly important over recent years for various applications such as PCs and mobile phones. LCDs are available in various types to suit each application and the measurement of the liquid crystal thickness (cell gap) is an important topic in determining the panel characteristics.
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Introduction of ATR objective with sharp GE prism
Like the transmission method and specular reflection method, the infrared microscope-ATR method is very commonly used for the analysis of minute objects or regions. The ATR method simply requires the sample to be in close contact with the prism, eliminating pretreatment that is necessary for the transmission method. In addition, it allows analysis of wider varieties of samples than the specular reflection method.
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REPRODUCIBILITY OF THE SHIMADZU DTG-60
The Shimadzu DTG-60 is a Simultaneous Differential Thermogravimetric analyzer which combines a heat -flux type DTA with a Roberval type TGA which measure both changes in physical state of a sample, as well as weight change, as a function of temperature, over time.
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Reproducible Gradients at Ultra-Low Flow Rates
A growing number of analysts are using techniques such as microbore HPLC and LC-MS which require flow rates anywhere from 10 uL/Min to 200 uL/min. Several factors must be considered when working in this range: appropriate back pressure for reliable pump performance, efficient mixing in a very limited volume, and reduction of dead volume to minimize run time and bandspreading.
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REPRODUCIBILITY OF THE SHIMADZU DTG-60
The Shimadzu DTG-60 is a Simultaneous Differential Thermogravimetric analyzer which combines a heat -flux type DTA with a Roberval type TGA which measure both changes in physical state of a sample, as well as weight change, as a function of temperature, over time
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Reproducibility of Shimadzu 5ul Cell
A 5uL cell (SSI PN 220-92910-20; Helma P/N 105.210 QS 10mm) can be used when only very small quantities of sample are available for measurement. The objective of this test was to confirm reproducibility of the Shimadzu 5uL cell results.
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Elimination of CO2 and H2O using the FTIR 8900 and Parker FTIR Purge Gas System 75-45NA
Purging an FTIR provides benefits to both the instrument and customer. By purging the system with low moisture air, the instrument is benefited by actively preventing deterioration of the optical components of the hardware, especially the beamsplitter, which is usually hygroscopic in nature. The customer is also benefited when this is matched with low CO2 containing purge air by eliminating interferences from the large CO2 band and water vapor bands.
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Analog and Photon Counting Detection in Fluorescence Measurements
Fluorescence spectroscopy is widely used in the physical and life sciences due to its selectivity and sensitivity. A number of detectors are used in fluorescence measurements including photomultipliers (PMTs), diodes, and CCDs. In many commercially available fluorescence instruments, photomultipliers are used as detectors as a result of their favorable response times, wavelength sensitivity, radiant sensitivity, and wide dynamic range.
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SIL-HTc COMPETITIVE CARRYOVER PERFORMANCE ANALYZED BY LC-MS/MS: a Collaborative Study between Shimadzu Marketing Center and Covance Laboratories
Advances in the field of drug discovery have resulted in a tremendous production of potentially therapeutic compounds and, thus, another “bottleneck” downstream from synthesis to drug development.
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Comparison of three TOC methods on a single analyzer
In the past, persulfate-based total organic carbon (TOC) analyzers were noted for their ability to achieve highly sensitive TOC analysis. However, with the advantages came disadvantages
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The HPLC Analysis of Underivatized Amino Acids
Because they lack suitable chromophores, the traditional approach to analyzing amino acids by HPLC is either pre- or post-column derivatization and detection by fluorescence. While this approach yields excellent sensitivity, the derivatization process is time consuming and may introduce variability from run to run.
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The HPLC Analysis of Carbohydrates
Carbohydrates from simple sugars to oligo- and poly-saccharides represent an HPLC detection challenge in that they are non-chromophoric. Traditional approaches to their detection, RI and UV (195 - 205nm), are problematic due to issues of poor sensitivity, long detector equilibration times and an inability to handle gradient elution.
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The Shimadzu ELSD-LT Evaporative Light Scattering Detector
Evaporative Light Scattering (ELS) detection has entered the HPLC detection mainstream in recent years. It is a valuable complement to UV, PDA and MS, but it is also a preferred detector for such applications as carbohydrates, phospholipids, triglycerides and surfactants. The ELSD makes a light scattering measurement of target analytes that have been dried of mobile phase through evaporation.
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The HPLC Analysis of Black Cohosh Using an INA Method
The Institute for Nutraceutical Advancement (INA) is a consortium of research and commercial laboratories whose mission is the development of standardized methods for the analysis of natural products. A myriad of nutraceutical products is available to health-conscious consumers.
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New Breakthrough in Wet Chemical TOC Analysis
The goal of this discussion is to demonstrate that the Shimadzu TOC-VW can effectively oxidize the Humic Acid (HA) matrix at high accuracy and precision levels never before witnessed by the wet chemical TOC community. The Shimadzu TOC-VW Carbon analyzer is the only TOC on the market that uses three oxidation techniques of UV light, Heat, and persulfate in a single analyzer
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New Breakthrough in Wet Chemical TOC Analysis
The goal of this discussion is to demonstrate that the Shimadzu TOC-VW can effectively oxidize the Humic Acid (HA) matrix at high accuracy and precision levels never before witnessed by the wet chemical TOC community. The Shimadzu TOC-VW Carbon analyzer is the only TOC on the market that uses three oxidation techniques of UV light, Heat, and persulfate in a single analyzer.
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A Modified INA Method for the Analysis of Milk Thistle Using
One of the widely available nutraceutical products seen in supermarkets is a powdered form of Milk Thistle or silybum marianum, derived from the plant native to Mediterranean Europe. It has been used from ancient times as a liver tonic. The milky sap produced by Milk Thistle contains flavolignans, collectively known as the silymarin group. Growing consumer interest, combined with the low cost and growing availability of herbal supplements and remedies, has driven the need for standardization of these products.
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High Accuracy Measurement of TOC below 5ppb Levels
The current regulatory demands of the industry require analytical instruments that can measure at increasingly lower detection limits with high precision and accuracy. For example, some ultrapure applications in TOC require trace level detection to ensure highly accurate and precise results.
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Automatic Export using TOC Control V Software Version 1.05.01 and Greater
The automatic ASCII export can be enabled in [the Administration] window of the software.
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Sample Table Importing Using the TOC Control V Software
The TOC Control V Software is designed to adapt to the needs of our most versatile users.
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Quality Control Tracking using the TOC Control V Software
Due to increased regulatory demands for high quality data, industries must have in place a stringent Quality Assurance/Quality Control (QA/QC) program. Industries and Laboratories, who monitor water influent, effluent, or both for levels of Total Organic Carbon (TOC), must be able to validate the TOC data results with Quality Control (QC) samples.
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TNb determination in the monitoring and control of municipal wastewater treatment plants
Regulations regarding continuous improvements in water protection, which accompany the changing needs of a growing metropolis, greatly increase the responsibilities and demands of municipal wastewater treatment plant operators.
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Around the clock operation: The TOC-4100 in wastewater treatment plants
The central wastewater treatment plant in Ans-bach, Germany, processes, since the start up operation in 1980, the wastewater of a city of 40.000 people and all local industrial wastewater, particularly from the meat processing companies in the area.
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Humic Acid- Complex Problem, Simple Solution
The goal of this article is to demonstrate that the Shimadzu TOC-VW can effectively oxidize the Humic Acid (HA) matrix.
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DNA Detection Limits
Shimadzu UV-Visible instruments can be used effectively for applications within the biotechnology field. The Bio-Mini-1240 and BioSpec-1601 specifically are well suited to these applications because of their built-in software features.
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Evaporative Light Scattering Detection as a Complement to UV for Flavor Profiling Peppers by HPLC
This data supports the use of 280nm UV for determining only the capsaicins as it is highly selective for those constituents. The ELSD-LT offers numerous advantages: better baseline response for the gradient method, and detection of all flavor constituents, both chromophoric and non-chromophoric, in one run.
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ELSD and UV - Complementary Detectors
Reversed phase HPLC with UV detection at 210nm has been used to determine constituents in commercial Stevia products, but the methodology is limited in its ability to separate all components of interest, especially when higher order oligosaccharides are present.
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Measuring TOC in Electroplating Solution through Wet Chemical Oxidation
Organic additives are used in electroplating solutions; these additives are used in a variety of electroplating applications form circuit board production to jewelry manufacturing. The concentrations of these additives affect the overall performance of the plating; so in many cases it is important to accurately monitor the addition of these additives.
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5310 Total Organic Carbon and Waste Water
The organic carbon in water and wastewater is composed of a number of organic compounds in various oxidation states.
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Measuring Samples Per USP 26 Using Shimadzu TOC-VC*H
Shimadzu’s TOC-VC*H analyzer is perfectly adapted to meet the requirements of USP Rev. 26 Method <643>. The TOC-VC*H analyzer utilizes 680°C heat and platinum catalyst to provide a lower maintenance instrument with a detection limit of 0.0040 mg per liter.
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Shimadzu’s Unique TOC/TN Sampling System Improves
Shimadzu’s TOC-VC 8-port valve sampling system was developed with the needs of the user in mind. This unique system saves laboratory time and work while increasing productivity and efficiency. This is the most capable and functional sampling system ever developed
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Determining the Correct Concentrations of TOC in Purified Water and WFI
The need for increased sensitivity in the pharmaceutical industry has lead to many stricter requirements for analyzing samples for TOC.
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The HPLC Determination of Salicin and Polyphenolic Glucosides
Extract from the bark of the White or European Willow, salix alba, has been used as folk medicine for many years in treating inflammation, pain and fever. The principle component of the water extract from salix bark is salicin, 2-(hydroxymethyl)phenyl-â-D-glucopyranoside, a human-metabolic precursor to salicylic acid.
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High-speed tensile testing is possible with Autograph
In response to requests for faster testing, we've developed a high-speed model. 6000 mm/min high-speed tensile testing is possible. (1000 mm/min with the standard model.)
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With Autograph, cycle tests can be run for 100,000 cycles!
Do you sense that product reliability is a bigger issue than ever? Considering product application environments, durability needs to be evaluated to about 100,000 cycles.
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Reading data from multiple channels with Autograph
The common method for evaluating the strength of automotive or other parts is applying strain gauges on parts to determine which areas will deform, collecting the strain data using
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True Strain Control testing on Autograph!
Tensile tests are often conducted with constant tensile rate applied to the specimen. However, tensile
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Peeling Tests on Heated Adhesive Tape
In the semiconductor industry, with the increase in the demand, improvements in production efficiency and the quality of products are becoming more and more important.
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Hold specimens with no load before testing (to protect specimen)
The system where TRAPEZIUM2 is used with the Autograph AG-IS or EZGraph offers the convenience
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Autograph Measures Static Friction Coefficients and Kinetic Friction Coefficients
The Autograph performs tensile, compression, and bending tests on specimens to evaluate their characteristics. By attaching special test jigs, the Autograph can also determine the coefficients of static and kinetic friction between plastic films, and between specimens made of different materials such as plastics, wood, and metals.
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EFFECTS OF INSTRUMENT RESOLUTION ON ASSESSMENT OF DNA PURITY
The traditional method for quantification and assessment of purity of DNA samples is spectrophotometric measurement at 260 and 280nm (1). It is generally accepted that a sample of pure, double-stranded DNA of 50 µg/mL will have an absorbance at 260nm of 1.0 and that the ratio of absorbance at 260 and 280nm of this sample will be greater than 1.8 (2)
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The Determination of Drug Composition and Manufacturer through FTIR Analysis
FTIR has been used for many years to identify or confirm the presence of many drugs. Through the use of spectral databases, FTIR provides a relatively easy way to determine an unknown drug
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ATOMIC ABSORPTION SPECTROPHOTOMETRY DATA SHEET No.9
Calibration Curves of Standard Solutions Using AA-6200
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Determination of Metal Elements in Montmorillonite by AAS
Various ceramic materials are widely used in many fields and include oxides, silicates and their minerals as well as clay minerals.
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Measurement of Trace Elements by Graphite Furnace AAS
Concerning measurement of components in iron and steel, 17 components are specified in the Japanese Industrial Standard (JIS) in section G1257
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Measurement of Metal Elements Using the HVG-1
Environmental destruction and environmental protection are major topics in newspaprts and other mass media and the need to measure extreme traces of harmful metals promptly and precisely is necessary.
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Determination of Phosphorus, Lead and Zinc in Water by Atomic and UV-VIS Absorption Spectrometry.
As the number of substances that pollute our water grows, the problems of establishing and maintaining water quality become complex.
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Analysis of Aluminum Alloy by Computer Controlled AA-660
AA spectrometry is being applied in various fields as an essential for the determination of metal elements.
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Atomic Absorption Spectrophotometry of Plants Using a Hydrochloric Acid Extraction Method
AA spectrophotometers, which are standard instruments for metal, are widely applied in the measurement of plant samples.
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Determination of Elemental Metals Using the HVG-1
This is to show examples of measurement of Hg, Sb and Bi in wastewater using the BHG-1 hydride vapor generator.
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Composition analysis of thin film by atomic absorption analysis
A thin film of indium, antimony and tellurium was formed on a quartz glass plate, in ultrahigh vacuum.
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Determination of Metal Elements in Organic Solvent by Electrothermal AA Spectrometry
Introduced in this article are examples of determination of lead and arsenic in naphtha and copper in oil.
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Analysis of Commercially Available Standard Materials by AA-6700
Introduced in this article are the results of measurements of some standard materials and the methods with the sample pretreatment.
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Analysis of Berberine in Crude Drugs
The SPD-M6A, employing a photodiode array sensor, is capable of storing data in the wavelength range of 195 nm to 870 nm, while observing the chromatogram at two wavelengths selected arbitraily in real time.
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Analysis of Ointment
By using HPLC, active ingredients in products can be identified and determined in a short time, only by a very simple pretreatment.
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Applications of HPLC to Pharmacognosy
Active ingredients in crude drugs vary in contents depending on the sampling time and place of origin, and the qualty evaluation of the crude drugs is determined by the contents of the ingredients.
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Applications in Food Analysis (16)
The fungicide methods shown here, to protect plants and fruit, were adopted from the reversed phase ion-pair chromatographic technique for these substances
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Application of Preparative Liquid Chromatography (3)
In order to successfully conduct a recycle separation, it is necessary to reduce extra-column band broadening.
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MEASURING POC AND NPOC USING TOC-V CSH
The chemical and physical properties of organic solvents affect their solubility in water.
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Analysis of Polycyclic Aromatic Hydrocarbone with a Multi-Functional Fluorescence Detector
The multi-functional fluorescence detector RF-550, capable of setting or varying the excitation and fluorescence wavelength by the time program is a new tool useful in simultaneous analysis of plural components differing in the detection optimum wavelength.
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High Sensitivity and High Selectivity Analysis of Ammonia with HPLC.
Quantitative measurement of ammonia is being performed in a wide field, including quality control, measurement in working environment, monitoring of environmental atmosphere, as well as in the field of biochemistry.
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Analysis of Hair Tonics
Shown in this application note is a chromatogram of Hinokitiol standard, and one in hair tonic. In this analysis, the hair tonic was diluted with methanol and a sample of it was injected into the HPLC for analysis.
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Matrix Modifier for Furnace AA
Matrix Modifiers are also known as interference suppressants or chemical modifiers. Matrix modifier is a generic term for a substance used to achieve various effects, such as increased sensitivity or repeatability.
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Stability of Flame Measurements with an autosampler
This Application News introduces the flame measurements of the elements Cd, Cu, Fe, Mn and Pb over a long period, using a standard titanium burner head.
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Estimation of uncertainty in flame atomic absorption spectrophotometry
This Application News presents a sample of uncertainty calculation for flame atomic absorption spectrophotometry on calcium in mineral water.
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Analysis of Citrus Leaves by AA-6800 NaF
The following is an example of flame analysis for a standard material using the AA-6800. The standard material used for analysis was NIST-1572 Citrus Leaves. The analysis element was sodium.
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Analysis of Rice Flour with AA-6800
The following is an example of flame analysis for standard material using the AA-6800. The standard material used for analysis was NIST-1568 Rice Flour. The analysis element was arsenic.
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Analysis of Pond Sediment Using AA-6800
The following is an example of flame analysis for standard material using the AA-6800. The standard material used for analysis was NIES-2 Pond Sediment. The analysis element was arsenic.
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Analysis of Standard Substances Using AA-6800
The following is an example of flame analysis for standard material using the AA-6800. The standard material used for analysis was NIST-1643D Trace Elements in Water. The analysis element was arsenic.
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Analysis of Citrus Leaves by AA-6800 HVG
The following is an example of flame analysis for standard material using the AA-6800. The standard material used for analysis was NIST-1572 Citrus Leaves. The analysis element was arsenic.
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Analysis of Citrus Leaves by AA-6800 MGF
The following is an example of flame analysis for standard material using the AA-6800. The standard material used for analysis was NIST-1572 citrus leaves. And, the analysis element was magnesium.
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Analysis of Standard Substances Using AA-6800
The following is an example of flame analysis for a standard material using the AA-6800. The standard material used for analysis was NIST-1572 Citrus Leaves. And the analysis element was potassium.
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Analysis of Citrus Leaves by AA-6800 CAF
The following is an example of flame analysis for standard material using the AA-6800. The standard material used for analysis was NIST-1572 Citrus Leaves. And, the analysis element was calcium.
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Analysis of Standard Substances Using AA-6800
The following is an example of flame analysis for standard material using the AA-6800. The standard material used for analysis was NIST-1643d trace elements in water. And, the analysis element was strontium.
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Analysis of Standard Substances Using AA-6800
The following is an example of flame analysis for standard material using the AA-6800. The standard material used for analysis was NIST-1643d trace elements in water. And, the analysis element was lithium.
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TYPES OF TOC DETECTORS
Total Organic Carbon (TOC), can be detected by using either the Non-Dispersive Infrared (NDIR) or the conductivity detector. The NDIR is more popular than the conductivity detector, due to stability and interference issues. NDIR detectors measure carbon, or more specifically CO2 in the gas phase, while conductivity detectors measure CO2 in the liquid or aqueous phase.
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EFFECTS OF INSTRUMENT RESOLUTION ON ASSESSMENT OF DNA PURITY
The traditional method for quantification and assessment of purity of DNA samples is spectrophotometric measurement at 260 and 280nm.
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Eppendorf UVette with Adapter UVette
The purpose of this application is to test the effectiveness of the Eppendorf® UVette on several Shimadzu spectrophotometers.
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Improving TOC Productivity: Four Solutions to over range samples
TOC measurements are becoming more and more assimilated into today's manufacturing protocols and processes. Whether it is qualifying raw material, quality assuring of a product or monitoring effluent for discharge; stopping production can be expensive. For this reason the TOC-V series of instruments offers four solutions to over range samples; automatic selection of best of three calibration curves, automatic reduction of injection volume, automatic dilution of over range sample, and automatic concentration correction for bench top dilutions.
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A guide for the TOC-Vc to low level analysis
A 2 page guide for the TOC-Vc to low level analysis. The guide covers your environment conditions, preparing standards and the preferred method of analysis.
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A guide for the TOC-Vw to low level analysis
A 2 page guide for the TOC-Vw to low level analysis. The guide covers your environment conditions, preparing standards and the preferred method of analysis.
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Analysis of Amoxicillin using the LCMS-2010EV and the LCMS-IT-TOF
<p>Amoxicillin is a drug belonging to a class of compounds known as b-lactam antibiotics.&nbsp; Amoxicillin, a member of the penicillin family, is used most often to treat a number of bacterial infections including H. influenzae, N. gonorrhoea, E. coli, Pneumococci, Streptococci, and some strains of Staphylococci.&nbsp; It is thought that these penicillin-derived compounds work to stop the bacteria from multiplying by inhibiting its cell wall synthesis.&nbsp; </p>
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Using the Shimadzu GC System in the Fuel-Grade Ethanol Production Laboratory
In the case of the ethanol production plant, GC analysis is used to determine the total amount of denaturant added to the final ethanol product prior to shipment. This analysis is required in certain ethanol markets. The denaturant, being a petroleum distillate product, can contain hundreds of different compounds. The GC analysis for ASTM D5501 certification measures the amount of methanol, ethanol, and total amount of denaturant as area percents. These area percents are corrected, per the ASTM method, to give corrected volume percentages for these materials.
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Using the Shimadzu HPLC System in the Fuel-Grade Ethanol Production Laboratory
Liquid Chromatography (LC) is any chromatographic procedure in which the moving phase is a liquid. In most cases, a stationary phase is also necessary. High Performance Liquid Chromatography (HPLC) has become the most widely applicable method of chromatographic analysis. Through use of highly efficient column materials (the stationary phase), pumping systems that provide a consistent flow of the mobile phase, and detection components designed to accommodate the needs of the analysis, HPLC is now a commonly used technique for monitoring the progress of the fermentation in the ethanol production laboratory.
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Structural Analysis of Glycopeptides (RNase B) Utilizing Accurate MSn Measurement
Glycoproteins are an essential component of cell membranes, and elucidation of their functions in relation to oncogenesis and/or viral infections is receiving attention in the search for cures, treatments, and vaccines. As glycoprotein diversity plays a key role in intercellular recognition, determination of sugar chain structure, and the sequence of amino acids bonded to sugar chains, is required for functional prediction. The LCMS-IT-TOF is ideal for sugar chain analysis as it is capable of producing high mass accuracy MSn spectra; this ability provides the power to elucidate the often complex sequences of these compounds. Moreover, analysis of small quantities of a biological sample is possible when combining the LCMS-IT-TOF with a nanoESI interface.
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Fast LC Analysis of Soft Drinks
This application note details the separation and detection of five beverage compounds with fast LC gradient conditions.
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Analysis of Polyphenols in Green Tea using the LCMS-IT-TOF
The polyphenols or catechins in green tea are known to possess many health benefits for individuals. These species possess antioxidative activity and are also thought to have chemopreventive effects against certain cancers. The antioxidative activity of catechins is theorized to help improve hypertension, reduce inflammation, and improve cognitive dysfunction in the elderly all of which are beneficial to one’s health. The analysis of this class of compounds will continue to gain in importance as more individuals and companies (particularly those in the nutraceutical industry) discuss their potential health benefits.
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GC Analysis of Biodiesel for Free and Total Glycerines by ASTM-6584
Rising prices of diesel fuel are creating a market for alternative sources. One of those sources is diesel fuel derived from Vegetable Oil.Diesel fuel made from plants has the potential to contain glycerols in the form of free glycerin, mono, di and trigl
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High-Speed, High-Resolution Analytical Applications (Part 9) Simultaneous Determination of Tryptophan Metabolites
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Analysis of Biofuel (Part 2) Determination of Formic Acid, Acetic Acid, and Propionic Acid in Biodiesel - Diesel Fuel Blend
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Application of High-Speed, High-Resolution Analysis (Part 5) Analysis of Artificial Colorings
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Application of High-Speed, High-Resolution Analysis (Part 4) Analysis of Cephem Antibiotics
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Application of High-Speed, High-Resolution Analysis (Part 3) Analysis of Isothiocyanates and Sinignin
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Analysis of Biofuel (Part 1) Determination of Fatty Acid Methyl Esters and Triglycerides in Biodiesel Blended Fuel
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Analysis of Natamycin in Cheese
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Analysis of α-Lipoic Acid in Dietary Supplement
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Analysis of N-Methylcarbamate Pesticides in Foods
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High Speed, High Resolution Analytical Applications (Part 2) Analysis of Isoflavones in Soy
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High Speed, High Resolution Analytical Applications (Part 1)Introducing Newly-Developed “Shim-pack XR-ODS” Column
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Determination of Cyanidin-3-Glucoside in Black Soybeans
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Determination of Polyether Antibiotics in Animal Feeds (Part 2) Analysis using Fluorometric Detection
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Determination of Polyether Antibiotics in Animal Feeds (Part 1) Analysis by Post-column VIS Photometric Derivatization Method
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Determination of Quinoxaline-2-Carboxylic Acid in Pork
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GPC Cleanup Methods in Pesticides Residue Analysis of Food(Part2)
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Analysis of Capsaicinoids in Spices
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Analysis of Hexavalent Chromium by Post-column Derivatization Method
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Analysis of Impurities in Drugs by Prominence Series UV-VIS Detectors (Part 2)
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Analysis of Sudan Dyes in Foods
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Analysis of Canthaxanthin and Astaxanthin
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Analysis of Malachite Green in Cultured Fish (Part 2) Simultaneous Analysis with Leucomalachite Green
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Determination of Coenzyme Q10 in Food
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Determination of Drugs in Blood Plasma by “Co-Sense for BA” (Part 7)
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Analysis of Impurities in Drugs by Prominence Series UV-VIS Detectors
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Analysis of Bromate by Newly Developed Shim-pack IC-Bromate Column
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Analysis of Malachite Green in Cultured Fish
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Analysis of Amino Acids in Vinegar
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Analysis of Hyaluronic Acid
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Analysis of Lycopene and β-Carotene in Tomato
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Determination of Benzoyl Peroxide in Foods
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Analysis of Tricyclic Antidepressants
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Determination of Drugs in Blood Plasma by “Co-Sense for BA” (Part 6)
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Analysis of Enrofloxacin in Broiled Eels
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Analysis of Glyphosate in Drinking Water by HPLC
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Analysis of Iprodione, Asulam, Thiophanate-methyl, and Siduron in Drinking Water by HPLC
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Analysis of Diquat in Drinking Water by HPLC
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Analysis of ClO2 -, ClO3 -, and ClO2 in Tap Water by Ion Chromatography
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Analysis of NO2-N in Drinking Water by Ion Chromatography
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Direct Injection of Blood Plasma for the Determination of Drugs using “Co-Sense for BA” (Part 5)
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Analysis of Chlorogenic Acid in Coffee
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Direct Injection of Blood Plasma for the Determination of Drugs using “Co-Sense for BA” (Part 4)
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Determination of Catechins in Tea
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Analysis of Anionic Surfactants by HPLC in Accordance with the Revised Water Quality Testing Method
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Analysis of Bromate by Post-column Ion Chromatography
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Analysis of Cyanide Ion and Cyanogen Chloride by Post-column Ion Chromatography
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Determination of Alliin in Garlic
View here (23 KB)

Determination of Theanine in Tea
View here (41 KB)

Application of Evaporative Light Scattering Detector ELSD-LT (Part 3) Analysis of Surfactants
View here (34 KB)

Determination of Sucralose in Soft Drinks
View here (32 KB)

Analysis of Pesticides Residues by HPLC
View here (37 KB)

Analysis of Voglibose by Post-column Fluorometric Detection Method
View here (26 KB)

Application of Evaporative Light Scattering Detector ELSD-LT (Part 2) Analysis of Oligosaccharides in Beer
View here (71 KB)

Direct Injection of Blood Plasma for the Determination of Drugs using “Co-Sense for BA” (Part3)
View here (35 KB)

Analysis of Amino Acids in Fermented Food and Drinks
View here (42 KB)

Application of Evaporative Light-Scattering Detector “ELSD-LT”(Part1) Analysis of Triglycerides in Cooking Oil
View here (39 KB)

Determination of Water-Soluble Vitamins in “Foods with Nutrient Function Claims”
View here (43 KB)

Determination of Hydrocarbon Types in Diesel Fuel According to the JPI* Method *(Japan Petroleum Institute)
View here (53 KB)

Direct Injection of Blood Plasma for the Determination of Drugs using “Co-Sense for BA” (Part 2)
View here (52 KB)

Direct Injection of Blood Plasma for the Determination of Drugs using “Co-Sense for BA” (Part 1)
View here (62 KB)

HPLC Analysis of Isocyanuric Acid and LAS Anionic Surfactant by the JWWA* Method (* Japan Water Works Association)
View here (53 KB)

HPLC Analysis of Carbofuran, Methomyl, and Carbaryl by the JWWA* Method (* Japan Water Works Association)
View here (28 KB)

Analysis of Phenolic Antioxidants in Food
View here (36 KB)

Rapid and Sensitive Analysis of Aluminum Ions using On-column Derivatization (Part 2) - Application to the Analysis of luminum Ions in Total Parenteral Nutrition Solutions
View here (73 KB)

Rapid and Sensitive Analysis of Aluminum Ions using On-column Derivatization (Part 1)
View here (22 KB)

Analysis of Polyether Antibiotics in Animal Feed
View here (24 KB)

Analysis of Potassium Bromate in Bread
View here (20 KB)

Analysis of Bromate Ions in Drinking Water
View here (45 KB)

Analysis of Animal Drug (2)
View here (35 KB)

Shim-pack HT-ODS ・New High Throughput HPLC Column
View here (23 KB)

Analysis of Sulfurous Acid Ion
View here (23 KB)

Study of Analytical Conditions for Aldehydes in Ambient Air
View here (27 KB)

Analysis of Bisphenol A using High-Performance Liquid Chromatography
View here (29 KB)

Determination of Fumonisins in Corn
View here (25 KB)

Determination of Formaldehyde and Acetaldehyde in Environmental Air
View here (78 KB)

New Standard ODS Column “Shim-pack VP-ODS” Supports
View here (50 KB)

Determination of Microcystins in Blue-green Algae in Lake Water
View here (107 KB)

Analysis of Chlorophyll (a) in Seawater
View here (141 KB)

Analysis of Proteins and Peptides using LC-MS
View here (217 KB)

Analysis of Sudan Dyes and Para Red using LC-MS
View here (73 KB)

Analysis Using Dual Ion Source DUIS-2010 (Part 2)
View here (92 KB)

Accurate Mass Analysis of Flavonoids by LCMS-IT-TOF
View here (111 KB)

Analysis Using Dual Ion Source DUIS-2010(Part1)
View here (61 KB)

Analysis of Steroidal Anti-Inflammatory Drugs Using LC/MS
View here (64 KB)

Drug Analysis Using “Co-Sense for BA” LC/MS System (2)
View here (62 KB)

LC-MSAnalysis of Compounds Related to Nucleic Acids
View here (64 KB)

Simultaneous Analysis of Pesticides in Environment Water using LC-MS
View here (166 KB)

Simultaneous Analysis of Pesticides using LC-MS
View here (78 KB)

Analysis of Catechins Using LCMS-2010EV
View here (53 KB)

Simultaneous Detection by MS, PDA and Fluorescence Detectors with LCMS-2010A System
View here (126 KB)

Simultaneous Analysis of Carbamate Pesticides with LC-MS
View here (183 KB)

Analysis of Aminoglycoside Antibiotics(Gentamicins & Neomycin)(1)
View here (55 KB)

Analysis of N-nitroso-fenfluramine using LC-MS
View here (51 KB)

Analysis of aflatoxins using LCMS-2010A
View here (157 KB)

Analysis of Microcystins using LS-MS (No. 2)
View here (176 KB)

Measurement of Enzyme Activity with the UV-3101PC Kinetics Software Package
View here (146 KB)

Reflective LCD cell-gap measuring system using MultiSpec-1500
View here (189 KB)

Analysis of Vegetable Oils using Gas Chromatography
View here (2370 KB)

Application of CBP Wide Bore Capillary Column (0.53mm I.D.)(2) -Analysis by Total Amount Injection-
View here (164 KB)

Analysis of Glycols and Monoalkylether Glycols with FS Columns
View here (80 KB)

Analysis of Organic Solvent by Headspace Analysis System
View here (96 KB)

Analysis of Halogenated Hydrocarbons in Industrial Water and Waste Water
View here (173 KB)

Determination of Four Offensive Odor Components
View here (100 KB)

Determination of Halogenated Hydrocarbons in Industrial Feed Water and Waste Water by Capillary Column GC
View here (115 KB)

Application of Antihistamics and Tranquilizers in Body Fluids by a Selective Detector (ECD,FTD)
View here (370 KB)

Analysis of SOA in Gasoline by Gas Chromatograph -Discrimination of Hydrocarbons in Gasoline-
View here (170 KB)

Analysis of Scattered Herbicides on Golf Course
View here (146 KB)

Headspace Gas Chromatography for Ethyl Caproate and Ethyl Caprylate in Alcoholic Beverages
View here (94 KB)

Gas Chromatographic Separation of Halogenated Hydro Carbons by Various Wide Bore Capillary Columns
View here (91 KB)

Analysis of High Boiling Point Compounds by Programmed Temperature Vaporizer (PTV) Injection System
View here (102 KB)

Analysis of Pesticides on Golf Course with Packed Column GC (Monitoring of Pesticides in the Waste Water from Golf Course)
View here (169 KB)

Analyses for Pesticides with Capillary Column (Monitoring of Pesticides in Tap Water)
View here (160 KB)

Analysis of Pesticides on Golf Course with a Capillary Column (Monitoring of Recidual Pesicides in Tap Water)
View here (125 KB)

Analysis of Residual Pesticides in Agricultural Products
View here (158 KB)

Analysis for Pesticides with Capillary GC (No.2) -Simultaneous Detection Using Three Detectors; ECD,FPD and FTD-
View here (154 KB)

Application of Capillary GC with uTCD on GC-17AAT Ver.3
View here (132 KB)

High Speed Analysis with Narrow Bore Columns on the GC-17A Ver.3
View here (148 KB)

Packed Column Analysis of Gas Mixture Using the GC-17AAT
View here (114 KB)

High Sensitivity Analysis by Large Volume Injection Method with PTV (Programmed Temperature Vaporizer)
View here (172 KB)

High Sensitivity Analysis by Multiple Large Volume Injection Method with New Automatic Injector AOC-20i
View here (168 KB)

Analysis of organic Tin Compounds with Capillary GC-FPD
View here (32 KB)

Agreement between GC and GCMS Chromatograms (Chromatograms obtained at same linear velocity using AFC)
View here (67 KB)

Analysisi of acrylamede in tap water using GC-ECD
View here (60 KB)

Improvements of Saparation with the GC-2010 Constant-vleocity Mode
View here (85 KB)

Easy Analysis of Residual MTBE in Environmental Water and Soil
View here (121 KB)

Analysis of Residual Solvents in Pharmaceuticals (Part 5) Comparison of Headspace GC Sensitivity when Using Different Dilution Solvents
View here (113 KB)

Analysis of Gasoline and Alcohol using PONA System
View here (170 KB)

Reduction of Analysis Time (Part 3)
View here (86 KB)

Reduction of Analysis Time in Capillary GC (Part 4) Factors Affecting Analysis Time (Isothermal Analysis)
View here (25 KB)

Reduction of Analysis Time in Capillary GC (Part 5) Factors Related to Analysis Time (Analysis with Temperature Program)
View here (36 KB)

Analysis of Pesticides in Agricultural Product using ECD-2010 or FPD-2010
View here (78 KB)

Analysis of Residual Pesticides in Agricultural Products using ECD-2010 (Part 2)
View here (49 KB)

Analysis of Residual Pesticides in Agricultural Products using FPD-2010 (Part 3)
View here (66 KB)

Analysis of Residual Solvents in Pharmaceutical Products (Part 6) Comparison of Headspace GC Sensitivity depending on Dilution Solvents (Class 3 Solvents)
View here (138 KB)

Residual Solvents Analysis in Pharmaceutical Products (Part 7) - Thermostatting Dependencies of Aqueous Solution /Water Dilution Effect on DMSO Solution
View here (71 KB)

Use of Open Tubular Capillary Column (Guard Column)
View here (81 KB)

Analysis of Sulfur Compound in Gasoline by GC-FPD Optimum Initial Column Temperature in Splitless Injection Analysis Using Various Solvents (Part 2)
View here (82 KB)

Convenient Functions of GC-2014
View here (119 KB)

Introduction of a Multi-Detector GC System
View here (38 KB)

Improving the Repeatability of Aqueous Solution Analysis Performed with Capillary Columns
View here (87 KB)

Analysis of Thiophene in Benzene by GC-FPD
View here (111 KB)

