Pesticide, Mycotoxins & Other Contaminants


 
A Novel Method for Pre-Column Derivatization of Aflatoxin B1, B2, G1, and G2 Prior to HPLC Analysis using the XcelVap® System as a Thermostatically-Controlled Heated Water Chamber


Aflatoxins, a mold largely produced by Aspergillus flavus and Aspergillus parasiticus1 are commonly tested mycotoxins found naturally in a wide range of agriculture crops and food products. Due to their harmful effects on human health, animal health, and global trade, aflatoxins are regulated in most countries and have established global limits in a wide variety of matrices2. Regulations for the maximum limits vary for the reported aflatoxin B1 and total aflatoxins (sum of B1, B2, G1, and G2); however, most countries importing food and agriculture products perform testing to approve the safety of products. Testing may often reveal aflatoxin levels above the maximum limits allowed, creating a trade restriction for certain agriculture and food products from certain countries3. The tests are performed according to their sampling methods and the results are measured against their established limits.

This application focuses on the successful use of general equipment to accurately detect and report a linear seven-point calibration curve of aflatoxin B1, B2, G1, and G2 using the XcelVap as a thermostatically-controlled heated water chamber for derivatization.

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QuEChERS Method: AOAC Official Method 2007.01 (PDF-this will leave our site)

EPA sets pesticide tolerance levels, but FDA and USDA enforce those levels. (this link will leave our site)



QuEChERS Method: EN 15662 (PDF-this will leave our site)





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