Analysis of TPH in Soil (Part 1)
View here (67 KB)

Determination of Free and Total Glycerin in Biodiesel Methyl Esters -ASTMD6584 and EN14105-
View here (134 KB)

Analysis of TPH in Soil (Part 2)
View here (83 KB)

Analysis of 37 FAMEs using 6 Types of Capillary Columns
View here (99 KB)

Introduction of a Multidimensional GC SystemAnalysis of 37 FAMEs using 6 Types of Capillary Columns
View here (67 KB)

Easy Meaurement of the Distribution of Boiling Points for the Fractions of Petroleum by the Gas Chromatograph (Distillation Gas Chromatography)
View here (4812 KB)

Identification of brominated flame-retardants in polymers - FTIR-Spectroscopy - Method for compliance with the RoHS directive
View here (410 KB)

Kidneystones analysis by FTIR
View here (286 KB)

Double stitched provides more strength - Production control of fibres using FT-NIR
View here (547 KB)

Increased Productivity with NIR-FTIR technology
View here (426 KB)

Flower Power- GC for quality control of Biodiesel
View here (264 KB)

Fast GC-ECD Analysis of organochlorine pesticides
View here (1257 KB)

Enjoy without guilt
View here (215 KB)

Pyrolysis GC for thermal cracking feedstocks evaluation
View here (323 KB)

Sensitive nose provides more Safety in the Workplace
View here (159 KB)

Tank up in the rapeseed fields - Determination of glycerine, methanol, mono-, di- and triacylglycerides
View here (730 KB)

All roads lead to Rome - Fast LC -How
View here (501 KB)

Analysis of antidepressants - Pathfinder HPLC column technology
View here (963 KB)

Analysis of antidepressants - Pathfinder HPLC column technology
View here (963 KB)

Advanced Method Development, overnight
View here (1123 KB)

Tested and approved!
View here (176 KB)

Good day, sunshine
View here (138 KB)

Screening for bioactives compounds by means of ESI-MS
View here (220 KB)

Bisphenol A-consumption inevitable - Fast determination of bisphenol A residues in plastic bottles, containers and cans
View here (196 KB)

A good connection - Integrated in an online system: sample preparation and HPLC-MS analysis of biological samples
View here (339 KB)

Separation of Tricyclic Antidepressants Using Pathfinder Columns at Acidic Conditions
View here (217 KB)

LC Separations under 100% Aqueous Conditions using Pathfinder Columns
View here (162 KB)

Determination of Cimetidine in Pharmaceutical Tablets by High Performance Liquid Chromatograpy
View here (121 KB)

Mapping the Hydrophobic Character of Pathfinder Phases
View here (146 KB)

A Simple HPLC Method for the Detection of Preservatives in Inks
View here (118 KB)

Separation of Nucleotide Acid Bases with Reversed Phase Chromatography under Aqueous Mobile Phase Conditions
View here (122 KB)

Peptide Separation Using a Polymer Encapsulated Reversed Phase Column
View here (145 KB)

Steric Selectivity of Pathfinder Columns According to Tanaka and Enhelhardt
View here (139 KB)

Controlling Selectivity by Means of Temperature in Reversed Phase Chromatography
View here (144 KB)

Simultaneous Determination of Thirteen UV Filters in Sunscreen Products
View here (131 KB)

Selectivity of the Fluorescence Spectroscopy using a known mixture made from Anthracene and Naphthalene
View here (139 KB)

UV-Vis Application - UV Talk Letter - (1) Integrating Spheres (2) Solar Transmittance/Solar Reflectance Measurement V5 C101-E117
The EZ Test Shimadzu Texture Analyzer is used for various purposes such as numerical conversion of texture characteristics of foods such as chewiness, firmness, and palatability; quality evaluation based on the change of hardness; and strength evaluation of food packaging. The analyzer is usable for such a wide range of purposes because a wide variety of jigs can be selectively used according to the type of test being performed.
View here (823447 KB)

MALDI-Resonance Application - Phosphorylation Analysis by MALDI-TOF MS (5) -Identification of a Phosphoprotein (Japan)
Here we introduce an example of the analysis of 6-gingerol and 6-shogal, constituents of ginger, using the Prominence UFLC ultra fast LC system with the SPD-M20A photodiode array detector.
View here (1427444 KB)

Prominence Application - High Speed, High Resolution Analysis (Part 33) Analysis of Gingerol and Shogaol in Ginger
Integrating spheres are frequently used for measurement of solid samples using a UV-VIS spectrophotometer. These integrating spheres are introduced here.
View here (1449226 KB)

AA Application - Determination of Cadmium in Brown Rice by Flame Atomic Absorption Spectrometry
Here we introduce a method for enrichening phosphorylated peptides using titanium dioxide, as described in “phosphorylation Analysis by MALDI-TOF MS (3)” using the AXIMA Resonance combined with the μFocus plate.
View here (1097970 KB)

MALDI-Resonance Application - Phosphorylation Analysis by MALDI-TOF MS (4) -High-Sensitivity Measurement of a Phosphorylated Peptide (Japan)
Here we introduce examples of high-sensitivity analysis of bisphenol A using the Prominence RF-20Axs fluorescence detector by direct injection and by using an automated column-switching pre-concentrating system.
View here (1366426 KB)

GC Application - Analysis of Vinyl Chloride in Polyvinyl Chloride Plastics by GC
This Application News introduces an example of analysis of vinylidene chloride in plastic with polyvinylidene chloride as its principle ingredient.
View here (1022505 KB)

GC Application - Analysis of Vinylidene Chloride in Polyvinylidene Chloride Plastics by GC
Here we introduce examples of quantitative analysis of carbohydrates in foods using post-column solvent addition.
View here (1023511 KB)

Prominence Application - Applications Using "Prominence RF-20Axs" Fluorescence Detector (Part 6) Analysis of Bisphenol A at High Sensitivity
Here we introduce examples of analysis of cadmium in standard reference materials of powdered brown rice using the typical flame AA method, in addition to a high-sensitivity method using an atom booster.
View here (1375471 KB)

LCMS-2020 Application - Quantitative Analysis of Carbohydrates by LC/MS (Japan)
Here we introduce an example of high-sensitivity measurement of phosphorylated peptides using the AXIMA Resonance combined with a μFocus plate.
View here (1219813 KB)

MALDI-Assurance Application - RNA Sequence Analysis using the Acid-Hydrolysis Method (Japan)
This application News introduces an example of analysis of vinyl chloride in plastic with polyvinyl chloride as its principle ingredient.
View here (1446050 KB)

LCMS-8030/8040 Application - Screening of Sulfa Drugs Using the LCMS-8030 Triple Quadrupole Mass Spectrometer (Japan)
Japan’s Ministry of Health, Labour and Welfare has designated simultaneous analysis using C18 solid-phase extraction and LC/MS to be the official analytical method for managing targeted pesticides in water (revised on April, 2008). Currently, 31 pesticides are covered. This document describes simultaneous MRM analysis using polarity switching with electrospray ionization (ESI) and LC conditions for 30 of these targeted pesticides (excludes Oxine-Cu). The LCMS-8030 is capable of setting the MRM measurement time of each compound independently. In addition, the polarity switching time is short (15msec), making the LCMS- 8030 suitable for simultaneous analysis of multiple compounds that ionize in both positive and negative modes.
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LCMS-8030/8040 Application - Simultaneous Measurement of Positive-Negative Ions of 30 Pesticides for Water Management Using the LCMS-8030 Triple Quadrupole Mass Spectrometer (Japan)
Here we investigated RNA sequencing analysis using the acid-hydrolysis method.
View here (1508144 KB)

Measurement of Boiling Point Distribution (Simulated Distillation by GC) Internal Standard Method
View here (4296 KB)

Variable Angle Absolute Reflectance System for SolidSpect-3700
View here (70 KB)

Protein Thin Film Analysis by Reflection Absorption Spectroscopy and Single Reflection ATR
View here (58 KB)

Analysis of Ethanol and Anhydrous Ethanol in Accordance with the Fifteenth Edition of the Japnese Pharmacopoeia
View here (78 KB)

Analysis of Polyethylene Terephthalate by TG-FTIR
View here (323 KB)

Analysis of Protein Secondary Structure Using FTIR
View here (55 KB)

Analysis of Hydroxyl Value of Polypropylene Glycol Using the PLS Method in Near-Infrared Spectroscopy
View here (29 KB)

Analysis of Colored Components on the Surface of Tablets
View here (121 KB)

Analysis of Iodine Value with NIR Heated Transmission Cell and PLS Method
View here (86 KB)

Analysis of Silicon Surface by Single Reflection ATR
View here (39 KB)

Considerations in KBr Pellet Method (Part 2) Samples those require attention when analyzed by KBr method (Amino Acids)
View here (67 KB)

Screening Analysis of Brominated Flame Retardantswith ATR Accessory (Part 3) - RoHS Directive-
View here (25 KB)

Screening Analysis of Bromated Flame Retardants with ATR Accessory (Part 2) -RoHS Directive-
View here (300 KB)

Analysis of SiO2 Thin Film on Si Wafer
View here (59 KB)

Screening Analysis of Brominated Flame Retardants with ATR Accessory (1) (RoHS Directive)
View here (145 KB)

NIR(Near Infrared) Mapping using IR Microscope
View here (211 KB)

Applying Near Infrared Spectroscopy to the Identification of Drugs
View here (186 KB)

Pretreatment Methods for Eliminating the Interference Fringes
View here (46 KB)

The New Technique of Atmospheric Correction and its Effectiveness for FTIR
View here (64 KB)

Micro Contaminants Analysis with Infrared Microscope Micromanipulator System
View here (289 KB)

Measurement of layer-by-layer self-assembly film using RAS-8000 grazing angle specular reflectance accessory
View here (60 KB)

Applications of the DuraSampIIR employing a KRS-5 focusing element
View here (30 KB)

Quantitative analysis of cis, trans, and vinayl in polybutadiene using the single-reflection ATR accessory
View here (448 KB)

Automatic Diffuse Reflectance Attachment for Identification Application
View here (107 KB)

Applications of a New Single Reflection ATR Accessory
View here (157 KB)

Analysis of paint scrapings using an infrared microscope
View here (81 KB)

Quantitation of Phosphorus in a Silicon Wafer Using the PLS (Partial Least Squares) Method
View here (197 KB)

Applications of ATR Microspectroscopy
View here (459 KB)

Composition Analysis of Thin Film by Atomic Absorption Analysis - Determination of indium, antimony, tellurium -
View here (115 KB)

Determination of Metal Elements in Montmorillonite by AAS
View here (117 KB)

Measurement of Metal Elements Using the HVG-1
View here (199 KB)

Determination of Elemental Metals Using the HVG-1 Hydride Vapor Generator (No.2)
View here (90 KB)

Atomic Absorption Spectrophotometry of Plants Using a Hydrochloric Acid Extraction Method"
View here (110 KB)

Measurement of Trace Elements by Graphite Furnace AAS -Determination of Vanadium, Cobalt and Copper in Steel-
View here (115 KB)

Analysis of Aluminum Alloy by Computer Controlled AA-6500 AAS
View here (125 KB)

Determination of Metal Elements in Organic Solvents by Electrothermal Atomization Atomic Absorption Spectrometry
View here (124 KB)

Analysis of Commercially Available Standard Materiasl by AA-6700
View here (152 KB)

Analysis of Environmental Samples Using AA-6200
View here (152 KB)

How to Select Graphite Tube Types for the Electro-thermal Atomization
View here (143 KB)

Measurement of trace elements in beverage using furnace atomatic absorption
View here (118 KB)

Estimation of uncertainty in flame atomic absorption spectrophotometry
View here (118 KB)

Reflective LCD Cell-Gap Measuring System Using MultiSpec-1500
View here (434 KB)

Matrix modifier for furnace AA
View here (194 KB)

Measurement of Litium (Li) and Aluminium (Al) in Human Sera
View here (50 KB)

Stability of flame measurements with an autosampler
View here (122 KB)

How to caldulate the detectionlimit and the quantification limit in the furnace method
View here (177 KB)

Determination of Arsenic Species in Hot Springs
View here (125 KB)

Speciation of Arsenic in Blood by Cold Trap Vapor Reduction Atomic Absorption Method
View here (33 KB)

Analysis of Metals Contained in Soil
View here (24 KB)

Pb Analysis by Atomic Absorption
View here (43 KB)

Cd analysis by Atomic Absorption
View here (96 KB)

Zn Analysis by Atomic Absorption
View here (136 KB)

Measurement of Chromium in Soil by Atomic Absorption Spectrophotometry
View here (37 KB)

Na Analysis by Atomic Absorption
View here (186 KB)

Mn Analysis by Atomic Absorption
View here (131 KB)

Determination of Zn in River Water
View here (363 KB)

Potassium Analysis by Atomic Absorption
View here (62 KB)

Measurement by Flame Micro Sampling Method
View here (139 KB)

Ni Analysis by Atomic Absorption
View here (96 KB)

Al Analysis by Atomic Absorption
View here (94 KB)

Mercurry(H8) Analysis by the Electrothermal Atomization Method
View here (114 KB)

Assay of Nutriment in Processed Foodstuffs by Fluorescence Spectrophotometer and Atomic Absorption Spectrophotometer
View here (112 KB)

Caliblation curves of Standard Solutions Using AA-6200
View here (111 KB)

Analysis of Effect on Water Quality due to Leaching from Water Works Equipment Materials using the TOC-VCSH
View here (100 KB)

Cleaned Flue Gas Analysis of an Incineration Plant by TOC
View here (356 KB)

Conditiioning TOC Catalysts
View here (377 KB)

Control of Oil Contamination in Aqueous Cleaning Equipment using the TOC-5000A
View here (95 KB)

Determination of Calcium Carbonate in Secondary Cement Products using the TOC-5000A Solid Sample TOC Analysis System
View here (148 KB)

Determination of Organic Carbon in Ultra Pure Water
View here (424 KB)

Determination of the TOC Content in Landfill Leachate
View here (324 KB)

Determination of the Total Organic Carbon(TOC) in Solid Samples
View here (336 KB)

Determining theCorrect Concentrations of TOC in Purified Water and Water For Injection
View here (43 KB)

Evaluation of cleanliness of aluminum foil surface using the Shimadzu Total Carbon Analyzer
View here (248 KB)

Example of Management of Nitrogen Removal in a Night Soil Treatment Plant, using the On-Line Total Nitrogen Analyzer TN-4100
View here (355 KB)

High Precision Measurement of Total Carbon Dioxide in Sea Water
View here (99 KB)

Measurement of CO2 in an Ethanolamine Solution
View here (87 KB)

Measurement of Non-Purgeable Organic Carbon in Salt Water and Sea Water
View here (86 KB)

Measurement of TC and IC in Hydrogen Peroxide
View here (107 KB)

Measurement of TOC and TN in effluent using Shimadzu TOC-V combined with TN Unit
View here (306 KB)

Measurement of TOC and TN in Hydrogen Peroxide by TOC-VCSH Combind with TN(Total Nitrogen) unit TNM-1
View here (263 KB)

Measurement of TOC and TN Sea Water using TOC-VCSH with TNM-1
View here (1451 KB)

Measurement of TOC in Ammonia Solution by TOC-Vcsh
View here (243 KB)

Measurement of TOC in Bottled Mineral Water and Raw Water by TOC-VCSH
View here (227 KB)

Measurement of TOC in Chloroisocyanuric Acid using the TOC-VCSH
View here (287 KB)

Measurement of TOC in Copper(II) Sulfate Solution and Nickel Sulfamate Solution Using TOC-Vcsh
View here (235 KB)

Measurement of TOC in Electroplating Solution Using TOC-VWS
View here (360 KB)

Measuring of Organic Components in Fiberglass Using the TOC-5000A Solid Sample TOC Analysis System
View here (184 KB)

On-line TOC Measurement in the Food Industry
View here (388 KB)

POC measurement with TOC 4000 to prevent explosive gas mixture in closed waste water system
View here (62 KB)

Recovery Test for Sodium Dodecylbenzenesulfonate by TOC-VWS
View here (195 KB)

Reliable Online-Determination of TNb
View here (24 KB)

Shimadzu TOC-V Series, TOC Measurements on Tap Water, TOC Adopted as Water Quality Standard for Tap Water
View here (395 KB)

Sugarsweet campaign using the TOCN-4100
View here (15 KB)

Swab Method for TC-Determination of polluted surfaces
View here (45 KB)

TOC Analysis for Purified Water, Water for Injection and Clean-In-Place Applications (System Suitability Test)
View here (72 KB)

TOC Analysis of Drinking Water
View here (99 KB)

TOC Determination by the Addition Method. TOC=POC+NPOC
View here (346 KB)

TOC Determination in Sludge
View here (50 KB)

TOC Measuring of Boiler Water with Shimadzu's On-line TOC Analyzer
View here (127 KB)

TOC Measuring of Electroplating Solution with TOC-5000A
View here (113 KB)

TOC Measuring of Etching Solutions for Etching Process by using the TOC-5000A
View here (395 KB)

TOC Online Management of Collected Reusable Water In a Water Network Washing System
View here (123 KB)

TOC System Suitability Test of USP by Combustion Catalytic Oxidation TOC-VCSH
View here (128 KB)

Total Carbon Measuring in the Gas samples With the TOC-5000A Gas Sample Measuring Kit
View here (283 KB)

Total Organic Carbon (TOC) Measuring of Compost with the TOC-5000A Solid Sample TOC Analyzer
View here (166 KB)

Using Standard 40ML VOA Vials with Your Shimadzu ASI-5000A
View here (60 KB)

Waste water management in accordance with ISO 14001 using the Shimadzu On-Line Total Organic Carbon Analyzer
View here (611 KB)

Water management in the manufacturing process of Pharmaceutical products with TOC-5000A
View here (190 KB)

Water Quality Control at Water Purification Plants and Water inlets on Rivers Using the Online TOC Analyzer TOC-4100
View here (139 KB)

Application to Medical and Pharmaceutical Products (Melting Point and Fusion Heat)
View here (49 KB)

Application to Medical and Pharmaceutical Products of Multiple Crystallization
View here (51 KB)

Thermal analysis to be cited in the Japanese Pharmacopoeia
View here (43 KB)

Thermogravimetry in Hydrogen Atmosphere
View here (76 KB)

Analysis of Isothermal Decomposition with TGA
View here (72 KB)

High Sensitivity Thermogravimetry of Various Specimens
View here (63 KB)

Kinetic Analysis of Dehydration Reaction
View here (87 KB)

Measurement of Fusion Process of Nylon 66 Influence of Heat Treatment (Cooling Rate)
View here (64 KB)

Evaluation of Physical Properties of Thermosetting Resins
View here (71 KB)

Measurement of Water in Silica Gel with DSC
View here (74 KB)

Thermal Analysis of Rubber
View here (70 KB)

Application to Plastics (1) Floppy Disks
View here (62 KB)

Analysis of Isothermal Crystallization Process of High Polymers with DSC
View here (69 KB)

Measurement of High Polymer Materials with Cryogenic TMA
View here (80 KB)

Viscoelasticity Measurement of Polymer Films
View here (88 KB)

Measurement of Activation Energy Using Reaction Rate Analysis Program
View here (85 KB)

Measurement of Film Softening Temperature with TMA
View here (56 KB)

Specific Heat Measurement Using a Specific Heat Measurement Program
View here (62 KB)

Measurement of Bread Staling with DSC
View here (41 KB)

Stress-Strain Measurement of Polymer Film with TMA
View here (50 KB)

Measurement of Thermal Expansion of Floppy Disks with TMA
View here (47 KB)

Evaluation of Dangerous Materials Using Self-Reactivity Evaluation Program
View here (56 KB)

Glass Transition of Polyvinyl Acetate Type Adhesives
View here (59 KB)

Measurement of Partial Calories by Partial Area Measurement Program
View here (73 KB)

DSC of Waters in Several Gels
View here (50 KB)

DSC by Cyclic Heating/Cooling Program
View here (64 KB)

Measurement of Explosive Compounds by DSC
View here (56 KB)

Measurement of Phase Transitions of Liquid Crystals by DSC
View here (54 KB)

Measurement of Oxidizing Induction Time of PE by DSC
View here (62 KB)

Measurement of Reduction Process of Metal Oxides by TG Thermogravimetric Analysis
View here (59 KB)

The Transition Method of Plastic Temperature Measurement
View here (35 KB)

Measurement for Transition Heat of Plastics
View here (122 KB)

Fractionation by Preparative LC-MS and Fraction Verification byUltra Fast LC-MS
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Application using Analytical / Preparative LC-MS System (Part 1)
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Analysis of Perfluorochemicals (PFOA, PFOS) using LC-MS
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Ultra Fast Analysis (Part 2)Analysis of Impurities in Curcumin
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Determining Gemstone Authenticity and Precious Metal Purities by Energy Dispersive X-Ray Fluorescence Spectroscopy
Gemstones have been known to undergo various manipulations to enhance their perceived quality. As technology advances, confirming the authenticity of gemstones becomes more and more difficult, creating a large demand for a non-destructive, time-efficient method of determining the composition of gemstones and precious metals. One such method is Energy Dispersive X-Ray Fluorescence (EDXRF) spectroscopy, a technique that enables a user to quickly and easily differentiate between a true stone and a fraud or determine if any chemical enhancements have been made. In addition to stones, precious metals can be analyzed and quantified to identify their purities.
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AXIMA Series -Flexibility of Sample Targets
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AXIMA-TOF2-Low Mass Zoom. (LMZ)
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AXIMA Series-IntelliMarque. Automated Analysis Software Functionality
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AXIMA-TOF2-Improved Ion Gate for High Resolution Precursor Ion Selection
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AXIMA-TOF2 LC-MALDI Software Guide
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AXIMA Series-Oligo Analysis Software Functionality
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AXIMA Series-Sequence Calculator
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AXIMA-TOF 2-Protein Identification of Complex Mixtures using LC-MALDI on AXIMA-TOF2
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AXIMA-TOF 2 Simultaneous Protein Identification and Relative Quantification of Complex Mixtures using iTRAQ. methodology, LC-MALDI and AXIMA-TOF2.
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AXIMA - A Simple Alternative Sample Preparation Method for Improved Protein Identification from 1D
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AXIMA-TOF2 - Non-Linear Correction (NLC) Functionality to Improve Mass Accuracy in MALDI-TOF Analyses
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AXIMA-TOF2 - Analysis of Proteins in Linear Mode using an AXIMA-TOF2
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AXIMA-QIT - Investigating Rat Retinal Proteins Using a MALDI TOF MS
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AXIMA-TOF 2 - Protein Identification Using an Axima-TOF2
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AXIMA-TOF2 - SPECIFICATION TECHNICAL DATA
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AXIMA-QIT SPECIFICATION TECHNICAL DATA
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AXIMA-CFR.plus SPECIFICATION TECHNICAL DATA
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AXIMA - Characterization of Salmonella typhimurium membrane proteins separated by 2D gels
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AXIMA-QIT - The Analysis of N-linked Glycans by MALDI-QIT-TOF Mass Spectrometry
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AXIMA-QIT - Rapid Characterization of Small Molecules using MSn on a MALDI QIT TOF MS
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AXIMA-QIT - Characterization of Complex Protein Gel Bands by Offline LC-MALDI QIT TOF MS
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Improved ASTM-D3612 Method A TOGA System
Method A outlines the classical procedure for quantifying the gases contained within transformer oil. Labs that began performing this test on their transformers have many years of historic data that remains important today. This data is used to track the changing conditions within a specific transformer, and by monitoring gas levels, judgments may be made as to when preventive maintenance should be performed. This analysis method has been in use for many years and is known to have some areas of weakness. This document describes the technological advances in Gas Chromatography that are employed here to incrementally improve the original method.
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Controlling the Sulfur Content in Ultra Low Sulfur Diesel (ULSD) Fuels
Sulfur enters our atmosphere as gaseous sulfur dioxide through the burning of fossil fuels such as diesel. This sulfur dioxide or SO2 affects the environment, man-made structures, our health, and even the industries that generate the toxic gas. Sulfur dioxide combines with water vapor in the air to form sulfurous and sulfuric acid. These acids fall back to earth in water vapor and slowly devour away stone and cement edifices, statues, and ancient ruins around the world .
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Introduction of Contaminant Analysis Program
The upgraded version of IRsolution Ver. 1.40 that will be provided with the new Fourier Transform Infrared Spectrophotometer IRAffinity-1 is equipped as standard with the “Contaminant Analysis Program (Patent Pending)”.
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Volume 1 of UV Talk Letter
An information magazine for users of UV-VIS. Topics include: What is light?, Film Thickness Measurement, Q&A on Single-Beam and Double-Beam spectrophotometers.
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MDLs for Nitrogen Species by TrAAcS and Shimadzu
The method detection limits (MDLs) of high temperature oxidation (HTO); Shimadzu TOC-VCSH analyzer with TNM-1 TN unit) and wet chemical digestion (WCD); Bran & Luebbe TrAAcs 800 Autoanalyzer) were compared using various reagent grade organic chemicals containing nitrogen:
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Measuring Samples Per USP 26 Using Shimadzu TOC-VW
Shimadzu’s TOC-VW analyzer is perfectly adapted to meet the requirements of USP Rev. 26 method <643>. The TOC-VW analyzer utilizes three forms of oxidation (heat, UV, and persulfate) to provide a low maintenance instrument with a detection limit of 0.0005 mg per liter.
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Measurement of Polymer Materials by TMA
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Colourful, but hazardous: forbidden dyes - Fast C/MS determination of Sudan Red I-IV, butter yellow and Para Red in foods
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Make sure you know what you're dealing with! - Analysis of phenoxypropionic herbicides using LCMS
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An apple per day keeps the doctor away - Mycotoxins are a world-wide problem - Highly sensitive determination of the fungal toxin patulin
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Accute poisoning with a rat bait
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Organ transplantation - Determination of sirolimus and tacrolimus with HPLC/MS in whole blood
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Simultaneous determination of tryptophan, phenol, p-cresol and cholic acid in pretreated human blood
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Quantification of Acetylsalicylic Acid and ist Related Substance Salicylic Acid
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A Rapid Method for the Determination of Amiodarone and ist Metabolite Desethylamiodarone
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Routine determination of hazardous compounds
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Well, cheers - AAS in drinking water analysis
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AA-6300 for multi-element analysis with system validation
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The aroma of French fries - fuel from rapeseed oil
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EDXRF Analysis of Lead and Cadmium in Aluminum Alloy
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EDXRF Analysis of Cd, Hg and Pb in Blood
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EDXRF Analysis of P, Cl, K and Ca in Blood
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EDXRF Analysis of Chromium, Lead and Cadmium in Metals (Brass)
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EDXRF Analysis of Brewer's Yeast preparation
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EDXRF and WDXRF Analysis of Cd
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EDXRF Analysis of Cd and Pb
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Analysis of Ceramics
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The Content Matching Function
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Analysis of Food for Contamination
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Analysis of Food Products for contamination
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Analysis of Foreign Matter using CCD
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EDXRF Analysis of Glass
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EDXRF Analysis of Incinerated Ash
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EDXRF Analysis of Lead in Lead free solder Materials
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Analysis of low-alloy Steel
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EDXRF Analysis of Oil
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EDXRF Analysis of Orchard leaves
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Analysis of Pharmaceutical Products
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EDXRF Analysis of Chromium, Mercury, Bromine, Lead and Cadmium in Plastic Materials
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EDXRF Analysis of Polymer Film
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EDXRF Analysis of Precious Metal
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Analysis of Trace Elements in Water using Ultra Thin Film (1)
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Analysis of Trace Elements in Water Ultra Thin Film (2)
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EDXRF Analysis of Sulfur in Oil
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EDXRF Analysis of Thin Film
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EDXRF Analysis of Vehicle Exhaust Particulates
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Go for Gold? Layer thickness determination using EDX
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Go for Gold? Layer thickness determination using EDX
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Lead in lead-free solder - EDX-720
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Determination of hazardous substances according to RoHS - Energy-dispersive X-ray Fluorescence Spectrometer
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Brominated Flame Retardants-1 FTIR determination of brominated flame retardants following the rules of RoHS and WEEE using a single reflection accessory
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Brominated Flame Retardants (2) FTIR Determination of brominated Flame Retardants using an automated tool - RoHS Software
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Effect of resolution to vapor phase spectrum of HCL in the Infrared Spectroscopy
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Infrastructure of a circuit board - an observation of different layers made from NBR and cellulose
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Oil in water analysis under different European norms
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Measurement of different Sugar based samples like Sugar, honey, Syrup with FTIR Technique
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Fast determination of deuterated water in body fluids and natural waters
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Fluorescence library including Emission and Excitation Spectra from Pharmaceuticals and other materials
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Determination of Mercury in Water using Floureszenz Spectroscopy
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Flourescence quenching shown with the determination of Mercury (II) in combination with Bathophenanthroline
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Selectivity of fluorescence spectroscopy demonstrated on a pre-defined mixture of anthracene and naphthalene
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Cleaning Validation – Direct Swab method procedure using TOC-V and SSM-5000A
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Determination of TOC in solids according to the European Standardisation EN 13137
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Recovery rates of total organic compounds according to the European Standardisation EN 13137
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'Direct Swab' TOC of E. Coli cells in biopharmaceutical cleaning validation
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Determination of the TNb in the trace range (TOC-V CPH + TNM-1)
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Information on relationships between common parameters: COD, BOD, TOD and TOC
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TOC (+TNb) determination in seawater
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Determination of drinking water with TOC-V CPH
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Simple TOC determination of sediments and soils using the suspension method
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TOC determination according to USP 24 / EP 2.2.44 with TOC-V CPH
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TOC determination in Cleaning Validation
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Determination of waste water with TOC-V CPH by using the autodilution function
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Determination of the detection and determination limit of NPOC with the standard catalyst
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Calibration and measurement in the high sensitive range with TOC-V WP
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Determination of wine colour with UV-VIS Spectroscopy following Sudraud method
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Secrets revealed from water - TN determination in the ultra trace range
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Laboratory and online TOC measurement in paper production
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Environmental protection within our own hands - Continuous monitoring of wastewater of Shimadzu production sites using TOCN-4100 and AA-6300
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Around the clock operation: the TOC-4100 in wastewater treatment plants
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Online-Analysis at its best - Online TOC measurement in the food industry
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Sum parameters on trail - continuous TOC determination in environmental analysis
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TNb determination in the monitoring and control of municipal wastewater treatment plants
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TOC in the cement manufacturing process
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Drinking water even purer with TOC - European drinking water guidelines are now national law
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Flakes, fibres and particles - TOC in wastewater
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Successful participation in round robin tests TOC susension mehtod for sediments and soils
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Continuous measurement of ultra pure water - The TOC-V CSH online option
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CO2 determination in Beer - Fast help with TOC
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Blank value determination - TOC trace analysis
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Fine, finer, finest - TOC determination in ultra pure water
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Chromium (VI) analysis according to the RoHS guidelines - Hardware and software for accurate determination of hazardous substances
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Far away, but still within reach - UV-VIS measurement with fibre optics - Successful analysis using routine systems
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Measurement of Starch by DSC
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Determination of Asbestos in MortarAdmixture by TG-DTA
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Measurement of Fats with DSC
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Application of Photovisual DSC
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Measurement of Engine Oil by DSC-60+TAC-60L
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Computer-assisted Optimizatiuon in the Development of a HPLC method for the analysis for Amines
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Column Classification and Selection of Pathfinder stationary phases with embedded Polar Groups
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Realization of HPLC Methods by using Computer Assisted Method Development
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How Hazardous is your Shampoo?
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Swab test of E. Coli cells - Biopharmaceutical cleaning validation
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Quality assurance in water analysis - Determination of selected elements using ICP-OES
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Truth lies in the must- Must content as wine quality indicator -FTIR-Spectrosocpy
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Napoleon did not die from arsenic poisoning - ADAIS- Automated poison and drug analysis
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Which column is your analysis based on?
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Biodiesel Quality Control according to DIN EN 14105
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High Precision Diglycol determination with GC-2010
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Headspace Gas Chromatography with AOC-5000/ GC-2010: BETX in Water and aldehydes determination in printed colours
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The effect of Filter Time Constant in fast GC using the GC-2010: Application on kerosene analysis
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Determination of oil in water part II: Inection with OCI/GC-2010 ans SPL/GC-2010
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Using the Shimadzu GC System in the Fuel-Grade Ethanol Production Laboratory
In the case of the ethanol production plant, GC analysis is used to determine the total amount of denaturant added to the final ethanol product prior to shipment. This analysis is required in certain ethanol markets.
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Faster GC Analysis of Fuel Grade Ethanol
Ethanol production laboratories are familiar with gas chromatographic (GC) analysis of denaturated ethanol using ASTM Method D5501. This method is often implemented with various "convenience-based" modifications to determine the amount of ethanol, methanol, and total denaturant in the product. This method uses very basic instrumentation, by current standards, and requires that several post-analysis calculations be performed to arrive at values that are meaningful to the production laboratory.
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Using the HPLC System in the Fuel-Grade Ethanol Production Laboratory
High Performance Liquid Chromatography (HPLC) has become the most widely applicable method of chromatographic analysis. Through use of highly efficient column materials (the stationary phase), pumping system that provide a constant flow of the mobile phase, and detection components designed to accommodate the needs of analysis, HPLC is now a commonly used technique for monitoring the progress of the fermentation in the ethanol production laboratory.
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Benzodiazepine Analysis by UFLC
This application note details the separation and detection of four benzodiazepines with ultra-fast LC (UFLC) conditions.
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Analysis of Analgesics by UFLC
This application note focuses on the high-speed analysis of over-the-counter analgesics with Ultra Fast HPLC.
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Getting the Lead Out
This application notes focuses on Energy Dispersive X-Ray’s Role in Uncovering Dangerous Toys.
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High-Speed Analysis of Aldehydes and Ketones
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High-Speed Analysis of Lycopene
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High-Speed Analysis of Chlorogenic Acid
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High-Speed Analysis of Glycyrrhizinate
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High-Speed Analysis of Berberine
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High-Speed Analysis of Catechins
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High-Speed Analysis of Curcumin
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High-Speed Analysis of Artificial Colorant
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High-Speed Drug Analysis
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High-Speed Analysis of Furfural
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High-Speed Analysis of Pesticides in Water Quality Regulation
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High-Speed Analysis of Fat-Soluble Vitamins
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High Speed Analysis of Vitamin D
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High Speed Analysis of Phenolic Antioxidants
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High Speed Analysis of Organic Acids
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High Speed Analysis of Sennosides
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High Speed Analysis of Glycyrrhetinic Acid
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High Speed Analysis of Sulfa Drugs
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High Speed Analysis of Sudan Dyes
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High Speed Analysis of Polynuclear Aromatic Hydrocarbons (PDA Detection)
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High Speed Analysis of Synthetic Sweeteners
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High Speed Analysis of Vitamin B12
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High Speed Analysis of Hesperidin
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High Speed Analysis of Amino Acids by Pre-Column Derivatization (2)
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High Speed Analysis of Amino Acids by Pre-Column Derivatization (1)
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High Speed Analysis of Food Preservatives
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High Speed Analysis of &alpha;-Lipoic Acid
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High Speed Analysis of Scopolamine and Hyoscyamine
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High Speed Analysis of Coenzyme Q10
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High Speed Analysis of Chlorfluazurons
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High Speed Analysis of Chlorhexidine
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Measurement of Thermal History of Plastics by DSC
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Cooling Studies on Polymer Crystallisation
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Evaluation of Purity and Thermal Resistance of CNT by Thermogravimetry
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High-Speed Analysis of Malachite Green and Metabolites
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High-Speed Analysis of Ginsenosides
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High-Speed Analysis of Cortisone and Cortisol
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Selective lonization, Detection by MALDI-TOF MS with NBS-labeled Peptides using New Matrix
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Stable Isotope Labeling Method (NBS*) Protocol Improvement
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Detection of Phosphorylated Proteins by Chemical Dephosphorylation with Hydrogen Fluoride
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Western Blotting Method: Direct On-Membrane Identification of Target Molecules Detected by Western Blotting
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Direct Analysis of Microorganism Samples by AXIMA-CFR
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Efficient separation and accurate spotting of NBS Labeled Peptides Using the Micro HPLC AccuSpot System
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Protein Oxidative Modification Analysis using AXIMA-CFR
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Quantitative proteome analysis by labeling tryptophan residues (NBS Method)
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Chemical Method for Dephosphorylation of Phosphopeptides
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No. 10 Analysis of Polybutadiene using MALDI-TOFMS
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No. 9 Analysis of Polyethylene Glycol using MALDI-TOFMS
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No. 8 Polymethylmethacrylate Analysis using MALDI-TOFMS
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No. 7 Polystyrene (PS) Analysis using MALDI-TOFMS
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Analysis of Phosphopeptides using AXIMA-CFR
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Protein Identification by Library Search- Use of MS-Tag Method by Seamless PSD Analysis -
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Analysis Example of Porphyrin Metal Bond
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Identification of Phosphatidylcholine (Lecithin) using Seamless PSD Analysis
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Proteome Analysis Example using AXIMA
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Analysis of Linear Polysaccharide Chain using MALDI-TOFMS
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Analysis of Samples Containing S-S Bond using DAN
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Proteome Analysis Using the AXIMA-QIT
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Polymer Analysis by MS/MS Measurement - Polymethyl Methacrylate Fragmentation -
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Interaction MS Method: Direct On-Membrane Identification of Target Molecules Detected by Lectin Staining on Membrane
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Direct On-Membrane MS/MS Analysis using the Chemical Printer (CHIP-1000) and AXIMA-QIT
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AXIMA-QlT Analysis using FocusMass-T3 Enrichment Effect (1) Measurement of Mixture using High-Sensitivity MS/MS
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New Sialic Acid Stabilization Modification Method
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MS/MS Fragment Ion Coverage Improvement
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Top-down Proteomics using MALDI-MSn
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Identification of Phosphorylation Sites by AXIMA-QIT
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Sugar Chain Structural Analysis by MSn
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Analysis of a Glycopeptide
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Protein Identification by MS/MS Ion Search
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Analysis of In-Gel Digest Sample (1)- Protein Identification by Peptide Mass Fingerprint -
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Example of AXIMA-TOF2 Measurement using
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MS/MS Measurement of Phosphorylated Peptide using AXIMA-TOF2
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de novo Sequence Analysis of Internal Protein Fragment with ORFinder-N Mass Sequencing Kit
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AXIMA - Oligonucleotide Mass Analysis and In-Source Decay Sequencing on a MALDI TOF Mass Spectrometer
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AXIMA-QIT - Sequencing of Tryptic Peptides using a Novel CD Based Chemical Derivatization and a MALDI QIT TOF MS
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AXIMA-QIT - Analysis of high mass peptides using a novel Matrix-Assisted Laser Desorption Ionization Quadrupole Ion Trap Time-of-Flight (MALDI QIT TOF) mass spectrometer
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AXIMA - SNP analysis performed on a research grade MALDI-TOF mass spectrometer
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AXIMA -Parallel sample preparation in a CD microlaboratory to enhance protein identification using the AXIMA-CFR.plus
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AXIMA -Post Source Decay analysis of large peptides (>2,400 Da) and its value in protein identification
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AXIMA -Seamless PSD. using a high resolution curved field reflectron TOF mass spectrometer
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Comprehensive Evaluation of Polymer Electrolyte Membrane (PEM) Using Thermal Analysis
This Application News presents a comprehensive evaluation of a polymer electrolyte membrane (PEM), one of the important factors affecting the performance of fuel cells.
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Analysis of Polymers by MALDI-TOF Mass Spectrometry
Synthetic polymers are widespread in everyday life. Whether they are used as containers for household products and foodstuffs to the construction of car components and electronic devices, plastics now play important roles in our day-to-day activities.
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Prominence UFLC Next Generation High Throughput-HPLC
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Identification of Phospholipid molecular speices by Neutral Loss Survey and MS3 Analysis
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Structural Prediction of Impurities in Drugs using MSn Data
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Natural Product Analysis using the LCMS-IT-TOF
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Analysis of artificial colorants by ultra-fast liquid chromatography-mass spectrometry
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Principles and practical applications of Shimadzu's ELSD-LTII evaporative light scattering detector
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Migration points to fast analysis; Optimization of the system and analysis conditions
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Identification of unknown polymer additives using LCMS-IT-TOF
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Opening LCMS to research: Psiport browser software
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Development of a LC-QIT-TOF mass spectrometer capable of high mass accuracy and stability.
The combination of QIT-TOF with an ESI source has been developed using novel ion introduction optics. Instrument performance characteristics have now been extensively investigated in regards to mass accuracy and stability.
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Metabolite Profiling of Arabidopsis by Shimadzu LCMS-IT-TOF with Phenomenome Profiler software
Biological function is the sum of gene interactions and metabolic network interactions; both are affected by the environment and genetics. Metabolomics can detect cryptic changes and link unpredictable phenotypes to their biochemistry. With the Shimadzu LCMS-IT-TOF, we can acquire high resolution and high mass accuracy in the MS data to help identify novel metabolite discovery.
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Using the LCMS IT TOF to identify impurities in pharmaceutical candidates using high mass accuracy
In the development of pharmaceutical candidates it is critical to identify by-products of the reaction mixture. In this paper we describe the application of a novel hybrid instrument platform delivering high mass accuracy data and MSn analysis in identifying impurity products from a relatively impure pharmaceutical candidate.
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MSn analysis Identification of Molecular Species of Phospholipids by combination of Neutral Loss Survey and MS3
To elucidate the function of phospholipids, it is necessary to analyze not only their classes and subclasses but also molecular species. Recently, in the analysis of phospholipids, the application of mass spectrometry (MS) has become increasingly popular.
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Natural Product Analysis Utilizing an Ion Trap - Time-of-Flight Mass Spectrometer (IT-TOF)
Recently, the popularity of remedies consisting of natural products found in foods, roots, and herbs has increased in both the domestic and global healthcare markets. As a result of this renewed focus on natural remedies, efficient identification and analysis of the active compounds in these products is a growing area of method development. The LCMS-IT-TOF allows researchers in this field to obtain both chemical and structural information as it utilizes both the fragmentation power of the ion trap and the high resolution, and mass accuracy, of a time-of-flight mass spectrometer.
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Structural Elucidation by Composition Formula Predictor Software Using MSn Data
Discerning the chemical formula or structure of unknowns is a difficult task which can be partially alleviated by acquiring high mass accuracy data; however, data interpretation is tedious and time consuming. By using fragmentation spectra collected from a novel hybrid ion-trap time-of-flight (IT-TOF) mass spectrometer along with enhanced software, samples are rapidly analyzed to identify chemical formulas and structures.
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Isotope modeling routines applied to empirical formula prediction
High mass accuracy mass spectrometry is achievable on a number of technology platforms including time of flight mass analyzers, hybrid ion trap and quadrupole time TOF&acute;s to FT-MS. Such technologies are playing an increasing role in pharmaceutical drug characterization and metabolite identification to help identify or confirm empirical formulae assignments. Recently, software tools have been applied to modeling theoretical isotopic distributions and mass accuracy data with experimentally derived data together with sets of chemical or statistical rules to help increase the probability of assigning the correct empirical formula.
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Precise Identification of Molecular Species of Phosphatidylethanolamine and Phosphatidylserine by Neutral Loss Survey with MS3 and Accurate Mass Measurement
To elucidate the function of phospholipids, it is important to analyze not only their classes and subclasses but also molecular species. We found that electrospray ionization (ESI) MS3 analysis is effective for more detailed and accurate annotation of each molecular species. We established the system for analyses of molecular species of phospholipids with neutral loss (NL) survey of the head group-relating mass values and succeeding MS3 analyses by selecting the resulting product ions as precursor ions for MS3 analyses (Fig.1).
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Metabolite Profiling of NSAID Effects In Vitro Using Shimadzu LCMS-IT-TOF System With Phenomenome ProfilerTM Software
Global metabolite profiling software was used to measure and compare the metabolic changes induced by several NSAIDs in HT29 cells and media. This approach can be used as a model system in monitoring drug metabolism in vitro. To help find components of biological significance the Shimadzu LCMS-IT-TOF was used to provide high mass accuracy MSn data integrated with Phenomenome Profiler software to integrate chromatography and mass spectrometry data sets together with statistical methods.
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Analysis of Biodiesel Blends by FTIR
Biodiesel fuel is a fuel for diesel engines that is derived from the fats and oils of natural life forms as the raw material. This fuel is receiving much attention as a means of helping to prevent global warming, and its practical use is being advanced in every country around the world.
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Fractionation of Anthocyanins by Preparative LC-MS System
Anthocyanins are the flavonoid color pigments present in all tissues of higher plants, including the petals and leaves.Besides their use as food dyes, anthocyanins have recently attracted attention due to their antioxidant properties.
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LC-MS Analysis of Carbohydrates Using Post ColumnAddition of Solvent for Improved Ionization Efficiency
Typically, in the HPLC analysis of saccharides, two different modes of chromatography can be used: aqueous normal phase, or ligand exchange mode. Due to the difficulty in completely separating all of the saccharides, these are used in a complementary manner. Since salts that are not easily volatilized cannot be used in LC-MS analysis, the suitability of the HPLC conditions for LC-MS analysis depends on the choice of the mobile phase. Because acetonitrile - water (or ammonium acetate in water) is generally used as the mobile phase in the aqueous normal phase mode, and only water is used as the mobile phase in the ligand exchange mode, these can be considered suitable mobile phases for LC-MS analysis. While these conditions are very suitable for the HPLC separation of mixed carbohydrates, there can be difficulty in observing an ion which up to then has been easily confirmed, or there may be a loss of sensitivity due to the separation column or the HPLC conditions.
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Comprehensive Evaluation of Polymer Electrolyte Membrane (PEM) Using Thermal Analysis
This Application News presents a comprehensive evaluation of a polymer electrolyte membrane (PEM), one of the important factors affecting the performance of fuel cells.
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Swab / Direct Combustion Analysis of Detergent Residue Using SSM-5000A
Cleaning validation with respect to production equipment at pharmaceutical facilities refers to the scientific verification that residues of previously processed products and detergent used for equipment cleaning, as well as foreign material from the environment in the production equipment, do not exceed specified permissible limits. HPLC (high performance liquid chromatography) is often used to conduct this evaluation, however, detection with HPLC is often difficult due to the large number of constituents in detergents, and further, measurement is not easy when preparation, such as sample enrichment, is required. On the other hand, if a TOC (Total Organic Carbon) analyzer is used, the residual amount of detergent constituents is easily detected because all of the carbon-containing substances are detected.The rinse method and swab method are tw
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Analysis of Polymers by MALDI-TOF Mass Spectrometry
Analysis of synthetic polymersby MALDI-TOF MS
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Analytical Instrument Validation According to Food Safety Management System (ISO 22000)
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Full Automation with Integrated LC
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Selecting Detectors for Compounds with No Optical Absorbance
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HPLC Techniques to Improve LCMS Performance and Productivity
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Prominence nano System and Applications
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The Determination of Hazardous Substances According to RoHS and WEEE Directives
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Simulated Distillation Analysis of Heavy Canadian Crude Oil by ASTM D 5307
Simulated Distillation is a GC technique for determining the boiling point distribution of a crude oil stock. This information is useful to refinery engineers in determining the process that most efficiently utilizes a feedstock of raw crude oil. This technique is also used to monitor the refining process to insure the products produced are as expected. When a feedstock oil contains components that are larger than C44 and which boil above 538ºC, ASTM D5307 becomes the analytical method of choice.
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Volume 1 of FTIR Talk Letter - How to Read Power Spectra (No. 1) - ATR Precautions (No. 1).
Main articles: How to Read Power Spectra (No. 1) , ATR Precautions (No. 1). - ATR Precautions (No. 1).
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Volume 2 of FTIR Talk Letter
How to Read Power Spectra (No. 2). ATR Precautions (No. 2).
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Volume 3 of FTIR Talk Letter
FDA 21 CFR Part 11 and Compliance for FTIR. Sampling Accessories Used for Microscope Measurement.
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Volume 4 of FTIR Talk Letter
FTIR Validation , Using Microscope Accessories.
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Volume 5 of FTIR Talk Letter
Why You Should Use Macro Programs. Microscope Measurement Techniques (Transmission Measurements).
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Volume 6 of FTIR Talk Letter
European Pharmacopoeia 5.0. Microscope Measurement Techniques (Reflection and ATR Measurements).
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Volume 7 of FTIR Talk Letter
FTIR Measurement Data Storage and Control. Thin Film Sample Measurement Methods and Precautions.
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Volume 8 of FTIR Talk Letter
Resolution and Aperture. Polarization Measurement of Film Using Single-Reflection ATR
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Volume 9 of FTIR Talk Letter
FTIR market situation in China. Mirrors Used with FTIR. Near-Infrared Region Measurement and Related Considerations Part 1
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Volume 10 of FTIR Talk Letter
Algorithms Used for Data Processing in FTIR. Near-Infrared Region Measurement and Related Considerations Part 2
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Introduction of Japanese Pharmacopoeia Program
The upgraded version of IRsolution Ver. 1.40 that will be provided with the new Fourier Transform Infrared Spectrophotometer IRAffinity-1 is equipped as standard with the formerly optional “Pharma Report Program”. The program, which enables simple identification testing according to the Japanese Pharmacopoeia, is introduced here in this Application News.
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Measurement Examples Using Polycapillary Parallel-beam Optics System
The term "polycapillary" indicates multiple (poly) fine glass tubes (capillaries) that guide X-rays. Xrays generated by a point source are accepted at a high solid angle by the polycapillaries, and emerge as a parallel beam at the opposite end. This optics system utilizes the X-rays generated by the X-ray tube more efficiently than the standard (concentrating) method (the Bragg-Brentano Method), to achieve higher diffraction X-ray intensities. The parallel-beam polycapillary method used by the optics system ensures that the angle of diffraction does not change due to displacements of the sample measurement surface.
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45° Peeling Tests on Leads of Surface Mounted Devices
45° peeling tests can be carried out on QFP and other gullwing leads. This test is applicable to not only conventional soldered joints, but also to the evaluation of lead-free solder, which is increasingly being used recently.
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Dynamic Displacement Measurements of Microactuators
Dynamic displacement of an actuator can be measured while applying a constant force. In measuring the relationship between the signal applied to the actuator and the displacement when moving a weight up and down using an actuator, the smaller the actuator the more difficult the displacement measurement becomes. Using the MST-I Microautograph, this kind of measurement can be carried out with the configuration shown below.
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Shear Peeling Tests of Surface Mounted Devices
Shear peeling tests of surface mounted devices (SMD) can be carried out. This test is applicable to not only conventional soldered joints, but also to the evaluation of lead-free solder which is increasingly being used recently.
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Tensile Testing of Individual Solder Balls
Using solder ball tweezers, tensile tests can be carried out on a single solder ball.
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Compression Tests with 250°C Heating and Positioning of Micro Specimens
The combination of a heating plate and positioning unit allows compression testing of micro specimens such as a single solder ball. The positioning unit also used in the DUH Micro Hardness Tester enables the compression point to be precisely pinpointed.
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Cyclic Compression Tests of Silicon Wafer Polishing Pads
As an example of cyclic compression test, the lifetime evaluation test for a silicon wafer polishing pad is introduced here. By carrying out cyclic compression tests on CMP (Chemical Mechanical Polishing) pads for silicon wafers, the difference in characteristics between old and new polishing pads was evaluated.
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Analysis of Barbiturates by UFLC
This application note details the separation and detection of seven barbiturates with ultra-fast LC (UFLC) conditions.
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Applications of Ultra Fast HPLC
This application note focuses on the high-speed analysis of xanthine derivatives and triarylmethane ink dyes with Ultra Fast HPLC.
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A Practical Approach to Conserving Acetonitrile
Shimadzu offers reasonable and practical analytical tools and solutions to the global acetonitrile shortage. The use of small particle columns with smaller column dimensions has become an increasingly popular way to reduce analysis times, and also provides the additional benefit of decreasing mobile phase consumption.
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LCMS-2020 - Reduces Limitations of HPLC Analysis and UFLC Analysis
As explained above, building an LC/MS system that employs an MS as an LC detector can effectively reduce the limitations of LC analysis. In addition to this, reducing those limitations to the greatest extent possible requires an MS that can handle ultra high speed analysis. The new “LCMS-2020” single quadrupole ultra-fast LC/MS, boldly addresses these requirements.
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Structural Analysis of Reserpine Degradation Products by LCMS-IT-TOF
Structural elucidation of impurities during the development of pharmaceutical products is very important. While NMR and other techniques can be used for this purpose, the mass spectrometer is the primary means of conducting structural analysis. In order to do this, high mass accuracy with MSn fragmentation data is absolutely necessary.
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Shimadzu’s Total Nitrogen Module Eliminates Environmentally Un-friendly Environmental Methods
For every two steps forward in technology we take one step back in terms of pollution. The same goes for monitoring the environment. For every test or method developed to identify and isolate toxins, a large amount of toxic waste is produced. Simply stated, environmental methods shouldn’t be harmful to the environment.
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LCMS-IT-TOF Analysis of Amoxicillin
Amoxicillin was successfully separated and detected under gradient conditions using a Shimadzu Prominence series LC coupled to the LCMS-IT-TOF. Data acquired on the LCMS-IT-TOF allows for MSn and excellent mass accuracy within one experiment. Mass accuracy data obtained from the LCMS-IT-TOF is comparable to data reported by other vendors requiring the use of an internal standard or a dual-sprayer configuration.
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Identification of Proteins by LC-MALDI System
This application note introduces that protein identification by MS/MS ion search using an LCMALDI system which combines a nano-LC system (Prominence nano-AccuSpot-AXIMA® Performance).
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Analysis of Minor Components in Synthetic Polymers Using SEC-AccuSpot-AXIMA System (1)
Shimadzu has developed the AccuSpot automated spotter to mix the eluate from the LC with the various reagents and directly load them onto the MALDI sample plate. As a result, an automated analytical SEC-MALDI analysis system was constructed that can rapidly simplify all the processing from LC separation to MALDI-TOFMS measurement.
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Oligosaccharide Analysis by MALDI-MS using Liquid Matrices
Oligosaccharide Analysis by MALDI-MS using Liquid Matrices
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Toy Analysis by Atomic Absorption
This application introduces the dissoultion testing of Pb from toy paint coating using the furnace atomic absorption method.
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Reducing Analysis Limitations by Using Multiple Detectors with Ultra Fast LC
Reducing Analysis Limitations by Using Multiple Detectors with Ultra Fast LC
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Volume 11 of FTIR Talk Letter
Safety of Windows and Prisms Used in FTIR, Points to Note in Rubber Analysis: Black Rubber, New Products
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Volume 2 of UV Talk Letter
The Structure of a Spectrophotometer, The Relationship Between UV-VIS Absorption and Structure of Organic Compounds, New Products: AA-7000
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Determination of Heavy Metals in Wine using simultaneous ICP-OES with Mini Torch
ICP-OES is the "state of the art“ tool for the daily routine in quality control of wine samples. The ICPE-9000 is flexible enough to prepare the system configuration in order to achieve calibration ranges as low as 1 to 10 ppb.
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Iron and Steel Analysis Using ICPE-9000
Here we introduce the analysis of iron and steel standard substances using the Shimadzu ICPE-9000 multi-type ICP emission spectrometer.
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High Speed Analysis of Explosives
Showcasing the development and use of a high-speed reversed phase method for monitoring explosive levels in soil samples near current and former munition plants, an important objective for both environmental and safety reasons.
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Reduced Solvent Consumption and Operational Costs with Existing LC Systems
Although the introduction of ultra-high speed LC is an effective way to conserve solvent, there is a very significant issue as to how this can be achieved with the existing LC systems in use. This report introduces detailed suggestions on how to most effectively reduce solvent consumption with existing LC systems.
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Analysis of Water Treatment Samples
Analysis of Water Treatment Samples with Shodex Columns.
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Analysis of Water Treatment Samples - Ammonium
Analysis of Water Treatment Samples with Ammonium using Shodex Columns.
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Anion Analysis of Ophthalmic Irrigation Solutions with the Shimadzu Prominence IC System
Ophthalmic solutions suitable for eye irrigation, eye strain relief, contact lens storage and tear replacement abound in the marketplace. While some of these serve as convenient agents for administering medicines in minute quantity, many are formulated to act as cleaning agents and may contain only dissociated ions like chloride or sulfate and organic preservatives such as benzalkonium chloride. The physiologic pH of blood and tears is approximately 7.4, but a pH range of 6.5 to 8.5 will prevent damage to the cornea. This is accomplished using buffers. To prevent eye tissue damage and maximize comfort, solutions are made to be iso-osmotic (equal osmotic pressure) with tears. The common term for this is isotonic. A 0.9% NaCl solution in pure water is said to be isotonic with tears. In this application note we will aid in the quality control of such ophthalmic solutions for their content of common anions.
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GPC-MALDI Analysis of Trace Components in Synthetic Polymers
Matrix-Assisted Laser Desorption / Ionization Time-of-Flight mass spectrometry (MALDI-TOF MS) is being employed in a growing number of synthetic polymer applications in recent years. Using this technique, monomer and terminal structures are predicted based on the masses of the observed polymer substance, molecular weight distribution is evaluated, and molecular structural analysis is conducted by MS/MS analysis.
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Analysis of Polymer Additives in Synthetic Polymers using a SEC-MALDI System
Synthetic polymers may deteriorate as a result of various factors such as light, heat, mechanical stress, electrical stress, radiation, chemicals and moisture. Various additives such as antioxidants, UV absorbent, HALS and thermal stabilizers have been developed to maintain the properties of polymers and prevent degradation. The amount of additive depends on the intended use of the polymer but, typically, it is less than 1% (w/w). Analysis of additives in synthetic polymers is therefore important for the evaluation of improvement of polymer properties. Recently, MALDI-TOF MS has been used for the analysis of polymer additives.
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Simplified Enrichment Method Using NeedlEx
Ambient odor analysis typically requires enrichment of analytes because odor thresholds are usually very low. Normally, a specialized device is required for enrichment and desorption during introduction into the GC; specialized devices often involve complex operation. The NeedlEx introduced here can be used to selectively enrich volatile organic compounds in ambient air by desorbing them into the enrichment medium packed inside the injection needle.
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Determination of Asbestos Content in Natural Brucite
Here we demonstrate that asbestos content can be assessed using the Shimadzu DTG-60 according to the above method.
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Phosphorylation Analysis by MALDI-TOF MS (3) Phosphopeptide Enrichment Technique Using TiO2
Over the past several years, great achievements have been realized in phosphorylation research due to research into specific phosphopeptide enrichment techniques using IMAC (Immobilized Metal Affinity Chromatography) and titanium dioxide (TiO2). Here we describe phosphorylation analysis using a combination of TiO2-based phosphopeptide enrichment and MALDI-MS/MS (seamless PSD).
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Phosphorylation Analysis by MALDI-TOF MS (2) Phosphopeptide Sequence Analysis Using Seamless PSD
In the CID method (Collision-Induced Dissociation) used in MALDI-MS/MS amino acid sequence analysis, phosphorylation site information is difficult to obtain due to the marked desorption of the fragile phosphate group caused by collision with the inert gas. However, when sPSD is used in the analysis, the obtained spectra clearly indicate some degree of suppressed phosphate desorption, which is attributed to the absence of inert gas.
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Phosphorylation Analysis by MALDI-TOF MS (1) Measurement Mode and Matrix Selection for Phosphopeptide Detection
Phosphorylation is one type of protein posttranslational modification (PTM) that plays a significant role in important biological functions. Mass spectrometry is recently being applied in phosphorylation interrogation, and various results have been reported. In almost all cases, however, the instrument configuration used has been based on electrospray ionization (ESI). Here we introduce a method useful for the detection of phosphopeptides by MALDI technique.
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On-Tissue Direct Analysis: Analysis of Changes in Amount of Lipids in Murine Hepatopathy Model due to Administration of Carbon Tetrachloride (CCl4)
Here we introduce an example of on-tissue direct detection of phospholipid changes using MALDI-TOF MS. The sample consisted of a mouse model hepatopathological tissue section in which injury was induced by administration of carbon tetrachloride, and on-tissue direct MS analysis was conducted at time intervals following administration of the carbon tetrachloride.
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Measurement of TOC in Sulfuric Acid Solution and Ammonium Sulfate Solution Using the TOC-VCSH
This Application News introduces examples of TOC measurement in 1 % sulfuric acid solution and in 1 % ammonium sulfate solution using the Shimadzu TOC-VCSH total organic carbon analyzer.
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Qualitative Analysis of Triglycerides in Cooking Oil Using GC/MS
The following examples illustrate the use of the combined data from EI and CI for the triglyceride analysis of a butter sample by GC/MS.
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Measurement of Quantitation Limits for Vitamin B12 and Caffeine in Aqueous Solution Using UV-1800 UV-VIS Spectrophotometer
Here we report on the measurement of vitamin B12 and caffeine in aqueous solutions using the Shimadzu UV-1800 UV-VIS spectrophotometer to determine their respective quantitation limits.
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Flash and Continuous Light Sources in Fluorescence
For fluorescence measurements from the ultraviolet through the visible and into the near infrared region, the typical light source is the Xenon arc lamp. This particular source has been widely used due to its favorable characteristics, primarily the ability to deliver high energy output over a wide wavelength range.
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Multi-Element Simultaneous Determination of Nutrients as well as Hazardous Elements in Brown Rice by ICPE-9000
This Application News introduces an analysis of a brown rice sample using the Shimadzu ICPE-9000 multitype ICP emission spectrometer, in which digestion of the sample into a solution was conducted using a microwave digester.
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Accurate Mass Measurement of High Concentration Samples by LCMS-IT-TOF
This investigation shows that ASC function is an effective tool for preventing loss of mass accuracy due to saturation of the detector. Using ASC, highly accurate measurement results can be obtained in a single analysis in cases where the concentration is unknown or when the sample contains a mixture of components at varying concentrations.
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Agent Report: Limiting Report Creation Risks Through Automatic Integration of Various Types of Data
This report entails how to limit report creation risks through automatic integration of various types of data. This report covers ensuring reliability of computer-acquired data, risks to analysis and test data, data alteration, difficulty of handling different types of data, integrating different types of data with agent report, ensuring a high level of security and agent report flexibility and extendibility.
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Open Solution: Open Access Environment by Web Browser
As described above, there are great advantages to be realized in an open access system comprising a closed network in a laboratory that is linked to an open network in a separate office environment. The introduction of Open Solution provides an open access environment which can be utilized to its maximum to achieve great efficiencies in LC and LC/MS analysis in the analytical laboratory.
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Structural Analysis of Industrial Materials by MALDI-MS/MS 01 - Analysis of the Organic Light-Emitting Diode (OLED) Polymers -
MALDI-MS/MS analysis using the high-energy CID technique is a simple analytical technique that provides abundant information due to the many product ions formed that reflect partial structures, as compared with the conventional PSD technique. This enabled examination of the side-chain structure in a polymer used for OLED displays.
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Analysis of Black Rubber Diaphragm by FTIR and EDX
Qualitative analysis of rubber can be conducted quickly and easily using ATR measurement. With this technique, an infrared spectrum is easily obtained for qualitative analysis by affixing a rubber sample to the surface of an ATR prism. However, the composition of elements in a rubber sample can also be analyzed easily and quickly, and further, non-destructively by EDX.
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Glass Plate Analysis in Accordance with JIS R3106
The methods for determining visible light transmittance, or simply visible transmittance, visible reflectance, solar transmittance, solar reflectance and solar energy absorbance, and normal emittance and their respective methods of calculation are specified in JIS R3106 “Testing Method on Transmittance, Reflectance and Emittance of Flat Glasses and Evaluation of Solar Heat Gain Coefficient”. Of these indices, which express the characteristics of plate glass, solar transmittance and its determination was introduced in Application News A396. The remaining plate glass indices are introduced here.
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Structural Analysis of Industrial Materials by MALDI-MS/MS 02 - Analysis of Nonionic Surfactants -
MALDI-TOF MS is a widely used analytical technique for the structural analysis of various industrial materials. In addition to monomer repeat unit mass information, partial structural elucidation can be achieved through MS/MS analysis by applying high-energy collision-induced dissociation (CID). Here we introduce an example of MS/MS analysis of a non ionic surfactant , a high surface-active agent which is used extensively in chemical and other industrial applications.
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Analysis of Aquatic Vegetation
The ICPE-9000 was used to perform quantitative analysis of aquatic vegetation (chlorella, sargassum). Nearly all of the elements agree with the certified values in the obtained results.
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Analysis of Fertilizer
Fertilizers classified as general fertilizers made from sludge are subject to official standards, including minimum content of principal ingredients, maximum limit for harmful constituents, etc., according to the Fertilizer Management Law. Therefore, it is necessary to perform quality assurance testing for the principal ingredients and harmful constituents before use as fertilizer. Here we introduce analysis of trace constituents in fertilizer using the multi-type ICPE-9000 ICP emission spectrometer. ICP emission spectrometry is highly sensitive and features a wide dynamic range, thereby enabling simultaneous analysis of these principle ingredients as well as toxic trace constituents.
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Analysis of Plants – 1
The multi-type ICPE-9000 ICP emission spectrometer was used to perform quantitative analysis of brown rice. The results obtained for many elements matched the certified values, including that for the trace element cadmium.
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Analysis of Plants – 2
The multi-type ICPE-9000 ICP emission spectrometer was used to analyze several plant standards (tea leaf, olive, citrus leaf). The results closely agree with the certified value for each element.
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Analysis of Plastic
An ICP emission spectrometer can be used to accurately measure low concentrations of harmful metals like lead and cadmium in plastics. Decomposition of plastic samples for analysis is conducted by the dry-ashing, wet decomposition or microwave digestion method. This application introduces the analysis of plastic using the multi-type ICPE-9000 ICP emission spectrometer.
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Analysis of Pond Sediment
The multi-type ICPE-9000 ICP emission spectrometer was used to analyze pond sediment. With the ICPE-9000, the semi-quantitation results can easily be obtained from the database (qualitative function), without generating calibration curves.
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Analysis of River Water
On May 30, 2003, the tap water quality standard was greatly revised in Japan, and this revision made notification of a new inspection method. Arsenic, selenium, antimony and uranium were newly added as items to be analyzed by ICP emission spectrometry, and measurement of all items except mercury became possible. There are many analytes specified in the analysis of tap water, from high concentration elements at ppm levels, like sodium and calcium, to trace elements at ppb levels, such as lead and cadmium. Accordingly, this requires an analytical instrument with high-sensitivity, a wide analysis range and high-throughput performance. This application introduces the quantitative analysis of tap water and river water using the multi-type ICPE-9000 ICP emission spectrometer.
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Analysis of Sewage and Sewage Sludge
This application introduces the quantitative analysis of sludge and sewage waste water using the multi-type ICPE-9000 ICP emission spectrometer. The ICPE-9000 is a highly sensitive ICP instrument equipped with the axial view as a standard function. By selecting the radial-view (option), simultaneous analysis of trace elements like cadmium, to high-concentration elements like iron, zinc and aluminum, etc. becomes possible. Moreover, as in the case of sewage sludge and waste water, even samples containing widely varying concentrations of the same elements can be measured simultaneously in a single batch.
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Analysis of Soil
Recently, along with redevelopment at old factory sites, etc., ground contamination with heavy metals and volatile organic compounds has become increasingly obvious. Due to this situation, the “Soil Contamination Countermeasures Law” was enacted on May 29, 2002 to protect the public health, and enforcement of the law began on February 15, 2003. Since it is probable that exposure to specific toxic substances over a long period of time results in high concentrations in the ground surface layer, and that these toxins enter the soil directly, maximum concentration standards were set for nine items. Twenty-five substances were specified as risks with respect to infiltration into underground water.
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Volume 12 of FTIR Talk Letter
Topics include: FTIR market amidst rapidly growing economy in India, Using infrared detectors- pyroelectric detectors, Foreign matter analysis by FTIR-handling spectra of foreign matter, Question on ATR objective mirrors.
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Blood Alcohol Analysis using the Shimadzu GC-2010 and AOC-5000 Headspace Autosampler
This application presents a static headspace method using Shimadzu GC-2010 and AOC-5000 headspace autosampler, which offers reliable qualitative analysis, reproducibility and short run times allowing for high throughput.
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Understanding RoHS What it Means to U.S. Manufacturers
With the Restriction of Hazardous Substances (RoHS) regulations scheduled to take effect in July 2006, there is ongoing concern regarding the readiness of American companies to meet new European standards. Initiated by the European Union, RoHS is a direct response to growing calls for the regulation of materials deemed hazardous (including lead, cadmium, and mercury) in consumer electronic equipment.
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Ca Analysis by Atomic Absorption
Here we introduced the analysis of Ca in talc according to the method prescribed by the Japanese Pharmacopeia, in which the addition of La is used in conjunction with the N2O-C2H2 flame.
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Swab / Direct Combustion Carbon Analysis of Drug Residue by TOC-VCPH
This Application News introduces an example of measurement of a drug substance using the TOC-VCPH Total Organic Carbon Analyzer with the SSM-5000A Solid Sample Combustion Unit.
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Tableware Analysis Using ICPE-9000
We introduce the analysis of commercial tableware using the Shimadzu ICPE-9000 multi-type emission spectrometer.
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LCMS Sample Evaluation Report
This report demonstrates the chromatographic performance and LCMS analysis for components of customer-provided samples. Utilizing the LCMS-2020, the objective was to attain resolution of 5 components from test mix in a total method runtime of 2 minutes or less, including MS spectral peaks.
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[ARCHIVED] LCMS Sample Evaluation Report - Roark Galloway & Miguel Gallego (SSI)
Two samples were submitted to the Shimadzu Southern California (SCA) regional laboratory to demonstrate chromatographic performance and LCMS analysis for components of those samples. The samples were prepared and provided by representatives from CONFIDENTIAL Research Laboratories for this evaluation. The samples were identified as: 1) Test mix (5 components) 2) Sample ID- A001
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AA Application - Determination of Lead in Sugar by Electrothermal Atomizatlion
When measuring the infrared spectrum of rubber that contains calcium carbonate, a large calcium peak masks peaks originating from some of the rubber constituents, which could cause difficulties in qualitative analysis. Here we introduce a pretreatment method for removing the calcium carbonate from rubber that contains calcium carbonate.
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MALDI-Confidence Application - Protein Sequencing Using In-Source Decay (Japan)
Here we present an example of high speed, high resolution analysis of phenolic acids using the Shimadzu Prominence UFLCXR ultra high-speed LC system with the SPD-M20A photodiode array detector.
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FTIR Application - Analysis of Rubbers by FTIR -Method to Remove Calcium Carbonate in Rubbers - No. A416
This investigation demonstrated that cis- and transisomers could easily be distinguished, even without the use of specific analytical standards, by comparing the temperature dependencies of the ECL’s.
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GC Application - Determination of Ethanol Content of Denatured Fuel Ethanol by ASTM D5501-04
This Application News introduces a fast analysis of acrylonitrile based on the use of the backflush GC system.
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GC Application - Chromatography of FAMEs Using Cyanopropyl Capillary Columns
This Application News introduces an example of analysis of a pseudo denatured fuel ethanol sample by gas chromatography according to ASTM D5501-04.
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GC Application - Fast Analysis of Acrylonitrile in ABS Polymer Using a Backflush GC System
Here we introduce an example of high resolution analysis of a BSA (Bovine Serum Albumin) digest*1 using an “LCMS-IT-TOF” combination system consisting of the Prominence UFLCXR ultra fast, high speed LC with the Shim-pack XR-ODS II high speed, high resolution column (particle size 2.2 µm) and an ion trap mass spectrometer (QIT)/time-of-flight mass spectrometer (TOF).
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Nexera XR Application - High Speed, High Resolution Analysis (Part 26) Analysis of Phenolic Acids
A fine porous polyolefin film is generally used as the separator in a lithium-ion battery, and serves to isolate the anode and cathode while preventing electrons from flowing directly between the anode and cathode, as well as to allow the electrolyte or ions to pass through. Accordingly, these properties are important with respect to the lithium-ion battery’s performance as well as safety. Here we introduce the physical properties test results using both DSC and TMA measurement.
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LCMS-IT-TOF Application - High Speed, High Resolution Analysis (Part 25) High Resolution Analysis of BSA Digest (Japan)
The MALDI-TOF mass spectrometer can detect ions that are formed due to fragmentation within the ion source (ISD, In-Source Decay). When ISD occurs in analysis of proteins, primarily it is the c-type ion (Fig. 1) of the N-terminus that is detected. Up to now, 2,5-dihydroxybenzoic acid (DHB) has been used widely as a matrix for inducing ISD, but recently, 1,5-diaminonaphtalene (DAN) has been reported to be a more effective matrix in this regard (see References). Amino acid sequence information is easily acquired by detection of ISD ions using DAN, and highly homologous proteins can be investigated using homology searches.
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Thermal DSC-60 TMA-60 Application - Measurement of Separator in Lithium-Ion Battery
To gain knowledge of microorganisms, diverse analysis technologies are applied to determine not only how many of what bacteria are where (identification and quantitation) but also for research into the response to bacterial components, bacterial-derived components, metabolites, and the environment. Shimadzu provides an extensive product range, expertise, and solutions to anyone dealing with microorganisms in a diverse range of fields, including medicine, foods, chemicals, energy, the environment, clinical medicine, and hygiene.
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UV-Vis Application - Accurate, Reproducible dsDNA Quantitation Using the Small-Volume BioSpec-nano Spectrophotometer
Here we introduce an example of such an analysis based on the method specified in the Japanese pharmacopeia.
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HPLC Application - Ultra High Sensitivity Analysis Using Newly Designed Fluorescence Detector
The BioSpec-nano is a state-of-the-art UV-VIS Spectrophotometer for life sciences, ideal for quantitation of nucleic acids or fluorescently-labeled nucleic acids. Completely automated, it offers such features as ‘Drop and Click’ sample mounting-measurement, and cleaning. Manual operations such as moving the optical fiber vertically or cleaning the parts in contact with the liquid are unnecessary. This communication presents data on the accuracy and reproducibility of the BioSpec-nano for dsDNA quantitation.
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Enhanced Productivity with a New Gas Chromatography System
A new gas chromatography system has been developed with technology improvements to enhance productivity. Analysis of hydrocarbon mixture C<sub>7</sub> - C<sub>33</sub> was used to demonstrate new design features. Excellent precision of retention times and detector response was demonstrated in all of the chromatographic results
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Determination of Lead in Sugar by Electrothermal Atomization
The Japanese pharmacopeia specifies that analysis of lead content in refined white sugar be conducted by the electrothermal atomization (furnace) method. Since high sensitivity sample analysis is possible even at micro levels, this method is effective for trace level analysis even for toxic elements other than lead. Here we introduce an example of such an analysis based on the method specified in the Japanese pharmacopeia.
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AA Application - Analysis of Na, K, Ca and Mg in Mineralwater using Microsampling Method
The AA-7000 in combination with the ASC- 7000 sample preparation station allows the automated flame micro sampling method. In this method, the flame atomic absorption analysis is conducted with small sample volumes (2 – 90 µL), while in the conventional flame method (hereafter “flame continuous method”), the sample is continuously aspirated with a flow rate of approximately 8 ml/ min and larger sample volumes are needed for aspiration.
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AA Application - Determination of Tl in Drinking
We have developed the ultra-sensitive fluorescence detector “RF-20Axs”. With a newly designed optical system to maximize light throughput, the RF-20Axs realizes the world’s highest sensitivity. More than 2000 of S/N ratio of water Raman scattering, and 1.5fg for limit of detection (LOD) of anthraceneare achieved. In addition to high sensitivity, the RF-20Axs has special features to enhance the reliability of analysis. Temperature control of both a flow cell and a photo-multiplier tube significantly improves the reproducibility of peak area. A high-speed data sampling rate (100Hz) realizes ultra-fast analysis without loss of separation.In this poster, we report additional application data indicating the remarkable performance of the RF-20Axs.
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High Speed Analysis of Explosives
A number of high-speed columns that employ the use of sub 2 micron particles have been fairly recently introduced and can be used for high-speed analysis of explosive compounds. Here, a high-speed explosives method was developed on a larger particle size fused core column that will provide a more rugged assay. Isocratic and gradient conditions were developed for a mixture of EPA 8330 explosive standards.
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Evaluation of UHPLC System Performance Under High Throughput Conditions
The use of UHPLC technology has continued to increase in LC applications as a way to reduce sample analysis turnaround times. A variety of applications in pharmaceutical and environmental areas with small particle columns will be presented that will demonstrate the throughput and performance that can be obtained with current UHPLC technology.
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Fast Analysis of Vitamins in Dietary Supplements Using LCMS
Utilizing an ultra-fast LCMS with high-speed scan capability and high-speed data acquisition to achieve good linearity and repeatability, as well as accurate quantitative analysis of water-soluble vitamins in dietary supplements.
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Evaluation of MS Scanning Speeds with Ultra Fast Liquid Chromatography
Accurate mass analysis of sharp chromatographic peaks obtained by high-speed LC requires ultra-fast MS detection capabilities. In this poster, a number of high-speed separations are evaluated with MS scanning speeds of up to 15,000 u/sec with polarity switching speeds of 15 msec.
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Protein Sequencing Using In-Source Decay
The MALDI-TOF mass spectrometer can detect ions that are formed due to fragmentation within the ion source (ISD, In-Source Decay). When ISD occurs in analysis of proteins, primarily it is the c-type ion (Fig. 1) of the N-terminus that is detected. Up to now, 2,5-dihydroxybenzoic acid (DHB) has been used widely as a matrix for inducing ISD, but recently, 1,5-diaminonaphtalene (DAN) has been reported to be a more effective matrix in this regard (see References). Amino acid sequence information is easily acquired by detection of ISD ions using DAN, and highly homologous proteins can be investigated using homology searches.
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Analysis of Rubbers by FTIR - Method to Remove Calcium Carbonate in Rubbers
Calcium carbonate, a white-colored powder with the chemical formula CaCO3, is widely used in many applications which can be found all around us. For instance, one of its most obvious applications is its use as the principal ingredient of chalk. However, calcium carbonate is also widely used in rubbers and plastics, paper and tooth powder, etc. as a filling material.When measuring the infrared spectrum of rubber that contains calcium carbonate, a large calcium peak masks peaks originating from some of the rubber constituents, which could cause difficulties in qualitative analysis. Here we introduce a pretreatment method for removing the calcium carbonate from rubber that contains calcium carbonate.
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Analysis of Food Preservatives by GC/MS
Food preservatives are used to prevent adverse changes and rotting of foods by suppressing the proliferation of microorganisms; they are used in accordance with the properties of the food to which they will be added. Many of the analytical methods used for analyzing food preservatives employ extraction of the compounds of interest by the steam distillation technique, followed by quantitative analysis using high performance liquid chromatography (HPLC). Gas chromatography can also be used for measurement of many compounds used as food preservatives, including benzoic acid, sorbic acid, methyl p-hydroxybenzoate (PHBA methyl), ethyl p-hydroxybenzoate (PHBA ethyl), propyl p-hydroxybenzoate (PHBA propyl), and butyl p-hydroxybenzoate (PHBA butyl), and therefore, gas chromatograph / mass spectrometer (GC/MS) is an effective analysis technique for verifying the detection results obtained by HPLC. This Application News introduces an example of GC/MS analysis of six types of food preservatives.
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Determination of Ethanol Content of Denatured Fuel Ethanol by ASTM D5501-04
In the United States, a denaturant is added to fuel grade ethanol, and the quality standard for this denatured fuel ethanol is specified in ASTM Method D4806. As part of this specification, D5501-04 specifies the measurement method and allowable concentrations of methanol as an impurity and ethanol as the principle ingredient. Furthermore, it also stipulates that gas chromatography be used for this determination of the methanol and ethanol content in denatured fuel ethanol. Separately, the moisture concentration in fuel is specified in D1364 and E1064, in which the moisture concentration is subtracted from each of the constituent concentrations obtained by gas chromatography to calculate the final methanol and ethanol concentrations. This Application News introduces an example of analysis of a pseudo denatured fuel ethanol sample by gas chromatography according to ASTM D5501-04.
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Chromatography of FAMEs Using Cyanopropyl Capillary Columns
Separation of the isomers of fatty acid methyl esters (FAMEs) by gas chromatography, and specifically the cis-and trans-isomers, is conducted by gas chromatography using strongly polar cyanopropyl capillary columns. Here we present our investigation of the chromatography of C18 fatty acid isomers using two types of columns, the BPX-90 and Rtx-2560. The structures of these two liquid phases are shown in Fig. 1.
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High Speed Analysis of Low Level Estrogens and Their Metabolites from Biological Samples
Introducing a novel multicolumn approach that uses an ultra-high pressure LC system for Cumulative Multicolumn Sequential Chromatography with different column chemistries for the required resolution, significantly reducing analysis time.
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Direct LC/MS Analysis of Basic Drugs in Plasma by On-line pretreatment with Weak Cation-Exchange Pretreatment Column
A new pretreatment column, Shim-pack MAYI-WCX (prototype), which has weak cation-exchange groups as stationary phase, has been developed for trapping of basic compounds in consideration of LCMS use. The retention ability and pretreatment effect of the Shim-pack MAYI-WCX was evaluated for desipramine, dibucaine, lidocaine, verapamil and amitriptyline by using a column-switching system (Co-Sense for BA system, Shimadzu).
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Fast Analysis of Acrylonitrile in ABS Polymer Using a Backflush GC System
This Application News introduces a fast analysis of acrylonitrile based on the use of the backflush GC system.
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AA Application - Atomic Booster
Flame atomic absorption spectrophotometry is an established inorganic analysis method for highly reliable routine analysis. Generally, flame atomic absorption spectrophotometry offers ppm-level measurements and furnace atomic absorption spectrophotometry offers ppblevel measurements. Furnace atomic absorption spectrophotometry offers superb sensitivity but requires drying, ashing, and atomization processes, which takes longer working time than flame atomic absorption spectrophotometry to offer the rapidity essential for routine analysis. The newly designed atomic booster enhances the measurement sensitivity of flame atomic absorption spectrophotometry and further enhances its range of application.
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Particle Size IG-1000 Application - Measurement of Nano Particles Used in Dye-Sensitized Solar Cell
The dye-sensitized solar cell features a simple construction consisting of a plate to which a transparent electrode coated with a thin layer of dye-adsorbed porous titanium oxide dye is affixed, and an iodide electrolyte solution. Low-cost mass-production can be realized because this type of solar cell does not require the large-scale equipment, which is necessary for conventional silicon-based solar cell manufacturing. Furthermore, this type of cell possesses features that the silicon-based solar cell does not have, specifically the ability to make batteries of various colors by adjusting the type of dye used, and the ability to produce flexible batteries by using plastic as the plate material.
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Particle Size IG-1000 Application - Measurement of Magnetic Nano Particles
In the field of medical treatment, iron oxide nano particles are used as a contrast medium for MRI. Nano particles that are about the size of a virus are taken up by cells where they function as a contrast medium. Since this function is jeopardized in such an application when the particle diameter increases due to aggregation of the nano particles, dispersibility in a liquid becomes important. In the biotechnology field, amino acids and proteins are bonded with magnetic nano particles, and this magnetization is used to efficiently collect specific proteins by collecting the bound magnetic nano particles. Decreasing the particle size causes an increase in the surface area of the particles, thereby increasing the efficiency of protein collection.
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Particle Size IG-1000 Application - Measurement of Pigmented Ink Nano Particles
The AA-7000 in combination with the ASC- 7000 sample preparation station allows the automated flame micro sampling method. In this method, the flame atomic absorption analysis is conducted with small sample volumes (2 – 90 µL), while in the conventional flame method (hereafter “flame continuous method”), the sample is continuously aspirated with a flow rate of approximately 8 ml/ min and larger sample volumes are needed for aspiration.
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Particle Size IG-1000 Application - Measurement of Nano Particles Used in Fuel Cells
A fuel cell is a kind of battery that continuously produces electric power from supplied oxygen and hydrogen. Water is discharged as a result of the reaction between hydrogen and oxygen. This highly efficient energy source, in which just a small amount of carbon dioxide, but no exhaust gas, is expelled, is gaining momentum towards wide-scale practical use.
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Particle Size IG-1000 Application - Measurement of Nano Particles Used in Sunscreen
Sunburn is caused by exposure to ultraviolet light in the medium wave (UVB, 280-320 nm) and long wave (UVA, 320-400 nm) ranges. UVB rays cause reddening and inflammation of the skin, as well as blistering, while UVA rays cause darkening and aging (wrinkles and sagging) of the skin. Ultrafine particles (nano particles) made of titanium oxide and zinc oxide, etc. are used in sunscreen to block exposure to ultraviolet rays. Titanium oxide has been demonstrated to be effective in shielding against UVB, while zinc oxide effectively screens out UVA rays. Furthermore, the use of such nano particles reduces the scattering of visible light rays, allowing formulation of sunscreens with high transparency that do not turn white upon application to skin.
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Particle Size IG-1000 Application - Measurement of Nano Particles Used in Vehicle Catalytic Converter
The three-way catalytic converter is installed in the exhaust system of gasoline-fueled vehicles to cleanse the emissions of toxic substances by oxidation and reduction reactions. The three-way catalytic converter is so named because it simultaneously cleanses the emission of three types of toxic substances, hydrocarbons (HC), carbon monoxide (CO), and nitrogen oxides (NOx). HC and CO are reacted with oxygen (oxidation reaction) to form water and carbon dioxide, while NOx is relieved of its oxygen (reduction reaction) to form nitrogen and oxygen. Precious metals such as platinum are used as the principal catalyst in the oxidation and reduction of these toxic substances. In addition, cerium oxide is used as a co-catalyst to enhance the action of the principal catalyst. The efficiency of the principal catalyst deteriorates when the concentration of oxygen in the exhaust gas fluctuates due to operational conditions. The cerium oxide co-catalyst assists the principal catalyst by storing excess oxygen in the vicinity, and releases this oxygen when it is deficient.
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Improved Transformer Oil Gas Analysis (TOGAS) by GC Headspace
A Gas Chromatograph configured with a headspace autosampler, two automated valves and three detectors is described, and the methodology from sample prep to GC analysis and data processing is examined. Improvements include detection limits for hydrogen, analysis of hydrocarbons up to n-C4 within 20 minutes, and automated software processing of the data to account for the actual temperature and atmospheric pressure.
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New Applications for Comprehensive Two Dimensional Gas Chromatography
Comprehensive two dimensional gas chromatography is a technique where all the analytes eluting from one chromatographic column are subsequently run on a second, dissimilar column to improve chromatographic resolution. This technique can significantly increase resolution in a complex mixture, and can be used to identify target compounds as well as provide a class separation. This poster provides some examples.
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Rapid GC Simulated Distillation Analysis
ASTM D 2887, simulated distillation (SIMDIS) GC method, is one of the most basic tests in the petroleum industry. In this method, retention times are directly correlated to boiling points and detector responses are correlated to the hydrocarbon concentrations. The contemporary petroleum industry continuously calls for high-speed analysis to improve productivity. Here, a new FAST SIMDIS GC technique, which reduces the run time from 45 min to 9.56 min, is presented.
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Improved Precision and Accuracy in Extended Natural Gas Analysis
Refinery gas analysis is performed to characterize the components created from a process gas stream. A newly developed system, allowing more precise and accurate results to be performed, has been developed. In this report, the retention time repeatability and quantitative results using this system are evaluated.
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New Applications for Multidimensional Gas Chromatography
Multidimensional chromatography is the utilization of multiple columns to enhance difficult separations. For a complex mixture there may not be a single column that resolves all the peaks of interest. A better separation may be possible using two columns of different polarity. In this poster, analysis examples using the MDGC/GCMS system, which utilizes a multi-Deans switch, are presented.
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Rapid Screening for Drugs of Abuse Using Higher Column Flow Rates in Gas Chromatography Mass Spectrometry
The need to run more samples in the same amount of time with the same data quality has increased; this poster addresses how certain modifications can be made to increase throughput while retaining high-quality data
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An Improved Method of Updating Retention Times for GCMS
A new technique has been developed to update retention times automatically in the data processing software. The technique is based on the use of retention indices, referenced to a hydrocarbon standard (series of n-alkanes). This is a multipoint correction method, so target analytes will always be bracketed by the retention index marker compounds to ensure accuracy of updated retention times for target compounds. Examples illustrate the accuracy of this technique using a subset of US EPA Method 8270D analytes.
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A New Generation of Capillary Advanced Flow Technology for Gas Chromatography Applications
The new GC-2010 Plus Advanced Flow Technology shortens analysis times and enhances chromatographic separations in complex matrices. In this study, single-oven MDGC separation of volatile aromatics from a gasoline sample was demonstrated. Multiple heart cuts were achieved without changing retention times of other peaks, enabling separation of peaks of interest from co-eluting matrix interferences.
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Nexera XR Application - High-Speed Analysis of Ultraviolet Absorbers (1)
In data sheet No. 66, a high-speed analysis example for 11 ultraviolet absorber components was shown. This data sheet shows a separation of representative four ultraviolet absorber components in shorter analysis time.
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Nexera XR Application - High-Speed Analysis of Ultraviolet Absorbers (2)
Polymer materials such as plastics contain a lot of additives to enhance the characteristics and functionality. This chromatogram shows a high-speed analysis of 10 polymer additives.
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Prominence Application - High-Speed Analysis of Polymer Additives (1)
In data sheet No. 68, a high-speed analysis example for 10 polymer additives was shown. This data sheet shows a separation of representative five polymer additives in shorter analysis time.
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Prominence Application - High-Speed Analysis of Polymer Additives (2)
This chromatogram shows results of a high-speed analysis of eight water-soluble vitamins.
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Prominence Application - High-Speed Analysis of Water-Soluble Vitamins
This Application News introduces the calibration curves for cadmium (Cd), copper (Cu), manganese (Mn), and lead (Pb) and the minimum limit of determination of the major elements when the booster is used.
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Nexera XR Application - High-Speed Analysis of Alpha Acids and Beta Acids in Hops
Many different types of UV-blocking cosmetic products are commercially available to protect the skin from UV rays. This data sheet shows results of a high-speed analysis of 11 ultraviolet absorber components.
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High-Speed Analysis of Alpha Acids and Beta Acids in Hops
Hops, a major ingredient in beer, contain components called alpha acids (humulones) and beta acids (lupulones). Through the brewing process, alpha acids are converted to iso-alpha acids (isohumulones) which are bitter components of beer. Beta acids are said to influence the bitterness balance. This data sheet shows results of a high-speed analysis of six alpha acids and beta acids.
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Volume Dimension and Number Dimension
The Nano Particle Size Analyzer SALD-7101, in which measurement is based on the laser diffraction method, measures particle size distribution using the volume dimension. However, if necessary, the volume dimension results can be converted into the number dimension particle size distribution for output of the results.
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Size Measurement of Microbubbles and Nanobubbles
Recently, there is great expectation that so-called microbubbles and nanobubbles will play ever-increasing roles in applications throughout various fields and for a variety of purposes, as shown in Table 1. Accordingly, research and development into methods and instruments for generating such bubbles are now underway. In fact, one could say that this trend is turning into somewhat of a boom. In part, there are already efforts to put these to practical use and to bring them to market. However, the reality is that various uncertainties still remain with respect to these bubbles, one of which is the matter of bubble size.
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Observation of Protein Antigen - Antibody Reaction
Applying the outstanding features of the Nano Particle Size Analyzer SALD-7101, which includes “measurement range: 10 m to 300 μm”, “shortest measurement time: 1 second”, and “measurement concentration range: a few ppm to 20%”, observations and measurements can be conducted with this instrument not only with respect to fixed particle distribution (unchanging), but also with variation (time-related behavior) in particle size distribution (particle diameter) due to a variety of conditions and influences. One application utilizing these features is the direct observation of the state of protein –protein interaction including antigen – antibody reactions, by observing changes in particle size as they occur without the use of labeling or other facilitating techniques.
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Evaluation of Agglutination in Vaccine and Proteins
With the Nano Particle Size Analyzer SALD-7101, laser diffraction method (static scattering method) is adopted as the measurement principle, allowing simultaneous measurement of the primary particles as well as clumps and contaminants using a single instrument. With this, the degree of agglutination in vaccines and proteins can be evaluated quickly and accurately. Moreover, time-course changes can be measured in 1-second intervals.
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Measurement of Needle-Shaped, Fiber-Shaped and Tubular Particles
Particle size distribution is normally expressed as particle amount (cumulative or differential) corresponding to a particle diameter scale. Using the Nanoparticle Size Analyzer SALD-7101 measurement principle “laser diffraction method”, particle size distribution is calculated based on the light intensity distribution pattern of the diffracted and scattered light, assuming that the particles are spherical in shape. Therefore, when using the SALD-7101, measurement is always conducted based on the assumption that the particles are always spherical.
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Evaluation of Sunscreen Cream
This application analyzes the particle size distribution measurement in sunscreen cream using the Nano Particle Size Analzyer SALD-7101.
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Size Measurement of Microbubbles and Nanobubbles – 2
Using the SALD-7101, the flow cell and bubble generator can be directly connected to the instrument to allow online, real-time bubble diameter measurement of generated microbubbles and nanobubbles, which allows the possibility of obtaining bubble diameter feedback.
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Evaluation of Particle Dissolution Process
Because the Nano Particle Size Analyzer SALD-7101 is capable of particle size distribution measurement at time intervals as short as one second, it is now possible to observe the time-course behavior of particle size distribution (particle diameter) during the dissolution process.
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Reason for Adopting UV Light Semiconductor Laser
When the particles to be measured are placed in a medium having a refractive index different than that of the particles, and which are then irradiated with laser light (collimated beam), scattered light is generated from the particles. In the “dynamic light scattering method (DLS)”, the time-course changes in scattered light intensity are utilized to measure particle size; however, in the “laser diffraction method” adopted in the SALD-7101, the special changes in light intensity (light intensity distribution pattern) dependent on the scattering angle is utilized to measure the particle diameter (particle size distribution).
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Measurement of Polystyrene Latex Particles
Using the IG-1000, we measured polystyrene latex particles which are widely used as standard samples. For measurement, we used polystyrene latex particles of label size (average size) 33nm to 97nm.
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Repeatability of Single Nanoparticles
Using the IG-1000, we measured commercially available silica particles of label size 5 nm to 25 nm containing single nano particles.
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Influence of Contamination
Using the IG-1000, we prepared a sample mixture of 1-μm particles added to 46-nm particles and performed measurement on the IG-1000 to look into the influence of large particles on measurement. We found that, when compared withnon-intrusion of large particles,there as hardly any influence caused by intrusion of large particles. This contamination resistance is one of the outstanding features of the IG method which uses diffracted light.
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Example of Measurement of Single Nanoparticles
On the IG-1000, we measured two types of silica particles (label size 5nm, 8nm) having different particle sizes both containing single nanoparticles and fullerene hydroxide particles. We found a distinctly apparent difference in the particle sizes of the two types of silica particles containing single nanoparticles. This difference between samples was clearly manifested also in the intensity data of the diffractedlight, the raw data.
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Measurement of Mixed Nanoparticles
The IG-1000 can accurately measure the particle size distribution of nanoparticles having a broad distribution, which has up till now been difficult by other methods.
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Measurement of Liposomes using the IG-1000
Here we introduce the results of measurement of liposomes using the IG-1000 nanoparticle analyzer , capable of measuring the size of a single nanoparticle.
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Measurement of Zinc Sulfide (ZnS) using the IG-1000
Detection of target molecules is possible by coupling molecular probes to the surface of a bead comprising semi conductor nanoparticles such as cadmium sulfide(CdS) and zinc sulfide (ZnS) nanocrystals. Using the IG-1000 particle size measurement of these nanoparticles was evaluated.
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Measurement of Nano Particles Used in Dye-Sensitized Solar Cell
In this application, the IG-1000 measures the particle size distribution of the porous titanium oxide nano particles which is used in the production of the dye-sensitized solar cell.
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Measurement of Magnetic Nano Particles
The IG-1000 measurement results reflect the state of aggregation of the particles in the liquid (secondary particle size measurement). It is clear from the results that the mean particle size is about 25nm, and that the iron oxide is dispersed in the liquid in the form of nanoparticles.These results suggest the possibility that this type of magnetic particle-dispersed liquid can be used in various applications based on the properties of nanoparticles.
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Measurement of Pigmented Ink Nano Particles
The measured colors consisted of magenta (red),cyan (blue), yellow, and black. Measurement wassuccess fully conducted regardless of the color of the pigmented ink. It is worth mentioning that this type of measurement is difficult to realize in conventional light-scattering based methods. Highly absorbing particles make light difficult to pass through. By overcoming this restriction,the IG-1000 provides a powerful tool for quality control in ink and paint industries.
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Measurement of Nano Particles Used in Fuel Cells
A fuel cell is a kind of battery that continuously produces electric power from supplied oxygen and hydrogen. Water is discharged as a result of the reaction between hydrogen and oxygen. The IG-1000 single nano particle size analyzer is ideal for assessing the dispersion status of nanoparticles in liquids.
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Determination of Tl in Drinking Water using GFA-7000
The AA-7000 in combination with the GFA-7000 is a suitable monitoring system for reliable determination of toxic heavy metals such as thallium in drinking water and mineral water.
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Analysis of Na, K, Ca and Mg in Mineralwater using the Microsampling Method
Sodium, Potassium, Calcium, and Magnesium belong to the essential mineral substances in the human organism. The control of Na, K, Ca and Mg in a variety of mineral waters has been performed according to the actual DIN/ EN regulations [2], with the Shimadzu atomic absorption spectrophotometer AA- 7000 in a fully automatic multi element sequence.
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Measurement of Nano Particles Used in Sunscreen
Ultra fine particles (nanoparticles) made of titanium oxide and zinc oxide, etc. are used in sunscreen to block exposure to ultraviolet rays. However, nanoparticles may easily aggregate to form larger particles which increase the amount of scattered light, resulting in the loss of transparency. Therefore, it is important that the nanoparticles are maintained in a well dispersed state.The IG-1000 single nano particle size analyzer is ideal for assessing the state of nano particle dispersion.
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Measurement of Nano Particles Used in Vehicle Catalytic Converters
The three-way catalyst is a porous body with cerium oxide particles, etc. as the raw material, sintered to the substrate. Making cerium oxide into nano particles increases the active surface area to promote reactivity, and therefore, the particle size distribution within this raw material becomes important.
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GCMS-QP2010 Ultra Application - Analysis of Methyl Laurate Content in Biodiesel Blends and Multidimensional GC/GCMS (Pittcon 2010) (Japan, SSI)
Hops, a major ingredient in beer, contain components called alpha acids (humulones) and beta acids (lupulones). Through the brewing process, alpha acids are converted to iso-alpha acids (isohumulones) which are bitter components of beer. Beta acids are said to influence the bitterness balance. This data sheet shows results of a high-speed analysis of six alpha acids and beta acids.
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Accurate, Reproducible dsDNA Quantitation Using the Small-Volume BioSpec-nano Spectrophotometer
The BioSpec-nano is a state-of-the-art UV-VIS Spectrophotometer for life sciences, ideal for quantitation of nucleic acids or fluorescently-labeled nucleic acids. Completely automated, it offers such features as ‘Drop and Click’ sample mounting-measurement, and cleaning. Manual operations such as moving the optical fiber vertically or cleaning the parts in contact with the liquid are unnecessary. This communication presents data on the accuracy and reproducibility of the BioSpec-nano for dsDNA quantitation.
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UV-Vis Application - UV Talk Letter - (1) Detectors (2) Cell Types and Their Selection V4 C101-E116
In the context of spectrophotometers, the term "detector" refers to a light-receiving element that absorbs the energy of light and consequently induces an electrical change. Types of photoelectric conversion include the external photoelectric effect, a prominent form of which is photoelectric emission from a photoelectric surface into a vacuum, and the internal photoelectric effect, in which photoelectrons are excited into a conduction band. One representative example of a detector based on the former type of photoelectric emission is the photomultiplier tube. Here, of the various aspects of spectrophotometers described in UV Talk Letter Vol. 2, "The Structure of a Spectrophotometer," we will look more closely at these "detectors."
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Blood Vessel Tensile Strength Tests
This report introduces the results of strength tests for a variety of blood vessels (carotid arteries, jugular veins, aortas, inferior vena cava), as well as urinary tracts, and decellularized urinary tracts (urinary tracts from which the cellular components have been chemically removed: in regenerative medicine, this is utilized as artificial blood vessels, with the aim of regeneration through dissemination of one's own cells).
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Measurement of the Compressive Elastic Modulus of Resin Pellets with a Precision Universal Tester Using Trapezium-X
This report describes an attempt to measure the compressive elastic modulus of resin pellets of a variety of shapes and sizes.
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Measurement of Poisson’s Ratio and Elongation for Polyvinyl Chloride (PVC)
In this report, strain is chosen as an example of a physical value showing the properties of typical PVC resin. Strain is measured both in the tensile (vertical) direction and at right angles to the tensile direction (width = horizontal direction) using a noncontact displacement gauge and the Shimadzu precision universal tester, Autograph AG-X. Poisson’s ratio is then obtained using these results.
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Tensile Strength Test for Polychloroprene Rubber
This Application News introduces an evaluation of the most characteristic properties of chloroprene rubber, tensile strength and elasticity.
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Tensile Testing for PET Films
High functional resins including films come in many different varieties, and are involved in a number of research and development projects. Be sure to take advantage of the AG-X for the evaluation of high functional resins.
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Tensile Tests of Various Plastic Materials
Plastics are categorized roughly into “thermoplastic resins” and “thermosetting resins” depending on their process-ability characteristics. This Application News focuses on the former, “thermoplastic resins”, elucidating the features and differences among 4 universally used types of plastics, namely polyethylene (PE), polypropylene (PP), polyvinylchloride (PVC) and polycarbonate (PC), based on their tensile strength properties.
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Tensile Test for Steel Material with High-Accuracy Strain Control
Here we used the Shimadzu Autograph AG-50kNX precision universal testing machine with the SSG-50-10H extensometer (shown in overview of Fig. 1) to perform tensile testing of an SPCC steel sheet sample according to 2 types of strain increase rate control as specified in the proposed ISO 6892 revision, in order to verify the results and load rate control accuracy.
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High Speed Analysis of Alpha Acids and Iso-Alpha Acids in Beer
In the context of spectrophotometers, the term "detector" refers to a light-receiving element that absorbs the energy of light and consequently induces an electrical change. Types of photoelectric conversion include the external photoelectric effect, a prominent form of which is photoelectric emission from a photoelectric surface into a vacuum, and the internal photoelectric effect, in which photoelectrons are excited into a conduction band. One representative example of a detector based on the former type of photoelectric emission is the photomultiplier tube. Here, of the various aspects of spectrophotometers described in UV Talk Letter Vol. 2, "The Structure of a Spectrophotometer," we will look more closely at these "detectors."
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UV-Vis Application - Accurate, Reproducible dsDNA Quantitation Using the Small-Volume BioSpec-nano Spectrophotometer - SSI UV 001
Iso-alpha acids (isohumulones), which give beer its bitter taste, are generated by the isomerization of alpha acids (humulones) contained in hops during the brewing process. Here we present an example of high speed analysis of alpha acids and iso-alpha acids contained in beer.
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AA Application - Measurement of Minerals in Dietary Supplements by Atomic Absorption
Recently, the development and sales of a variety of dietary supplements have increased dramatically against the background of rising public interest regarding health. Here we introduce the method of analysis of minerals in dietary supplements as specified in the Pharmacopoeia of the United States (USP 32), where the supplement market now stands at about three trillion yen (33 billion dollars). As one example, in the case of tablets of oil -and water- soluble vitamins with minerals, the sample preparation and measurement methods are specified for the quantitation of the minerals Ca, Cr, Cu, Fe, K, Mg, Mn, Mo, Se, and Zn, in which flame atomic absorption spectroscopy is used for conducting the quantitation.
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MALDI-Performance Application - Identification of Proteins by LC-MALDI System (1) [Analysis of Samples Obtained from Frozen Tissue Sections] (Japan)
Here, we report on the transmittance measurement of a glass plate coated with TCO film. TCO films are transparent as well as electrically conductive, and examination of the transmittance distribution is important for evaluating both the TCO film and glass substrate. Here we introduce the measurement of the transmittance distribution (map) in glass plate coated with TCO film using the SolidSpec-3700 UV-VIS-NIR spectrophotometer.
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ICP Application - Analysis of Residual Catalysts in Pharmaceuticals Using ICP-AES
The use of LMD permits isolation of a uniform cell cluster from a mixed tissue sample, and offers the added benefit of enabling the analysis of gene- and protein-expression specific to those cells. Despite the difficulty in collecting a large number of cells, it was shown that two-dimensional gel electrophoresis could be conducted from a very small quantity of cells using fluorescent dye (SYPRO Ruby). Furthermore, identification of the phosphorylation site of protein was demonstrated. Here, the sample consisted of oral squamous cell carcinoma isolated from normal mucosal epithelium of human tongue tissue by LMD.
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UV-Vis Application - Transmittance Map of Glass Plate Coated with TCO Film Measured with SolidSpec-3700 Equipped with Automatic X-Y Stage - No. A418
The BioSpec-nano is a state-of-the-art UV-VIS Spectrophotometer for life sciences, ideal for quantitation of nucleic acids or fluorescently-labeled nucleic acids. Completely automated, it offers such features as ‘Drop and Click’ sample mounting-measurement, and cleaning. Manual operations such as moving the optical fiber vertically or cleaning the parts in contact with the liquid are unnecessary. This communication presents data on the accuracy and reproducibility of the BioSpec-nano for dsDNA quantitation.
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MALDI-Performance Application - Identification of Proteins by LC-MALDI System (2) [Analysis of Carbonylated Proteins] (Japan)
It is suggested that selective neuronal cell death occurs in the hippocampus CA1 area due to transient cerebral ischemia, resulting in memory impairment. Here, using a sample consisting of the hippocampus CA1 area affected by transient cerebral ischemia, twodimensional electrophoresis was conducted, and spots thought to be carbonylated proteins were excised and analyzed by LC-MALDI. The results confirmed the carbonylation of the 469th arginine of the Heat shock- 70 kDa protein 1 (Hsp70-1), which plays a role in regulating cell death.
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MALDI-Resonance Application - High-Sensitivity Protein Identification Using AXIMA Resonance (Japan)
Here we present some examples of analyzing saccharides using the reducing sugar analysis system (anion exchange mode) with the Prominence RF- 20AXS.
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MALDI-Resonance Application - Glycopeptide Analysis Using AXIMA Resonance (Japan)
Here we introduce an example of high-sensitivity identification using the μFocus plate together with the AXIMA Resonance.
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HPLC Application - Ion Analysis in Drugs (Part 1) Determination of Counter-ions and Impurity Ions by Ion Chromatography
Here we introduce the simultaneous analysis of 6 related salts of PFOS and PFOA, which differ in their carbon chain length and branches.
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Prominence Application - High Speed with High Resolution Analysis (Part 28) Analysis of α -Acids and β -Acids in Hops
Here, we introduce an example of analysis of ferulic acid in berry juice using a system constructed specifically for efficient handling of this application. Shimadzu’s Prominence UFLC ultra fast LC system forms the base of the system, which also includes a column switching system comprising the first dimension separation column, a trap column, and the second dimension separation column.
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Prominence Application - High Speed with High Resolution Analysis (Part 29) High Efficiency Analysis of Ferulic Acid in Berry Juice Using Automated Column Switching System
Here we introduce the method of analysis of minerals in dietary supplements as specified in the Pharmacopoeia of the United States (USP 32), where the supplement market now stands at about three trillion yen (33 billion dollars).
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HPLC Application - Application of Evaporative Light Scattering Detector (Part 8) Analysis of Terpenoids in Ginkgo Biloba
Here we present examples of analysis of counter-ions and impurity ions at micro levels in drugs using ion chromatography.
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Prominence Application - Applications of the Prominence RF-20AXS Fluorescence Detector (Part 1) High Sensitivity Analysis of Anionic Surfactants, tert-Butylhydroquinone, and Amitrole
Here we introduce examples of high-speed analysis of tocopherols and polycyclic aromatic hydrocarbons using the Prominence RF-20AXS.
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Prominence Application - Applications of the Prominence RF-20AXS Fluorescence Detector (Part 2) High-Speed Analysis of Tocopherols and Polycyclic Aromatic Hydrocarbons
Here we introduce an example of high-speed analysis of α-acids and β-acids in hops using the Prominence UFLCXR ultra fast, high resolution LC system with a high-speed, high-resolution column.
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Prominence Application - Applications of Prominence RF-20AXS Fluorescence Detector (Part 3) Analysis of Saccharides Using Post-Column Derivatization System
The Prominence RF-20AXS fluorescence detector not only achieves high sensitivity levels never before seen (water Raman SN ratio greater than 2000), but it also features a temperature-controlled flowcell with a cooling function as standard to provide highly reliable detection unaffected by ambient temperature fluctuations. Here we introduce examples of high sensitivity analysis using the Prominence RF-20AXS.
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High Speed with High Resolution Analysis (Part 28) Analysis of α -Acids and β -Acids in Hops
Here we present an example of analysis of terpenoids in ginkgo biloba extract using the ELSD-LT II evaporative light scattering detector.
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LCMS-IT-TOF Application - Analysis of Drug Degradants by LC/MS (Japan)
The Axima Performance system for two-dimensional electrophoresis used here is an extremely effective tool for posttranslational modification analysis, as demonstrated in these results of analysis of carbonylated proteins as one type of oxidative stress to proteins.
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LCMS-2020 Application - LC/MS Analysis of Perfluorochemicals (PFCs) Using Impurity Delay Method (Japan)
This Application News introduces an example of highspeed analysis of drug degradants by LC/MS. The ultra fast features of the LCMS-2020, scan rates up to 15,000 u/sec and positive/negative polarity switching as fast as 15 msec, were used in combination with in-source CID (Collision Induced Dissociation) to produce experimental results. In addition, formula prediction was conducted using the LCMS-IT-TOF, and separation of the sample components was carried out using the Prominence UFLCXR.
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GC Application - High Temperature Simulated Distillation using Shimadzu GC-2010
Here we introduce an example of high-speed analysis of α-acids and β-acids in hops using the Prominence UFLCXR ultra fast, high resolution LC system with a high-speed, high-resolution column.
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FTIR Application - FTIR Talk Letter - (1) Using Infrared Detectors (2) Spectral Emissivity Measurement using FTIR Spec V13 C103-E081
To improve productivity, DGC methods have been widely practiced to replace old distillation methods such as D86 and D1160, which are time and labor consuming, and also require complicated operating procedures. In DGC methods, retention times are directly correlated to boiling points and detector responses are correlated to the hydrocarbon concentrations. A calibration curve can be generated by plotting boiling points of n-alkanes as a function of retention times, and sliced peak intensities represent the sample amount distilled. To do so, reproducible retention times and automated software are highly desirable. The GC-2010, equipped with an advanced flow controller, meets the hardware requirements, and Shimadzu distillation GC software for GCsolution is able to achieve automation by integrating calibration, integration, calculation, and report functions.
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Prominence Application - High-Speed Analysis of Terpenoids
Ginger root is not only used as a food ingredient or spice, it is also used as a natural medicine as well. Here we present an example of high-speed analysis of 6-gingerol and 6-shogaol contained in ginger.
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Prominence Applications - High-Speed Analysis of Gingerol and Shogaol
In the previous issue, I presented the TGS (pyroelectric) detector, which is included with Shimadzu FTIR spectrophotometers as a standard feature. Another commonly used infrared detector is the MCT detector. In fact, an MCT detector is incorporated in Shimadzu infrared microscopes as a standard feature, and on FTIR spectrophotometers as an option. Because the MCT detector and TGS detector are based on a different detection principle, there are various differences in the characteristics of the two.
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ICP Application - Role of ICP-AES in Pharmaceutical Fields
Terpenoids, which are contained in large amounts in ginkgo biloba leaf, include bilobalide, ginkgolide A, ginkgolide B, and ginkgolide C. Here we present an example of high-speed analysis of the four components using an evaporative light scattering detector (ELSD).
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Transmittance Map of Glass Plate Coated with TCO Film Measured with SolidSpec-3700 Equipped with Automatic X-Y Stage
A solar cell is composed of various parts, including p-type and n-type semiconductors, anti-reflective film, TCO (transparent conductive oxide) film, and glass plate, etc. Previously, in Application News A409, we introduced an example of measurement of absolute reflectance of anti-reflective film. Here, we report on the transmittance measurement of a glass plate coated with TCO film. TCO films are transparent as well as electrically conductive, and examination of the transmittance distribution is important for evaluating both the TCO film and glass substrate. Here we introduce the measurement of the transmittance distribution (map) in glass plate coated with TCO film using the SolidSpec-3700 UV-VIS-NIR spectrophotometer.
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Identification of Proteins by LC-MALDI System (1) - Analysis of Samples Obtained from Frozen Tissue Sections
We conducted proteome analysis using a two-dimensional gel electrophoresis system and frozen tissue sections obtained by laser microdissection (LMD).Two-dimensional gel electrophoresis is a technique that is recognized for its excellent reproducibility and its ability to quantitate. Therefore, it is still the most widespread analytical method in proteomics.
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Identification of Proteins by LC-MALDI System (2) - Analysis of Carbonylated Proteins
Using the AXIMA MALDI-TOF-MS system for end-point analysis of two-dimensional electrophoresis, we analyzed carbonylated proteins in the CA1 area of a monkey hippocampus affected by ischemia-reperfusion.It is known that reactive oxygen species, or oxygen radicals (ROS), negatively impact a great many diseases, including cancer, cardiac disease and cerebral stroke, which are the leading causes of death among the Japanese, in addition to such lifestyle diseases as diabetes and arteriosclerosis.
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High-Sensitivity Protein Identification Using AXIMA Resonance
The AXIMA Resonance is unique in the world as a mass spectrometer that combines a quadrupole ion trap with a MALDI. The ion trap offers enhanced sensitivity and higher selectivity of precursor ions in MS/MS analysis. Moreover, this effect is further amplified when using the μFocus plate, which enables concentration of the sample on the surface of the MALDI plate.Here we introduce an example of high-sensitivity identification using the μFocus plate together with the AXIMA Resonance.
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Glycopeptide Analysis Using AXIMA Resonance
The AXIMA Resonance is a mass spectrometer that combines Shimadzu’s unique quadrupole ion trap with MALDI. The ion trap is a powerful tool which is suitable for analysis of glycoproteins, complex structures containing sugar chains (glycans) attached to polypeptides through posttranslational modification, because of its ability to conduct MSn measurement (MS/MS, MS3, MS4).
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High Speed with High Resolution Analysis -Analysis of humulones and lupulones in Hops
Hops are one of the basic ingredients in beer and contain -acids (humulones) and β-acids (lupulones). During the brewing process the &Alpha;-acids are converted through isomerization into iso-&Alpha;-acids (isohumulons), which are largely responsible for the bitterness of beer. β-acids, on the other hand, do not produce much bitterness during the boiling stage of beer production, but they are said to play a role in &quot;balancing&quot; the bitter flavor during fermentation and storage. HPLC is generally used for the analysis of &Alpha;-acids and &Beta;-acids, but this analysis typically takes as long as 30 minutes. Here we introduce an example of high-speed analysis of &Alpha;-acids and &Beta;-acids in hops using the Prominence UFLCXR ultra fast, high resolution LC system with a high-speed, high-resolution column.
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High Speed with High Resolution Analysis - High Efficiency Analysis of Ferulic Acid in Berry Juice Using Automated Column Switching System
Here, we introduce an example of analysis of ferulic acid in berry juice using a system constructed specifically for efficient handling of this application. Shimadzu’s Prominence UFLC ultra fast LC system forms the base of the system, which also includes a column switching system comprising the first dimension separation column, a trap column, and the second dimension separation column.
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Application of Evaporative Light Scattering Detector - Analysis of Terpenoids in Ginkgo Biloba
Ginkgo biloba extract contains flavonoids and terpenoids that have been reported to be effective for improving poor blood circulation in the brain as well as poor peripheral blood vessel circulation. This ginkgo biloba extract is used as a health dietary supplement in Japan and the United States. Here we present an example of analysis of terpenoids in ginkgo biloba extract using the ELSD-LT II evaporative light scattering detector.
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Applications of the Prominence RF-20AXS - Fluorescence Detector - High Sensitivity Analysis of Anionic Surfactants, tert-Butylhydroquinone, and Amitrole
The Prominence RF-20AXS fluorescence detector not only achieves high sensitivity levels never before seen (water Raman SN ratio greater than 2000), but it also features a temperature-controlled flowcell with a cooling function as standard to provide highly reliable detection unaffected by ambient temperature fluctuations. Here we introduce examples of high sensitivity analysis using the Prominence RF-20AXS.
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Applications of the Prominence RF-20AXS Fluorescence Detector - High-Speed Analysis of Tocopherols and Polycyclic Aromatic Hydrocarbons
The Prominence RF-20AXS fluorescence detector not only achieves higher sensitivity than ever before possible (water Raman SN ratio greater than 2000), it also includes as a standard feature a temperature controlled cell with a cooling function to provide highly reliable detection irrespective of ambient temperature fluctuations. Moreover, its excellent performance as a fluorescence detector for ultra fast LC is demonstrated with its high-speed sampling and fast response. Here we introduce examples of high-speed analysis of tocopherols and polycyclic aromatic hydrocarbons using the Prominence RF-20AXS.
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Applications of Prominence RF-20AXS Fluorescence Detector - Analysis of Saccharides Using Post-Column Derivatization System
Here we present some examples of analyzing saccharides using the reducing sugar analysis system (anion exchange mode) with the Prominence RF- 20AXS.
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Ion Analysis in Drugs - Determination of Counter-ions and Impurity Ions by Ion Chromatography
Here we present examples of analysis of counter-ions and impurity ions at micro levels in drugs using ion chromatography.
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Analysis Using UV/Py -GC/MS System
Methods used for weather-induced degradation testing of polymeric materials include outdoor exposure methods and weather meters, however, measurement using such methods can take weeks or months to complete. Using a system consisting of a combination of a micro-UV irradiator (UV-1047Xe) and a GC/MS makes it possible to observe degradation products generated due to photo/thermal-oxidative degradation processes in a short period of time. Chemical changes in polymeric materials following these degradation processes can also be determined.
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Measurement of Minerals in Dietary Supplements by Atomic Absorption
Here we introduce the method of analysis of minerals in dietary supplements as specified in the Pharmacopoeia of the United States (USP 32), where the supplement market now stands at about three trillion yen (33 billion dollars).
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Analysis of Food Additives – Food Antioxidants
This Application News introduces the results of analysis of the antioxidants BHA, BHT and TBHQ using a gas chromatograph mass spectrometer (GC/MS). The antioxidants were added to a butter sample, and then extracted using a simple pretreatment process. In this analysis, the backflush technique was employed to prevent high-boiling point substances in the actual sample from being introduced into the detector.
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Universal Testing EZTest Application - Texture analysis of Vermicelli
This Application News introduces the use of EZTest for the texture analysis of salted salmon roe from different regions.
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Universal Testing EZ-X Application - Texture Analysis of Salmon
The Shimadzu EZTest texture analyzer offers dedicated texture-analysis software and a comprehensive range of specialized test jigs for the quantitative evaluation of food texture, including crunchiness, crispiness, and palatability. It is a general-purpose tester that can be used for the strength testing of packaging materials in addition to the development, improvement, and quality control of foods. This Application News introduces the use of EZTest for the texture analysis of salted salmon roe from different regions.
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Prominence Application - High Speed Analysis of Tocopherol
This Application News introduces the tensile test and cutting test of Japanese soumen vermicelli to evaluate the texture. Soumen vermicelli is originated in Nara Prefecture in Japan. It was made by hand by kneading wheat, salt, and water; applying food oil and starch; and then stretching, drying, and maturing. Nowadays, it is generally machine-made. According to JAS (Japan Agricultural Standards) standards, noodles less than 1.3 mm diameter are "soumen," those from 1.3 mm to 1.7 mm diameter are "hiyamugi," and larger noodles are classified as "udon."
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Prominence Application - High Speed Analysis of Ginkgolic Acids
Tocopherol (vitamin E) is not only used as a food additive in antioxidants and nutritional supplements, but it is also a nutrient contained in natural food materials. α-, β-, γ-, and δ- tocopherols are widely known. Here we present an example of high speed analysis of tocopherol.
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Prominence Application - High Speed Analysis of Enrofloxacin
Ginkgo leaves contain ginkgolic acid of alkylphenols that can potentially cause allergies. Here we present an example of high speed analysis using the absorbance of ginkgolic acid and fluorescence detection. Generally, fluorescence detection has the advantages of high selectivity and less likelihood that the actual sample will be influenced by impurities.
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MALDI-Resonance Application - Analysis of O-linked Glycopeptide Using AXIMA Resonance, Multiple Stage Mass Spectrometry - Sensitivity / Analysis (Japan)
Here we introduce an example of analysis of iso-α-acids and α- acids using the ultra-high speed, high resolution Prominence UFLCXR system with a high-speed, highresolution column.
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FTIR Application - Infrared Spectra of Polyvinyl Chloride - No. A421
Here we introduce a pretreatment procedure for removing talc, one type of silicate, from rubber which contains talc. For a description of the procedure for removing calcium carbonate, another additive used in rubber, refer to Application News No. A416.
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FTIR Application - Rubber Analysis by FTIR -Method for Removing Silicate from Rubber- - No. A422
Here we introduce an example of analysis of a gas sample using a single-oven type MDGC. A switching program can easily be created with special software that can be downloaded from the Shimadzu website free of charge.
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GC Application - Analysis of Gas Sample Using Single-Oven MDGC
Detector-switching analysis is different from detector-splitting analysis, so when analysis is conducted using detector switching, the entire sample is flowing into the appropriate detector, and accurate information can be obtained from multiple detectors during a single analysis run without sacrificing sensitivity. (With the detector splitting technique, only a specific fraction of the sample is directed to each of the detectors during the entire analysis.) A switching program can easily be created with special software that can be downloaded from the Shimadzu website free of charge.
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GC Application - Detector-Switching Analysis Using a Capillary Switching Device
Residual organic solvents in food packaging materials is a problem that receives serious attention because of the implications to food safety. GC Application News No. G264 addressed this issue, presenting an example of fast analysis of acrylonitrile in ABS polymer using a backflush GC system. Polystyrene (PS) is widely used in food packaging materials. In this Application News, we introduce an example of fast analysis of residual volatile organic solvents in polystyrene using a backflush GC system.
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GC Application - Fast Analysis of VOCs in Polystyrene Using a Capillary Backflush GC System
Japanese sake typically contains ethyl α-D-glucoside (α-EG), a substance believed to promote beautiful skin, and it is now receiving attention for its effectiveness in improving dry skin conditions even when taken in a drink. Here we present an example of analysis of ethyl α-Dglucoside and other saccharides in sake using hydrophilic interaction liquid chromatography (HILIC).
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HPLC Application - Determination of Chlorhexidine, Benzethonium, and Benzalkonium in Disinfectants
Phthalate is the generic term for the esters combined phthalic acid and various alcohols that are widely used as a plasticizer for various synthetic resin. Here we present the retention behavior and examples of high-speed analysis of phthalates using the ultrahigh speed Prominence UFLC system and various high-speed, high-resolution Shim-pack XR series columns.
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HPLC Application - Analysis of Ethyl a-D-Glucoside in Sake
Here we introduce an example of analysis of gingkolic acids contained in ginkgo biloba leaves.
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Nexera XR Application - High Speed, High Resolution Analysis (Part 30) Analysis of Iso-a-Acids and a-Acids in Beer
Here we introduce examples of these analyses using the Prominence RF-20Axs fluorescence detector with the Prominence Iminoctadine Analysis System and the Prominence Carbamate Analysis System.
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Prominence Application - Applications of the "Prominence RF-20Axs" Fluorescence Detector (Part 4) Analysis of Pesticides with a Postcolumn Derivatization System
Here we introduce analyses of mexiletine, pilsicainide, and zonisamide in serum using the ultra-high speed Prominence UFLC system and high-speed Shim-pack XR-ODS separation column.
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HPLC Application - Analysis of Ginkgolic Acids in Ginkgo Biloba Extract
Here we introduce examples of HPLC analysis of 3 active ingredients (chlorhexidine, benzethonium, and benzalkonium) found in commercially available disinfectants.
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Prominence Application - High Speed, High Resolution Analysis (Part 31) Analysis of Mexiletine, Pilsicainide, and Zonisamide in Serum
Phthalate is the generic term for the esters combined phthalic acid and various alcohols that are widely used as a plasticizer for various synthetic resin. Here we present the retention behavior and examples of high-speed analysis of phthalates using the ultrahigh speed Prominence UFLC system and various high-speed, high-resolution Shim-pack XR series columns.
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Prominence Application - High Speed, High Resolution Analysis (Part 32) Analysis of Phthalates Using Shim-pack XR Series Columns
Here we present an example of measurement of a sodium dodecylbenzenesulfonate solution, in addition to measurement of a sample containing less than 0.050 mg/L TOC using the Shimadzu combustion catalytic oxidation type TOC-VCPH total organic carbon analyzer.
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HPLC Application - High Speed, High Resolution Analysis (Part 32) Analysis of Phthalates Using Shim-Pack XR Series Columns
Here we present an example of qualitative analysis of several types of polymer additives included in commercial food containers and packaging materials using the LCMS-2020.
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LCMS-2020 Application - Analysis of Polymer Additives Using LCMS-2020 (Japan)
Here we present an example of analysis of 8 watersoluble vitamins using a cation exchange-anion exchange multi-mode ODS column, (Scherzo SM-C18, Imtakt Corporation) in conjunction with the LCMS2020 mass spectrometric detector. The gradient consisted of formic acid / ammonium formate buffer and acetonitrile mobile phases, components typical for high sensitivity reverse-phase LCMS analysis.
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LCMS-2020 Application - Analysis of Water-Soluble Vitamins with Multi-Mode ODS Column Using LCMS-2020 (Japan)
Here we present an example of analysis of 8 watersoluble vitamins using a cation exchange-anion exchange multi-mode ODS column, (Scherzo SM-C18, Imtakt Corporation) in conjunction with the LCMS2020 mass spectrometric detector. The gradient consisted of formic acid / ammonium formate buffer and acetonitrile mobile phases, components typical for high sensitivity reverse-phase LCMS analysis.
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LCMS-2020 Application - Ultra High Performance Liquid Chromatography / Mass Spectrometry Using Open Solution Software (Japan)
Here we introduce an evaluation of ion detection sensitivity in analysis of an actual sample consisting of an O-linked glycopeptides, in addition to analysis of the glycosylation site.
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Universal Testing Thermal AGS-X DSC Application - Protein Denaturation and Texture Analysis for Chicken
Here, we looked at the irradiation of sunlight at various angles of incidence on a solar cell, and measured the transmittance of this light irradiated at 6 different angles of incidence (0˚, 10˚, 20˚, 30˚, 40˚, 50˚) using the SolidSpec-3700 UV-VIS-NIR spectrophotometer.
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TOC Application - TOC-V - Recovery Test of Sodium Dodecylbenzenesulfonate using TOC-VCPH
Here we conducted an overall evaluation of texture in fried chicken heated over different time periods. DSC was to measure protein denaturation, and a precision universal testing machine was used to measure the hardness and elasticity.
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UV-Vis Application - Evaluation of Transmittance Dependence on Incident Angle in Glass Plate Coated with TCO Film used for Thin Film Solar Cell - No. A419
Here we conducted measurement of dsDNA at various concentrations at each optical pathlength, and we report the results on the linearity of photometric values (OD) obtained from nucleic acid quantitation, as well as the measurement repeatability as CV (%), and accuracy of the photometric values (OD). In addition, we also investigated the effect of the automatic wiping performance conducted during nucleic acid quantitation.
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UV-Vis Application - Quantitation of Nucleic Acid Using BioSpec-nano - No. A420
Enrofloxacin is a synthetic new quinolone antimicrobial agent. It is used for the treatment and prevention of pneumonia and Escherichia coli diarrhea symptoms in cows and pigs. Here we present an example of high speed analysis of enrofloxacin using fluorescence detection.
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MALDI-Performance Application - A Simple and Highly Successful C-terminal Sequence Analysis of Proteins by Mass Spectrometry (Japan)
many soft PVC samples have shown the similar type of infrared spectrum as the phthalic ester. However, due to concerns about the adverse health affects of these phthalic ester plasticizers, including their carcinogenicity, their application and content levels are regulated. Consequently, soft PVC containing various types of plasticizers instead of those containing phthalic esters are currently being marketed. Since soft PVC containing a large amount of plasticizer displays an infrared spectrum that is greatly affected by the plasticizer, a variety of infrared spectral shapes are seen with soft PVC. Here we introduce and discuss the infrared spectra of this type of soft PVC.
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High Speed Analysis of Ginkgolic Acids
Ginkgo leaves contain ginkgolic acid of alkylphenols that can potentially cause allergies. Here we present an example of high speed analysis using the absorbance of ginkgolic acid and fluorescence detection. Generally, fluorescence detection has the advantages of high selectivity and less likelihood that the actual sample will be influenced by impurities.
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High Speed Analysis of Enrofloxacin
Enrofloxacin is a synthetic new quinolone antimicrobial agent. It is used for the treatment and prevention of pneumonia and Escherichia coli diarrhea symptoms in cows and pigs. Here we present an example of high speed analysis of enrofloxacin using fluorescence detection.
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LCMS-2020 Application - Dual Ion Source DUIS-2020 for Simultaneous ESI and APCI Measurments (Japan)
Here we introduce an example of mass spectrometric analysis of a sample consisting of selectively collected protein C-termini, demonstrating a newly developed, successful method of amino acid sequencing.
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HPLC Application - Improved R & D Efficiency Through Speedier Method Development (2) (Japan)
In LCMS measurement, the ionization mode is selected depending on the polarity of the target compounds. For example, Electrospray Ionization (ESI) is generally selected for analysis of high-polarity compounds which are typically found in drugs and pesticides, while Atmospheric Pressure Chemical Ionization (APCI) is selected for compounds having lower polarity
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Multi-Dimensional Fragmentation of Anthocyanins from Rose Petals by Ion Trap – Time-of-Flight (IT-TOF) Mass Spectrometry
A Shimadzu high performance liquid chromatography – ion trap – time of flight mass spectrometer (LCMS-IT-TOF) was used to aid in the identification of anthocyanin species in rose petals. The effects of varying the parameters which control the collision energy and the collision gas were observed on cyanidin-3,5-diglucoside and its related mono-glucoside and anthocyanidin fragment signals. Further variation in these parameters were used to optimize iterative multi-stage fragmentation (up to MS6 ) in order to elucidate the fragmentation pathway of cyanidin-3,5-diglucoside.
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FTIR Application - FTIR Talk Letter - (1) Integrating Sphere (2) The ABCs of Measurement Methods: KIBR Pellet Method V14 C103-E082
In this report we introduce an example of the analytical method construction process for batch analysis of non-steroidal anti-inflammatory drugs.
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LC/MS Analysis of Perfluorochemicals (PFCs) Using Impurity Delay Method
Using the LCMS-2020 with its excellent sensitivity and repeatability and its newly developed QoQ ion optical system, together with the impurity delay method, allows 0.1 μg/L trace-level quantitative analysis of PFOA and PFOS with excellent repeatability.
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Analysis of Drug Degradants by LC/MS
This Application News introduces an example of highspeed analysis of drug degradants by LC/MS. The ultra fast features of the LCMS-2020, scan rates up to 15,000 u/sec and positive/negative polarity switching as fast as 15 msec, were used in combination with in-source CID (Collision Induced Dissociation) to produce experimental results. In addition, formula prediction was conducted using the LCMS-IT-TOF, and separation of the sample components was carried out using the Prominence UFLCXR.
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Dual Ion Source DUIS-2020 for Simultaneous ESI and APCI Measurement
Since the LCMS-2020 utilizes the standard ESI probe as an integral component, upgrading to the DUIS-2020 is simple and cost efficient. Only a corona needle and secondary high voltage power supply need be added to allow simultaneous and continuous ESI and APCI analysis.
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Improved R&D Efficiency Through Speedier Method Development (1)
Various approaches are available for improving the efficiency of analytical method development. The introduction of high-speed analysis into the method development process for LC and LC/MS is one approach that can directly enhance development efficiency by simply speeding up chromatographic evaluation.
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Improved R&D Efficiency Through Speedier Method Development (2)
An overview of this system was introduced in Technical Report vol. 33 “Improved R&D Efficiency Through Speedier Method Development (1).” In this report we introduce an example of the analytical method construction process for batch analysis of non-steroidal anti-inflammatory drugs.
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Evaluation of a Nanotrap® Biomarker Discovery System
This work will focus on the development and optimization of a biomarker discovery platform combining core shell hydrogel biomarker harvesting, matrix assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry analysis and rapid bioinformatics screening.
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Evaluation of Transmittance Dependence on Incident Angle in Glass Plate Coated with TCO Film used for Thin Film Solar Cell
Here, we looked at the irradiation of sunlight at various angles of incidence on a solar cell, and measured the transmittance of this light irradiated at 6 different angles of incidence (0°, 10°, 20°, 30°, 40°, 50°) using the SolidSpec-3700 UV-VIS-NIR spectrophotometer.
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Quantitation of Nucleic Acid Using BioSpec-nano
We conducted nucleic acid quantitation verification using 1 μL and 2 μL samples of purified dsDNA with pathlengths of 0.2 mm and 0.7 mm, respectively, and obtained excellent results for photometric linearity, measurement repeatability, and accuracy of photometric values.
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Quantitative Determination of Bisphenol A from Human Saliva using MAYI Trap column and LCMS – IT-TOF
The use of bisphenol A (BPA) has been a central topic amongst heated discussions on environmental toxicity. It is present in epoxy resins used in the sealants of canned foods, as a monomer unit of polycarbonate polymers, and as a plasticizer in certain polyvinyl chloride polymers, all of which has led to an increased chance of exposure. Despite a wide body of studies, which report the potential health risks associated with BPA exposure, the actual repercussions are inconclusive. Regardless, a growing need for fast, accurate methods to detect and quantify trace amounts of BPA from biological fluids to assess the validity of arguments from cases presented by both sides of the debate.
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High Speed, High Resolution Analysis (Part 15) Analysis of Ginsenosides in Ginseng
This Application News introduces an example of the analysis of ginsenosides in ginseng using the ultra fast LC system “Prominence UFLC” with the Phenomenex Synergi 2.5 μm Polar-RP high-speed, high-resolution column.
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High Speed, High Resolution Analysis (Part 17) Analysis of Paeoniflorin in Peony Root
This Application News focuses on paeoniflorin as one of the principle active substances in peony root, and introduces an example of analysis of paeoniflorin using the ultra high-speed LC system “Prominence UFLC” with the SPD-M20A photodiode array detector.
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Analysis of Various Vitamins in Multivitamin Tablets
This application news displays the analysis of various vitamins in multivatamin tablets according to the USP proposed techniques for the analysis of dietary supplements.The United States Pharmacopeia (USP) is an official authority that sets standards for all prescription and over–the–counter drugs, dietary supplements and food ingredients that are intended for sale in the United States.
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Analysis of Dietary Supplements
Analytical Instrumentation & Software for the Evaluation, Identification and QA/QC of Dietary Supplements
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Why Is CFT Used to Evaluate Thermosetting Resins?
Resins are generally classified into thermoplastic resins and thermosetting resins. Major examples are polyethylene terephthalate (PET) used in PET bottles and polypropylene (PP) used in kitchen and bath goods.In CFT, viscosity is calculated by measuring the piston's travel distance (travel rate) in the constant test force extrusion method. In this method, even if a thermosetting resin hardens due to heating, only the displacement of the piston stops.
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DUH Dynamic Ultra Micro Hardness Testers are used to Evaluate Mobile phones
As mobile phones evolve, so do their attributes and features. One particular feature is the case of the phone. This article discusses the evaluation of the case of the phone using a dynamic ultra micro hardness tester. The DUH dynamic ultra micro hardness tester has attracted attention due to its ability to measure the size of micro test force indentations based on their depth.
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Multi-Faceted Approach for Evaluating Lithium-Ion Battery Separators
Lithium-ion secondary batteries (in this report, simply referred to as "lithium-ion batteries") are widely used as power sources in cell phones, information devices and other small electronic devices because of their high energy density and high cell voltage. This Application News introduces some examples of physical evaluations of separators, one of the component parts of lithium-ion batteries.
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Oligonucleotide Mass Analysis and In-Source Decay Sequencing on a MALDI TOF Mass Spectrometer
Here we will present a simple and speedy method of sequencing oligonucleotides that requires no further sample preparation and little expertise.
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Particle Size SALD-7101 Application - Evaluation of Small-Angle Forward Scattering Characteristics of Films and Filters
Optical films are used for such purposes as light polarization, anti-reflection and surface protection in liquid crystal and plasma displays (PDP), as well as EL (electroluminescent) displays. However, these films can cause bleeding and blurriness of the displayed image. The state of such image display can be evaluated visually, however, from the standpoint of quality control and improvement of optical film performance, quantitative evaluation is absolutely necessary. The SALD-7101 nano particle size analyzer can be used for this purpose. The forward scattered light within a small-angle region along the optical axis can be detected by the SALD-7101 optical system.
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Particle Size SALD-7101 Application - Evaluation of Small-Angle Forward Scattering Characteristics of Films and Filters #2 Evaluation of Optical Characteristics of 3 Types of Liquid Crystal Protective Film for Cellular Phones
Using the optional SALD-HC71 high concentration sample measurement system with the SALD-7101 nano particle size analyzer, a high concentration sample can be measured without diluting it by inserting it between two closely positioned glass slides. However, with a sample having a concentration of a few hundred ppm, the optical path length will become too short by just inserting the sample between the glass slides, making measurement impossible due to insufficient scattered light intensity.
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Particle Size SALD-7101 Application - Measurement of High Concentration Samples Particle Size Analysis of Pharmaceutical Hand Soap
Here, the size and distribution of micro bubbles generated in both tap water and salt water were measured using the SALD-7101 nano particle size analyzer, and the differences were examined.
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Particle Size SALD-7101 Application - Measurement of High Concentration Samples #2 Measurement of Sports Drink
When particles are dispersed in a liquid, there is a possibility that particle size or particle size distribution can be influenced by particle concentration. Therefore, there is a real need for a way of measuring original source solutions without dilution even when the sample is of high concentration. However, when using a typical flow cell or batch cell for measurement of high concentration samples having particle concentrations (volume concentration) greater than a few hundred ppm, accurate measurement is not possible due to the occurrence of the multiple scattering phenomenon.
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Particle Size IG-1000 Application - Measurement of Carbon Black Nano Particles
Along with the widespread use of high-definition television and digital broadcasting, various new types of displays, including plasma, liquid crystal and organic EL (electroluminescent) displays, have found their way into daily life, and the expectation of consumers is that the images displayed on these devices will be of high quality. Under these circumstances, the optical characteristics, particularly the small-angle forward scattering characteristics along the optical axis of the films and filters used to protect those displays, are very critical to manufacturers due to the adverse affects they may have on the image.
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Particle Size SALD-7101 Application - Size Measurement of Micro Bubbles and Nano Bubbles #3 Comparison of Micro Bubbles Generated in Tap Water and Salt Water
When there are reports of micro bubbles or nano bubbles existing stably over a long period of time, there can be doubt as to whether those are really bubbles or actual particles (suspended solids) instead. However it is difficult to judge whether a particle is a bubble or a solid particle by merely glancing at it. Moreover, even after measuring the bubble size distribution or particle size distribution and the results are examined, it is still difficult to come to a conclusion. However, micro bubbles and particles can be clearly differentiated by comparing their scattered light intensity distribution data.
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Particle Size SALD-7101 Application - Size Measurement of Micro Bubbles and Nano Bubbles #4 Comparison of Scattered Light from Micro Bubbles and Solid (Alumina) Particles
The particle sizes in colloidal silica can be controlled to enable production of colloidal silica with relatively uniform particle sizes. Thus, a variety of applications can be considered for colloidal silica, such as a material in catalysts and coatings. Here we use the SALD-7101 nano particle size analyzer to measure the 3 types of colloidal silica.
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Particle Size SALD-7101 Application - Use of Scattered Light Intensity Distribution Data Colloidal Silica Measurement
An integrating sphere is an optical component used to collect diffused light, average non-uniform light, and for other purposes. This article describes how integrating spheres are used.
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TOC Application - TOC-V - Measuring Samples Per USP 32 Using Shimadzu TOC-VC*H
The United States Pharmacopoeia has established guidelines for determining system suitability and established the acceptance of Water For Injection (WFI) purposes and Purified Water (PW) (USP Revision 32 Method <643>). Shimadzu’s TOC-V*W analyzer is perfectly adapted to meet the requirements of USP Rev. 32 Method <643>. The TOC-V*W analyzer utilizes three forms of oxidation (heat, UV, and persulfate) to provide a low maintenance instrument with a detection limit of 0.0005 mg per liter.
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TOC Application - TOC-VW - Measuring Samples Per USP 32 Using Shimadzu TOC-V*W
Carbon black nano particles are very small and absorb light strongly. Therefore, it has been difficult to measure them with good sensitivity by conventional methods. However, it is now possible to measure carbon black nano particles with good sensitivity using the IG-1000 single nano particle size analyzer, which adopts a new measurement principle, the "induced grating" IG technique.
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Infrared Spectra of Polyvinyl Chloride
Here we introduce and discuss the infrared spectra of this type of soft PVC.
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Determination of Chlorhexidine, Benzethonium, and Benzalkonium in Disinfectants
Here we introduce examples of HPLC analysis of 3 active ingredients (chlorhexidine, benzethonium, and benzalkonium) found in commercially available disinfectants.
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Analysis of Ethyl α-D-Glucoside in Sake
Japanese sake typically contains ethyl α-D-glucoside (α-EG), a substance believed to promote beautiful skin, and it is now receiving attention for its effectiveness in improving dry skin conditions even when taken in a drink. Here we present an example of analysis of ethyl α-Dglucoside and other saccharides in sake using hydrophilic interaction liquid chromatography (HILIC).
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High Speed, High Resolution Analysis (Part 30) Analysis of Iso-α-Acids and α-Acids in Beer
Here we introduce an example of analysis of iso-α-acids and α-acids using the ultra-high speed, high resolution Prominence UFLCXR system with a high-speed, highresolution column.
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Applications of the "Prominence RF-20Axs" Fluorescence Detector (Part 4) Analysis of Pesticides with a Postcolumn Derivatization System
Fluorescence detectors are widely used in the food and environmental fields due to their high sensitivity and selectivity. Among these applications, fluorescence detection is used for analysis of iminoctadine and Nmethyl carbamate pesticides in food and tap water by a post-column fluorescence derivatization method. Here we introduce examples of these analyses using the Prominence RF-20Axs fluorescence detector with the Prominence Iminoctadine Analysis System and the Prominence Carbamate Analysis System.
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Analysis of Ginkgolic Acids in Ginkgo Biloba Extract
Ginkgo leaf extract (ginkgo biloba extract), which contains active ingredients extracted from ginkgo biloba leaves, is reported to be effective in improving cerebral and peripheral blood circulation deficiencies. It is used in Japan and the United States in the form of a dietary supplement, and in Germany, France, and other European countries as a prescription medication. However, alkylphenols which are present in the ginkgolic acids contained in ginkgo leaves are known to cause allergic reactions. For this reason, the United States Pharmacopeia (USP) has established an upper limit for ginkgolic acid content in ginkgo biloba extract. Here we introduce an example of analysis of gingkolic acids contained in ginkgo biloba leaves.
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High Speed, High Resolution Analysis (Part 31) Analysis of Mexiletine, Pilsicainide, and Zonisamide in Serum
HPLC separation and quantitation are often used for measurement of drug concentrations in the blood. When analyzing the concentration of a drug in the blood using HPLC, the separation of contaminants and analytes requires a long analysis time. For that reason, there is demand for faster HPLC analysis to improve the productivity of drug pharmacokinetic studies and clinical testing, particularly when handling many samples. Here we introduce analyses of mexiletine, pilsicainide, and zonisamide in serum using the ultra-high speed Prominence UFLC system and high-speed Shim-pack XR-ODS separation column.
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High Speed, High Resolution Analysis (Part 32) Analysis of Phthalates Using Shim-pack XR Series Columns
Phthalate is the generic term for the esters combined phthalic acid and various alcohols that are widely used as a plasticizer for various synthetic resin. Here we present the retention behavior and examples of high-speed analysis of phthalates using the ultrahigh speed Prominence UFLC system and various high-speed, high-resolution Shim-pack XR series columns.
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Analysis of O-linked Glycopeptide Using AXIMA Resonance™, Multiple Stage Mass Spectrometry
Here we introduce an evaluation of ion detection sensitivity in analysis of an actual sample consisting of an O-linked glycopeptides, in addition to analysis of the glycosylation site.
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A Simple and Highly Successful C-terminal Sequence Analysis of Proteins by Mass Spectrometry
Here we introduce an example of mass spectrometric analysis of a sample consisting of selectively collected protein C-termini, demonstrating a newly developed, successful method of amino acid sequencing.
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Particle Size SALD-7101 Application - High Concentration Sample Measurement (Bad Effect of Multiple Scattering)
From these measurement results, it can be evident that yellow sand from middle east are smaller than that from China, and it can be estimated that particle size of yellow sand may be depending on the flight distance to Japan. Meanwhile, these measurement results include the particle size distribution of cedar pollen. In this way, SALD-7101 may be used for the observation of suspended particulate matters.
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Particle Size SALD-7101 Application - Evaluation of CMP Slurry
Sensitivity and resolution of particle size analysis are depending on both particle size and reciprocal of wavelength. If particle size becomes too small, sensitivity and resolution goes down. Meanwhile, use of short wave length as light source can keep good sensitivity and resolution even in the region of small particles. Therefore, nano particle size analyzer SALD-7101 use the ultra violet laser of 405nm wavelength as a light source. Generally, it is very difficult to evaluate the small particles admixture which is included in the large sample particles. SALD-7101 can improve this difficulty by use of ultra violet laser.
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Particle Size SALD-7101 Application - Relationship between Multiple Scattering & Particle Concentration
In the case of measurement using nano particle size analyzer SALD-7101, suitable optical path length can be selected corresponding to the particle concentration by changing the types of cell. Scattered light sensor for forward area ()Wing sensor Light source Short light path length for High concentration sample Pair of ordinary glass slides Indentation cell (Indemtation depth ; ) Standard light path length(5mm) for the flow cell or batch cell Long light path length for Low concentration sample 500m, 400m, 300m, 200m, 100m and 50m Fig.1 Relationship between optical path length and particle concentration To investigate the relationship between optical path length and particle concentration. Measurement sample is polystyrene latex particles, and its particle concentration of undiluted sample is 10 volume %.
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Particle Size SALD-7101 Application - Relationship between Optical Path Length & Particle Concentration
The United States Pharmacopoeia has established guidelines for determining system suitability and established the acceptance of Water For Injection (WFI) purposes and Purified Water (PW) (USP Revision 32 Method <643>). Shimadzu’s TOC-VC*H analyzer is perfectly adapted to meet the requirements of USP Rev. 32 Method <643>. The TOC-VC*H analyzer utilizes 680°C heat and platinum catalyst to provide a lower maintenance instrument with a detection limit of 0.0040 mg per liter.
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Particle Size SALD-7101 Application - Measurement of Suspended Particulate Matters
If high concentration sample particles are measured by standard method using flow cell or batch cell, multiple scattered light is generated and accurate measurement results cannot be obtained. High concentration smple measurement system using a pair of glass plates can realize accurate measurement of high concentration sample particles.
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Particle Size SALD-7101 Application - Evaluation of Small Difference of Small Admixture
The particle size analysis based on the “Laser diffraction method” utilizes the one to one relations between particle size and spatial scattered light intensity distribution pattern. The multiple scattering breaks this important relations and disturbs accurate particle size analysis. Generally, high concentration sample particles must be diluted before the particle size analysis to prevent bad effect of multiple scattering. However, the dilution of sample may cause the change of particle size or particle size distribution. To investigate the bad effect of the multiple scattering on the particle size analysis, the measurements of milk under the three different measurement conditions were attempted, using SALD-7101 nano particle size analyzer.
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GC Application - Blood Alcohol Analysis using the Shimadzu GC-2010 and AOC-5000 Headspace Autosampler
Milk and lactic acid beverages contain many tiny liquid particles (emulsions) suspended in the liquid. Milk sold in stores is emulsified, so particle size is controlled in the micrometer (μm) range. This process affects the shelf life and flavor of the product. Using laser diffraction particle size analyzer SALD-201V, we tried to investigate the changes in particle size distribution of the milk products of various manufacturers when left to sit for periods of 7 and 9 days.
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Particle Size SALD-201V Application - Changes in Particle Distribution in Milk
Chemical Mechanical Polishing is a process of smoothing surfaces with the combination of chemical and mechanical forces, it can be thought of as a hybrid of chemical etching and free abrasive polishing. CMP process can be used for the production line of LSI such as CPU. In the CMP process, CMP slurry is used and the quality of CMP slurry such as particle size distribution can affect the quality of semiconductor product significantly
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Particle Size SALD-3101 Application - Particle Size Distribution of Loess
Primarily, measurement range of laser diffraction particle size analyzer is determined by specifications of ptical system. Moreover, in the case of wet measurement, the upper limit of measurement range is determined by circulation power of pump. If circulation power of pump is insufficient, large or heavy particles cannot be circulated between flow cell and dispersing bath. The particles which cannot be circulated cannot be measured by detecting scattered light
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Particle Size SALD-3101 Application - Particle Size Distribution of Steel Balls
It is very difficult to measure the loose agglomerate such as ferrite granule in the air. If the injection type dry measurement unit is used. loose agglomerate is dispersed to the primary particles. In this case, SALD-DS3 free fall type dry measurement unit can be used. This type of measurement can be performed by allowing powders to drop freely from the vibrating feeder. Therefore, by using SALD-DS3, fragile sample particles and loose agglomerates can be measured without strong dispersion.
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Particle Size SALD-3101 Application - Comparison of Particle Size Distribution between Star sand and Sun sand
Laser diffraction particle size analyzer SALD-3101 can select three type of sample suction mechnism in the case of dry measurement using SALD-DS21 injection type dry measurement unit.
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Particle Size SALD-3101 Application - Dry measurement using One Shot Sample Suction Method
Loess is homogeneous, porous, friable, pale yellow or buff, typically non-stratified and often calcareous. Loess grains are angular with little polishing or rounding and composed of crystals of quartz, feldspar, mica and other minerals. Loess deposits may become very thick; more than a hundred meters in areas of China and the Midwestern United States. It generally occurs as a blanket deposit that covers areas of hundreds of square kilometers and tens of meters thick.
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Particle Size SALD-3101 Application - Comparison between River Sand and Sea Sand
Two types of fossil foraminifera gathered at beaches of Iriomote island or Taketomi island are known as ”Star sand” and “Sun sand”.Generally, it is very difficult to measure the particle size distribution of these large kinds of sand using laser diffraction particle size analyzer. As the laser diffraction particle size analyzer SALD-3101 has very wide measurement range from 0.05 m to 3000 m and the standard smapler SALD-MS30 has very strong circulation power, SALD-3101 can measure the particle size distribution of Star sand and Sun sand sufficiently.
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Particle Size SALD-7101 Application - Dry Measurement of Ferrite Granule
Driving Under the Influence (DUI) is a serious criminal offense. To prove a suspect guilty often calls for solid scientific evidence. The generally accepted method is to accurately measure the Blood Alcohol Content (BAC). This application presents a static headspace method using Shimadzu GC- 2010 and AOC-5000 headspace autosampler, which offers reliable qualitative analysis, reproducibility and short run times allowing for high throughput.
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Particle Size SALD-2201 Analysis - Particle Size Analysis of Canned Black Coffee
This newsletter presents the results obtained by performing particle size analysis using the SALD-2201 Laser Diffraction Particle Size Analyzer and also describes the relationship between scattered light intensity distributions and sample concentrations.
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Particle Size SALD-2201 Application - Particle Size Analysis of Tea
Here, the SALD-2201 High-Concentration Sample Measurement System was used to analyze a water-in-oil emulsion whose dispersion state is destabilized by dilution. This kind of water-inoil emulsion is often used in the fields of fuels for internal-combustion engines, food products, and chemicals. The analysis of fuel emulsion is presented here.
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Particle Size SALD-2201 Application - Analysis of Particle Size Distribution and Concentration of Rotifers
In the case of the Particle size analysis based on the laser diffraction method, raw data actually detected from optical system is scattered light intensity distribution data. SALD-2201 can be scattered light intensity detection system as well as laser diffraction particle size analyzer.
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Particle Size SALD-2201 Application - Particle Size Analysis of Water-in-Oil Emulsion Using High-Concentration Sample Measurement System
Laser diffraction particle size analyzer SALD-3101 can be applied to various sample particles by using many unique sampling units. The free fall type dry measurement unit SALD-DS3 was developed for SALD-3101 to measure large particles and loose agglomerates in th air.. This type of measurement can be performed by allowing powders to drop freely from the vibrating feeder. Therefore, by using SALD-DS3, fragile sample particles and loose agglomerates can be measured without strong dispersion.
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Particle Size SALD-2201 Application - Particle Size Analysis of of Canned Coffee Using High-Concentration Sample Measurement System
Recently, particle size analysis has come to be used for quality control in the field of food products. In particular, in the field of drink products, an increasing number of products that used to be available only in canned form are now available in plastic bottles, and in consideration of quality, evaluation of the degree of turbidity and size distributions of residual particles is being performed with increasing frequency. This newsletter presents the results obtained by performing the particle size analysis of canned tea and tea in a plastic bottle (same brand). Analysis was performed on the tea in an undiluted form.
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Particle Size SALD-2201 Application - Particle Size Analysis of Magnetized Particles by Dry Measurement
High-Concentration Sample Measurement System can be used to perform the undiluted-state analysis of samples that cannot be diluted or whose dispersion state is changed by dilution, thereby expanding the range of application of the laser diffraction method.
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Particle Size SALD-2201 Application - Particle Size Analysis of Carbon Black Particles
Magnetized particle are used as materials for various product used for various purpose. For Example, controlling particles size distribution of magnetized particles is very important process to develope light but powerful motors. Meanwhile, the particle size analysis of magnetized particles is very difficult, because the dispersion of magnetized particles in liquid is very difficult. We tried particle size analysis of magnetized particles by dry measurement using the special injection nozzle as shown in Fig.1. This nozzle can disperse magnetized particles into air sufficiently by using the shearing force which is generated by changes of cross sections. Therefore, dry measurement can obtain more accurate measurement results comparing with wet measurement.
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Particle Size SALD-2201 Application - Measurement of Two Types of Flour
Carbon black particles are used for cathode as well as anode in the case of lithium ion battery. Control of particle size distribution is very important to develop superior efficiency of lithium ion battery and to keep same efficiency on mass products. Therefor, particle size analyzer must be used to evaluate and keep the properties of carbon black particles in the factories as well as laboratories.
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Particle Size SALD-2201 Application - Measurement of Particle Size Distribution of Two Types of chocolate
Powder milk is one of the most popular sample particles which are used in daily living. We tried to evaluate the difference of particle size distributions before and after the dispersion by means of laser diffraction particle size analyzer SALD-2201. In this measurement, dry measurement unit SALD-DS21 and standard sampler SALD-MS22 were used to try both of dry measurement method and wet measurement method.
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Particle Size SALD-2201 Application - Application of Scattered Light Intensity Distribution Data #1
In the case that distribution width of particle size distribution is not so broad, scattered light intensity data pattern can have first peak as shown in Fig.1. This position of first peak has the one to one relationship with the mean particle diameter of particle size distribution. In other words, the position of first peak is only determined by the mean particle diameter, and its position cannot be changed by effect of refractive index of particle.
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Particle Size SALD-2201 Application - Application of Scattered Light Intensity Distribution Data #2
Only normalized particle size distribution data can be obtained by particle size analysis based on the laser diffraction method, and absolute amount such as volume or number concerning particles cannot be obtained. This method uses only relative change of scattered light intensity pattern depending on the scattering angle to determine the particle size distribution data.
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Particle Size SALD-2201 Application - Application of Scattered Light Intensity Distribution Data #3
In the case that distribution width of sample particles is narrow, the first peak and the first bottom can be found in the scattered light intensity distribution data. In the case that the distribution width is medium, the first peak can be found, but the first bottom is removed. In the case that the distribution width is broad, both of first peak and first bottom can not be found. In this way, the information concerning the distribution width can be obtained using the existence of the first peak and the first bottom.
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Particle Size SALD-2201 Application - Application of Scattered Light Intensity Distribution Data #4
In the case of particle size analysis based on the laser diffraction method, it is very difficult to measure the mixture sample which includes a large quantity of large particles and a small quantity of small particles, because scattered light intensity from small particles is very weak. To investigate this situation, we tried to measure the three kinds of alumina sample particles.
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Particle Size SALD-2201 Application - Application of Scattered Light Intensity Distribution Data #5
Laser diffraction particle size analyzer SALD-2201 can select three type of sample suction mechnism in the case of dry measurement using SALD-DS21 dry measurement unit.
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Particle Size SALD-2201 Application - Dry measurement using One Shot Sample Suction Method
The indentation cell consists of ordinary glass slide and special glass slide with circular indentation as shown in Fig.1, and this cell is used for measurement of medium concentration sample particles together with the high concentration sample measurement system
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Particle Size SALD-2201 Application - Evaluation of Indium Hydroxide Powder
Generally, sample particles must be dispersed by means of various methods, because particle size distribution to be measured is that of primary particles. However, as a special case, loose agglomerate such as suspended materials in the pond water must be measured without dispersion, because particle size distribution of agglomerate is necessary in these kinds of studies.
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Particle Size SALD-2201 Application - Evaluation of Eye Drops Pharmaceuticals
Indium hydroxide is used as material for transparent electrodes and so on. Strict quality control in the production line of indium compound becomes more necessary because of global lack of the indium. Particle size analysis is one of the most important elements for quality control.
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Particle Size SALD-2201 Application - Measurement of Loose Agglomerate
"Such characteristics as "tongue sensation" and "chewability" are elements of the flavor of food. These sensations are affected by particle size distribution of foods, which can be evaluated by laser diffraction particle size analyzer SALD-2201."
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Particle Size SALD-2201 Application - Measurement of Toothpaste Particles
There are several flours which have some properties suitable for respective purpose. Some of properties of flours are determined by particle size distribution. To compare the difference of particle size distributions of two types of flours, laser diffraction particle size analyzer SALD-2201 and injection type dry measurement unit SALD-DS21 can be used. In this measurement, turn table type suction mechanism was used for the suction of sample particles
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Particle Size SALD-2201 Application - Measurement of Powder Milk
Toothpaste is one of the most popular sample particles which are used in daily living. We tried to evaluate the difference of particle size distributions before and after the grinding by means of laser diffraction particle size analyzer SALD-2201. In this measurement, sample particles dispersed in pure water
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Particle Size SALD-3101 Application - Particle Size Analysis of Volcanic Ash from Mount
All particle size analyzers SALD series can measure high concentration sample only by sandwiching the sample particles with pair of glass slides. In this case distance between two glass slide is light path length. Complex control mechanism to keep the fixed light path length and to parallelize it is not necessary, because SALD series use the single laser beam system as shown in Fig.1. In this way, SALD series can realize accurate and stable high concentration sample measurement simply and easily.
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Particle Size SALD-3101 Application - Particle Size Analysis of High-Density Metallic Powders
Recently, even in fields where the sieve method and sedimentation method had previously been used for particle size analysis, there has been an increase in the use of the laser diffraction method due to its large measurement range, short measurement time, good reproducibility, and good operability. Here, the results obtained by performing the particle size analysis of volcanic ash from Mount Usu using the SALD-3101 Laser Diffraction Particle Size Analyzer are presented.
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Particle Size SALD-3101 Application - Dry Measurement of Powdered Detergent
Many units of SALD-3101 laser diffraction particle size analyzer are used for studies of civil engineering, disaster prevention and environmental engineering, because SALD-3101 can measure sand, soil and clay accurately and stably by powerful circulation power of sample particles using combination of SALD-3101 and SALD-MS30 standard sampler. Sample particles used in these studies have very wide distribution width of particle size. SALD-MS30 can circulate the widely distributed sample particles between flow cell and dispersion bath sufficiently without segregation to realize the accurate and stable particle size analysis.
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Particle Size SALD-201V Application - Particle Size Analysis of Canned Coffee
This newsletter presents the results obtained by performing the particle size analysis of four types of canned black coffee using the SALD-2201 Laser Diffraction Particle Size Analyzer.
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Particle Size SALD-3101 Application - Particle Size Analysis of Fine Sand and Clay
In this newsletter, in order to demonstrate the performance of the combination of SALD-3101 and SALD-MS30 in the coarse particle range, an example of the analysis of a metallic powder that has a high specific gravity and consists of particles with large diameters is presented.
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Particle Size SALD-7101 Application - Particle Size Analysis of Liposomes
For these particle size analyses, laser diffraction particle size analyzer SALD-3101 and standard sampler SALD-MS30 were used, which can realize powerful circulation of heavy and large particles.
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Particle Size SALD-7101 Application - Accurate and Stable Measurement of High Concentration Sample by Single Laser Beam System
The results of measuring two types of liposomes using nano particle size analyzerl SALD-7101 are presented here.
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Particle Size SALD-7101H Application - Evaluation of Pigment Ink
SALD-201V is compact model of laser diffraction particle size analyzer, which is suitable for measurements of low density sample particles such as foods and pharmaceuticals
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Ultra High Performance Liquid Chromatography / Mass Spectrometry Using Open Solution Software
The LCMS-2020, with its high scanning speed (up to 15,000 u/sec) and high-speed polarity switching (15 msec polarity switching), demonstrates the performance that satisfies the demands of UHPLC. The powerful user interface of Open Solution makes data review and verification easy, supporting the chemist who wishes to make rapid yet accurate decisions related to his ongoing research.
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Analysis of Water-Soluble Vitamins with Multi-Mode ODS Column Using LCMS-2020
Here we present an example of analysis of 8 watersoluble vitamins using a cation exchange-anion exchange multi-mode ODS column, (Scherzo SM-C18, Imtakt Corporation) in conjunction with the LCMS2020 mass spectrometric detector. The gradient consisted of formic acid / ammonium formate buffer and acetonitrile mobile phases, components typical for high sensitivity reverse-phase LCMS analysis.
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Analysis of Polymer Additives Using LCMS-2020
Here we present an example of qualitative analysis of several types of polymer additives included in commercial food containers and packaging materials using the LCMS-2020.
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LCMS-8030/8040 Application - Achieving Ultra High Speed Analysis with LC/MS/MS (SSI)
High sensitive nano particle size analyzer SALD-7101 H uses high output violet laser (wave length 405nm) and special optical system to realize difficult measurements of the following samples. Low concentration sample of very small particles. Sample particles which can absorb light energy such as carbon black.
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Prominence Application - RF-20A Fluorescence Detector Basics and Applications
This report addresses the problems associated with conventional LC/MS/MS systems in achieving ultra-high-speed analysis, and introduces a resolution to these problems.
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LabSolutions Application - LabSolutions Batch Analysis - Bringing Greater Automation to Analysis Operations
Here we describe the data analysis operations using the browser functions of LabSolutions.
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LabSolutions Application - Using LabSolutions Browser Function for Improved Efficiency & Data Analysis
Here we describe the fundamental principles of the fluorescence detector, and present actual examples of analysis using the Prominence RF-20AXS to illustrate the innovations that provide stable and high sensitivity analysis.
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GC Application - Analysis of Methyl Methacrylate in Polymethyl Methacrylate Plastics by GC
Here, we introduce an example of analysis of electrolytic solvents used in lithium-ion rechargeable batteries by UHPLC.
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HPLC Application - Analysis of Electrolytic Solvents for Lithium-Ion Rechargeable Battery
Here we introduce an example of the analysis of these 3 flavonoids present in ginko bilboa leaves using the SPD-M20A photodiode array detector.
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HPLC Application - Analysis of Hexafluorophosphate Ion in Electrolytic Solution for Lithium-Ion Rechargeable Battery
Here we introduce an example of voglibose tablet testing as specified in the Japanese Pharmacopeia, Fifteenth Edition, suing the Prominence post-column derivatizaiton system with the Prominence RF-20Axs fluorescence detector.
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HPLC Application - Analysis of Flavonoids in Ginkgo Biloba Extract
Here, we introduce an example of analysis of the hexafluorophosphate ion in electrolytic solution used in lithium-ion rechargeable batteries by UHPLC.
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Prominence Application - Applications of the Prominence RF-20Axs Fluorescence Detector (Part 5) Analysis of Voglibose with Postcolumn Derivatization System
JIS K5602 Determination of reflectance of solar radiation by paint film is a test method used for evaluating the solar reflectance of such paint coatings. Here we introduce the measurement of solar reflectance of paint film according to this test method.
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UV-Vis Application - UV and NIR Spectra of Commercial Mineral Water Samples and Quantitation of Mineral Water Sample Mixtures by Multiple Linear Regression - No. A423
Mineral water, consisting of raw water from naturally occurring springs or sources, is now available under many commercial labels. The mineral content in these different brands of water not surprisingly differs depending on their source. Here we investigated these mineral content differences using absorption spectra obtained from measurement of using an ultraviolet, near infrared spectrophotometer. Since the differences in the absorption spectra cannot be seen in the visible region due to the transparency of mineral water, the investigation was conducted in the ultraviolet and near infrared regions. These days, chemometrics is often used for simultaneous quantitation of multiple constituents. An example of the effectiveness of chemometrics quantitation is presented here, in which we applied multiple linear regression to determine the mixture ratios of several commercial mineral water samples.
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UV-Vis Application - Determination of Solar Reflectance of Paint Film in Accordance with JIS K5602 - No. A424
These days, chemometrics is often used for simultaneous quantitation of multiple constituents. An example of the effectiveness of chemometrics quantitation is presented here, in which we applied multiple linear regression to determine the mixture ratios of several commercial mineral water samples
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Nexera Application - The Ultimate in Speed and Resolution
In liquid chromatography acetonitrile is often a mobile phase ingredient of choice because of its elution strength in reverse phase analysis, low viscosity, low absorption in a wide range of the UV spectrum, and relatively low cost. However, in order to improve peak selectivity, researchers sometimes need to look for alternative solvents. Due to a wide pressure range of up to 130 MPa Nexera affords unlimited flexibility in choosing the ideal composition of the mobile phase even utilizing such viscous solvents as methanol-water that significantly increase the back pressure.
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Nexera Application - The UHPLC with the Finest Reproducibility under Ultra-High Speed
Nexera supports the use of long analytical columns thereby maximizing separation efficiency thanks to a high pressure limit of up to 130MPa. In this example, two 150 mm columns with 1.8 µm particle size were connected sequentially thereby increasing Nexera’s theoretical plate number to about 70,000.
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Nexera Application - The UHPLC with the Ultimate in Resolution
The Nexera addresses all analysis requirements : conventional / ultra-high speed / ultra-high resolution / high temperature, with no compromise in data integrity. The wide pressure range increased up to 130 MPa allows Nexera to maximize performance of sub-2-micron particles column. The combination of maximized pressure range and high resolution columns such as sub-2 micro columns realizes genuine ultra high speed and ultra high resolution analysis. The following are the examples of analytical data obtained using the Nexera UHPLC. The data demonstrates the ultrahigh speed and ultra-high resolution that can be obtained with this system.
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Nexera Application - The Lowest Near Zero Carryover
Drawing upon innovations developed by Shimadzu across all industries and applications Nexera achieves superb reproducibility of retention time, separation and the peak area through accurate control of the injection volume, temperature, precise solvent delivery and mixing of solvents. The micro plunger-driven precision solvent delivery control can keep up with the demanding flow rate changes required during the rapid concentration shifts in fast gradient programming. Nexera also offers a ultra-low volume mixer ( 20 μL) based on micro reactor technology. The data demonstrates ultra-fast gradient analysis of alkylphenones.
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Nexera Application - Impurity Analysis by High-sensitivity UV Detector
Nexera autosampler has been designed to provide our customers with the next generation tools for combating sample carryover on an entirely new level. It is now possible to perform a thorough rinse of the sample path and the needle surface by multiple different solvents eliminating the traces even of the stickiest of compounds. This is in addition to the flow-through needle design with minimized contact area which by itself significantly reduces carryover and insures highly reproducible analysis of trace compounds.
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Nexera Application - Use of Methanol Solvent for Separation
Here we introduce an example of analysis of methyl methacrylate in plastics in which polymethyl methacrylate is the main ingredient.
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[ARCHIVED] Supporting Pharmaceutical Cleaning Validation with TOC and UV-Vis Spectrophotometric Analysis
Analysis of impurities can be a challenging task especially when the main ingredient needs to be assayed at the same time, which requires a wider dynamic range and lower baseline disturbances. With an extra wide dynamic range, temperature-controlled flow cell and a stray-light correction function, both SPD-20A UV and SPD-M20A Photo Diode Array detectors offer a high level of stability, sensitivity and linearity for a confident analysis. The 100 Hz sampling of the SPD-20A/M20A ensures peak information can be captured without loss of resolution and exploits the full potential of ultra-fast LC columns.
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Universal Testing EZTest Application - Texture Analysis Multifaceted Measurement of Plums Pickled in Plum Wine
Plum is not only a food but also used as a medicinal foodstuff to recover from fatigue and treat food poisoning. There are many types of plums such as the well-known Gojiro, Shirakaga, Sugita, Akebono, and Nankoh varieties. Yellowish unripened plums are used to make pickled plums and plum wine. Texture and smell of four types of store-bought plums pickled in plum wines were converted into numerical values for comparison.
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Breaking Strain Measurement of ABS Resin
This example describes testing the high-rate tensile strength of a fat plate specimen of ABS resin and measuring strain up to the breakpoint. Images of the high-rate tensile test were captured with a high-speed video camera and image analysis software was used to measure the strain between gauge length on the specimen.
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LCMS-8030/8040 Application - Analysis of Water Soluble Vitamins Using the LCMS-8030 Triple Quadrupole Mass Spectrometer (Japan)
Ultra Fast Speed: Fusion of Ultra Fast MRM and Ultra Fast Polarity Switching An Ultra Fast Scan Speed of 15,000 u/sec 500 MRMs per Second, the Most Ever Possible. High Reliability: Robust System Provides Long-term Data Stability UFsweeperTM Technology Dramatically Minimizes Cross Talk Excellent Linearity with Wide Dynamic Range. User-Friendly: Easy Maintenance for Minimized Instrument Downtime Single-vendor Solution Provides Seamless Operation Quantitation Browser for Effective Multi-analyte Quantitation.
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LCMS-8030/8040 Application - Analysis of Alprazolam, Reserpine and Verapamil Usinf the LCMS-8030 Triple Quadrupole Mass Spectrometer (Japan)
Ultra Fast Speed: Fusion of Ultra Fast MRM and Ultra Fast Polarity Switching An Ultra Fast Scan Speed of 15,000 u/sec 500 MRMs per Second, the Most Ever Possible. High Reliability: Robust System Provides Long-term Data Stability UFsweeperTM Technology Dramatically Minimizes Cross Talk Excellent Linearity with Wide Dynamic Range. User-Friendly: Easy Maintenance for Minimized Instrument Downtime Single-vendor Solution Provides Seamless Operation Quantitation Browser for Effective Multi-analyte Quantitation.
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LCMS-8030/8040 Application - Analysis of Fat Soluble Vitamins Using the LCMS-8030 Triple Quadrupole Mass Spectrometer (Japan)
Ultra Fast Speed: Fusion of Ultra Fast MRM and Ultra Fast Polarity Switching An Ultra Fast Scan Speed of 15,000 u/sec 500 MRMs per Second, the Most Ever Possible. High Reliability: Robust System Provides Long-term Data Stability UFsweeperTM Technology Dramatically Minimizes Cross Talk Excellent Linearity with Wide Dynamic Range. User-Friendly: Easy Maintenance for Minimized Instrument Downtime Single-vendor Solution Provides Seamless Operation Quantitation Browser for Effective Multi-analyte Quantitation.
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LCMS-8030/8040 Application - Structural Analysis of 26 Pharmaceutical Compounds Using Synchronized Survey Scan Measurement (Japan)
Ultra Fast Speed: Fusion of Ultra Fast MRM and Ultra Fast Polarity Switching An Ultra Fast Scan Speed of 15,000 u/sec 500 MRMs per Second, the Most Ever Possible. High Reliability: Robust System Provides Long-term Data Stability UFsweeperTM Technology Dramatically Minimizes Cross Talk Excellent Linearity with Wide Dynamic Range. User-Friendly: Easy Maintenance for Minimized Instrument Downtime Single-vendor Solution Provides Seamless Operation Quantitation Browser for Effective Multi-analyte Quantitation.
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LCMS-8030/8040 Application - Screening of Plastic Additives Using the LCMS-8030 Triple Quadrupole Mass Spectrometer (Japan)
Ultra Fast Speed: Fusion of Ultra Fast MRM and Ultra Fast Polarity Switching An Ultra Fast Scan Speed of 15,000 u/sec 500 MRMs per Second, the Most Ever Possible. High Reliability: Robust System Provides Long-term Data Stability UFsweeperTM Technology Dramatically Minimizes Cross Talk Excellent Linearity with Wide Dynamic Range. User-Friendly: Easy Maintenance for Minimized Instrument Downtime Single-vendor Solution Provides Seamless Operation Quantitation Browser for Effective Multi-analyte Quantitation.
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LCMS-8030/8040 Application - Fast Precusor Ion Scan and Neutral Loss Measurement of Sulfa Drugs Using the LCMS-8030 Triple Quadrupole Mass Spectrometer (Japan)
Ultra Fast Speed: Fusion of Ultra Fast MRM and Ultra Fast Polarity Switching An Ultra Fast Scan Speed of 15,000 u/sec 500 MRMs per Second, the Most Ever Possible. High Reliability: Robust System Provides Long-term Data Stability UFsweeperTM Technology Dramatically Minimizes Cross Talk Excellent Linearity with Wide Dynamic Range. User-Friendly: Easy Maintenance for Minimized Instrument Downtime Single-vendor Solution Provides Seamless Operation Quantitation Browser for Effective Multi-analyte Quantitation.
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Universal Testing EZ-X Application - Texture Analysis of Rice
Recently, numerical conversion of texture of materials and products is becoming more important. Especially in the food industry, various types of evaluation are performed on a wide range of materials, from raw ingredients to product packaging for quality assurance and development of new products. The EZ Test Shimadzu Texture analyzer is used for various purposes such as numerical conversion of texture characteristics of foods such as chewiness, firmness, and palatability; quality evaluation based on the change of hardness; and strength evaluation of food packaging. The analyzer is usable for such a wide range of purposes because a wide variety of jogs can be selectively used according to the type of test being performed. We conducted texture characterization of rice using the Texture Analyzer.
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AA Application - Determination of Cadmium in the Tap Water by Flameless Atomic Absorption Spectrometry
This Application News introduces an example of analysis of epichlorohydrin dissolved from a resin coating on the inside of a can used for canned foods.
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GC Application - Analysis of Epichlorohydrin Dissolved from Metal Food Cans by GC
Here we introduce an example of the separation and qualitative analysis of impurities in the widely used dye, Ru N719, using the LCMS-2020.
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LCMS-2020 Application - Analysis of Impurities of Ru Dye (N719) for Dye-Sensitized Solar Cells( Japan)
Here we introduce examples of impurity analysis of nitrendipine and nifedipine using the Prominence UFLC and LCMS-2020.
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LCMS-2020 Application - Impurity Analysis of Drugs Using Shim-pack XR-Phenyl Column (Japan)
This document describes a method of screening for sulfa drugs using precursor (hereinafter called Prec.) and neutral loss (hereinafter called NL) scans.
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LCMS-2020 Application - Impurity Analysis of Highly-Polar Compounds Without Using Ion-Pair Reagents (Japan)
Here we introduce an example of flameless atomic absorption analysis of tap water spiked with cadmium at 1/10 the concentration specified in the revised ordinance, using the certified river water reference material JAC 0302 (spiked) provided by The Japan Society for Analytical Chemistry. In addition, the convenient automatic dilution and re-analysis function is introduced.
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Precise Lipid Localization from Tissue Sections using a Chemical Inkjet Printer for MALDI Matrix Deposition
Microprinting of matrix solutions combined with imaging using a conventional MALDI TOF-TOF provided accurate mapping of lipids from healthy and diseased tissue sections. This work proved the efficiency of such a methodology when choosing the appropriate printing parameters. Indeed, its automation generated a controlled and reproducible printing process as well as a uniformity of the matrix layer. Both allowed for the efficient detection of gangliosides, PCs and SMs extraction, desorption/ionization and thus detection.
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Nexera Application - Ultra-High-Speed and Ultra-High-Resolution Analysis of Drug Analogs
Red wine contains many types of polyphenols. One of them, resveratrol, is attracting a great deal of attention as an antioxidant that may have the ability to extend life. A high-resolution column is used to resolve the impurity components for the analysis of resveratrol in red wine. A fluorescence detector is effective from both the sensitivity and selectivity viewpoint. This Application Data Sheet introduces the ultra-high-speed analysis of resveratrol in red wine using Nexera with an RF-20Axs high-sensitivity fluorescence detector.
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Nexera Application - Ultra-High-Speed Analysis of Preservatives in Cosmetics
Quinolone antibacterial agents are widely used in livestock, poultry, and farmed fish to prevent and treat infectious diseases. Many types are available. Such simultaneous analysis of multiple components generally requires a long analysis time. However, the combination of a Shim-pack XR Series fast-analysis column and Nexera can significantly reduce the analysis time required. This Application Data Sheet introduces the ultra-high-speed simultaneous analysis of quinolone antibacterial agents using Nexera with an RF-20Axs high-sensitivity fluorescence detector.
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Nexera Application - Ultra-High-Resolution Analysis of Aromatic Carboxylic Acids
Red wine contains many types of polyphenols. One of them, resveratrol, is attracting a great deal of attention as an antioxidant that may have the ability to extend life. A high-resolution column is used to resolve the impurity components for the analysis of resveratrol in red wine. A fluorescence detector is effective from both the sensitivity and selectivity viewpoint. This Application Data Sheet introduces the ultra-high-speed analysis of resveratrol in red wine using Nexera with an RF-20Axs high-sensitivity fluorescence detector.
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Nexera Application - Ultra-High-Speed Analysis of Quinolone Antibacterial Agents
As analogs in drug products often have similar structures, high resolution is demanded for their analysis. A long, sub- 2 μm column is effective in such cases but this requires a UHPLC system with high pressure tolerance, such as the Shimadzu Nexera (130 MPa pressure tolerance). This Application Data Sheet introduces the ultra-high-speed and ultra-high-resolution analysis of ketoprofen and its analogs.
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Nexera Application - Ultra-High-Speed Analysis of Polyphenols in Wine
Parabens and other preservatives are added to cosmetics to maintain safety and quality. The simultaneous analysis of these preservatives generally takes a long time, due to the many types of preservative used. This Application Data Sheet introduces the ultra-high-speed analysis of preservatives in cosmetics using the high resolution of the Shim-pack XR-ODS III column (1.6 µm particle size) and the high pressure tolerance (130 MPa) of the Nexera.
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Ultra-High-Speed Analysis of Polyphenols in Wine (Japan)
Here we introduce analytical conditions that do not include the use of an ion-pair reagent. Using a multi-mode ion-exchange column, a mobile phase consisting only of an aqueous solution of acetic acid and acetate was used. Aminoglycoside antibiotics, compounds that are typically separated by ion-pair chromatography, consists of several sugars and amino sugars. As these compounds display almost no optical absorption, but are easily ionized due to the amino group, a mass spectrometer is the most useful instrument for detection of these compounds.
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Nexera Application - Ultra-High-Resolution Analysis of Polycyclic Aromatic Hydrocarbons
Plants and processed foods contain a variety of hydroxycinnamic acid derivatives and hydroxybenzoic acid derivatives. A high-resolution column is required for their analysis to achieve mutual resolution and resolution from the impurity components in the sample. Methods using a long, sub-2 µm column are available to achieve high resolution, but they demand a UHPLC system with high pressure tolerance. The Nexera 130 MPa pressure tolerance offers adequate capacity to meet these requirements. This Application Data Sheet introduces the ultra-high-resolution analysis of aromatic carboxylic acids using Shimadzu Nexera with a Shim-pack XR-ODS III column.
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Quantitative Analysis of Stable Isotopes of Glucose in Blood Plasma Using Quadrupole GC-MS
In order to investigate the dynamics of glucose in living organisms, a stable isotope of glucose is administered, and its concentration in blood and tissues is measured with quadrupole GC-MS. In this study, we conducted a comparative investigation of the influence of the derivatization method on the results obtained in the quantitative analysis of D-glucose-13C6 using quadrupole GC-MS. As a result, it was discovered that aldonitrile-pentaacetylation gives a single derivatization product, and in mass separation performed with MS, it yielded good quantitative results with little influence by the natural isotopes of glucose that are present at high concentrations in living organisms.
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Application of Metabolomics Techniques using LC/MS and GC/MS Profiling Analysis of Green Tea Leaves
This Application Note presents the profiling of green tea leaves as an example of one application of the metabolome analysis technique using LC/MS and GC/MS. A search of the substances believed to play roles in product quality was conducted, and a quality prediction model was constructed based on the results.
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Achieving Ultra-High-Speed Analysis with LC/MS/MS
This report addresses the problems associated with conventional LC/MS/MS systems in achieving ultra-high-speed analysis, and introduces a resolution to these problems.
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Determination of Cadmium in the Tap Water by Flameless Atomic Absorption Spectrometry
Here we introduce an example of flameless atomic absorption analysis of tap water spiked with cadmium at 1/10 the concentration specified in the revised ordinance, using the certified river water reference material JAC 0302 (spiked) provided by The Japan Society for Analytical Chemistry.
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Determination of Cadmium in Brown Rice by Flame Atomic Absorption Spectrometry
Here we introduce examples of analysis of cadmium in standard reference materials of powdered brown rice (NIES No.10-a,-b) using the typical flame AA method, in addition to a high-sensitivity method using an atom booster.
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RNA Sequence Analysis using the Acid-Hydrolysis Method
The combination of MALDI-TOF-MS and use of the acid-hydrolysis was confirmed to be a powerful technique providing fast and easy base sequencing of RNA sequences of approximately 20 bases.
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Phosphorylation Analysis by MALDI-TOF MS (4)
The extremely stable mass accuracy of the AXIMA Resonance surely contributed to these excellent results. The accuracy obtained in identification of the fragmentation ions was at minimum 16 ppm, very excellent results, indeed. Thus,The combination of the AXIMA Resonance with the μFocus plate provided a high sensitivity platform for successful phosphoprotein identification.
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Phosphorylation Analysis by MALDI-TOF MS (5)
Here we introduce a method for enriching phosphorylated peptides using titanium dioxide (TiO2), as described in "Phosphorylation Analysis by MALDI-TOF MS (3)" using the AXIMA Resonance combined with the μFocus plate.
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Rubber Analysis by FTIR -Method for Removing Silicate from Rubber-
Here we introduce a pretreatment procedure for removing talc, one type of silicate, from rubber which contains talc. For a description of the procedure for removing calcium carbonate, another additive used in rubber, refer to Application News No. A416.
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UV and NIR Spectra of Commercial Mineral Water Samples and Quantitation of Mineral Water Sample Mixtures by Multiple Linear Regression
In this application we demonstrated that multiple regression analysis using absorbance values at multiple wavelengths can be successfully used to perform simultaneous quantitation of multiple sample constituents.
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Determination of Solar Reflectance of Paint Film in Accordance with JIS K5602
JIS K5602 "Determination of reflectance of solar radiation by paint film" is a test method used for evaluating the solar reflectance of such paint coatings. Here we introduce the measurement of solar reflectance of paint film according to this test method.
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Impurity Analysis of Highly-Polar Compounds Without Using Ion-Pair Reagents
Ion-pair reagents which contain fluorine are often used for LC/MS; however, as ion-pair reagents tend to accumulate in the column, interface, switching to another separation mode may take a considerable amount of time to allow complete flushing of the residual ion-pair reagents. Here we introduce analytical conditions that do not include the use of an ion-pair reagent.
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Impurity Analysis of Drugs Using Shim-pack XR-Phenyl Column
Selection of the appropriate column and setting the separation conditions are extremely important in impurity analysis. Although an ODS column is used most of the time, use of phenyl columns is increasing recently with the aim of improving separation. In particular, it is believed that the effectiveness of many pharmaceutical substances is linked to utilization of the aromatic ring π-π interaction. Here we introduce examples of impurity analysis of nitrendipine and nifedipine using the Prominence UFLC and LCMS- 2020.
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Quantitative Analysis of Carbohydrates by LC/MS
Here we introduce examples of quantitative analysis of carbohydrates in foods using post-column solvent addition.
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Analysis of Impurities of Ru Dye (N719) for Dye-Sensitized Solar Cells
Here we introduce an example of the separation and qualitative analysis of impurities in the widely used dye, Ru N719, using the LCMS-2020.
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Analysis of Alprazolam, Reserpine and Verapamil Using the LCMS-8030 Triple Quad Mass Spectrometer
Analysis of Alprazolam, Reserpine and Verapamil Using the LCMS-8030 Triple Quad Mass Spectrometer
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Analysis of water soluble water vitamins using the LCMS-8030 Triple Quad Mass Spectrometer
Analysis of Water Soluble Water Vitamins using the LCMS-8030 Triple Quad Mass Spectrometer. Vitamins include Thiamin, Pyridoxin, Riboflavin, Folic Acid and many more.
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Analysis of fat soluble vitamins using the LCMS-8030 Triple Quad Mass Spectrometer
Analysis of fat soluble vitamins using the LCMS-8030 Triple Quad Mass Spectrometer. Vitamins include: Vitamin A, Vitamin D2, Vitamin D3, Vitamin K1 and many more.
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Structural analysis of 26 pharmaceutical compounds using synchronized survey scan measurement using the LCMS-8030 Triple Quad Mass Spectrometer
Structural Analysis of 26 Pharmaceutical Compounds using synchronized survey scan measurement.
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Screening of plastic additives using the LCMS-8030 Triple Quad Mass Spectrometer
This note describes full scan analysis of a mixture of 12 plastic additives using Synchronized Survey Scan (SSS) as shown in Figure 1. High-speed polarity Switching (15 msec) and rapid scan rates (10000 u/sec) allow multiple collision energies to be employed for automatic product ion scanning .
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Fast precursor ion scan and neutral loss measurement of sulfa drugs using the LCMS-8030 Triple Quad Mass Spectrometer
Fast Precursor Ion Scan and Neutral Loss Measurement of Sulfa Drugs Using the LCMS-8030 Triple Quad Mass Spectrometer.
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Screening of sulfa drugs using the LCMS-8030 Triple Quad Mass Spectrometer
This document describes a method of screening for sulfa drugs using precursor (hereinafter called Prec.) and neutral loss (hereinafter called NL) scans.
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Simultaneous measurement of positive-negative Ions of 30 pesticides for water management using the LCMS-8030 triple quad mass spectrometer.
Japan’s Ministry of Health, Labour and Welfare has designated simultaneous analysis using C18 solid-phase extraction and LC/MS to be the official analytical method for managing targeted pesticides in water (revised on April, 2008). Currently, 31 pesticides are covered. This document describes simultaneous MRM analysis using polarity switching with electrospray ionization (ESI) and LC conditions for 30 of these targeted pesticides (excludes Oxine-Cu). The LCMS-8030 is capable of setting the MRM measurement time of each compound independently. In addition, the polarity switching time is short (15msec), making the LCMS- 8030 suitable for simultaneous analysis of multiple compounds that ionize in both positive and negative modes.
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GCMS-QP2010 Ultra Application - Analysis of Phthalate Esters in Children's Toys Using GCMS (Japan)
As the analysis of polycyclic aromatic hydrocarbons in the environment demands the highly efficient resolution of multiple components, long sub-2 µm columns or multiple columns are often used. Such cases demand a UHPLC system with a high system pressure tolerance. The 130 MPa pressure tolerance of the Shimadzu Nexera can safely and flexibly accommodate such demands. This Application Data Sheet introduces the ultra-high-resolution simultaneous analysis of polycyclic aromatic hydrocarbons using Nexera with two Phenomenex Kinetex columns.
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Analysis of Phthalate Esters in Children's Toys Using GC-MS
As of February 2009, the US Consumer Product Safety Improvement Act, Section 108, mandated that all manufacturers of children’s toys analyze their products for Phthalate Esters. The method to be used is in accordance with the US Consumer Product Safety Commission (CPSC). In this study, six phthalate esters in children's toys were measured using the GCMS-QP2010 Ultra. The simultaneous Scan/SIM data acquisition technique was utilized, with the SIM data used for quantitation. The data showed excellent calibration curve linearity and repeatability. In two of the toy samples, unregulated plasticizers were identified by scan data, illustrating the efficiency of simultaneous Scan/SIM measurement.
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FTIR Application - FTIR Talk Letter - (1) Fourier Transform and Apodization (2) ABC of theFTIR Measurement Methods: Liquid Film Method V15 C103-E083
As of February 2009, the US Consumer Product Safety Improvement Act, Section 108, mandated that all manufacturers of children’s toys analyze their products for Phthalate Esters. The method to be used is in accordance with the US Consumer Product Safety Commission (CPSC). In this study, six phthalate esters in children's toys were measured using the GCMS-QP2010 Ultra. The simultaneous Scan/SIM data acquisition technique was utilized, with the SIM data used for quantitation. The data showed excellent calibration curve linearity and repeatability. In two of the toy samples, unregulated plasticizers were identified by scan data, illustrating the efficiency of simultaneous Scan/SIM measurement.
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Precise Lipid Localization from Tissue Sections using a Chemical Inkjet Printer for MALDI Matrix Deposition
The importance of lipids in cell life is demonstrated by the number of diseases related to their metabolic alteration such as in Alzheimer’s, diabetes, cancer and many other disease states. The most well known roles for lipids in cell function are chemical energy storage and structural components of cell membranes. However, the whole pallet of their function is not yet totally understood partly due to their complexity and the need for new technology and tools for their study.
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Introduction of a New Fast Scanning, Highly Sensitive Single Quadrupole GCMS: A Platform for Modern Chromatography Techniques
A quadrupole GCMS system, capable of scanning 20,000amu/sec and 100Hz, has been developed that meets the data acquisition requirements of the comprehensive GCxGCMSand Fast-GC techniques. In this study, complex natural product extracts will be analyzed that demonstrate the utility of this new GCMS.
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Evaluation of the Current Capabilities of Ultra High Pressure LC and High Temperature LC
UHPLC is becoming an increasingly popular technique for sample analysis due to the ability to reduce analysis times or improve resolution. However, the use of ultrahigh pressures can reduce column lifetime and increase the maintenance requirements of the LC system. Another less commonly used technique to reduce analysis times is the use of high-temperature LC. High-temperature LC has the advantage of reducing system pressure by lowering solvent viscosity and may provide the ability to reduce the organic content of the mobile phase. The use of high-temperature LC has been limited by the number of available columns for this technique. A number of applications will be presented up to 19,000 psi and 150 degrees C for the technique.
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Evaluation of MS Scanning Speeds with UHPLC Peak Widths
The trend toward high-speed LC analysis with reduced column particle sizes continues to increase in popularity. The narrow peak widths of high-speed LC have generally been handled adequately with UV detectors by increasing data acquisition rates. However, use of a MS will reduce the risk of misidentifying peaks when transferring methods from conventional to high-speed LC. Most MS instruments have had difficulty keeping up with these sharp peaks, which may have peak widths of 200 milliseconds or less. Accurate mass analysis of sharp chromatographic peaks obtained by high-speed LC requires ultra-fast MS detection capabilities. A number of high-speed separations will be evaluated using MS scanning speeds of up to 15,000 u/sec with polarity switching speeds of 15 msec.
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UV-Vis Application - UV Talk Letter - (1) Light Sources for Spectrophotometers (2) Measures Solar Transmittance and Solar Reflectance Pt 2 V6 C101-E119
When analyzing a sample by a Fourier transform infrared (FTIR) spectrophotometer, the measurement mode is normally set to transmittance or absorbance. However, unlike a dispersive spectrophotometer, the FTIR instrument does not measure these spectra directly. In FTIR analyses, Infrared light from the light source passes through a Michelson interferometer along the optical path.
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Applications of the Prominence RF-20Axs Fluorescence Detector (Part 5)
Voglibose is a diabetes drug which inhibits the activity of α-glucosidase. HPLC post-column derivatization is specified as the test method for voglibose in the Japanese Pharmacopeia, Fifteenth Edition (voglibose purity test, quantitation of voglibose in pharmaceutical tablets). Here we introduce an example of voglibose tablet testing as specified in the Japanese Pharmacopeia, Fifteenth Edition, using the Prominence post-column derivatization system with the Prominence RF-20Axs fluorescence detector.
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High Speed, High Resolution Analysis (Part 33)
Ginger is not only used as a spice, but has also been used in traditional Chinese herbal medicine since ancient times. Investigation into the efficacy of ginger has been attracting a lot of attention in recent years, and the use of ginger in health foods has seen great increases. Here we introduce an example of the analysis of 6-gingerol and 6-shogaol, constituents of ginger, using the Prominence UFLC ultra fast LC system with the SPD-M20A photodiode array detector.
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Applications Using "Prominence RF-20Axs" Fluorescence Detector (Part 6)
Fluorescence detectors are widely used in the environmental analysis due to their high sensitivity and high selectivity. Even trace amounts of chemical compounds often affect the ecosystem. Bisphenol A is one of such compounds for which trace-level analysis of environmental water is greately required. Here we introduce examples of high-sensitivity analysis of bisphenol A using the Prominence RF- 20Axs fluorescence detector by direct injection and by using an automated column-switching preconcentrating system.
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LCMS-2020 Application - Analysis of Chloramphenicol in Honey Using LCMS-2020 (Japan)
In this issue, we describe the light source, an important part of the spectrophotometer that was explained in “The Structure of a Spectrophotometer” in UV Talk Letter Vol 2”
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MALDI-Performance Application - Analysis of Methylglyoxal-Modified Heat Shock Protein 27 Using LC-MALDI System (Japan)
Here we introduce examples of high-sensitivity analysis of retinol acetate and retinol palmitate, in addition to ultra-high speed analysis of retinol palmitate using the Prominence RF-20Axs fluorescence detector.
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FTIR Application - Quantitative Analysis of Trans Fatty Acid by FTIR - No. A430
This application news introduces a examples of analysis of amines (triethylamine and tributylamine), two of the principle ingredients in polycarbonate plastic.
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GC Application - Analysis of Triethylamine and Tributylamine in Polycarbonate Plastics by GC
Here, we introduce examples of analysis of dimethicone in hair care products using the ELSD-LD II evaporative light scattering detector.
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ICP Application - Fertilizer Analysis by ICPE-9000
Here, we introduce the results of analysis of MG modification of heat shock protein Hsp27 in response to various stresses (acidification, chemical substances, etc.) using the Prominence nano-AccuSpot-AXIMA Performance LC-MALDI system. It is notable that several new modification were found during this experiment.
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Prominence Application - Applications of the Prominence RF-20AXS Fluorescence Detector (Part 7) High Sensitivity Analysis of Retinol Acetate and Retinol Palmitate
Here we used the TGA-51, and instrument capable of continuously measuring water vapor permeability relatively quickly, to measure this property in 5 types of commercially available food wrapping films.
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HPLC Application - Application of Evaporative Light Scattering Detector (Part 9) Analysis of Dimethicone (Dimethylpolysiloxane) in Hair Care Products
Here we introduce an analysis of fertilizer we conducted using the Shimadzu ICPE-9000 Multitype Inductively Coupled Plasma Emission Spectrometer. ICP-AES features a wide dynamic range and high sensitivity, in addition to its applicability to simultaneous multi-element analysis. It is an extremely efficient analytical method, especially when there are many target elements present in a wide concentration range, from the principle constituents to trace level substances.
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Thermal TGA-50 TGA-51 Application - Water Vapor Permeability Analysis of Food Wrapping Film Using TGA-51
Here we introduce the phenol leach test for quantitation of phenol leached from a rubber nipple according to the Food Sanitation Act.
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UV-Vis Application - Leach Test for Phenol in Rubber Nipples - No. A429
This application news introduces an example of analysis of chloramphenicol in honey according to the test method specified in the Japanese official notification. Chloramphenicol is ionized by electrospray ionization (ESI), and is detected as the m/z 321 deprotonated molecule.
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Internal Standard Addition During Pretreatment Using SIL-30AC
Data with excellent repeatability was obtained using both the internal standard "stacked injection" and the internal standard mixing and dilution pretreatment methods. The examples presented here focused on the use of the internal standard addition technique, but in the amino acid analysis example as well, the pretreatment program was utilized to demonstrate that good repeatability can be obtained without concern for even slight differences in reaction time. In particular, the more complicated the pretreatment process, the more difficult it becomes to maintain repeatability when conducting pretreatment manually. For this reason, the Nexera SIL-30AC pretreatment functions were designed to address a wide range of pretreatment processes, from automated primary screening of large numbers of samples to pretreatment requiring accurate reaction time.
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Prominence Application - Applications of the Prominence RF-20AXS Fluorescence Detector (Part 8) High Sensitivity Analysis of Pirimicarb, Tetracycline, Oxytetracycline, o-Phenylphenol, and Diphenyl
Here, we introduce band gap determination from Tauc plots obtained from diffuse reflectance spectra of samples consisting of compound semiconductor materials, which in this case were provided by the Wada Laboratory, Faculty of Science and Technology, Ryukoku University. The experiments were also conducted with the cooperation of the Wada Laboratory, Ryukoku University.
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UV-Vis Application - Measurements of Band Gap in Compound Semiconductors - Band Gap Determination from Diffuse Reflectance Spectra - No A428
Here we introduce a quantitative analysis study we conducted for trans fatty acids by the ATR and transmission methods. Measurement was conducted according to the official method as prescribed by The American Oil Chemists Society (AOCS). Measurement was also conducted by the transmission method in cases where the trans fatty acid content was assumed to be very low.
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The Application of UHPLC to Reduce Sample Analyses Times
The use of UHPLC technology has continued to increase in LC applications as a way to reduce sample analysis turnaround times. Maintaining or increasing resolution of faster separations has been made possible with the use of small particle column technology. UHPLC often provides run times of less than one minute with peak widths of less than 200 milliseconds that have created challenges for system performance and detection methods. A variety of applications in pharmaceutical and environmental areas with small particle columns will be presented that will demonstrate the throughput and performance that can be obtained with current UHPLC technology.
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Reduction of Emissions and Operating Costs of GCMS Usage with a New Ecology Mode
In this work, daily electrical power consumption was measured and compared to that of typical GCMS operation. Measured power correlated to predicted energy savings and emission reductions very closely. A new feature in GCMS software called “Eco-mode” automatically performs the power- and gas-saving functions at the end of an analytical batch. Eco-mode is used to reduce gas flow and turn off unnecessary heated zones, projecting an estimated 35% in energy savings as well as reduction of carbon emissions by up to 1.1 tons per year.
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New Applications of Heart-Cut Multidimensional GC
A new application, improved method UOP960 – trace oxygenated hydrocarbons in liquid hydrocarbon streams by MDGC, has demonstrated the power of twodimensional separation.
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Applications of Comprehensive GCxGCMS Using a Quadrupole Mass Spectrometer With Ultra-high Scan Speed
A new quadrupole GCMS system, capable of scanning 20,000amu/sec and 100Hz has been developed that meets the data acquisition requirements of the comprehensive GCxGCMS technique. In this study, complex naturally-occurring products will be analyzed that demonstrate the utility of this new GCMS.
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EPA Method 8270D Analysis Using Narrow-bore GC Columns and Fast Data Acquisition with a Quadrupole GCMS System
The goal of this study was to demonstrate the capabilities of a new, sensitive, fast-scanning GCMS system to optimize productivity for EPA Method 8270D, while maintaining the strict QC measures of the method, optimizing sensitivity, and expanding the dynamic range. A new, sensitive, fast-scanning quadrupole GCMS system was used to significantly reduce the run time for a mixture of 81 analytes. Excellent sensitivity and chromatographic resolution were demonstrated with a significantly reduced run/cycle time.
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Nexera Application - Ultrafast Analysis of Aldehydes and Ketones
"Various types of synthetic colorants are used as food additives, and gradient elution is generally used to analyze them. Nexera enables stable, ultrafast gradient elution through accurate solution delivery and the use of a high-efficiency gradient mixer. This document introduces an example of ultrafast analysis performed on 12 tar synthetic colorants using Nexera and a Phenomenex Kinetex C18 column (particle size 2.6 µm, a core‐shell column where a 0.35 µm porous membrane is combined with a 1.9 µm solid core)."
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Nexera Application - Ultrafast Analysis of Synthetic Colorants
Water-soluble vitamins such as thiamine (vitamin B1) and riboflavin (vitamin B2) are generally analyzed by reverse-phase ion-pair chromatography. This document introduces an example of ultrafast analysis performed on water-soluble vitamins by gradient elution using Nexera and a Phenomenex Kinetex C18 column (particle size 2.6 µm, a core-shell column where a 0.35 µm porous membrane is combined with a 1.9 µm solid core).
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Nexera Application - Ultrahigh Separation Analysis of Nucleotides
The high sensitivity and selectivity of fluorescence detectors makes them ideal for use in individual test methods such as for pesticide residues in food based on the Japan’s Positive List System. Here we use the Prominence RF-20Axs fluorescence detector to introduce examples of high sensitivity analysis of pirimicarb, tetracycline, oxytetracycline, o-phenylphenol, and diphenyl as individual test method targets based on the Positive List System.
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Nexera Application - Ultrafast Analysis of Water-Soluble Vitamins
"Water-soluble vitamins such as thiamine (vitamin B1) and riboflavin (vitamin B2) are generally analyzed by reverse-phase ion-pair chromatography. This document introduces an example of ultrafast analysis performed on water-soluble vitamins by gradient elution using Nexera and a Phenomenex Kinetex C18 column (particle size 2.6 µm, a core-shell column where a 0.35 µm porous membrane is combined with a 1.9 µm solid core). Aflatoxins are mycotoxins with a carcinogenic property and acute toxicity. They are measured by fluorescence detection HPLC and other instruments in order to prevent contamination of foods. Among four components (B1, B2, G1, G2) quantitated as the total aflatoxin, sensitivity for B1 and G1 is generally enhanced by a derivatization process using trifluoroacetic acid. Using the RF- 20Axs High-Sensitivity Fluorescence Detector enables these components to be directly detected at a high sensitivity without any derivatization process. This document introduces an example of ultrafast, high-sensitivity analysis performed on aflatoxins using Nexera and the RF-20Axs."
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Nexera Application - Ultrafast, Ultra High-Sensitivity Analysis of Aflatoxins
"Nucleotides, building blocks of DNA and RNA, are compounds where phosphate(s) bond to a nucleoside that is composed of a nucleobase and sugar. In general, adenine (A), cytosine (C), guanine (G), thymine (T), and uracil (U) are examples of nucleobases, and ribose and deoxyribose are examples of sugar. There are many other types of nucleotides depending on the number of bonding phosphates (1 to 3).      For high-separation analysis of these components, a long column with a particulate packing material can be used. However, both the UHPLC unit and column need to have a high pressure-resistance. This document introduces an example of ultrahigh separation analysis performed on nucleotides using Nexera, which is resistant to 130 MPa, and a Shim-pack XR-ODS III column (particle size 2.2 µm), resistant to 100 MPa."
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The Ultimate in Speed and Resolution
The Nexera addresses all analysis requirements : conventional / ultra-high speed / ultra-high resolution / high temperature, with no compromise in data integrity. The wide pressure range increased up to 130 MPa allows Nexera to maximize performance of sub-2-micron particles column. The combination of maximized pressure range and high resolution columns such as sub-2 micro columns realizes genuine ultra high speed and ultra high resolution analysis. The following are the examples of analytical data obtained using the NexeraUHPLC. The data demonstrates the ultra-high speed and ultra-high resolution that can be obtained with this system.
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The UHPLC with the Finest Reproducibility under Ultra-High Speed
Drawing upon innovations developed by Shimadzu across all industries and applications Nexera achieves superb reproducibility of retention time, separation and the peak area through accurate control of the injection volume, temperature, precise solvent delivery and mixing of solvents. The micro plunger-driven precision solvent delivery control can keep up with the demanding flow rate changes required during the rapid concentration shifts in fast gradient programming. Nexera also offers a ultra-low volume mixer ( 20 μL) based on micro reactor technology. The data demonstrates ultra-fast gradient analysis of alkylphenones.
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The UHPLC with the Ultimate in Resolution
Nexera supports the use of long analytical columns thereby maximizing separation efficiency thanks to a high pressure limit of up to 130MPa. In this example, two 150 mm columns with 1.8 µm particle size were connected sequentially thereby increasing Nexera’s theoretical plate number to about 70,000.
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Achieving Near Zero Carryover
Nexera autosampler has been designed to provide our customers with the next generation tools for combating sample carryover on an entirely new level. It is now possible to perform a thorough rinse of the sample path and the needle surface by multiple different solvents eliminating the traces even of the stickiest of compounds. This is in addition to the flow-through needle design with minimized contact area which by itself significantly reduces carryover and insures highly reproducible analysis of trace compounds.
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Impurity Analysis by High-sensitivity UV Detector
Analysis of impurities can be a challenging task especially whenthe main ingredient needs to be assayed at the same time, which requires a wider dynamic range and lower baseline disturbances. With an extra wide dynamic range, temperature-controlled flow cell and a stray-light correction function, both SPD-20A UV and SPD-M20A Photo Diode Array detectors offer a high level of stability, sensitivity andlinearity for a confident analysis. The 100 Hz sampling of the SPD-20A/M20A ensures peak information can be captured without loss of resolution and exploits the full potential of ultra-fast LC columns.
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Use of Methanol Solvent for Separation
In liquid chromatography acetonitrile is often a mobile phase ingredient of choice because of its elution strength in reverse phase analysis, low viscosity, low absorption in a wide range of the UV spectrum, and relatively low cost. However, in order to improve peak selectivity, researchers sometimes need to look for alternative solvents. Due to a wide pressure range of up to 130 MPa Nexera affords unlimited flexibility in choosing the ideal composition of the mobile phase even utilizing such viscous solvents as methanol-water that significantly increase the back pressure.
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Ultra-High-Speed and Ultra-High-Resolution Analysis of Drug Analogs
As analogs in drug products often have similar structures, high resolution is demanded for their analysis. A long, sub-2μm column is effective in such cases but this requires a UHPLC system with high pressure tolerance, such as the Shimadzu Nexera (130MPapressure tolerance). This Application Data Sheet introduces the ultra-high-speed and ultra-high-resolution analysis of ketoprofen and its analogs.
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Ultra-High-Speed Analysis of Preservatives in Cosmetics
Parabens and other preservatives are added to cosmetics to maintain safety and quality. The simultaneous analysis of these preservatives generally takes a long time, due to the many types of preservative used. This Application Data Sheet introduces the ultra-high-speed analysis of preservatives in cosmetics using the high resolution of the Shim-pack XR-ODS III column (1.6 µm particle size) and the high pressure tolerance (130 MPa) of the Nexera.
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Ultra-High-Resolution Analysis of Aromatic Carboxylic Acids
Plants and processed foods contain a variety of hydroxycinnamic acid derivatives and hydroxybenzoic acid derivatives. A high-resolution column is required for their analysis to achieve mutual resolution and resolution from the impurity components in the sample. Methods using a long, sub-2 µm column are available to achieve high resolution, but they demand a UHPLC system with high pressure tolerance. The Nexera 130 MPa pressure tolerance offers adequate capacity to meet these requirements. This Application Data Sheet introduces the ultra-high-resolution analysis of aromatic carboxylic acids using Shimadzu Nexera with a Shim-pack XR-ODS III column.
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Ultra-High-Speed Analysis of Quinolone Antibacterial Agents
Quinolone antibacterial agents are widely used in livestock, poultry, andfarmed fish to prevent and treat infectious diseases. Many types are available. Such simultaneous analysis of multiple components generally requires a long analysis time. However, the combination of a Shim-pack XR Series fast-analysis column and Nexera can significantly reduce the analysistime required. This Application Data Sheet introduces the ultra-high-speed simultaneous analysis of quinolone antibacterial agents using Nexera with an RF-20Axs high-sensitivity fluorescence detector.
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Ultra-High-Speed Analysis of Polyphenolsin Wine
Red wine contains many types of polyphenols. One of them, resveratrol, is attracting a great deal of attention as an antioxidant that may have the ability to extend life. A high-resolution column is used to resolve the impurity components for the analysis of resveratrol in red wine. A fluorescence detector is effective from both thesensitivity and selectivity viewpoint. This Application Data Sheet introduces the ultra-high-speed analysis of resveratrolin red wine using Nexera with an RF-20Axs high-sensitivity fluorescence detector.
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Ultra-High-Resolution Analysis of Polycyclic Aromatic Hydrocarbons
As the analysis of polycyclic aromatic hydrocarbons in the environment demands the highly efficient resolution of multiple components, long sub-2 µm columns or multiple columns are often used. Such cases demand a UHPLC system with a high system pressure tolerance. The 130 MPa pressure tolerance of the Shimadzu Nexera can safely and flexibly accommodate such demands. This Application Data Sheet introduces the ultra-high-resolution simultaneous analysis of polycyclic aromatic hydrocarbons using Nexera with two Phenomenex Kinetex columns.
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Ultrafast Analysis of Aldehydes and Ketones
This document introduces an example of ultrafast analysis performed on 13 aldehyde/ketone DNPH derivatives by gradient elution using Nexera and a Phenomenex Kinetex C18 column (particle size 2.6 µm, a core-shell column where a 0.35 µm porous membrane is combined with a 1.9 µm solid core).
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Ultrafast Analysis of Synthetic Colorants
Various types of synthetic colorants are used as food additives, and gradient elution is generally used to analyze them. Nexera enables stable, ultrafast gradient elution through accurate solution delivery and the use of a high-efficiency gradient mixer. This document introduces an example of ultrafast analysis performed on 12 tar synthetic colorants using Nexera and a Phenomenex Kinetex C18 column (particle size 2.6 µm, a core-shell column where a 0.35 µm porous membrane is combined with a 1.9 µm solid core).
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Ultrahigh Separation Analysis of Nucleotides
For high‐separation analysis of these components, a long column with a particulate packing material can be used. However, both the UHPLC unit and column need to have a high pressure‐resistance. This document introduces an example of ultrahigh separation analysis performed on nucleotides using Nexera, which is resistant to 130MPa, and a Shim‐pack XR‐ODS III column (particle size 2.2µm), resistant to 100MPa.
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Ultrafast Analysis of Water-Soluble Vitamins
Water‐soluble vitamins such as thiamine (vitamin B1) and riboflavin (vitamin B2) are generally analyzed by reverse‐phase ion‐pair chromatography. This document introduces an example of ultrafastanalysis performed on water‐soluble vitamins by gradient elution using Nexera and a Phenomenex Kinetex C18 column (particle size 2.6µm, a core‐shell column where a 0.35µm porous membrane is combined with a 1.9µm solid core).
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Ultrafast, Ultrahigh-Sensitivity Analysis of Aflatoxins
Aflatoxins are mycotoxins with a carcinogenic property and acute toxicity. They are measured by fluorescence detection HPLC and other instruments in order to prevent contaminationof foods. Among four components (B1, B2, G1, G2) quantitated as the total aflatoxin, sensitivity for B1and G1is generally enhanced by a derivatization process using trifluoroacetic acid. Using the RF‐20Axs High‐Sensitivity Fluorescence Detector enables these components to be directly detected at a high sensitivity without any derivatization process. This document introduces an example of ultrafast, high‐sensitivity analysis performed on aflatoxins using Nexera and the RF‐20Axs
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ICP Application - ICP Analysis of Herbal Drugs
Herbal drugs are increasingly being used as alternative remedies. Consequently, there is increasing concern regarding their quality and safety. Nherbal drugs are used as pharmaceuticals and food products that contain plants, animals, fungi, minerals, and other natural substances with medical benefits that have not been refined to isolate their active ingredients. Standards regarding the properties, chemical characteristics, and safety of herbal drugs in pharmaceutical form have been officially established by the Japanese Pharmacopoeia and other regulatory institutions. Safety is evaluated by limit testing for heavy metals, arsenic, and other substances. However, since the biological effects of substances such as heavy metals vary for each type of metal, it is necessary to investigate the content of each metal. In this example, I will describe analyzing natural medicines using a Shimadzu ICPE-9000 Multitype ICP Emission Spectrometer. One of the key advantages of this ICP-AES system is its ability to simultaneously measure a large variety of elements with high sensitivity. This means, it provides an extremely effective analytical method for analyzing large numbers of target components present in trace concentrations.
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LCMS-8050 Application - Vitamin D3 Data Presentation for LCMS-8050 (Asia Pacific)
Aldehydes and ketones in the environment can be analyzed using HPLC by derivatizing them with 2,4-dinitrophenylhydrazine (2,4-DNPH). For multicomponent separation of aldehyde/ketone DNPH derivatives, reverse‐phase gradient elution is generally used. Nexera enables stable ultrafast gradient elution through accurate solution delivery and the use of a high-efficiency gradient mixer. This document introduces an example of ultrafast analysis performed on 13 aldehyde/ketone DNPH derivatives by gradient elution using Nexera and a Phenomenex Kinetex C18 column (particle size 2.6 µm, a core-shell column where a 0.35 µm porous membrane is combined with a 1.9 µm solid core).
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Analysis of Methylglyoxal-Modified Heat Shock Protein 27 Using C-MALDI System
In this application we introduce the results of analysis of MG modification of the heat shock protein Hsp27 in response to various stresses (acidification, chemical substances, etc.) using the Prominence nano-AccuSpot-AXIMA® Performance LC-MALDI system. It is notable that several new modifications were found during this experiment.
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Measurements of Band Gap in Compound Semiconductors - Band Gap Determination from Diffuse Reflectance Spectra
In this measurement, the band gap was determined for three types of compound semiconductor materials (CuInSe2, CuIn0.5Ga0.5Se2, CuGaSe2). These are often used as the functional material in solar batteries based on the compound semiconductors , however, as demonstrated here, these 3 types possess different band gap values. Application of the Tauc relational expression, as shown in this example, allows determination of the band gap in powder samples using the diffuse reflectance spectrum
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Leach Test for Phenol in Rubber Nipples
This Application News introduced the phenol leach test for rubber nipples, however, it must be noted that some of the phenol leach conditions are different for types of rubber other than that used in nipples, as well as for metal cans and plastics. In this way, an UV-VIS spectrophotometer can be used for conducting the leach test of phenol from implements, containers, and packaging material used for food.
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Quantitative Analysis of Trans Fatty Acid by FTIR
Here we introduce a quantitative analysis study we conducted for trans fatty acids by the ATR and transmission methods. Measurement was conducted according to the official method<sup>2</sup>) as prescribed by The American Oil Chemists' Society (AOCS). Measurement was also conducted by the transmission method in cases where the trans fatty acid content was assumed to be very low.
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Analysis of Electrolytic Solvents for Lithium-Ion Rechargeable Battery
In this application news we introduce an example of analysis of electrolytic solvents used in lithium-ion rechargeable batteries by HPLC.
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Analysis of Hexafluorophosphate Ionin Electrolytic Solution for Lithium-Ion Rechargeable Battery
In Application News No. L403, we introduced an example of the analysis of electrolytic solvents used in lithium-ion rechargeable batteries. Here, we will investigate the analysis of electrolytes. In order for a substance to be suitable as an electrolyte, it must dissociate in a non-aqueous solvent and be able to withstand the oxidation-reduction environment at the cathode and anode. One such typical electrolyte is lithium hexafluorophosphate. Here, we introduce an example of analysis of the hexafluorophosphate ion in the electrolytic solution used in lithium-ion rechargeable batteries by HPLC.
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Analysis of Flavonoids in Ginkgo Biloba Extract
Ginkgo biloba leaves are said to contain as many as 20 types of flavonoids,and among these are quercetin, kaempferol and isorhamnetin, 3 types that are present in large quantities.Here we introduce an example of the analysis of these 3 favonoids present in ginkgo biloba leaves using the SPD-M20A photodiode array detector.
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High Speed, High Resolution Analysis (Part 33)Analysis of Gingerol and Shogaol in Ginger
Ginger is not only used as a spice, but has also been used in traditional Chinese herbal medicine since ancient times. Investigation into the efficacy of ginger has been attracting a lot of attention in recent years, and the use of ginger in health foods has seen great increases. Here we introduce an example of the analysis of 6-gingerol and 6-shogaol, constituents of ginger, using the Prominence UFLC ultra fast LC system with the SPD-M20A photodiode array detector.
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Applications Using "Prominence RF-20Axs" Fluorescence Detector (Part 6) - Analysis of Bisphenol A at High Sensitivity
Fluorescence detectors are widely used in the environmental analysis due to their high sensitivity and high selectivity. Even trace amounts of chemical compounds often affect the ecosystem. Bisphenol A is one of such compounds for which trace-level analysis of environmental water is greatly required. Here we introduce examples of high-sensitivity analysis of bisphenol A using the Prominence RF-20Axs fluorescence detector by direct injection and by using an automated column-switching pre-concentrating system.
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Applications of the Prominence RF-20AXS Fluorescence Detector (Part 7)High Sensitivity Analysis of Retinol Acetate and Retinol Palmitate
In everyday use, the term "Vitamin A" is synonymous with retinol. Because retinol is a substance that is easily oxidized, highly stable derivatives of retinol are used in such products as foods, medicines and cosmetics. In HPLC analysis of stable retinol derivatives such as retinol acetate and retinol palmitate, a fluorescence detector offers both selectivity as well as high-sensitivity analysis. Here we introduce examples of high-sensitivity analysis of retinol acetate and retinol palmitate, in addition to ultra-high speed analysis of retinol palmitate using the Prominence RF-20AXS fluorescence detector.
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High Sensitivity Analysis of Pirimicarb, Tetracycline, Oxytetracycline, o-Phenylphenol, and Diphenyl
Here we use the Prominence RF-20AXS fluorescence detector to introduce examples of high sensitivity analysis of pirimicarb, tetracycline, oxytetracycline, o-phenylphenol, and diphenyl as individual test method targets based on the Positive List System.
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Application of Evaporative Light Scattering Detector (Part 9)Analysis of Dimethicone (Dimethylpolysiloxane) in Hair Care Products
Dimethicone (dimethylpolysiloxane), a type of silicone oil, is widely used in hair care products such as conditioners and shampoos as an emollient that effectively utilizes the water repellent property of this substance.Here, we introduce examples of analysis of dimethicone in hair care products using the ELSD-LD II evaporative light scattering detector.
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HPLC Application 3rd party - Advantage of a New Generation of Evaporative Light Scattering Detectors: universaally, Higher Sensitivies and Cost-Effectiveness for multi-Element Analyses in Liquid Chromatography- An Application Review (SEDERE)
Among the detectors available in Liquid Chromatography, Evaporative Light-Scattering Detector (ELSD) became in recent years a well established instrument thanks to several theoretical studies based on fundamental investigations and numerous applications provided during the last thirty years. Indeed, ELSD is considered as a nearly Universal, powerful and cost-effective technique, and is ideally appropriate for the majority of the liquid chromatography applications. Today, the power of this detection mode is further extended with a new model which proposes a genuine and efficient Low-Temperature technology (LTELSDTM) combined with an innovative detection chamber, thus providing the highest sensitivities with all compounds including semi-volatile and thermo-labile ones.
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LCMS-IT-TOF Applications - Profiling Analysis of Polysulfide Silane Coupling Agent (Japan)
Among the detectors available in Liquid Chromatography, Evaporative Light-Scattering Detector (ELSD) became in recent years a well established instrument thanks to several theoretical studies based on fundamental investigations and numerous applications provided during the last thirty years. Indeed, ELSD is considered as a nearly Universal, powerful and cost-effective technique, and is ideally appropriate for the majority of the liquid chromatography applications. Today, the power of this detection mode is further extended with a new model which proposes a genuine and efficient Low-Temperature technology (LTELSDTM) combined with an innovative detection chamber, thus providing the highest sensitivities with all compounds including semi-volatile and thermo-labile ones.
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AA Application - Measurement of Cadmium in Brown Rice by Electrothermal Atomic Absorption Method
Machine oil was measured using two different measurement techniques, and a decrese in phenolic antioxidants following oil use was confirmed. In addition, the expectation of an increase in absorption peaks due to C=O stretching vibration and O-H stretching vibration was not observed in these results.
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MALDI-Performance Application - On-Tissue MALDI Analysis: Analysis of Spatiotemporal Changes in Energy Metabolism in a Murine Middle-Cerebral Artery Occlusion Model (Japan)
High-speed analysis of preservatives in cosmetics was previously introduced in Application News No. L375, but we have updated this application as an example of ultra-high-speed analysis with the Nexera LC system in conjunction with the Shim-pack XR-ODS III high-speed separation column.
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FTIR Application - Analysis of Degraded Machine Oil by FTIR - No. A432
Machine oil is used for such purposes as lubrication, cooling, and rust prevention in industries such as automotive, steel, etc. The oil’s quality can become adversely affected when it is used for extended periods of time from contamination and oxidation. Two types of measurement methods, (multiple-reflection ATR and transmission method) were used to conduct measurements of machine oil before and after use to evaluate the degradation due to usage.
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FTIR Application - Hardness DUH-211S Application - Investigation of the Deterioration of UV-Irradiated Resin by FTIR and DUH-211S Dynamic Ultra Micro Hardness Tester - No. A433
This Application News introduces an example of GC-FID analysis of 6 phthalate esters in toys. For an example of GC/MS analysis of the same sample, refer to Shimadzu Application News No. M261 “Analysis of 6 Phthalate Esters in Polyvinyl Chloride Toys by GC/MS.
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GC Application - Analysis of 6 Phthalate Esters in Polyvinyl Chloride Toys by GC-FID
This Applications News introduces an example of GC/MS Analysis of 6 phthalate esters in toys. For an example of GC-FID analysis of the same sample, refer to Shimadzu Application News No G275 “Analysis of 6 Phthalate Esters in Polyvinyl Chloride Toys by GC-FID”.
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GCMS-QP2010 Ultra Application - Analysis of Phthalate Esters in Polyvinyl Chloride Toys by GCMS (Japan)
Here we introduce an example of ultra fast analysis of resveratrol in red wine using the Nexera ultra high performance liquid chromatograph and the Prominence RF-20Axs high-sensitivity fluorescence detector.
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Nexera Application - High Speed, High Resolution Analysis (Part 35) Analysis of Resveratrol in Wine by the Nexera UHPLC System
Recently, in line with the oil-conservation movement, silica, as a non-petroleum resource, is increasingly used as a tire reinforcement filler. Fillers are typically mixed with rubber compounds used in tires to enhance reinforcement of the tire. Because silica has a hydrophilic silanol group on its surface, when added to the lipophilic elastomer rubber, it readily coheres to the rubber without actually bonding to the rubber molecule. Thus, the processibility and mechanical strength of the rubber compound is degraded, greatly affecting tire performance. For this reason, processing with a silane coupling agent is conducted to avoid this shortcoming.
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Nexera Application - High Speed, High Resolution Analysis (Part 34) Analysis of Preservatives in Cosmetics by the Nexera UHPLC System
Here we introduce an example of ultra fast analysis of resveratrol in red wine using the Nexera ultra high performance liquid chromatograph and the Prominence RF-20Axs high-sensitivity fluorescence detector.
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LCMS-8030/8040 Application - Analysis of Pesticides Using the Triple Quadrupole LC/MS/MS 8030 (Japan)
Antioxidants, ultraviolet absorbers, flame retardants and other polymer additives are indispensable for improving the characteristics and functionality of polymers. Understanding the types and quantities of these additives to be used is extremely important to effectively control the manufacturing process of the polymers. Here is an example of quantitative analysis of 14 types of polymer additives using MRM (Multiple Reaction Monitoring) and the LCMS-8030 triple quadrupole mass spectrometer.
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LCMS-8030/8040 Application - Analysis of Polymer Additives Using the LCMS-8030 Triple Quadrupole LC/MS/MS (Japan)
Here, using the Shimadzu MALDI imaging system comprised of the CHIP-1000 and AXIMA Performance to elucidate the distribution of regiospecific energy metabolism-related substances, we introduce our latest reported research results in which the ischemic region reported research results in which the ischemic region and its periphery (ischemic penumbtra) are clearly distinguished.
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Nexera Application - High Speed, High Resolution Analysis (Part 36) Analysis of Quinolones by the Nexera UHPLC System
Here we present an example of analysis of cadmium in a standard sample (NIES No. 10a) of brown rice by the high-sensitivity electrothermal atomic absorption method using the AA-7000.
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TOC Application - TOC-V - Measurement of Oil Residues on Copper Wire Surface by TOC Solid Sample Measurement System
Here, we introduce an example analysis of a mixture of synthetic quinolone antibacterials using the Nexera ultra high performance LC system with the RF-20Axs high-sensitivity fluorescence detector.
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HPLC Application 3rd party - Assessment of a New Generation of Evaporative Light Scattering Detectors for Liquid Chromatography: Sensitivity, Linearity, Dynamic Range, Analyte Dispersion and Response Variation with Eluent Compositions (SEDERE)
Here, we introduce an example of measurement of copper wire using the solid sample measurement system comprising the TOC-V CPH total organic carbon analyzer and the SSM-5000A solid sample combustion unit.
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GCMS Application - GCMS Forensic Toxicological Database, Part 1 (Japan)
In this work, several examples and data are provided on these topics to emphasize the great advancement in this technology, thus bringing to the analyst a relevant and cost-effective solution to their chromatography challenges.
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AA Application - AA Analysis of Chromium in Gelatin
We analyzed the diluted gelatin for food and the diluted gelatin with chromium, cadmium and lead by ICP-AES. Each gelatin was diluted 100 times with distilled water. Acetic acid and a surface active surfactant were added to prevent solidification of the solution. The concentration of added each element was chromium 1mg/kg (0.01mg/L in the solution), cadmium 0.2mg/kg (0.002mg/L in the solution) and lead 2mg/kg (0.02mg/L in the solution). Chinese standard of the chromium concentration is 2mg/kg.
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ICP Application - ICP Analysis of Hazardous Elements in Gelatin
This Application News introduces the usefulness of the forensic substance library in this GC/MS Forensic Toxicological Database.
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GCMS-QP2010 SE Application- USEPA Method 8260 Using EST Purge-and-Trap and GCMS-QP2010S (EST)
We analyzed the diluted gelatin for food and the diluted gelatin with chromium by furnace method. Each gelatin was diluted 500 times with distilled water. The concentration of added chromium was 4 ppb considering Chinese standard (2mg/kg).
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Texture Analysis of "Soumen" Japanese Vermicelli
The evaluation of the mechanical properties of foods, such as strength and hardness, is becoming widely used for the numerical comparison and control of food texture. This Application News introduces the tensile test and cutting test of Japanese soumen vermicelli to evaluate the texture.
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Compression Test for Structural Materials of Lithium-Ion Batteries
Since lithium-ion batteries are light and small, they are used in a wide variety of products, from mobile electronic devices such as cellular phones and notebook PCs to electric cars and hybrid cars. Their inner structural materials are subjected to external force during production processes and to pressure during use. Therefore, evaluation of strength of each structural material is important to maintain consistent quality. A strength measurement was performed on thin or minute materials among various structural materials of lithium-ion batteries. Separators are usually evaluated by a tensile test or penetration test. A compression test is also important to evaluate them because they are compressed in some processes. Active materials of approximately 10 μm in size located near the electrode need to have a certain compression strength so they will not be destroyed during the coating process. Below are the results of compression tests performed on these materials using the MCT-211 Series Micro Compression Testing Machine.
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Evaluation of Kinetex Column Peak Capacity Using the Shimadzu Nexera UHPLC System
Peak capacities of 200 could be obtained with a 24 second gradient runtime for the compounds that were tested. All compounds were eluted from the column in less than 15 seconds. These results demonstrate the combined benefits of high column efficiency and system performance.
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Measurement of Texture of Pork by a Penetrating Strength Test
The EZ Test Shimadzu Table-Top Universal Tester can be used to convert texture-related properties such as crispness of croquettes, resilience of bread, and firmness of boiled fish paste (kamoboko) as well as tenderness of pork reported in this document.
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Evaluation of Compression Strength of Micro Particles Contained in Scrubs Using the MCT-W500 Micro Compression Testing Machine
It was found that depending on the type of micro particle used in the scrubs, there were large differences in shape and size, as well as in compression strengths (reference strength) and fracture pattern. By accumulating these data it is hoped that they will be effective indicators for designing particles for facial cleansers.
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Multifaceted Measurement of Plums Pickled in Plum Wine
In the test, pickled plums were evaluated by a mechanical test, image observation. Although the results were sufficiently convincing, multifaceted analysis and measurement are believed to help convert the texture of food into numerical values.
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Measurement of Texture Characteristics of Rice Using the EZ Test Shimadzu Texture Analyzer
Koshihikari showed over twice as high values as compared to the other two types of rice for all items; hardness, cohesiveness, and cohesion strength. Cohesiveness is believed to closely indicate the sensation people fell when they actually eat rice. The test result for cohesiveness clearly indicates Koshihikari's characteristics. The use of the EZ Test Texture Analyzer is recommended for evaluating food texture because it provides easier texture characterization as compared to sensory evaluations and is highly operable.
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Hardness Test on Extra Fine Wire by Shimadzu DUH-W201S Dynamic Ultra Micro Hardness Tester
The Shimadzu DUH-W Dynamic Ultra Micro Hardness Tester allows specimen hardness to be simply evaluated without embedding the specimen in resin.
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Hardness Test on Propelling Pencil Lead Core by Shimadzu DUH-W201S Dynamic Ultra Micro Hardness Tester
Propelling (or mechanical) pencil lead cores are made by mixing black lead with polymer resin. The hardness symbols HB and B are evaluated by a density measurement tester according to the density obtained by drawing on line drawing paper; however, hardness is not yet scientifically defineThe following introduces an example of a hardness test performed on propelling pencil lead cores using the Shimadzu Dynamic Ultra Micro Hardness Tester.
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Texture Evaluation of Cheese and Agar Jelly
Texture evaluation is a way to convert physical senses (texture) into numerical values. Properties can be calculated as hardness, brittleness, cohesion strength, cohesiveness, elastic quality, cohesive quality, and adhesion as representative values. The section below introduces the results of a mastication test and shearing test conducted on cheese and agar jelly using the EZ Test Shimadzu Compact Table-Top Testing Machine as a texture evaluation example.
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Compression Testing and Observation of Deformation of Bonding Wire Au Balls by Shimadzu MCT-W500 Micro Compression Testing Machine
As introduced in this example, mounting the side observation kit on the Shimadzu MCT-W500 Micro Compression Testing Machine allows the compression test behavior of micro specimens to be investigated from both numerical values (graph, raw data) and images, which enables more reliable results in line with changes in the test subject to be obtained.
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Compression Test on Brake Friction Materials by Shimadzu MCT-W500 Micro Compression Testing Machine
Friction brakes mounted on automobiles, for example, are indispensable for ensuring safety.Friction brakes operate by using the friction between two solid objects to create braking action. R&D, for example, into modifcation of raw materials is being conducted to improve braking performance. As an evaluation method for viewing the properties of brake friction materials, the following introduces an example of a compression test performed using a fat indenter to measure the relationship between force and displacement.
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Hardness Test on Hair by DUH-211S Ultra Micro Hardness Tester
In this test, we tried evaluating hair by "hardness" that had deteriorated, in particular, as a result of being treated with chemicals, and hair, on the other hand, that was not treated by any artificial means. The "Shimadzu DUH-211S Ultra Micro Hardness Tester", which is compatible with the measurement of micro objects, was used for measuring hardness.
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Texture Analysis of "Ikura" Salted Salmon Roe by EZTest
This Application News introduces the use of EZTest for the texture analysis of salted salmon roe from different regions. The Shimadzu EZTest texture analyzer offers dedicated texture-analysis software and a comprehensive range of specialized test jigs for the quantitative evaluation of food texture, including crunchiness, crispiness, and palatability. It is a general-purpose tester that can be used for the strength testing of packaging materials in addition to the development, improvement, and quality control of foods.
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Compression Test on Ribs of PDP Rear Panelby Micro Compression Testing Machine
The following introduces an example of a compression test performed on a typical rib specimen on the PDP rear panel using the "Shimadzu MCT-W500 Micro Compression Testing Machine" to measure the relationship between breaking strength and force/displacement.
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Hardness Test on Vickers Hardness Standard Blocksby DUH-211S Dynamic Ultra Micro Hardness Tester
The Shimadzu DUH-211 Dynamic Ultra Micro Hardness Tester can test thin-flm samples such as these and has a function for converting to Vickers hardness according to ISO. The following introduces the result of a hardness test performed on Vickers hardness standard blocks to check Vickers hardness conversion accuracy on this system.
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Damage Evaluation for Cutting Tool (Tip) Edgeby Micro Compression Testing Machine
As a proposed means for evaluating strength, a compression test was performed in the vicinity of the edge to measure the relationship between breaking strength and test force/displacement. The following introduces an example of this.
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Compression Test on Hollow Beaded Glassby Micro Compression Testing Machine
The following application news introduces an example of a compression test performed on hollow glass beads using the "MCT-W500 Micro Compression Testing Machine"
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UV-Vis Application - UV Talk Letter - (1) BioSpec-nano Life Science UV-Vis Spectrophotometer (2) Nucleic Acid Thermal Stability Analysis (Tm Analysis ) V7 C101-E120
The USEPA developed Method 8260B for the determination of Volatile Organic Compounds (VOCs) in an array of waste matrices. Over the years, there have been many advances in purge and trap sampling and GCMS analysis. There advances have aided in carryover reduction, water management and reduced method detection levels. This paper will present the optimum purge and trap and GCMS parameters used to generate both soil and water USEPA Method 8260B data.
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Measurement of Oil Residues on Copper Wire Surface by TOC Solid Sample Measurement System
In this application, we introduce an example of measurement of copper wire using the solid sample measurement system comprising the TOC-VCPH total organic carbon analyzer and the SSM-5000A solid sample combustion unit.
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Measurement of Cadmium in Brown Rice by Electrothermal Atomic Absorption Method
Here we present an example of analysis of cadmium in a standard sample (NIES No. 10-a) of brown rice by the high-sensitivity electrothermal atomic absorption method using the AA-7000.
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Analysis of Degraded Machine Oil by FTIR
Two types of measurement methods, (multiple-reflection ATR and transmission method) were used to conduct measurements of machine oil before and after use to evaluate the degradation due to usage.
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Investigation of the Deterioration of UV-Irradiated Resin by FTIR and DUH-211S Dynamic Ultra Micro Hardness Tester
Resins may exhibit deterioration by any number of ways including heating, exposure to various organic solvents, acidic or alkaline solutions, and ultraviolet irradiation. Therefore, a high tolerance to ultraviolet irradiation is especially necessary for resin products used outdoors. Introduced here is an investigation in which various types of plastics were irradiated with ultraviolet light. Afterward, the samples were analyzed for chemical and physical changes caused by ultraviolet irradiation using FTIR and DUH-211S Dynamic Ultra Micro Hardness Tester.
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On-Tissue MALDI Analysis: Analysis of Spatiotemporal Changes in Energy Metabolism in a Murine Middle-Cerebral Artery Occlusion Model
Here, using the Shimadzu MALDI imaging system comprised of the CHIP-1000 and AXIMA Performance® to elucidate the distribution of regiospecific energy metabolism-related substances, we introduce our latest reported research results in which the ischemic region and its periphery (ischemic penumbra) are clearly distinguished.
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FTIR Application - FTIR Talk Letter - (1) Gas Cell (2) Measurement Methods ABCs: Diffuse Reflectance Method V16 C103-E084
Life science research involves the handling of extracted and purified nucleic acids (DNA, RNA) in conducting PCR (Polymerase Chain Reaction), quantitative PCR, cloning, base sequence determination, DNA micro-array analysis, etc. Nucleic acid analysis using ultraviolet-visible spectrophotometers is widely conducted to successfully guide these processing operations. This document introduces the BioSpec-nano UV-VIS spectrophotometer, which provides simple and quick analysis of micro-level nucleic acids.
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Nexera Application - High Speed, High Resolution Analysis (Part 37) Analysis of Clobazam and Cibenzoline in Serum by the Nexera UHPLC System
Samples of various states can be analyzed by FTIR by selecting the appropriate accessory attachment. Here we introduce the structures and method for selecting a gas cell used for measurement of gas samples.
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High Speed, High Resolution Analysis (Part 34) Analysis of Preservatives in Cosmetics by the Nexera UHPLC System
High-speed analysis of preservatives in cosmetics was previously introduced in Application News No. L375, but we have updated this application as an example of ultra-high-speed analysis with the Nexera LC system in conjunction with the Shim-pack XR-ODS III high-speed separation column (particle size 1.6 μm).
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High Speed, High Resolution Analysis (Part 35) Analysis of Resveratrol in Wine by the Nexera UHPLC System
Resveratrol, a type of polyphenol, is a phytoalexin that is synthesized by plants when they are exposed to stress due to disease and pests. In addition to providing antioxidative effects, resveratrol is reported to offer other health benefits including support for increased longevity. For these reasons, this substance is actively being researched, and recently, resveratrol is being added to food products and to cosmetics. Here we introduce an example of ultra fast analysis of resveratrol in red wine using the Nexera ultra high performance liquid chromatograph and the Prominence RF-20Axs high-sensitivity fluorescence detector.
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High Speed, High Resolution Analysis (Part 36) Analysis of Quinolones by the Nexera UHPLC System
Many types of quinolone synthetic antibacterials are widely used for the prevention and treatment of infectious diseases in domestic animals, domestic fowl, and cultured fish. Here, we introduce an example of simultaneous analysis of a mixture of synthetic quinolone antibacterials using the Nexera ultra high performance LC system with the RF-20Axs high-sensitivity fluorescence detector.
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Analysis of Halogens in Polyphenylene Sulfide Resin Using a Combustion-Ion Chromatography System
Here we introduce an example of analysis of halogen in a commercially available polyphenylene sulfide (PPS) resin using a combustion-ion chromatography system.
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Analysis of Hexafluorophosphate Ion in Electrolytic Solution for Lithium-Ion Rechargeable Batteries by Ion Chromatography
Lithium hexafluorophosphate is a common electrolyte used in rechargeable batteries. Here, we introduce an example of the analysis of hexafluorophosphate ion in the electrolytic solution of a lithium-ion rechargeable battery with the Shimadzu Prominence HIC-SP suppressor-type ion chromatograph.
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Analysis of the Decomposition Products of Lithium Hexafluorophosphate in the Electrolytic Solution of Lithium-Ion Rechargeable Batteries by Column-Switching Ion Chromatography
Here, we introduce an analysis of the decomposition products of lithium hexafluorophosphate used as the electrolytic solution in lithium-ion rechargeable batteries, in which we constructed a column-switching system using the Shimadzu Prominence HIC-SP ion chromatography system.
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Volume 13 of FTIR Talk Letter
Topics in this issue: Using Infrared Detectors (MCT Detector), Spectral Emissivity Measurement Using FTIR Spectrophotometry, Qualitative Analysis of Rubber Constituents.
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Volume 14 of FTIR Talk Letter
Topics in this issue: Integrating Sphere, The ABCs of Measurement Methods: KBr Pellet Method, Kbr window pane
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Volume 15 of FTIR Talk Letter
Topics in this issue: Fourier Transform and Apodization, ABCs of the FTIR Measurement Methods: Liquid Membrane Method, Questions about ripples in baseline and interference fringes.
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Volume 16 of FTIR Talk Letter
Topics in this issue: Structures and methods for selecting a gas cell used for measurement of gas samples, Measurement method ABCs: Diffuse Reflectance Method, ATR Measurement Attachment series accessories.
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Volume 3 of UV Talk Letter
Topics in this issue: the monochromator, an important part of the spectrophotometer that was explained in UV TALK LETTER Vol. 2 "The Structure of a Spectrophotometer", Application: Color measurement, Liquid sample spectrum measurement.
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Volume 4 of UV Talk Letter
Topics in this issue: Different types of detectors that convert light to electric signals, Application: Cell types and their selection, What is Validation?
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Volume 5 of UV Talk Letter
Topics in this issue: Integrating spheres, Application: Solar Transmittance/Solar Reflectance Measurement, What Is the Difference Between Relative Reflection and Absolute Reflection?
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Volume 6 of UV Talk Letter
Topics in this issue: Light sources for spectrophotometers, Measuring Solar Transmittance and Solar Reflectance, Part 2, Application: Cell Types and Their Selction, Minimum Limit of Quantitation
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Volume 7 of UV Talk Letter
Topics in this issue: BioSpec-nano Life Science UV-VIS Spectrophotometer, Application: Nucleic Acid Thermal Stability Analysis (Tm Analysis), Question: What types of colorimetric determination methods are there for proteins?
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AA Application - Trace Analysis of Palladium Using Electrothermal Atomization
Here, using the Nexera UHPLC (Ultra High Performance Liquid Chromatography) System, and the Shim-pack XR-ODS III and Phenomenex Kinetex C18 high speed, high-resolution columns, we introduce examples of ultra-high-speed analysis and ultra-high-resolution analysis of nucleic acid-related compounds.
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Nexera Application - High Speed, High Resolution Analysis (Part 40) Analysis of Nucleobases, Nucleosides, and Nucleotides by the Nexera UHPLC System
Here, we introduce an example of ultra-high-speed analysis of clobazam and cibenzoline in serum using the Nexera UHPLC (Ultra High Performance Liquid Chromatography) system and the Shim-pack XR-ODSIII high-speed separation column.
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Post-column pH Buffered Electric Conductivity Detection of Organic Acids with Ultra High Performance Liquid Chromatography (Japan)
Here, we introduce examples of Pd trace analysis using the graphite furnace atomization method with the organic solvents isopropanol (IPA) and n-methylpyrrolidone (NMP), both strongly compatible with water and easy to use.
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GCMS Application - GCMS High Sensitivity Technology Using Overdrive Lens (Japan)
For highly sensitive and selective determination of organic acids, “Postcolumn pH-buffered electric conductivity detection” was developed by Shimadzu Corporation. After separating organic acids by ionexclusion column, pH-buffered solution is added to the column eluent constantly in order to utterly ionize the organic acids which are weak anions. This technique greatly improves the selectivity of organic acids in complicated samples.
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Nexera Application - High Speed, High Resolution Analysis (Part 39) Analysis of Aflatoxins in Food by Nexera
The effects of increased signal intensity and improved signal-to-noise ratio (S/N) were investigated by eliminating noise with the installation of an overdrive lens in the GCMS-QP2010 Ultra, and verifying the effectiveness using simulation and experimentation by GC-MS. The results confirmed that S/N improved through application of the optimum voltage on the overdrive lens. In addition, it was verified from the results of analysis of 5 ppb chlorpyrifos-methyl by the full-scan method that high-sensitivity analysis is possible using the GCMS-QP2010 Ultra equipped with this technology.
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GC-MS High Sensitivity Technology Using Overdrive Lens
The effects of increased signal intensity and improved signal-to-noise ratio (S/N) were investigated by eliminating noise with the installation of an overdrive lens in the GCMS-QP2010 Ultra, and verifying the effectiveness using simulation and experimentation by GC-MS. The results confirmed that S/N improved through application of the optimum voltage on the overdrive lens. In addition, it was verified from the results of analysis of 5 ppb chlorpyrifos-methyl by the full-scan method that high-sensitivity analysis is possible using the GCMS-QP2010 Ultra equipped with this technology.
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Development of Green Technologies in GCMS-QP2010 Ultra
There has been an increasing interest in reducing running costs and environmental stress in many analytical laboratories using gas chromatograph-mass spectrometers in the fields of food, forensic, life science, environment, and the like. To respond such interest, we have developed two technologies for lower power and carrier gas consumption: “Advanced Scanning Speed Protocol” (ASSPTM) necessary for the Fast-GC/MS method and “Ecology Mode” for saving energy in analysis standby mode. The analysis of organic acids in urine for evaluation showed that the use of the Fast-GC/MS with ASSPTM reduced helium gas by 90 percent and power by 66 percent, compared with our previous model, while retaining performance for analysis. An evaluation test in analysis standby mode confirmed that the instrument with the Ecology Mode reduced carrier gas and power consumption, by 60 percent and 36.5 percent, respectively. These results demonstrate that our GCMS-QP2010 Ultra has environmentally friendly features and ensures lower running costs.
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GCMS-QP2010 Ultra Application - Analysis of Electrolyte Solution in Lithium Ion Rechargeable Battery (LIRB) and Evolved Gas from LIRB (Japan)
The electrolyte solution in a lithium ion rechargeable battery consists of an organic solvent (consisting chiefly of carbonates), the electrolyte, and additives. Evaluation of the electrolyte solution and analysis of its deterioration due to repetitive charge/discharge cycles is important for the further development of lithium ion rechargeable battery. GC-MS is applicable for composition analysis of the electrolyte solution, and for analysis of components in the electrolyte solution that have been generated due to repeated charging and discharging. This application news introduces examples of analysis of the electrolyte solution of a lithium ion rechargeable battery and of the gas evolved from a cell that has been maintained at a high temperature.
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Nexera Application - High Speed, High Resolution Analysis (Part 38) Analysis of Artificial Colorings with the Nexera UHPLC System
Here we introduce an example of ultra-high-speed analysis of aflatoxins in food using a combination of the Prominence RF-20Axs high-sensitivity fluorescence detector and the Nexera Ultra High Performance LC system, in which the aflatoxins B1, B2, G1, and G2 are analyzed using direct high-sensitivity fluorescence detection without conducting TFA derivatization.
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LCMS-8030/8040 Application - Analysis of Golf Course Pesticides Using Triple Quadrupole LC/MS/MS (LCMS-8030) (Japan)
In this issue, we present an example of ultra-high-speed analysis using the Nexera UHPLC (Ultra High Performance Liquid Chromatography) System.
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HPLC Application - Application of Evaporative Light-Scattering Detector (Part 10) High Speed Analysis of Glucosylceramide
This Application News introduces examples of analysis of the electrolyte solution of a lithium ion rechargeable battery and of the gas evolved from a cell that has been maintained at a high temeprature.
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Long-term Automated HPLC Analysis of Microdialysis Samples from Multiple Freely Moving Animals
Online microdialysis integrated with real-time HPLC analysis is a powerful tool for providing information on the circadian pacemaker, especially in combination with molecular and pharmacological tools. Other applications of this system are certainly possible for the long-term in-vivo analysis of various biological substances in freely moving animals. To access the full issue of LC World talk, please click <a href="http://www2.shimadzu.com/lc_worldtalk/" target="_blank">here</a>.
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Melamine Analysis Using Triple Quad LCMS-8030
This fast and reliable LC/MS/MS method for melamine and cyanuric acid is over five times faster than conventional methods for these compounds, with a run time under 60 seconds. The simple sample preparation involves only dilution and a one-step solvent extraction and can be automated for high-throughput sample analysis. The sensitivity is more than adequate to meet regulatory requirements while the speed and reliability enable rapid analysis of large numbers of samples. To access the full issue of LC World talk, please click <a href="http://www2.shimadzu.com/lc_worldtalk/" target="_blank">here</a>.
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Impurities Analysis in Pharmaceuticals: FDA Regulations on Genotoxic Impurities in Pharmaceuticals
The FDA draft guidance was issued to cover the verification of the genotoxicity and carcinogenicity of ultra-trace impurities in new pharmaceuticals, investigational new drugs, and generic pharmaceuticals by structural analysis of ultra-trace impurities. This demands structural analysis of ultra-trace impurities in a 200 mg daily drug dose at approximately 150 times lower levels than under the ICH guidelines. LCMS-IT-TOF analysis, which provides precise mass measurement and MSn spectra, is an effective method for the structural analysis of ultra-trace impurities. To access the full issue of LC World talk, please click <a href="http://www2.shimad